Restriction map for
pFN21AE4545
(Linear) (Six-base) MAPSORT of: KIEE4545.seq Check: 6453 from: 1 to: 1905
KIEE4545.orf
With 182 enzymes: SgfI *
March 22, 2012 18:19 ..
AccI GT'mk_AC
Cuts at: 0 737 1637 1905
Size: 737 900 268
Fragments arranged by size:
900 737 268
Acc65I G'GTAC_C
Cuts at: 0 1535 1905
Size: 1535 370
AclI AA'CG_TT
Cuts at: 0 1613 1905
Size: 1613 292
AfeI AGC'GCT
Cuts at: 0 1768 1905
Size: 1768 137
AflIII A'CryG_T
Cuts at: 0 221 511 1652 1905
Size: 221 290 1141 253
Fragments arranged by size:
1141 290 253 221
AhdI GACnn_n'nnGTC
Cuts at: 0 618 710 1905
Size: 618 92 1195
Fragments arranged by size:
1195 618 92
AleI CACnn'nnGTG
Cuts at: 0 1651 1905
Size: 1651 254
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1462 1905
Size: 1462 443
AlwNI CAG_nnn'CTG
Cuts at: 0 1779 1905
Size: 1779 126
ApaI G_GGCC'C
Cuts at: 0 61 80 90 690 969 1905
Size: 61 19 10 600 279 936
Fragments arranged by size:
936 600 279 61 19 10
ApaLI G'TGCA_C
Cuts at: 0 1114 1905
Size: 1114 791
AvaI C'yCGr_G
Cuts at: 0 73 1905
Size: 73 1832
BanI G'GyrC_C
Cuts at: 0 678 850 1438 1535 1753 1905
Size: 678 172 588 97 218 152
Fragments arranged by size:
678 588 218 172 152 97
BanII G_rGCy'C
Cuts at: 0 61 80 90 690 969 1824 1905
Size: 61 19 10 600 279 855 81
Fragments arranged by size:
855 600 279 81 61 19 10
BbeI G_GCGC'C
Cuts at: 0 854 1905
Size: 854 1051
BbsI GAAGACnn'nnnn_
Cuts at: 0 414 569 1068 1398 1905
Size: 414 155 499 330 507
Fragments arranged by size:
507 499 414 330 155
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1863 1866 1897 1900 1905
Size: 1863 3 31 3 5
Fragments arranged by size:
1863 31 5 3 3
BglI GCCn_nnn'nGGC
Cuts at: 0 113 583 1905
Size: 113 470 1322
Fragments arranged by size:
1322 470 113
BglII A'GATC_T
Cuts at: 0 326 548 1331 1905
Size: 326 222 783 574
Fragments arranged by size:
783 574 326 222
BlpI GC'TnA_GC
Cuts at: 0 1763 1905
Size: 1763 142
Bme1580I G_kGCm'C
Cuts at: 0 61 80 90 690 969 1118 1356 1756
Size: 61 19 10 600 279 149 238 400
Cuts at: 1756 1905
Size: 149
Fragments arranged by size:
600 400 279 238 149 149 61 19
10
BmrI ACTGGGnnnn_n'
Cuts at: 0 705 1855 1905
Size: 705 1150 50
Fragments arranged by size:
1150 705 50
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 694 1905
Size: 694 1211
Bpu10I CC'TnA_GC
Cuts at: 0 1823 1905
Size: 1823 82
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 675 862 1905
Size: 675 187 1043
Fragments arranged by size:
1043 675 187
BsaI GGTCTCn'nnnn_
Cuts at: 0 151 1246 1905
Size: 151 1095 659
Fragments arranged by size:
1095 659 151
BsaAI yAC'GTr
Cuts at: 0 807 1653 1905
Size: 807 846 252
Fragments arranged by size:
846 807 252
BsaHI Gr'CG_yC
Cuts at: 0 851 1019 1905
Size: 851 168 886
Fragments arranged by size:
886 851 168
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1276 1285 1729 1905
Size: 1276 9 444 176
Fragments arranged by size:
1276 444 176 9
BseYI C'CCAG_C
Cuts at: 0 333 725 897 1682 1784 1905
Size: 333 392 172 785 102 121
Fragments arranged by size:
785 392 333 172 121 102
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1705 1905
Size: 1705 200
BsiEI CG_ry'CG
Cuts at: 0 135 1905
Size: 135 1770
BsiHKAI G_wGCw'C
Cuts at: 0 1118 1824 1905
