Restriction map for
pFN21AE4530
(Linear) (Six-base) MAPSORT of: KIEE4530.seq Check: 4038 from: 1 to: 1776
KIEE4530.orf
With 182 enzymes: SgfI *
March 22, 2012 18:17 ..
Acc65I G'GTAC_C
Cuts at: 0 631 1776
Size: 631 1145
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 637 643 1216 1348 1391 1487 1645
Size: 103 534 6 573 132 43 96 158
Cuts at: 1645 1776
Size: 131
Fragments arranged by size:
573 534 158 132 131 103 96 43
6
AflIII A'CryG_T
Cuts at: 0 1476 1776
Size: 1476 300
AleI CACnn'nnGTG
Cuts at: 0 376 1776
Size: 376 1400
AlwNI CAG_nnn'CTG
Cuts at: 0 671 1056 1626 1776
Size: 671 385 570 150
Fragments arranged by size:
671 570 385 150
ApoI r'AATT_y
Cuts at: 0 682 781 1274 1776
Size: 682 99 493 502
Fragments arranged by size:
682 502 493 99
AvaI C'yCGr_G
Cuts at: 0 748 1776
Size: 748 1028
BanI G'GyrC_C
Cuts at: 0 631 1776
Size: 631 1145
BanII G_rGCy'C
Cuts at: 0 205 1776
Size: 205 1571
BbsI GAAGACnn'nnnn_
Cuts at: 0 1092 1776
Size: 1092 684
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 357 391 1776
Size: 357 34 1385
Fragments arranged by size:
1385 357 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 685 1355 1776
Size: 685 670 421
Fragments arranged by size:
685 670 421
Bme1580I G_kGCm'C
Cuts at: 0 251 1776
Size: 251 1525
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1033 1411 1720 1776
Size: 1033 378 309 56
Fragments arranged by size:
1033 378 309 56
Bpu10I CC'TnA_GC
Cuts at: 0 364 1221 1776
Size: 364 857 555
Fragments arranged by size:
857 555 364
BsaBI GATnn'nnATC
Cuts at: 0 462 1776
Size: 462 1314
BsaWI w'CCGG_w
Cuts at: 0 677 1776
Size: 677 1099
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1718 1748 1776
Size: 1718 30 28
Fragments arranged by size:
1718 30 28
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1578 1776
Size: 1578 198
BseYI C'CCAG_C
Cuts at: 0 743 1157 1776
Size: 743 414 619
Fragments arranged by size:
743 619 414
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 1048 1051 1478 1776
Size: 232 816 3 427 298
Fragments arranged by size:
816 427 298 232 3
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1306 1743 1776
Size: 1306 437 33
Fragments arranged by size:
1306 437 33
Bsp1286I G_dGCh'C
Cuts at: 0 205 251 1776
Size: 205 46 1525
Fragments arranged by size:
1525 205 46
BspEI T'CCGG_A
Cuts at: 0 677 1776
Size: 677 1099
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 668 1073 1776
Size: 668 405 703
Fragments arranged by size:
703 668 405
BsrGI T'GTAC_A
Cuts at: 0 380 1776
Size: 380 1396
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1524 1776
Size: 1524 252
BstBI TT'CG_AA
Cuts at: 0 1282 1776
Size: 1282 494
BstYI r'GATC_y
Cuts at: 0 11 86 341 921 1731 1776
Size: 11 75 255 580 810 45
Fragments arranged by size:
810 580 255 75 45 11
BtgI C'CryG_G
Cuts at: 0 206 1776
Size: 206 1570
BtsI GCAGTG_nn'
Cuts at: 0 921 986 1776
Size: 921 65 790
Fragments arranged by size:
921 790 65
EaeI y'GGCC_r
Cuts at: 0 353 1776
Size: 353 1423
EarI CTCTTCn'nnn_
Cuts at: 0 761 1367 1776
Size: 761 606 409
Fragments arranged by size:
761 606 409
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 637 643 1033 1216 1348 1391 1411
Size: 103 534 6 390 183 132 43 20
