Restriction map for
pFN21AE4533
(Linear) (Six-base) MAPSORT of: KIEE4533.seq Check: 8325 from: 1 to: 1794
KIEE4533.orf
With 182 enzymes: SgfI *
March 22, 2012 18:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 281 1794
Size: 281 1513
AatII G_ACGT'C
Cuts at: 0 1409 1794
Size: 1409 385
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 773 1794
Size: 124 649 1021
Fragments arranged by size:
1021 649 124
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1709 1794
Size: 1709 85
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1435 1467 1794
Size: 1435 32 327
Fragments arranged by size:
1435 327 32
AlwNI CAG_nnn'CTG
Cuts at: 0 99 1794
Size: 99 1695
ApaI G_GGCC'C
Cuts at: 0 1157 1794
Size: 1157 637
ApoI r'AATT_y
Cuts at: 0 245 882 1794
Size: 245 637 912
Fragments arranged by size:
912 637 245
AvaI C'yCGr_G
Cuts at: 0 171 524 671 1794
Size: 171 353 147 1123
Fragments arranged by size:
1123 353 171 147
BamHI G'GATC_C
Cuts at: 0 587 1794
Size: 587 1207
BanI G'GyrC_C
Cuts at: 0 613 1315 1794
Size: 613 702 479
Fragments arranged by size:
702 613 479
BanII G_rGCy'C
Cuts at: 0 31 481 504 1157 1167 1794
Size: 31 450 23 653 10 627
Fragments arranged by size:
653 627 450 31 23 10
BbeI G_GCGC'C
Cuts at: 0 1319 1794
Size: 1319 475
BbsI GAAGACnn'nnnn_
Cuts at: 0 864 1794
Size: 864 930
BbvCI CC'TCA_GC
Cuts at: 0 494 1099 1794
Size: 494 605 695
Fragments arranged by size:
695 605 494
BciVI GTATCCnnnnn_n'
Cuts at: 0 1201 1794
Size: 1201 593
BlpI GC'TnA_GC
Cuts at: 0 415 1794
Size: 415 1379
Bme1580I G_kGCm'C
Cuts at: 0 553 985 1157 1341 1547 1688 1794
Size: 553 432 172 184 206 141 106
Fragments arranged by size:
553 432 206 184 172 141 106
BmrI ACTGGGnnnn_n'
Cuts at: 0 153 597 660 1443 1794
Size: 153 444 63 783 351
Fragments arranged by size:
783 444 351 153 63
BmtI G_CTAG'C
Cuts at: 0 1700 1794
Size: 1700 94
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 186 456 495 1794
Size: 186 270 39 1299
Fragments arranged by size:
1299 270 186 39
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 722 1200 1794
Size: 722 478 594
Fragments arranged by size:
722 594 478
Bpu10I CC'TnA_GC
Cuts at: 0 25 232 494 1099 1794
Size: 25 207 262 605 695
Fragments arranged by size:
695 605 262 207 25
BsaI GGTCTCn'nnnn_
Cuts at: 0 157 793 1794
Size: 157 636 1001
Fragments arranged by size:
1001 636 157
BsaBI GATnn'nnATC
Cuts at: 0 922 1423 1794
Size: 922 501 371
Fragments arranged by size:
922 501 371
BsaHI Gr'CG_yC
Cuts at: 0 1316 1406 1794
Size: 1316 90 388
Fragments arranged by size:
1316 388 90
BsaWI w'CCGG_w
Cuts at: 0 1204 1440 1794
Size: 1204 236 354
Fragments arranged by size:
1204 354 236
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 257 1794
Size: 257 1537
BseYI C'CCAG_C
Cuts at: 0 503 1794
Size: 503 1291
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1637 1691 1794
Size: 1637 54 103
