Restriction map for
pFN21AE4531
(Linear) (Six-base) MAPSORT of: KIEE4531.seq Check: 7381 from: 1 to: 1782
KIEE4531.orf
With 182 enzymes: SgfI *
March 22, 2012 18:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 206 1637 1782
Size: 206 1431 145
Fragments arranged by size:
1431 206 145
Acc65I G'GTAC_C
Cuts at: 0 1496 1782
Size: 1496 286
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 12 504 1782
Size: 12 492 1278
Fragments arranged by size:
1278 492 12
AleI CACnn'nnGTG
Cuts at: 0 463 1782
Size: 463 1319
AlwNI CAG_nnn'CTG
Cuts at: 0 444 972 1782
Size: 444 528 810
Fragments arranged by size:
810 528 444
ApaI G_GGCC'C
Cuts at: 0 182 452 1400 1782
Size: 182 270 948 382
Fragments arranged by size:
948 382 270 182
AvaI C'yCGr_G
Cuts at: 0 234 263 288 413 1581 1782
Size: 234 29 25 125 1168 201
Fragments arranged by size:
1168 234 201 125 29 25
BanI G'GyrC_C
Cuts at: 0 165 342 420 1496 1782
Size: 165 177 78 1076 286
Fragments arranged by size:
1076 286 177 165 78
BanII G_rGCy'C
Cuts at: 0 182 452 908 1400 1455 1588 1782
Size: 182 270 456 492 55 133 194
Fragments arranged by size:
492 456 270 194 182 133 55
BbeI G_GCGC'C
Cuts at: 0 169 346 424 1782
Size: 169 177 78 1358
Fragments arranged by size:
1358 177 169 78
BbsI GAAGACnn'nnnn_
Cuts at: 0 1152 1178 1782
Size: 1152 26 604
Fragments arranged by size:
1152 604 26
BglI GCCn_nnn'nGGC
Cuts at: 0 245 386 1306 1395 1782
Size: 245 141 920 89 387
Fragments arranged by size:
920 387 245 141 89
Bme1580I G_kGCm'C
Cuts at: 0 182 452 1400 1782
Size: 182 270 948 382
Fragments arranged by size:
948 382 270 182
BmrI ACTGGGnnnn_n'
Cuts at: 0 1104 1782
Size: 1104 678
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 626 692 1782
Size: 626 66 1090
Fragments arranged by size:
1090 626 66
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 213 637 1037 1492 1558 1741 1782
Size: 213 424 400 455 66 183 41
Fragments arranged by size:
455 424 400 213 183 66 41
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1363 1782
Size: 1363 419
BsaI GGTCTCn'nnnn_
Cuts at: 0 515 770 1782
Size: 515 255 1012
Fragments arranged by size:
1012 515 255
BsaAI yAC'GTr
Cuts at: 0 465 1242 1782
Size: 465 777 540
Fragments arranged by size:
777 540 465
BsaHI Gr'CG_yC
Cuts at: 0 166 343 421 1782
Size: 166 177 78 1361
Fragments arranged by size:
1361 177 166 78
BsaWI w'CCGG_w
Cuts at: 0 714 908 1782
Size: 714 194 874
Fragments arranged by size:
874 714 194
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 83 113 1570 1600 1782
Size: 83 30 1457 30 182
Fragments arranged by size:
1457 182 83 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1009 1040 1782
Size: 1009 31 742
Fragments arranged by size:
1009 742 31
BseYI C'CCAG_C
Cuts at: 0 307 444 1782
Size: 307 137 1338
Fragments arranged by size:
1338 307 137
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1222 1782
Size: 1222 560
BsiEI CG_ry'CG
Cuts at: 0 53 260 1782
Size: 53 207 1522
Fragments arranged by size:
1522 207 53
BsiHKAI G_wGCw'C
Cuts at: 0 908 1573 1782
Size: 908 665 209
Fragments arranged by size:
908 665 209
BsmI GAATG_Cn'
Cuts at: 0 856 968 1782
Size: 856 112 814
Fragments arranged by size:
856 814 112
Bsp1286I G_dGCh'C
Cuts at: 0 182 452 908 1400 1455 1573 1588 1782
Size: 182 270 456 492 55 118 15 194
Fragments arranged by size:
492 456 270 194 182 118 55 15
