Restriction map for
pFN21AE4528
(Linear) (Six-base) MAPSORT of: KIEE4528.seq Check: 975 from: 1 to: 1731
KIEE4528.orf
With 182 enzymes: SgfI *
March 22, 2012 18:17 ..
AccI GT'mk_AC
Cuts at: 0 431 1731
Size: 431 1300
Acc65I G'GTAC_C
Cuts at: 0 988 1731
Size: 988 743
AfeI AGC'GCT
Cuts at: 0 628 1731
Size: 628 1103
AflIII A'CryG_T
Cuts at: 0 434 476 1400 1731
Size: 434 42 924 331
Fragments arranged by size:
924 434 331 42
AleI CACnn'nnGTG
Cuts at: 0 475 1731
Size: 475 1256
AlwNI CAG_nnn'CTG
Cuts at: 0 339 1731
Size: 339 1392
AvaI C'yCGr_G
Cuts at: 0 770 1467 1731
Size: 770 697 264
Fragments arranged by size:
770 697 264
BamHI G'GATC_C
Cuts at: 0 564 800 1731
Size: 564 236 931
Fragments arranged by size:
931 564 236
BanI G'GyrC_C
Cuts at: 0 126 532 988 1016 1178 1184 1462 1633
Size: 126 406 456 28 162 6 278 171
Cuts at: 1633 1731
Size: 98
Fragments arranged by size:
456 406 278 171 162 126 98 28
6
BanII G_rGCy'C
Cuts at: 0 746 1731
Size: 746 985
BbeI G_GCGC'C
Cuts at: 0 536 1637 1731
Size: 536 1101 94
Fragments arranged by size:
1101 536 94
BbsI GAAGACnn'nnnn_
Cuts at: 0 441 1731
Size: 441 1290
BciVI GTATCCnnnnn_n'
Cuts at: 0 912 1731
Size: 912 819
BglI GCCn_nnn'nGGC
Cuts at: 0 667 1333 1342 1627 1731
Size: 667 666 9 285 104
Fragments arranged by size:
667 666 285 104 9
BlpI GC'TnA_GC
Cuts at: 0 1036 1114 1731
Size: 1036 78 617
Fragments arranged by size:
1036 617 78
Bme1580I G_kGCm'C
Cuts at: 0 1465 1731
Size: 1465 266
BmgBI CAC'GTC
Cuts at: 0 795 1731
Size: 795 936
BmrI ACTGGGnnnn_n'
Cuts at: 0 861 1659 1683 1731
Size: 861 798 24 48
Fragments arranged by size:
861 798 48 24
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1110 1731
Size: 1110 621
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 760 1050 1602 1731
Size: 244 516 290 552 129
Fragments arranged by size:
552 516 290 244 129
Bpu10I CC'TnA_GC
Cuts at: 0 835 1731
Size: 835 896
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 979 1731
Size: 979 752
BsaI GGTCTCn'nnnn_
Cuts at: 0 1221 1731
Size: 1221 510
BsaAI yAC'GTr
Cuts at: 0 473 477 693 1641 1731
Size: 473 4 216 948 90
Fragments arranged by size:
948 473 216 90 4
BsaHI Gr'CG_yC
Cuts at: 0 343 533 1090 1634 1731
Size: 343 190 557 544 97
Fragments arranged by size:
557 544 343 190 97
BseYI C'CCAG_C
Cuts at: 0 629 1457 1731
Size: 629 828 274
Fragments arranged by size:
828 629 274
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 715 1330 1731
Size: 715 615 401
Fragments arranged by size:
715 615 401
BsiEI CG_ry'CG
Cuts at: 0 144 1731
Size: 144 1587
BsiHKAI G_wGCw'C
Cuts at: 0 230 746 1731
Size: 230 516 985
Fragments arranged by size:
985 516 230
BsiWI C'GTAC_G
Cuts at: 0 473 1731
Size: 473 1258
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1081 1731
Size: 1081 650
Bsp1286I G_dGCh'C
Cuts at: 0 230 746 1465 1731
Size: 230 516 719 266
Fragments arranged by size:
719 516 266 230
BspHI T'CATG_A
Cuts at: 0 101 1731
Size: 101 1630
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 335 687 1731
Size: 335 352 1044
Fragments arranged by size:
1044 352 335
BsrBI CCG'CTC
Cuts at: 0 873 1586 1731
Size: 873 713 145
Fragments arranged by size:
873 713 145
