Restriction map for
pFN21AE4541
(Linear) (Six-base) MAPSORT of: KIEE4541.seq Check: 9404 from: 1 to: 1869
KIEE4541.orf
With 182 enzymes: SgfI *
March 22, 2012 18:19 ..
Acc65I G'GTAC_C
Cuts at: 0 1773 1869
Size: 1773 96
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 281 1507 1585 1825 1869
Size: 281 1226 78 240 44
Fragments arranged by size:
1226 281 240 78 44
AfeI AGC'GCT
Cuts at: 0 1644 1869
Size: 1644 225
AleI CACnn'nnGTG
Cuts at: 0 355 1869
Size: 355 1514
AlwNI CAG_nnn'CTG
Cuts at: 0 33 972 1869
Size: 33 939 897
Fragments arranged by size:
939 897 33
ApaI G_GGCC'C
Cuts at: 0 1277 1869
Size: 1277 592
ApoI r'AATT_y
Cuts at: 0 1029 1869
Size: 1029 840
AvaI C'yCGr_G
Cuts at: 0 385 1869
Size: 385 1484
BamHI G'GATC_C
Cuts at: 0 1664 1869
Size: 1664 205
BanI G'GyrC_C
Cuts at: 0 326 1146 1773 1869
Size: 326 820 627 96
Fragments arranged by size:
820 627 326 96
BanII G_rGCy'C
Cuts at: 0 103 484 1277 1869
Size: 103 381 793 592
Fragments arranged by size:
793 592 381 103
BbsI GAAGACnn'nnnn_
Cuts at: 0 1274 1869
Size: 1274 595
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 405 431 439 465 1810 1844 1869
Size: 405 26 8 26 1345 34 25
Fragments arranged by size:
1345 405 34 26 26 25 8
BglII A'GATC_T
Cuts at: 0 818 1046 1296 1869
Size: 818 228 250 573
Fragments arranged by size:
818 573 250 228
BlpI GC'TnA_GC
Cuts at: 0 800 1796 1869
Size: 800 996 73
Fragments arranged by size:
996 800 73
Bme1580I G_kGCm'C
Cuts at: 0 329 1151 1277 1869
Size: 329 822 126 592
Fragments arranged by size:
822 592 329 126
BmgBI CAC'GTC
Cuts at: 0 116 357 1869
Size: 116 241 1512
Fragments arranged by size:
1512 241 116
BsaAI yAC'GTr
Cuts at: 0 67 1869
Size: 67 1802
BsaBI GATnn'nnATC
Cuts at: 0 598 1582 1869
Size: 598 984 287
Fragments arranged by size:
984 598 287
BsaWI w'CCGG_w
Cuts at: 0 1409 1869
Size: 1409 460
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 655 685 1261 1291 1869
Size: 655 30 576 30 578
Fragments arranged by size:
655 578 576 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 679 1869
Size: 679 1190
BseYI C'CCAG_C
Cuts at: 0 456 910 919 981 1522 1869
Size: 456 454 9 62 541 347
Fragments arranged by size:
541 456 454 347 62 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1519 1742 1869
Size: 1519 223 127
Fragments arranged by size:
1519 223 127
BsiHKAI G_wGCw'C
Cuts at: 0 218 1064 1869
Size: 218 846 805
Fragments arranged by size:
846 805 218
Bsp1286I G_dGCh'C
Cuts at: 0 103 218 329 484 1064 1151 1277 1869
Size: 103 115 111 155 580 87 126 592
Fragments arranged by size:
592 580 155 126 115 111 103 87
BspEI T'CCGG_A
Cuts at: 0 1409 1869
Size: 1409 460
BsrDI GCAATG_nn'
Cuts at: 0 713 890 1088 1832 1869
Size: 713 177 198 744 37
Fragments arranged by size:
744 713 198 177 37
BsrFI r'CCGG_y
Cuts at: 0 412 1869
Size: 412 1457
BssSI C'ACGA_G
Cuts at: 0 1537 1646 1869
Size: 1537 109 223
Fragments arranged by size:
1537 223 109
BstAPI GCAn_nnn'nTGC
Cuts at: 0 33 1869
Size: 33 1836
BstEII G'GTnAC_C
Cuts at: 0 1506 1527 1869
Size: 1506 21 342
Fragments arranged by size:
1506 342 21
BstXI CCAn_nnnn'nTGG
Cuts at: 0 645 795 1869
Size: 645 150 1074
Fragments arranged by size:
1074 645 150
BstYI r'GATC_y
Cuts at: 0 818 830 848 1046 1296 1664 1869
Size: 818 12 18 198 250 368 205
Fragments arranged by size:
818 368 250 205 198 18 12
Bsu36I CC'TnA_GG
Cuts at: 0 298 1869
Size: 298 1571
BtgI C'CryG_G
Cuts at: 0 497 1673 1869
Size: 497 1176 196
Fragments arranged by size:
1176 497 196
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 990 1098 1869
Size: 990 108 771
Fragments arranged by size:
990 771 108
BtsI GCAGTG_nn'
Cuts at: 0 89 1245 1376 1869
Size: 89 1156 131 493
Fragments arranged by size:
1156 493 131 89
DraIII CAC_nnn'GTG
Cuts at: 0 660 1869
Size: 660 1209
DrdI GACnn_nn'nnGTC
Cuts at: 0 1462 1869
Size: 1462 407
EaeI y'GGCC_r
Cuts at: 0 53 105 275 359 536 684 974 1205
Size: 53 52 170 84 177 148 290 231
Cuts at: 1205 1547 1869
Size: 342 322
Fragments arranged by size:
342 322 290 231 177 170 148 84
53 52
EarI CTCTTCn'nnn_
Cuts at: 0 1483 1869
Size: 1483 386
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 298 1869
Size: 298 1571
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 281 1507 1585 1825 1869
