Restriction map for
pFN21AE4527
(Linear) (Six-base) MAPSORT of: KIEE4527.seq Check: 3282 from: 1 to: 1728
KIEE4527.orf
With 182 enzymes: SgfI *
March 22, 2012 18:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1075 1728
Size: 1075 653
AccI GT'mk_AC
Cuts at: 0 89 1728
Size: 89 1639
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 135 1728
Size: 135 1593
AfeI AGC'GCT
Cuts at: 0 142 1728
Size: 142 1586
AlwNI CAG_nnn'CTG
Cuts at: 0 1090 1723 1728
Size: 1090 633 5
Fragments arranged by size:
1090 633 5
ApoI r'AATT_y
Cuts at: 0 276 807 1332 1538 1576 1728
Size: 276 531 525 206 38 152
Fragments arranged by size:
531 525 276 206 152 38
AseI AT'TA_AT
Cuts at: 0 305 1728
Size: 305 1423
AvrII C'CTAG_G
Cuts at: 0 168 1728
Size: 168 1560
BanII G_rGCy'C
Cuts at: 0 194 1728
Size: 194 1534
BglII A'GATC_T
Cuts at: 0 117 1728
Size: 117 1611
BmgBI CAC'GTC
Cuts at: 0 1323 1728
Size: 1323 405
BmrI ACTGGGnnnn_n'
Cuts at: 0 639 725 1142 1728
Size: 639 86 417 586
Fragments arranged by size:
639 586 417 86
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 1728
Size: 202 1526
Bpu10I CC'TnA_GC
Cuts at: 0 1220 1728
Size: 1220 508
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1397 1581 1728
Size: 1397 184 147
Fragments arranged by size:
1397 184 147
BsaI GGTCTCn'nnnn_
Cuts at: 0 1510 1728
Size: 1510 218
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1598 1628 1728
Size: 1598 30 100
Fragments arranged by size:
1598 100 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 278 364 729 1728
Size: 278 86 365 999
Fragments arranged by size:
999 365 278 86
BseYI C'CCAG_C
Cuts at: 0 781 910 1520 1728
Size: 781 129 610 208
Fragments arranged by size:
781 610 208 129
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 980 1728
Size: 980 748
BsiHKAI G_wGCw'C
Cuts at: 0 997 1728
Size: 997 731
BsmBI CGTCTCn'nnnn_
Cuts at: 0 7 1728
Size: 7 1721
Bsp1286I G_dGCh'C
Cuts at: 0 194 997 1728
Size: 194 803 731
Fragments arranged by size:
803 731 194
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1075 1563 1728
Size: 1075 488 165
Fragments arranged by size:
1075 488 165
BssHII G'CGCG_C
Cuts at: 0 212 214 1728
Size: 212 2 1514
Fragments arranged by size:
1514 212 2
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1090 1728
Size: 1090 638
BstYI r'GATC_y
Cuts at: 0 117 438 1728
Size: 117 321 1290
Fragments arranged by size:
1290 321 117
BtsI GCAGTG_nn'
Cuts at: 0 626 1728
Size: 626 1102
EarI CTCTTCn'nnn_
Cuts at: 0 251 394 527 1041 1170 1187 1728
Size: 251 143 133 514 129 17 541
Fragments arranged by size:
541 514 251 143 133 129 17
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 124 1728
Size: 124 1604
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 135 202 1728
Size: 135 67 1526
Fragments arranged by size:
1526 135 67
EcoO109I rG'GnC_Cy
Cuts at: 0 161 1728
Size: 161 1567
EcoRI G'AATT_C
Cuts at: 0 276 1728
Size: 276 1452
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1216 1728
Size: 1216 512
HaeII r_GCGC'y
Cuts at: 0 144 1728
Size: 144 1584
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 92 124 736 768 1438 1470 1728
Size: 92 32 612 32 670 32 258
Fragments arranged by size:
670 612 258 92 32 32 32
HincII GTy'rAC
Cuts at: 0 90 1728
Size: 90 1638
HindIII A'AGCT_T
Cuts at: 0 1438 1728
Size: 1438 290
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1398 1728
Size: 1398 330
MspA1I CmG'CkG
Cuts at: 0 1405 1728
Size: 1405 323
NspI r_CATG'y
Cuts at: 0 1236 1728
Size: 1236 492
PasI CC'CwG_GG
Cuts at: 0 1063 1728
Size: 1063 665
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 633 665 717 749 1728
Size: 633 32 52 32 979
Fragments arranged by size:
979 633 52 32 32
PshAI GACnn'nnGTC
Cuts at: 0 1382 1728
Size: 1382 346
PstI C_TGCA'G
Cuts at: 0 873 1086 1728
Size: 873 213 642
Fragments arranged by size:
873 642 213
PvuII CAG'CTG
Cuts at: 0 1405 1728
Size: 1405 323
SalI G'TCGA_C
Cuts at: 0 88 1728
Size: 88 1640
SfcI C'TryA_G
Cuts at: 0 281 869 1082 1380 1728
Size: 281 588 213 298 348
Fragments arranged by size:
588 348 298 281 213
SmlI C'TyrA_G
Cuts at: 0 1412 1560 1728
Size: 1412 148 168
Fragments arranged by size:
1412 168 148
SphI G_CATG'C
Cuts at: 0 1236 1728
Size: 1236 492
SspI AAT'ATT
Cuts at: 0 382 1499 1728
Size: 382 1117 229
Fragments arranged by size:
1117 382 229
StuI AGG'CCT
Cuts at: 0 173 1034 1728
Size: 173 861 694
Fragments arranged by size:
861 694 173
StyI C'CwwG_G
Cuts at: 0 168 1728
Size: 168 1560
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 508 1383 1728
Size: 508 875 345
Fragments arranged by size:
875 508 345
TatI w'GTAC_w
Cuts at: 0 612 1714 1728
Size: 612 1102 14
Fragments arranged by size:
1102 612 14
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 687 719 1728
Size: 687 32 1009
Fragments arranged by size:
1009 687 32
Enzymes that do cut:
AarI AccI AcuI AfeI AlwNI ApoI AseI AvrII
BanII BglII BmgBI BmrI BpmI Bpu10I BpuEI BsaI
BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI
BssHII BstAPI BstYI BtsI EarI EciI Eco57MI EcoO109I
EcoRI FalI HaeII Hin4I HincII HindIII MmeI MspA1I
NspI PasI PpiI PshAI PstI PvuII SalI SfcI
SmlI SphI SspI StuI StyI TaqII TatI TstI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI AhdI AleI
AlfI AloI ApaI ApaLI AscI AsiSI AvaI BaeI
BamHI BanI BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglI BlpI Bme1580I BmtI BplI BsaAI BsaBI
BsaHI BsaWI BsiEI BsiWI BsmI BspEI BspHI BsrBI
BsrDI BsrFI BsrGI BssSI BstBI BstEII BstXI BstZ17I
Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII DrdI
EaeI EagI EcoICRI EcoNI EcoRV FseI FspI FspAI
HpaI KasI KpnI MfeI MluI MscI MslI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PciI PflMI PfoI PmeI PmlI PpuMI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI SwaI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI