Restriction map for
pFN21AE4542
(Linear) (Six-base) MAPSORT of: KIEE4542.seq Check: 7298 from: 1 to: 1878
KIEE4542.orf
With 182 enzymes: SgfI *
March 22, 2012 18:19 ..
AatII G_ACGT'C
Cuts at: 0 368 755 1878
Size: 368 387 1123
Fragments arranged by size:
1123 387 368
AccI GT'mk_AC
Cuts at: 0 601 728 815 1878
Size: 601 127 87 1063
Fragments arranged by size:
1063 601 127 87
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 475 1878
Size: 475 1403
AfeI AGC'GCT
Cuts at: 0 119 1878
Size: 119 1759
AleI CACnn'nnGTG
Cuts at: 0 161 1878
Size: 161 1717
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 731 1878
Size: 731 1147
AlwNI CAG_nnn'CTG
Cuts at: 0 342 1608 1836 1878
Size: 342 1266 228 42
Fragments arranged by size:
1266 342 228 42
ApoI r'AATT_y
Cuts at: 0 1083 1878
Size: 1083 795
AvaI C'yCGr_G
Cuts at: 0 1694 1878
Size: 1694 184
BanI G'GyrC_C
Cuts at: 0 400 836 1878
Size: 400 436 1042
Fragments arranged by size:
1042 436 400
BanII G_rGCy'C
Cuts at: 0 248 434 476 536 548 1172 1184 1211
Size: 248 186 42 60 12 624 12 27
Cuts at: 1211 1238 1250 1277 1465 1878
Size: 27 12 27 188 413
Fragments arranged by size:
624 413 248 188 186 60 42 27
27 27 12 12 12
BbsI GAAGACnn'nnnn_
Cuts at: 0 463 1878
Size: 463 1415
BbvCI CC'TCA_GC
Cuts at: 0 1806 1878
Size: 1806 72
BclI T'GATC_A
Cuts at: 0 1334 1416 1878
Size: 1334 82 462
Fragments arranged by size:
1334 462 82
BglI GCCn_nnn'nGGC
Cuts at: 0 471 1878
Size: 471 1407
BlpI GC'TnA_GC
Cuts at: 0 1576 1840 1878
Size: 1576 264 38
Fragments arranged by size:
1576 264 38
Bme1580I G_kGCm'C
Cuts at: 0 405 841 1694 1878
Size: 405 436 853 184
Fragments arranged by size:
853 436 405 184
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1257 1878
Size: 1257 621
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 110 241 517 580 1225 1449 1681 1725
Size: 110 131 276 63 645 224 232 44
Cuts at: 1725 1878
Size: 153
Fragments arranged by size:
645 276 232 224 153 131 110 63
44
Bpu10I CC'TnA_GC
Cuts at: 0 1806 1878
Size: 1806 72
BsaI GGTCTCn'nnnn_
Cuts at: 0 69 1537 1878
Size: 69 1468 341
Fragments arranged by size:
1468 341 69
BsaAI yAC'GTr
Cuts at: 0 732 1878
Size: 732 1146
BsaHI Gr'CG_yC
Cuts at: 0 365 481 752 1878
Size: 365 116 271 1126
Fragments arranged by size:
1126 365 271 116
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 169 199 1415 1445 1878
Size: 169 30 1216 30 433
Fragments arranged by size:
1216 433 169 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1 571 1008 1495 1878
Size: 1 570 437 487 383
Fragments arranged by size:
570 487 437 383 1
BseYI C'CCAG_C
Cuts at: 0 282 541 1255 1580 1878
Size: 282 259 714 325 298
Fragments arranged by size:
714 325 298 282 259
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 805 1878
Size: 805 1073
BsiHKAI G_wGCw'C
Cuts at: 0 248 434 536 1445 1465 1846 1878
Size: 248 186 102 909 20 381 32
Fragments arranged by size:
909 381 248 186 102 32 20
BsmBI CGTCTCn'nnnn_