Size: 1118 706 81
Fragments arranged by size:
1118 706 81
Bsp1286I G_dGCh'C
Cuts at: 0 61 80 90 690 969 1118 1356 1756
Size: 61 19 10 600 279 149 238 400
Cuts at: 1756 1824 1905
Size: 68 81
Fragments arranged by size:
600 400 279 238 149 81 68 61
19 10
BspHI T'CATG_A
Cuts at: 0 344 1905
Size: 344 1561
BsrDI GCAATG_nn'
Cuts at: 0 1517 1905
Size: 1517 388
BsrFI r'CCGG_y
Cuts at: 0 352 617 1279 1905
Size: 352 265 662 626
Fragments arranged by size:
662 626 352 265
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1113 1905
Size: 1113 792
BstEII G'GTnAC_C
Cuts at: 0 702 1905
Size: 702 1203
BstXI CCAn_nnnn'nTGG
Cuts at: 0 960 1029 1905
Size: 960 69 876
Fragments arranged by size:
960 876 69
BstYI r'GATC_y
Cuts at: 0 326 548 1331 1905
Size: 326 222 783 574
Fragments arranged by size:
783 574 326 222
Bsu36I CC'TnA_GG
Cuts at: 0 908 1741 1905
Size: 908 833 164
Fragments arranged by size:
908 833 164
BtgI C'CryG_G
Cuts at: 0 110 206 410 767 1168 1379 1660 1676
Size: 110 96 204 357 401 211 281 16
Cuts at: 1676 1757 1905
Size: 81 148
Fragments arranged by size:
401 357 281 211 204 148 110 96
81 16
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 51 1143 1905
Size: 51 1092 762
Fragments arranged by size:
1092 762 51
BtsI GCAGTG_nn'
Cuts at: 0 788 1905
Size: 788 1117
ClaI AT'CG_AT
Cuts at: 0 1370 1905
Size: 1370 535
DrdI GACnn_nn'nnGTC
Cuts at: 0 1834 1863 1905
Size: 1834 29 42
Fragments arranged by size:
1834 42 29
EaeI y'GGCC_r
Cuts at: 0 92 132 140 516 584 1430 1905
Size: 92 40 8 376 68 846 475
Fragments arranged by size:
846 475 376 92 68 40 8
EagI C'GGCC_G
Cuts at: 0 132 1905
Size: 132 1773
EarI CTCTTCn'nnn_
Cuts at: 0 4 1905
Size: 4 1901
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 272 544 1905
Size: 272 272 1361
Fragments arranged by size:
1361 272 272
EcoICRI GAG'CTC
Cuts at: 0 1822 1905
Size: 1822 83
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 694 1905
Size: 694 1211
EcoO109I rG'GnC_Cy
Cuts at: 0 57 58 86 87 686 687 902 929
Size: 57 1 28 1 599 1 215 27
Cuts at: 929 965 966 1797 1842 1905
Size: 36 1 831 45 63
Fragments arranged by size:
831 599 215 63 57 45 36 28
27 1 1 1 1
HaeII r_GCGC'y
Cuts at: 0 17 231 854 1770 1905
Size: 17 214 623 916 135
Fragments arranged by size:
916 623 214 135 17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 604 636 696 728 1411 1443 1905
Size: 604 32 60 32 683 32 462
Fragments arranged by size:
683 604 462 60 32 32 32
KasI G'GCGC_C
Cuts at: 0 850 1905
Size: 850 1055
KpnI G_GTAC'C
Cuts at: 0 1539 1905
Size: 1539 366
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 279 1905
Size: 279 1626
MscI TGG'CCA
Cuts at: 0 518 586 1905
Size: 518 68 1319
Fragments arranged by size:
1319 518 68
MslI CAynn'nnrTG
Cuts at: 0 448 1183 1651 1905
Size: 448 735 468 254
Fragments arranged by size:
735 468 448 254
MspA1I CmG'CkG
Cuts at: 0 1170 1413 1436 1584 1632 1719 1905
Size: 1170 243 23 148 48 87 186
Fragments arranged by size:
1170 243 186 148 87 48 23
NaeI GCC'GGC
Cuts at: 0 354 1905
Size: 354 1551
NarI GG'CG_CC
Cuts at: 0 851 1905
Size: 851 1054
NcoI C'CATG_G
Cuts at: 0 410 1379 1676 1757 1905
Size: 410 969 297 81 148
Fragments arranged by size:
969 410 297 148 81
NgoMIV G'CCGG_C