Size: 281 1226 78 240 44
Fragments arranged by size:
1226 281 240 78 44
EcoNI CCTnn'n_nnAGG
Cuts at: 0 277 376 1537 1869
Size: 277 99 1161 332
Fragments arranged by size:
1161 332 277 99
EcoO109I rG'GnC_Cy
Cuts at: 0 183 381 1274 1851 1869
Size: 183 198 893 577 18
Fragments arranged by size:
893 577 198 183 18
HaeII r_GCGC'y
Cuts at: 0 131 1646 1869
Size: 131 1515 223
Fragments arranged by size:
1515 223 131
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 748 780 865 897 1261 1293 1699 1731
Size: 748 32 85 32 364 32 406 32
Cuts at: 1731 1869
Size: 138
Fragments arranged by size:
748 406 364 138 85 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1767 1869
Size: 1767 102
HindIII A'AGCT_T
Cuts at: 0 344 1869
Size: 344 1525
HpaI GTT'AAC
Cuts at: 0 1767 1869
Size: 1767 102
KpnI G_GTAC'C
Cuts at: 0 1777 1869
Size: 1777 92
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 338 1446 1869
Size: 338 1108 423
Fragments arranged by size:
1108 423 338
MscI TGG'CCA
Cuts at: 0 107 361 538 686 976 1869
Size: 107 254 177 148 290 893
Fragments arranged by size:
893 290 254 177 148 107
MslI CAynn'nnrTG
Cuts at: 0 355 643 904 992 1117 1588 1688 1869
Size: 355 288 261 88 125 471 100 181
Fragments arranged by size:
471 355 288 261 181 125 100 88
MspA1I CmG'CkG
Cuts at: 0 582 1552 1795 1869
Size: 582 970 243 74
Fragments arranged by size:
970 582 243 74
NcoI C'CATG_G
Cuts at: 0 497 1869
Size: 497 1372
NspI r_CATG'y
Cuts at: 0 637 909 1869
Size: 637 272 960
Fragments arranged by size:
960 637 272
PasI CC'CwG_GG
Cuts at: 0 372 1869
Size: 372 1497
PflMI CCAn_nnn'nTGG
Cuts at: 0 1348 1783 1869
Size: 1348 435 86
Fragments arranged by size:
1348 435 86
PfoI T'CCnGG_A
Cuts at: 0 812 1869
Size: 812 1057
PpuMI rG'GwC_Cy
Cuts at: 0 183 1869
Size: 183 1686
PspOMI G'GGCC_C
Cuts at: 0 1273 1869
Size: 1273 596
PstI C_TGCA'G
Cuts at: 0 38 127 587 1159 1869
Size: 38 89 460 572 710
Fragments arranged by size:
710 572 460 89 38
PvuII CAG'CTG
Cuts at: 0 1795 1869
Size: 1795 74
RsrII CG'GwC_CG
Cuts at: 0 1068 1869
Size: 1068 801
SapI GCTCTTCn'nnn_
Cuts at: 0 1483 1869
Size: 1483 386
SexAI A'CCwGG_T
Cuts at: 0 1502 1869
Size: 1502 367
SfcI C'TryA_G
Cuts at: 0 34 123 583 822 1155 1358 1869
Size: 34 89 460 239 333 203 511
Fragments arranged by size:
511 460 333 239 203 89 34
SnaBI TAC'GTA
Cuts at: 0 67 1869
Size: 67 1802
SphI G_CATG'C
Cuts at: 0 637 909 1869
Size: 637 272 960
Fragments arranged by size:
960 637 272
StuI AGG'CCT
Cuts at: 0 40 622 679 958 1543 1869
Size: 40 582 57 279 585 326
Fragments arranged by size:
585 582 326 279 57 40
StyI C'CwwG_G
Cuts at: 0 221 497 1475 1544 1655 1862 1869
Size: 221 276 978 69 111 207 7
Fragments arranged by size:
978 276 221 207 111 69 7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1301 1768 1869
Size: 1301 467 101
Fragments arranged by size:
1301 467 101
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 644 655 676 687 1869
Size: 644 11 21 11 1182
Fragments arranged by size:
1182 644 21 11 11
XbaI T'CTAG_A
Cuts at: 0 543 1869
Size: 543 1326
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 369 1614 1725 1869
Size: 369 1245 111 144
Fragments arranged by size:
1245 369 144 111
Enzymes that do cut:
Acc65I AcuI AfeI AleI AlwNI ApaI ApoI AvaI
BamHI BanI BanII BbsI BcgI BglII BlpI Bme1580I
BmgBI BsaAI BsaBI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspEI BsrDI BsrFI BssSI BstAPI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII DrdI
EaeI EarI EciI Eco57MI EcoNI EcoO109I HaeII Hin4I
HincII HindIII HpaI KpnI MmeI MscI MslI MspA1I
NcoI NspI PasI PflMI PfoI PpuMI PspOMI PstI
PvuII RsrII SapI SexAI SfcI SnaBI SphI StuI
StyI TaqII TstI XbaI XcmI
Enzymes that do not cut:
AarI AatII AccI AclI AflII AflIII AgeI AhdI
AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI
BbeI BbvCI BciVI BclI BfrBI BglI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaHI BsiEI BsiWI
BsmI BsmBI BspHI BspMI BsrBI BsrGI BssHII BstBI
BstZ17I ClaI CspCI DraI EagI EcoICRI EcoRI EcoRV
FalI FseI FspI FspAI KasI MfeI MluI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PvuI SacI SacII SalI SanDI SbfI ScaI SfiI
SfoI SgfI SgrAI SmaI SmlI SpeI SrfI SspI
SwaI TatI Tth111I XhoI XmaI XmnI ZraI