Cuts at: 0 372 1878
Size: 372 1506
Bsp1286I G_dGCh'C
Cuts at: 0 248 405 434 476 536 548 841 1172
Size: 248 157 29 42 60 12 293 331
Cuts at: 1172 1184 1211 1238 1250 1277 1445 1465 1694
Size: 12 27 27 12 27 168 20 229
Cuts at: 1694 1846 1878
Size: 152 32
Fragments arranged by size:
331 293 248 229 168 157 152 60
42 32 29 27 27 27 20 12
12 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 140 1844 1878
Size: 140 1704 34
Fragments arranged by size:
1704 140 34
BsrBI CCG'CTC
Cuts at: 0 1739 1878
Size: 1739 139
BsrDI GCAATG_nn'
Cuts at: 0 453 1878
Size: 453 1425
BsrFI r'CCGG_y
Cuts at: 0 377 1878
Size: 377 1501
BsrGI T'GTAC_A
Cuts at: 0 176 1878
Size: 176 1702
BstAPI GCAn_nnn'nTGC
Cuts at: 0 321 342 1878
Size: 321 21 1536
Fragments arranged by size:
1536 321 21
BstXI CCAn_nnnn'nTGG
Cuts at: 0 265 1073 1659 1878
Size: 265 808 586 219
Fragments arranged by size:
808 586 265 219
BstYI r'GATC_y
Cuts at: 0 294 683 1878
Size: 294 389 1195
Fragments arranged by size:
1195 389 294
BstZ17I GTA'TAC
Cuts at: 0 602 1878
Size: 602 1276
BtgI C'CryG_G
Cuts at: 0 654 1434 1878
Size: 654 780 444
Fragments arranged by size:
780 654 444
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1278 1878
Size: 1278 600
BtsI GCAGTG_nn'
Cuts at: 0 340 793 989 1878
Size: 340 453 196 889
Fragments arranged by size:
889 453 340 196
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 911 946 1878
Size: 911 35 932
Fragments arranged by size:
932 911 35
EaeI y'GGCC_r
Cuts at: 0 74 776 1617 1770 1866 1878
Size: 74 702 841 153 96 12
Fragments arranged by size:
841 702 153 96 74 12
EarI CTCTTCn'nnn_
Cuts at: 0 884 1540 1714 1753 1878
Size: 884 656 174 39 125
Fragments arranged by size:
884 656 174 125 39
EcoICRI GAG'CTC
Cuts at: 0 246 432 534 1463 1878
Size: 246 186 102 929 415
Fragments arranged by size:
929 415 246 186 102
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 110 241 475 517 580 1225 1449 1681
Size: 110 131 234 42 63 645 224 232
Cuts at: 1681 1725 1878
Size: 44 153
Fragments arranged by size:
645 234 232 224 153 131 110 63
44 42
EcoO109I rG'GnC_Cy
Cuts at: 0 287 626 1605 1780 1802 1833 1878
Size: 287 339 979 175 22 31 45
Fragments arranged by size:
979 339 287 175 45 31 22
HaeII r_GCGC'y
Cuts at: 0 121 1878
Size: 121 1757
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 279 311 1806 1838 1878
Size: 279 32 1495 32 40
Fragments arranged by size:
1495 279 40 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1054 1878
Size: 1054 824
MscI TGG'CCA
Cuts at: 0 76 778 1619 1868 1878
Size: 76 702 841 249 10
Fragments arranged by size:
841 702 249 76 10
MslI CAynn'nnrTG
Cuts at: 0 161 1071 1315 1878
Size: 161 910 244 563
Fragments arranged by size:
910 563 244 161
MspA1I CmG'CkG
Cuts at: 0 225 312 541 1878
Size: 225 87 229 1337
Fragments arranged by size:
1337 229 225 87
NspI r_CATG'y
Cuts at: 0 1594 1878
Size: 1594 284
PasI CC'CwG_GG
Cuts at: 0 469 965 1512 1878