Fragments arranged by size:
1637 103 54
BsiHKAI G_wGCw'C
Cuts at: 0 481 1167 1794
Size: 481 686 627
Fragments arranged by size:
686 627 481
Bsp1286I G_dGCh'C
Cuts at: 0 31 481 504 553 985 1157 1167 1341
Size: 31 450 23 49 432 172 10 174
Cuts at: 1341 1547 1688 1794
Size: 206 141 106
Fragments arranged by size:
450 432 206 174 172 141 106 49
31 23 10
BspEI T'CCGG_A
Cuts at: 0 1204 1794
Size: 1204 590
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 281 1794
Size: 281 1513
BsrGI T'GTAC_A
Cuts at: 0 1731 1794
Size: 1731 63
BssSI C'ACGA_G
Cuts at: 0 1634 1794
Size: 1634 160
BstBI TT'CG_AA
Cuts at: 0 255 1794
Size: 255 1539
BstXI CCAn_nnnn'nTGG
Cuts at: 0 544 610 1794
Size: 544 66 1184
Fragments arranged by size:
1184 544 66
BstYI r'GATC_y
Cuts at: 0 587 1469 1794
Size: 587 882 325
Fragments arranged by size:
882 587 325
Bsu36I CC'TnA_GG
Cuts at: 0 1766 1794
Size: 1766 28
BtgI C'CryG_G
Cuts at: 0 404 1158 1794
Size: 404 754 636
Fragments arranged by size:
754 636 404
BtsI GCAGTG_nn'
Cuts at: 0 283 1794
Size: 283 1511
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 588 623 1794
Size: 588 35 1171
Fragments arranged by size:
1171 588 35
EaeI y'GGCC_r
Cuts at: 0 1560 1794
Size: 1560 234
EarI CTCTTCn'nnn_
Cuts at: 0 464 525 809 1297 1554 1794
Size: 464 61 284 488 257 240
Fragments arranged by size:
488 464 284 257 240 61
EcoICRI GAG'CTC
Cuts at: 0 479 1165 1794
Size: 479 686 629
Fragments arranged by size:
686 629 479
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 722 773 1200 1794
Size: 124 598 51 427 594
Fragments arranged by size:
598 594 427 124 51
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1394 1794
Size: 1394 400
EcoO109I rG'GnC_Cy
Cuts at: 0 96 1153 1154 1794
Size: 96 1057 1 640
Fragments arranged by size:
1057 640 96 1
EcoRV GAT'ATC
Cuts at: 0 681 1794
Size: 681 1113
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 651 1794
Size: 651 1143
HaeII r_GCGC'y
Cuts at: 0 1319 1794
Size: 1319 475
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1272 1304 1361 1393 1794
Size: 1272 32 57 32 401
Fragments arranged by size:
1272 401 57 32 32
HincII GTy'rAC
Cuts at: 0 339 366 1065 1794
Size: 339 27 699 729
Fragments arranged by size:
729 699 339 27
HindIII A'AGCT_T
Cuts at: 0 7 1794
Size: 7 1787
HpaI GTT'AAC
Cuts at: 0 1065 1794
Size: 1065 729
KasI G'GCGC_C
Cuts at: 0 1315 1794
Size: 1315 479
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 269 834 1794
Size: 269 565 960
Fragments arranged by size:
960 565 269
MslI CAynn'nnrTG
Cuts at: 0 973 1794
Size: 973 821
MspA1I CmG'CkG
Cuts at: 0 582 1160 1174 1353 1558 1794
Size: 582 578 14 179 205 236
Fragments arranged by size:
582 578 236 205 179 14
NarI GG'CG_CC
Cuts at: 0 1316 1794
Size: 1316 478
NcoI C'CATG_G
Cuts at: 0 404 1794
Size: 404 1390
NdeI CA'TA_TG
Cuts at: 0 375 1794
Size: 375 1419
NheI G'CTAG_C
Cuts at: 0 1696 1794