Cuts at: 0 352 1905
Size: 352 1553
NotI GC'GGCC_GC
Cuts at: 0 132 1905
Size: 132 1773
NspI r_CATG'y
Cuts at: 0 515 1905
Size: 515 1390
PasI CC'CwG_GG
Cuts at: 0 503 978 1905
Size: 503 475 927
Fragments arranged by size:
927 503 475
PciI A'CATG_T
Cuts at: 0 511 1905
Size: 511 1394
PflMI CCAn_nnn'nTGG
Cuts at: 0 1682 1809 1905
Size: 1682 127 96
Fragments arranged by size:
1682 127 96
PpuMI rG'GwC_Cy
Cuts at: 0 902 929 1905
Size: 902 27 976
Fragments arranged by size:
976 902 27
PspOMI G'GGCC_C
Cuts at: 0 57 76 86 686 965 1905
Size: 57 19 10 600 279 940
Fragments arranged by size:
940 600 279 57 19 10
PstI C_TGCA'G
Cuts at: 0 1104 1905
Size: 1104 801
PvuII CAG'CTG
Cuts at: 0 1413 1905
Size: 1413 492
SacI G_AGCT'C
Cuts at: 0 1824 1905
Size: 1824 81
SacII CC_GC'GG
Cuts at: 0 1171 1905
Size: 1171 734
SanDI GG'GwC_CC
Cuts at: 0 902 929 1905
Size: 902 27 976
Fragments arranged by size:
976 902 27
SapI GCTCTTCn'nnn_
Cuts at: 0 4 1905
Size: 4 1901
SexAI A'CCwGG_T
Cuts at: 0 473 1905
Size: 473 1432
SfcI C'TryA_G
Cuts at: 0 30 1100 1905
Size: 30 1070 805
Fragments arranged by size:
1070 805 30
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 113 1905
Size: 113 1792
SfoI GGC'GCC
Cuts at: 0 852 1905
Size: 852 1053
SmaI CCC'GGG
Cuts at: 0 75 1905
Size: 75 1830
SmlI C'TyrA_G
Cuts at: 0 654 877 1905
Size: 654 223 1028
Fragments arranged by size:
1028 654 223
SrfI GCCC'GGGC
Cuts at: 0 75 1905
Size: 75 1830
StuI AGG'CCT
Cuts at: 0 395 1459 1905
Size: 395 1064 446
Fragments arranged by size:
1064 446 395
StyI C'CwwG_G
Cuts at: 0 95 410 1124 1379 1676 1748 1757 1905
Size: 95 315 714 255 297 72 9 148
Fragments arranged by size:
714 315 297 255 148 95 72 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 717 1476 1905
Size: 717 759 429
Fragments arranged by size:
759 717 429
TatI w'GTAC_w
Cuts at: 0 782 1619 1905
Size: 782 837 286
Fragments arranged by size:
837 782 286
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 662 694 764 796 1905
Size: 662 32 70 32 1109
Fragments arranged by size:
1109 662 70 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 579 1755 1905
Size: 579 1176 150
Fragments arranged by size:
1176 579 150
XmaI C'CCGG_G
Cuts at: 0 73 1905
Size: 73 1832
Enzymes that do cut:
AccI Acc65I AclI AfeI AflIII AhdI AleI AlfI
AlwNI ApaI ApaLI AvaI BanI BanII BbeI BbsI
BcgI BglI BglII BlpI Bme1580I BmrI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspHI BsrDI BsrFI BstAPI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I HaeII Hin4I
KasI KpnI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NotI NspI PasI PciI PflMI PpuMI
PspOMI PstI PvuII SacI SacII SanDI SapI SexAI
SfcI SfiI SfoI SmaI SmlI SrfI StuI StyI
TaqII TatI TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AcuI AflII AgeI AloI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbvCI BciVI BclI
BfrBI BmgBI BmtI BplI BsaBI BsaWI BsaXI BsiWI
BsmI BsmBI BspEI BspMI BsrBI BsrGI BssHII BssSI
BstBI BstZ17I CspCI DraI DraIII EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI MfeI
MluI NdeI NheI NruI NsiI PacI PfoI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SalI SbfI ScaI SgfI SgrAI SnaBI SpeI SphI
SspI SwaI Tth111I XbaI XhoI XmnI ZraI