BsrFI r'CCGG_y
Cuts at: 0 230 1013 1181 1472 1630 1697 1731
Size: 230 783 168 291 158 67 34
Fragments arranged by size:
783 291 230 168 158 67 34
BsrGI T'GTAC_A
Cuts at: 0 653 1160 1731
Size: 653 507 571
Fragments arranged by size:
653 571 507
BssHII G'CGCG_C
Cuts at: 0 277 369 371 1731
Size: 277 92 2 1360
Fragments arranged by size:
1360 277 92 2
BssSI C'ACGA_G
Cuts at: 0 745 1564 1731
Size: 745 819 167
Fragments arranged by size:
819 745 167
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1428 1731
Size: 1428 303
BstYI r'GATC_y
Cuts at: 0 564 800 1731
Size: 564 236 931
Fragments arranged by size:
931 564 236
BtgI C'CryG_G
Cuts at: 0 512 728 1622 1731
Size: 512 216 894 109
Fragments arranged by size:
894 512 216 109
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 57 1731
Size: 57 1674
BtsI GCAGTG_nn'
Cuts at: 0 288 587 733 1731
Size: 288 299 146 998
Fragments arranged by size:
998 299 288 146
DraIII CAC_nnn'GTG
Cuts at: 0 198 1731
Size: 198 1533
DrdI GACnn_nn'nnGTC
Cuts at: 0 823 1095 1131 1731
Size: 823 272 36 600
Fragments arranged by size:
823 600 272 36
EaeI y'GGCC_r
Cuts at: 0 1244 1325 1484 1619 1655 1731
Size: 1244 81 159 135 36 76
Fragments arranged by size:
1244 159 135 81 76 36
EarI CTCTTCn'nnn_
Cuts at: 0 217 1731
Size: 217 1514
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 291 336 574 1491 1719 1731
Size: 291 45 238 917 228 12
Fragments arranged by size:
917 291 238 228 45 12
EcoICRI GAG'CTC
Cuts at: 0 744 1731
Size: 744 987
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 760 1050 1602 1731
Size: 244 516 290 552 129
Fragments arranged by size:
552 516 290 244 129
EcoO109I rG'GnC_Cy
Cuts at: 0 176 315 417 462 765 1070 1542 1731
Size: 176 139 102 45 303 305 472 189
Fragments arranged by size:
472 305 303 189 176 139 102 45
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1161 1353 1731
Size: 1161 192 378
Fragments arranged by size:
1161 378 192
FspI TGC'GCA
Cuts at: 0 1366 1731
Size: 1366 365
HaeII r_GCGC'y
Cuts at: 0 292 536 592 630 673 1288 1435 1519
Size: 292 244 56 38 43 615 147 84
Cuts at: 1519 1528 1637 1731
Size: 9 109 94
Fragments arranged by size:
615 292 244 147 109 94 84 56
43 38 9
HincII GTy'rAC
Cuts at: 0 432 1664 1731
Size: 432 1232 67
Fragments arranged by size:
1232 432 67
KasI G'GCGC_C
Cuts at: 0 532 1633 1731
Size: 532 1101 98
Fragments arranged by size:
1101 532 98
KpnI G_GTAC'C
Cuts at: 0 992 1731
Size: 992 739
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 701 1731
Size: 701 1030
MscI TGG'CCA
Cuts at: 0 1327 1657 1731
Size: 1327 330 74
Fragments arranged by size:
1327 330 74
MslI CAynn'nnrTG
Cuts at: 0 394 475 1731
Size: 394 81 1256
Fragments arranged by size:
1256 394 81
MspA1I CmG'CkG
Cuts at: 0 41 132 261 514 914 1076 1624 1731
Size: 41 91 129 253 400 162 548 107
Fragments arranged by size:
548 400 253 162 129 107 91 41
NaeI GCC'GGC
Cuts at: 0 1474 1632 1699 1731
Size: 1474 158 67 32
Fragments arranged by size:
1474 158 67 32
NarI GG'CG_CC
Cuts at: 0 533 1634 1731
Size: 533 1101 97
Fragments arranged by size:
1101 533 97
NcoI C'CATG_G
Cuts at: 0 728 1731
Size: 728 1003
NgoMIV G'CCGG_C
Cuts at: 0 1472 1630 1697 1731