Size: 1696 98
NspI r_CATG'y
Cuts at: 0 761 1794
Size: 761 1033
PasI CC'CwG_GG
Cuts at: 0 544 1087 1461 1794
Size: 544 543 374 333
Fragments arranged by size:
544 543 374 333
PflMI CCAn_nnn'nTGG
Cuts at: 0 1311 1794
Size: 1311 483
PfoI T'CCnGG_A
Cuts at: 0 461 1794
Size: 461 1333
PpuMI rG'GwC_Cy
Cuts at: 0 96 1794
Size: 96 1698
PspOMI G'GGCC_C
Cuts at: 0 1153 1794
Size: 1153 641
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 27 1794
Size: 27 1767
PstI C_TGCA'G
Cuts at: 0 278 711 1604 1794
Size: 278 433 893 190
Fragments arranged by size:
893 433 278 190
PvuII CAG'CTG
Cuts at: 0 1353 1558 1794
Size: 1353 205 236
Fragments arranged by size:
1353 236 205
SacI G_AGCT'C
Cuts at: 0 481 1167 1794
Size: 481 686 627
Fragments arranged by size:
686 627 481
SacII CC_GC'GG
Cuts at: 0 1161 1794
Size: 1161 633
SfcI C'TryA_G
Cuts at: 0 274 707 1600 1794
Size: 274 433 893 194
Fragments arranged by size:
893 433 274 194
SfoI GGC'GCC
Cuts at: 0 1317 1794
Size: 1317 477
SmaI CCC'GGG
Cuts at: 0 526 673 1794
Size: 526 147 1121
Fragments arranged by size:
1121 526 147
SphI G_CATG'C
Cuts at: 0 761 1794
Size: 761 1033
StuI AGG'CCT
Cuts at: 0 994 1039 1794
Size: 994 45 755
Fragments arranged by size:
994 755 45
StyI C'CwwG_G
Cuts at: 0 404 1794
Size: 404 1390
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 616 1794
Size: 616 1178
TatI w'GTAC_w
Cuts at: 0 1731 1794
Size: 1731 63
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1118 1150 1794
Size: 1118 32 644
Fragments arranged by size:
1118 644 32
Tth111I GACn'n_nGTC
Cuts at: 0 666 1794
Size: 666 1128
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1237 1794
Size: 1237 557
XmaI C'CCGG_G
Cuts at: 0 524 671 1794
Size: 524 147 1123
Fragments arranged by size:
1123 524 147
XmnI GAAnn'nnTTC
Cuts at: 0 818 1291 1794
Size: 818 473 503
Fragments arranged by size:
818 503 473
ZraI GAC'GTC
Cuts at: 0 1407 1794
Size: 1407 387
Enzymes that do cut:
AarI AatII AcuI AlfI AloI AlwNI ApaI ApoI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BsaI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspEI BspMI BsrGI BssSI BstBI BstXI BstYI Bsu36I
BtgI BtsI CspCI EaeI EarI EcoICRI Eco57MI EcoNI
EcoO109I EcoRV FalI HaeII Hin4I HincII HindIII HpaI
KasI MmeI MslI MspA1I NarI NcoI NdeI NheI
NspI PasI PflMI PfoI PpuMI PspOMI PsrI PstI
PvuII SacI SacII SfcI SfoI SmaI SphI StuI
StyI TaqII TatI TstI Tth111I XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI ApaLI AscI AseI AsiSI AvrII BaeI BcgI
BclI BfrBI BglI BglII BmgBI BpuEI BsaAI BsaXI
BsiEI BsiWI BsmI BsmBI BspHI BsrBI BsrDI BsrFI
BssHII BstAPI BstEII BstZ17I BtgZI ClaI DraI DraIII
DrdI EagI EciI EcoRI FseI FspI FspAI KpnI
MfeI MluI MscI NaeI NgoMIV NotI NruI NsiI
PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI
PvuI RsrII SalI SanDI SapI SbfI ScaI SexAI
SfiI SgfI SgrAI SmlI SnaBI SpeI SrfI SspI
SwaI XbaI XhoI