BspEI T'CCGG_A
Cuts at: 0 714 1782
Size: 714 1068
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 206 713 1637 1782
Size: 206 507 924 145
Fragments arranged by size:
924 507 206 145
BsrBI CCG'CTC
Cuts at: 0 83 270 949 1782
Size: 83 187 679 833
Fragments arranged by size:
833 679 187 83
BsrFI r'CCGG_y
Cuts at: 0 380 1305 1782
Size: 380 925 477
Fragments arranged by size:
925 477 380
BssHII G'CGCG_C
Cuts at: 0 109 111 278 302 1782
Size: 109 2 167 24 1480
Fragments arranged by size:
1480 167 109 24 2
BssSI C'ACGA_G
Cuts at: 0 1636 1782
Size: 1636 146
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1632 1782
Size: 1632 150
BstXI CCAn_nnnn'nTGG
Cuts at: 0 636 1301 1470 1782
Size: 636 665 169 312
Fragments arranged by size:
665 636 312 169
BstYI r'GATC_y
Cuts at: 0 1286 1782
Size: 1286 496
BtgI C'CryG_G
Cuts at: 0 243 689 982 1250 1421 1782
Size: 243 446 293 268 171 361
Fragments arranged by size:
446 361 293 268 243 171
BtsI GCAGTG_nn'
Cuts at: 0 336 1326 1782
Size: 336 990 456
Fragments arranged by size:
990 456 336
DraIII CAC_nnn'GTG
Cuts at: 0 210 1782
Size: 210 1572
DrdI GACnn_nn'nnGTC
Cuts at: 0 1558 1782
Size: 1558 224
EaeI y'GGCC_r
Cuts at: 0 257 314 356 692 1303 1614 1782
Size: 257 57 42 336 611 311 168
Fragments arranged by size:
611 336 311 257 168 57 42
EagI C'GGCC_G
Cuts at: 0 257 1782
Size: 257 1525
EarI CTCTTCn'nnn_
Cuts at: 0 595 986 1561 1782
Size: 595 391 575 221
Fragments arranged by size:
595 575 391 221
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 104 1782
Size: 104 1678
EcoICRI GAG'CTC
Cuts at: 0 906 1782
Size: 906 876
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 12 213 504 637 1037 1492 1558 1741
Size: 12 201 291 133 400 455 66 183
Cuts at: 1741 1782
Size: 41
Fragments arranged by size:
455 400 291 201 183 133 66 41
12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1052 1782
Size: 1052 730
EcoO109I rG'GnC_Cy
Cuts at: 0 407 448 816 1202 1396 1782
Size: 407 41 368 386 194 386
Fragments arranged by size:
407 386 386 368 194 41
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 609 1782
Size: 609 1173
FseI GG_CCGG'CC
Cuts at: 0 384 1782
Size: 384 1398
FspI TGC'GCA
Cuts at: 0 160 1782
Size: 160 1622
HaeII r_GCGC'y
Cuts at: 0 157 169 346 368 424 1782
Size: 157 12 177 22 56 1358
Fragments arranged by size:
1358 177 157 56 22 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 933 965 1035 1067 1782
Size: 933 32 70 32 715
Fragments arranged by size:
933 715 70 32 32
HincII GTy'rAC
Cuts at: 0 1275 1782
Size: 1275 507
KasI G'GCGC_C
Cuts at: 0 165 342 420 1782
Size: 165 177 78 1362
Fragments arranged by size:
1362 177 165 78
KpnI G_GTAC'C
Cuts at: 0 1500 1782
Size: 1500 282
MscI TGG'CCA
Cuts at: 0 1616 1782
Size: 1616 166
MslI CAynn'nnrTG
Cuts at: 0 463 1603 1782
Size: 463 1140 179
Fragments arranged by size:
1140 463 179
MspA1I CmG'CkG
Cuts at: 0 245 296 399 1782
Size: 245 51 103 1383
Fragments arranged by size:
1383 245 103 51
NaeI GCC'GGC
Cuts at: 0 382 1307 1782
Size: 382 925 475
Fragments arranged by size:
925 475 382
NarI GG'CG_CC
Cuts at: 0 166 343 421 1782
Size: 166 177 78 1361
Fragments arranged by size:
1361 177 166 78
NcoI C'CATG_G
Cuts at: 0 982 1782
Size: 982 800
NdeI CA'TA_TG
Cuts at: 0 804 1782
Size: 804 978
NgoMIV G'CCGG_C
Cuts at: 0 380 1305 1782
Size: 380 925 477
