Restriction map for
pFN21AE4537
(Linear) (Six-base) MAPSORT of: KIEE4537.seq Check: 1520 from: 1 to: 1815
KIEE4537.orf
With 182 enzymes: SgfI *
March 22, 2012 18:18 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 125 834 1815
Size: 125 709 981
Fragments arranged by size:
981 709 125
AflII C'TTAA_G
Cuts at: 0 1662 1815
Size: 1662 153
AleI CACnn'nnGTG
Cuts at: 0 569 1815
Size: 569 1246
ApoI r'AATT_y
Cuts at: 0 593 859 1290 1341 1533 1815
Size: 593 266 431 51 192 282
Fragments arranged by size:
593 431 282 266 192 51
AvaI C'yCGr_G
Cuts at: 0 189 215 347 1370 1815
Size: 189 26 132 1023 445
Fragments arranged by size:
1023 445 189 132 26
BamHI G'GATC_C
Cuts at: 0 1272 1815
Size: 1272 543
BanI G'GyrC_C
Cuts at: 0 258 1815
Size: 258 1557
BanII G_rGCy'C
Cuts at: 0 92 1815
Size: 92 1723
BbsI GAAGACnn'nnnn_
Cuts at: 0 24 490 1815
Size: 24 466 1325
Fragments arranged by size:
1325 466 24
BciVI GTATCCnnnnn_n'
Cuts at: 0 1448 1815
Size: 1448 367
BglI GCCn_nnn'nGGC
Cuts at: 0 153 220 256 1815
Size: 153 67 36 1559
Fragments arranged by size:
1559 153 67 36
BlpI GC'TnA_GC
Cuts at: 0 1682 1815
Size: 1682 133
Bme1580I G_kGCm'C
Cuts at: 0 320 1815
Size: 320 1495
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1092 1112 1285 1815
Size: 1092 20 173 530
Fragments arranged by size:
1092 530 173 20
Bpu10I CC'TnA_GC
Cuts at: 0 407 1815
Size: 407 1408
BsaAI yAC'GTr
Cuts at: 0 567 1815
Size: 567 1248
BsaHI Gr'CG_yC
Cuts at: 0 242 1815
Size: 242 1573
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 14 44 343 373 1065 1095 1815
Size: 14 30 299 30 692 30 720
Fragments arranged by size:
720 692 299 30 30 30 14
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1012 1282 1815
Size: 1012 270 533
Fragments arranged by size:
1012 533 270
BseYI C'CCAG_C
Cuts at: 0 18 201 1815
Size: 18 183 1614
Fragments arranged by size:
1614 183 18
BsiEI CG_ry'CG
Cuts at: 0 130 157 167 1815
Size: 130 27 10 1648
Fragments arranged by size:
1648 130 27 10
BsiHKAI G_wGCw'C
Cuts at: 0 356 781 822 1683 1815
Size: 356 425 41 861 132
Fragments arranged by size:
861 425 356 132 41
Bsp1286I G_dGCh'C
Cuts at: 0 92 320 356 781 822 1683 1815
Size: 92 228 36 425 41 861 132
Fragments arranged by size:
861 425 228 132 92 41 36
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1317 1815
Size: 1317 498
BsrDI GCAATG_nn'
Cuts at: 0 1332 1815
Size: 1332 483
BsrFI r'CCGG_y
Cuts at: 0 162 255 402 1815
Size: 162 93 147 1413
Fragments arranged by size:
1413 162 147 93
BstAPI GCAn_nnn'nTGC
Cuts at: 0 343 1083 1815
Size: 343 740 732
Fragments arranged by size:
740 732 343
BstYI r'GATC_y
Cuts at: 0 1272 1407 1815
Size: 1272 135 408
Fragments arranged by size:
1272 408 135
BtgI C'CryG_G
Cuts at: 0 121 151 290 1276 1815
Size: 121 30 139 986 539
Fragments arranged by size:
986 539 139 121 30
DraI TTT'AAA
Cuts at: 0 1602 1815
Size: 1602 213
DraIII CAC_nnn'GTG
Cuts at: 0 826 1815
Size: 826 989
DrdI GACnn_nn'nnGTC
Cuts at: 0 889 1815
Size: 889 926
EaeI y'GGCC_r
Cuts at: 0 154 164 478 1279 1554 1815
Size: 154 10 314 801 275 261
Fragments arranged by size:
801 314 275 261 154 10
EagI C'GGCC_G
Cuts at: 0 154 164 1815
Size: 154 10 1651
Fragments arranged by size:
1651 154 10
EarI CTCTTCn'nnn_
Cuts at: 0 1610 1815
Size: 1610 205
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 487 1815
Size: 487 1328
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 125 834 1092 1112 1285 1815
Size: 125 709 258 20 173 530