Cuts at: 1411 1487 1645 1720 1776
Size: 76 158 75 56
Fragments arranged by size:
534 390 183 158 132 103 76 75
56 43 20 6
EcoO109I rG'GnC_Cy
Cuts at: 0 1643 1776
Size: 1643 133
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 54 86 569 601 659 691 1204 1236
Size: 54 32 483 32 58 32 513 32
Cuts at: 1236 1554 1586 1635 1667 1716 1748 1776
Size: 318 32 49 32 49 32 28
Fragments arranged by size:
513 483 318 58 54 49 49 32
32 32 32 32 32 32 28
HincII GTy'rAC
Cuts at: 0 864 1776
Size: 864 912
HindIII A'AGCT_T
Cuts at: 0 736 1776
Size: 736 1040
KpnI G_GTAC'C
Cuts at: 0 635 1776
Size: 635 1141
MfeI C'AATT_G
Cuts at: 0 125 1776
Size: 125 1651
MslI CAynn'nnrTG
Cuts at: 0 376 1776
Size: 376 1400
MspA1I CmG'CkG
Cuts at: 0 505 976 1032 1776
Size: 505 471 56 744
Fragments arranged by size:
744 505 471 56
NspI r_CATG'y
Cuts at: 0 1766 1776
Size: 1766 10
PfoI T'CCnGG_A
Cuts at: 0 337 1049 1776
Size: 337 712 727
Fragments arranged by size:
727 712 337
PpuMI rG'GwC_Cy
Cuts at: 0 1643 1776
Size: 1643 133
PshAI GACnn'nnGTC
Cuts at: 0 898 1776
Size: 898 878
PstI C_TGCA'G
Cuts at: 0 981 1385 1776
Size: 981 404 391
Fragments arranged by size:
981 404 391
PvuII CAG'CTG
Cuts at: 0 505 976 1032 1776
Size: 505 471 56 744
Fragments arranged by size:
744 505 471 56
SapI GCTCTTCn'nnn_
Cuts at: 0 761 1776
Size: 761 1015
ScaI AGT'ACT
Cuts at: 0 1339 1776
Size: 1339 437
SfcI C'TryA_G
Cuts at: 0 977 1381 1776
Size: 977 404 395
Fragments arranged by size:
977 404 395
SmlI C'TyrA_G
Cuts at: 0 748 1776
Size: 748 1028
StyI C'CwwG_G
Cuts at: 0 953 1400 1776
Size: 953 447 376
Fragments arranged by size:
953 447 376
TatI w'GTAC_w
Cuts at: 0 380 425 1337 1776
Size: 380 45 912 439
Fragments arranged by size:
912 439 380 45
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 228 260 437 469 913 945 1776
Size: 228 32 177 32 444 32 831
Fragments arranged by size:
831 444 228 177 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1615 1776
Size: 1615 161
XbaI T'CTAG_A
Cuts at: 0 876 1251 1776
Size: 876 375 525
Fragments arranged by size:
876 525 375
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 533 1776
Size: 533 1243
XhoI C'TCGA_G
Cuts at: 0 748 1776
Size: 748 1028
XmnI GAAnn'nnTTC
Cuts at: 0 685 1278 1376 1776
Size: 685 593 98 400
Fragments arranged by size:
685 593 400 98
Enzymes that do cut:
Acc65I AcuI AflIII AleI AlwNI ApoI AvaI BanI
BanII BbsI BcgI BciVI Bme1580I BpmI Bpu10I BsaBI
BsaWI BsaXI BseRI BseYI BsgI BsmBI Bsp1286I BspEI
BspMI BsrGI BstAPI BstBI BstYI BtgI BtsI EaeI
EarI Eco57MI EcoO109I Hin4I HincII HindIII KpnI MfeI
MslI MspA1I NspI PfoI PpuMI PshAI PstI PvuII
SapI ScaI SfcI SmlI StyI TatI TstI Tth111I
XbaI XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AhdI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbvCI BclI BfrBI BglI BglII
BlpI BmgBI BmrI BmtI BplI BpuEI BsaI BsaAI
BsaHI BsiEI BsiHKAI BsiWI BsmI BspHI BsrBI BsrDI
BsrFI BssHII BssSI BstEII BstXI BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HaeII
HpaI KasI MluI MmeI MscI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PflMI PmeI PmlI PpiI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SbfI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI TaqII XmaI ZraI