Size: 469 496 547 366
Fragments arranged by size:
547 496 469 366
PfoI T'CCnGG_A
Cuts at: 0 359 1175 1878
Size: 359 816 703
Fragments arranged by size:
816 703 359
PpuMI rG'GwC_Cy
Cuts at: 0 287 626 1833 1878
Size: 287 339 1207 45
Fragments arranged by size:
1207 339 287 45
PshAI GACnn'nnGTC
Cuts at: 0 931 1878
Size: 931 947
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 160 192 1878
Size: 160 32 1686
Fragments arranged by size:
1686 160 32
PstI C_TGCA'G
Cuts at: 0 710 1670 1878
Size: 710 960 208
Fragments arranged by size:
960 710 208
PvuII CAG'CTG
Cuts at: 0 225 312 1878
Size: 225 87 1566
Fragments arranged by size:
1566 225 87
SacI G_AGCT'C
Cuts at: 0 248 434 536 1465 1878
Size: 248 186 102 929 413
Fragments arranged by size:
929 413 248 186 102
SanDI GG'GwC_CC
Cuts at: 0 287 626 1878
Size: 287 339 1252
Fragments arranged by size:
1252 339 287
SapI GCTCTTCn'nnn_
Cuts at: 0 1714 1878
Size: 1714 164
SfcI C'TryA_G
Cuts at: 0 706 1666 1878
Size: 706 960 212
Fragments arranged by size:
960 706 212
SmaI CCC'GGG
Cuts at: 0 1696 1878
Size: 1696 182
SnaBI TAC'GTA
Cuts at: 0 732 1878
Size: 732 1146
StyI C'CwwG_G
Cuts at: 0 846 1446 1620 1878
Size: 846 600 174 258
Fragments arranged by size:
846 600 258 174
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 504 1878
Size: 504 1374
TatI w'GTAC_w
Cuts at: 0 176 1878
Size: 176 1702
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 729 761 1087 1119 1415 1447 1667 1699
Size: 729 32 326 32 296 32 220 32
Cuts at: 1699 1878
Size: 179
Fragments arranged by size:
729 326 296 220 179 32 32 32
32
XbaI T'CTAG_A
Cuts at: 0 999 1878
Size: 999 879
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 492 927 1878
Size: 492 435 951
Fragments arranged by size:
951 492 435
XmaI C'CCGG_G
Cuts at: 0 1694 1878
Size: 1694 184
ZraI GAC'GTC
Cuts at: 0 366 753 1878
Size: 366 387 1125
Fragments arranged by size:
1125 387 366
Enzymes that do cut:
AatII AccI AcuI AfeI AleI AlfI AlwNI ApoI
AvaI BanI BanII BbsI BbvCI BclI BglI BlpI
Bme1580I BplI BpmI Bpu10I BsaI BsaAI BsaHI BsaXI
BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrBI
BsrDI BsrFI BsrGI BstAPI BstXI BstYI BstZ17I BtgI
BtgZI BtsI CspCI EaeI EarI EcoICRI Eco57MI EcoO109I
HaeII Hin4I MmeI MscI MslI MspA1I NspI PasI
PfoI PpuMI PshAI PsrI PstI PvuII SacI SanDI
SapI SfcI SmaI SnaBI StyI TaqII TatI TstI
XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AflII AflIII AgeI AhdI AloI
ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BcgI BciVI BfrBI BglII BmgBI BmrI BmtI
BpuEI BsaBI BsaWI BsiEI BsiWI BsmI BspEI BspHI
BssHII BssSI BstBI BstEII Bsu36I ClaI DraI DraIII
DrdI EagI EciI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI KasI KpnI MfeI
MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PciI PflMI PmeI PmlI PpiI
PsiI PspOMI PspXI PvuI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SpeI
SphI SrfI SspI StuI SwaI Tth111I XhoI XmnI