Fragments arranged by size:
925 477 380
NspI r_CATG'y
Cuts at: 0 750 1608 1782
Size: 750 858 174
Fragments arranged by size:
858 750 174
PflMI CCAn_nnn'nTGG
Cuts at: 0 444 1392 1782
Size: 444 948 390
Fragments arranged by size:
948 444 390
PmlI CAC'GTG
Cuts at: 0 1242 1782
Size: 1242 540
PpuMI rG'GwC_Cy
Cuts at: 0 816 1202 1782
Size: 816 386 580
Fragments arranged by size:
816 580 386
PshAI GACnn'nnGTC
Cuts at: 0 49 1782
Size: 49 1733
PspOMI G'GGCC_C
Cuts at: 0 178 448 1396 1782
Size: 178 270 948 386
Fragments arranged by size:
948 386 270 178
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 522 554 1782
Size: 522 32 1228
Fragments arranged by size:
1228 522 32
PstI C_TGCA'G
Cuts at: 0 301 1740 1782
Size: 301 1439 42
Fragments arranged by size:
1439 301 42
PvuII CAG'CTG
Cuts at: 0 296 1782
Size: 296 1486
SacI G_AGCT'C
Cuts at: 0 908 1782
Size: 908 874
SacII CC_GC'GG
Cuts at: 0 246 1782
Size: 246 1536
SapI GCTCTTCn'nnn_
Cuts at: 0 1561 1782
Size: 1561 221
ScaI AGT'ACT
Cuts at: 0 475 1782
Size: 475 1307
SexAI A'CCwGG_T
Cuts at: 0 437 1782
Size: 437 1345
SfcI C'TryA_G
Cuts at: 0 297 1215 1736 1782
Size: 297 918 521 46
Fragments arranged by size:
918 521 297 46
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 245 1782
Size: 245 1537
SfoI GGC'GCC
Cuts at: 0 167 344 422 1782
Size: 167 177 78 1360
Fragments arranged by size:
1360 177 167 78
SmaI CCC'GGG
Cuts at: 0 265 1583 1782
Size: 265 1318 199
Fragments arranged by size:
1318 265 199
SmlI C'TyrA_G
Cuts at: 0 1378 1782
Size: 1378 404
SphI G_CATG'C
Cuts at: 0 1608 1782
Size: 1608 174
SspI AAT'ATT
Cuts at: 0 922 1782
Size: 922 860
StuI AGG'CCT
Cuts at: 0 1300 1782
Size: 1300 482
StyI C'CwwG_G
Cuts at: 0 982 1336 1391 1500 1782
Size: 982 354 55 109 282
Fragments arranged by size:
982 354 282 109 55
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 39 1031 1278 1782
Size: 39 992 247 504
Fragments arranged by size:
992 504 247 39
TatI w'GTAC_w
Cuts at: 0 473 523 1782
Size: 473 50 1259
Fragments arranged by size:
1259 473 50
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 196 228 736 768 1782
Size: 196 32 508 32 1014
Fragments arranged by size:
1014 508 196 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1672 1782
Size: 1672 110
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1298 1782
Size: 1298 484
XmaI C'CCGG_G
Cuts at: 0 263 1581 1782
Size: 263 1318 201
Fragments arranged by size:
1318 263 201
Enzymes that do cut:
AarI Acc65I AcuI AleI AlwNI ApaI AvaI BanI
BanII BbeI BbsI BglI Bme1580I BmrI BplI BpmI
BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrBI
BsrFI BssHII BssSI BstAPI BstXI BstYI BtgI BtsI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I FalI FseI FspI HaeII Hin4I HincII
KasI KpnI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NspI PflMI PmlI PpuMI PshAI PspOMI
PsrI PstI PvuII SacI SacII SapI ScaI SexAI
SfcI SfiI SfoI SmaI SmlI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AhdI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BglII
BlpI BmgBI BmtI Bpu10I BsaBI BsiWI BsmBI BspHI
BsrDI BsrGI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI
CspCI DraI EcoRI EcoRV FspAI HindIII HpaI MfeI
MluI MmeI NheI NotI NruI NsiI PacI PasI
PciI PfoI PmeI PpiI PsiI PspXI PvuI RsrII
SalI SanDI SbfI SgfI SgrAI SnaBI SpeI SrfI
SwaI XbaI XhoI XmnI ZraI