Fragments arranged by size:
709 530 258 173 125 20
EcoO109I rG'GnC_Cy
Cuts at: 0 248 1815
Size: 248 1567
EcoRI G'AATT_C
Cuts at: 0 593 1290 1815
Size: 593 697 525
Fragments arranged by size:
697 593 525
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 633 1815
Size: 633 1182
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 14 46 665 697 734 766 961 993
Size: 14 32 619 32 37 32 195 32
Cuts at: 993 1364 1396 1533 1565 1815
Size: 371 32 137 32 250
Fragments arranged by size:
619 371 250 195 137 37 32 32
32 32 32 32 14
HincII GTy'rAC
Cuts at: 0 1547 1815
Size: 1547 268
MfeI C'AATT_G
Cuts at: 0 1484 1815
Size: 1484 331
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 62 1615 1815
Size: 62 1553 200
Fragments arranged by size:
1553 200 62
MscI TGG'CCA
Cuts at: 0 480 1281 1556 1815
Size: 480 801 275 259
Fragments arranged by size:
801 480 275 259
MslI CAynn'nnrTG
Cuts at: 0 550 569 979 1519 1815
Size: 550 19 410 540 296
Fragments arranged by size:
550 540 410 296 19
MspA1I CmG'CkG
Cuts at: 0 123 153 205 269 1070 1360 1815
Size: 123 30 52 64 801 290 455
Fragments arranged by size:
801 455 290 123 64 52 30
NaeI GCC'GGC
Cuts at: 0 164 257 404 1815
Size: 164 93 147 1411
Fragments arranged by size:
1411 164 147 93
NcoI C'CATG_G
Cuts at: 0 1276 1815
Size: 1276 539
NgoMIV G'CCGG_C
Cuts at: 0 162 255 402 1815
Size: 162 93 147 1413
Fragments arranged by size:
1413 162 147 93
NruI TCG'CGA
Cuts at: 0 227 1815
Size: 227 1588
PfoI T'CCnGG_A
Cuts at: 0 236 1815
Size: 236 1579
PvuII CAG'CTG
Cuts at: 0 1360 1815
Size: 1360 455
SacII CC_GC'GG
Cuts at: 0 124 154 1815
Size: 124 30 1661
Fragments arranged by size:
1661 124 30
SmaI CCC'GGG
Cuts at: 0 349 1815
Size: 349 1466
SmlI C'TyrA_G
Cuts at: 0 1370 1662 1815
Size: 1370 292 153
Fragments arranged by size:
1370 292 153
SpeI A'CTAG_T
Cuts at: 0 1102 1815
Size: 1102 713
StyI C'CwwG_G
Cuts at: 0 665 1276 1282 1383 1815
Size: 665 611 6 101 432
Fragments arranged by size:
665 611 432 101 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1006 1815
Size: 1006 809
TatI w'GTAC_w
Cuts at: 0 11 56 1815
Size: 11 45 1759
Fragments arranged by size:
1759 45 11
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 343 375 1815
Size: 343 32 1440
Fragments arranged by size:
1440 343 32
Tth111I GACn'n_nGTC
Cuts at: 0 619 1815
Size: 619 1196
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1765 1815
Size: 1765 50
XhoI C'TCGA_G
Cuts at: 0 1370 1815
Size: 1370 445
XmaI C'CCGG_G
Cuts at: 0 347 1815
Size: 347 1468
XmnI GAAnn'nnTTC
Cuts at: 0 385 1815
Size: 385 1430
Enzymes that do cut:
AcuI AflII AleI ApoI AvaI BamHI BanI BanII
BbsI BciVI BglI BlpI Bme1580I BpmI Bpu10I BsaAI
BsaHI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspMI
BsrDI BsrFI BstAPI BstYI BtgI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
Hin4I HincII MfeI MmeI MscI MslI MspA1I NaeI
NcoI NgoMIV NruI PfoI PvuII SacII SmaI SmlI
SpeI StyI TaqII TatI TstI Tth111I XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflIII AgeI
AhdI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BbeI BbvCI BcgI BclI BfrBI
BglII BmgBI BmrI BmtI BplI BpuEI BsaI BsaBI
BsaWI BsgI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I
BtgZI BtsI ClaI CspCI EcoICRI EcoNI EcoRV FseI
FspI FspAI HaeII HindIII HpaI KasI KpnI MluI
NarI NdeI NheI NotI NsiI NspI PacI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SnaBI SphI SrfI SspI StuI SwaI
XbaI ZraI