Size: 1472 158 67 34
Fragments arranged by size:
1472 158 67 34
NspI r_CATG'y
Cuts at: 0 438 1035 1404 1731
Size: 438 597 369 327
Fragments arranged by size:
597 438 369 327
PasI CC'CwG_GG
Cuts at: 0 503 1731
Size: 503 1228
PciI A'CATG_T
Cuts at: 0 434 1400 1731
Size: 434 966 331
Fragments arranged by size:
966 434 331
PflMI CCAn_nnn'nTGG
Cuts at: 0 339 1731
Size: 339 1392
PpuMI rG'GwC_Cy
Cuts at: 0 315 417 462 765 1070 1731
Size: 315 102 45 303 305 661
Fragments arranged by size:
661 315 305 303 102 45
PstI C_TGCA'G
Cuts at: 0 33 1731
Size: 33 1698
PvuI CG_AT'CG
Cuts at: 0 144 1731
Size: 144 1587
RsrII CG'GwC_CG
Cuts at: 0 1706 1731
Size: 1706 25
SacI G_AGCT'C
Cuts at: 0 746 1731
Size: 746 985
SacII CC_GC'GG
Cuts at: 0 515 1625 1731
Size: 515 1110 106
Fragments arranged by size:
1110 515 106
SalI G'TCGA_C
Cuts at: 0 430 1731
Size: 430 1301
SexAI A'CCwGG_T
Cuts at: 0 194 1731
Size: 194 1537
SfcI C'TryA_G
Cuts at: 0 29 1731
Size: 29 1702
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1333 1342 1627 1731
Size: 1333 9 285 104
Fragments arranged by size:
1333 285 104 9
SfoI GGC'GCC
Cuts at: 0 534 1635 1731
Size: 534 1101 96
Fragments arranged by size:
1101 534 96
SgrAI Cr'CCGG_yG
Cuts at: 0 230 1731
Size: 230 1501
SmaI CCC'GGG
Cuts at: 0 772 1731
Size: 772 959
SmlI C'TyrA_G
Cuts at: 0 958 1731
Size: 958 773
SphI G_CATG'C
Cuts at: 0 1035 1731
Size: 1035 696
StuI AGG'CCT
Cuts at: 0 926 964 1609 1731
Size: 926 38 645 122
Fragments arranged by size:
926 645 122 38
StyI C'CwwG_G
Cuts at: 0 728 1279 1328 1731
Size: 728 551 49 403
Fragments arranged by size:
728 551 403 49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1284 1641 1665 1731
Size: 1284 357 24 66
Fragments arranged by size:
1284 357 66 24
TatI w'GTAC_w
Cuts at: 0 653 997 1058 1160 1670 1731
Size: 653 344 61 102 510 61
Fragments arranged by size:
653 510 344 102 61 61
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 886 918 1731
Size: 886 32 813
Fragments arranged by size:
886 813 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 735 1000 1731
Size: 735 265 731
Fragments arranged by size:
735 731 265
XmaI C'CCGG_G
Cuts at: 0 770 1731
Size: 770 961
Enzymes that do cut:
AccI Acc65I AfeI AflIII AleI AlwNI AvaI BamHI
BanI BanII BbeI BbsI BciVI BglI BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BseYI BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstXI
BstYI BtgI BtgZI BtsI DraIII DrdI EaeI EarI
EciI EcoICRI Eco57MI EcoO109I FalI FspI HaeII HincII
KasI KpnI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PpuMI PstI
PvuI RsrII SacI SacII SalI SexAI SfcI SfiI
SfoI SgrAI SmaI SmlI SphI StuI StyI TaqII
TatI TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AclI AcuI AflII AgeI AhdI AlfI
AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BbvCI BcgI BclI BfrBI BglII BmtI BsaBI
BsaWI BsaXI BseRI BsmI BspEI BsrDI BstAPI BstBI
BstEII BstZ17I Bsu36I ClaI CspCI DraI EagI EcoNI
EcoRI EcoRV FseI FspAI Hin4I HindIII HpaI MfeI
MluI NdeI NheI NotI NruI NsiI PacI PfoI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PvuII SanDI SapI SbfI ScaI SgfI SnaBI SpeI
SrfI SspI SwaI Tth111I XbaI XhoI XmnI ZraI