Restriction map for
pFN21AE4525
(Linear) (Six-base) MAPSORT of: KIEE4525.seq Check: 6890 from: 1 to: 1704
KIEE4525.orf
With 182 enzymes: SgfI *
March 22, 2012 18:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 288 1704
Size: 288 1416
Acc65I G'GTAC_C
Cuts at: 0 1507 1704
Size: 1507 197
AflIII A'CryG_T
Cuts at: 0 1358 1704
Size: 1358 346
AhdI GACnn_n'nnGTC
Cuts at: 0 288 1341 1704
Size: 288 1053 363
Fragments arranged by size:
1053 363 288
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 755 1704
Size: 755 949
AlwNI CAG_nnn'CTG
Cuts at: 0 141 583 1704
Size: 141 442 1121
Fragments arranged by size:
1121 442 141
ApaI G_GGCC'C
Cuts at: 0 238 1704
Size: 238 1466
ApoI r'AATT_y
Cuts at: 0 130 573 1375 1704
Size: 130 443 802 329
Fragments arranged by size:
802 443 329 130
AvaI C'yCGr_G
Cuts at: 0 231 463 1704
Size: 231 232 1241
Fragments arranged by size:
1241 232 231
BamHI G'GATC_C
Cuts at: 0 1481 1704
Size: 1481 223
BanI G'GyrC_C
Cuts at: 0 468 537 735 1507 1704
Size: 468 69 198 772 197
Fragments arranged by size:
772 468 198 197 69
BanII G_rGCy'C
Cuts at: 0 230 238 341 446 620 728 1704
Size: 230 8 103 105 174 108 976
Fragments arranged by size:
976 230 174 108 105 103 8
BbeI G_GCGC'C
Cuts at: 0 541 739 1704
Size: 541 198 965
Fragments arranged by size:
965 541 198
BbsI GAAGACnn'nnnn_
Cuts at: 0 1501 1704
Size: 1501 203
BbvCI CC'TCA_GC
Cuts at: 0 533 641 1704
Size: 533 108 1063
Fragments arranged by size:
1063 533 108
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1596 1630 1704
Size: 1596 34 74
Fragments arranged by size:
1596 74 34
BglI GCCn_nnn'nGGC
Cuts at: 0 112 673 1704
Size: 112 561 1031
Fragments arranged by size:
1031 561 112
BglII A'GATC_T
Cuts at: 0 31 1704
Size: 31 1673
Bme1580I G_kGCm'C
Cuts at: 0 238 473 1704
Size: 238 235 1231
Fragments arranged by size:
1231 238 235
BmgBI CAC'GTC
Cuts at: 0 1359 1704
Size: 1359 345
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 634 820 1318 1704
Size: 634 186 498 386
Fragments arranged by size:
634 498 386 186
Bpu10I CC'TnA_GC
Cuts at: 0 187 533 641 1243 1704
Size: 187 346 108 602 461
Fragments arranged by size:
602 461 346 187 108
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 325 712 1704
Size: 325 387 992
Fragments arranged by size:
992 387 325
BsaAI yAC'GTr
Cuts at: 0 780 1704
Size: 780 924
BsaBI GATnn'nnATC
Cuts at: 0 403 1704
Size: 403 1301
BsaHI Gr'CG_yC
Cuts at: 0 208 370 538 736 1004 1704
Size: 208 162 168 198 268 700
Fragments arranged by size:
700 268 208 198 168 162
BsaWI w'CCGG_w
Cuts at: 0 11 431 1007 1704
Size: 11 420 576 697
Fragments arranged by size:
697 576 420 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 61 958 1009 1138 1315 1690 1704
Size: 61 897 51 129 177 375 14
Fragments arranged by size:
897 375 177 129 61 51 14
BseYI C'CCAG_C
Cuts at: 0 550 555 626 682 1704
Size: 550 5 71 56 1022
Fragments arranged by size:
1022 550 71 56 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 949 1704
Size: 949 755
BsiEI CG_ry'CG
Cuts at: 0 248 1704
Size: 248 1456
BsiHKAI G_wGCw'C
Cuts at: 0 230 1704
Size: 230 1474
Bsp1286I G_dGCh'C
Cuts at: 0 230 238 341 446 473 620 728 1704
Size: 230 8 103 105 27 147 108 976
Fragments arranged by size:
976 230 147 108 105 103 27 8
BspEI T'CCGG_A
Cuts at: 0 1007 1704
Size: 1007 697
BspHI T'CATG_A
Cuts at: 0 1082 1704
Size: 1082 622
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 288 1124 1346 1559 1704
Size: 288 836 222 213 145
Fragments arranged by size:
836 288 222 213 145
BsrBI CCG'CTC
Cuts at: 0 866 1704
Size: 866 838
BsrFI r'CCGG_y
Cuts at: 0 252 378 1198 1397 1704
Size: 252 126 820 199 307
Fragments arranged by size:
820 307 252 199 126
BssSI C'ACGA_G
Cuts at: 0 1456 1704
Size: 1456 248
BstXI CCAn_nnnn'nTGG
Cuts at: 0 300 507 1671 1704
Size: 300 207 1164 33
Fragments arranged by size:
1164 300 207 33
BstYI r'GATC_y
Cuts at: 0 14 31 512 1481 1704
Size: 14 17 481 969 223
Fragments arranged by size:
969 481 223 17 14
Bsu36I CC'TnA_GG
Cuts at: 0 1210 1704
Size: 1210 494
BtgI C'CryG_G
Cuts at: 0 1658 1704
Size: 1658 46
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1257 1704
Size: 1257 447
BtsI GCAGTG_nn'
Cuts at: 0 473 1170 1455 1704
Size: 473 697 285 249
Fragments arranged by size:
697 473 285 249
DraIII CAC_nnn'GTG
Cuts at: 0 1537 1704
Size: 1537 167
DrdI GACnn_nn'nnGTC
Cuts at: 0 1165 1704
Size: 1165 539
EaeI y'GGCC_r
Cuts at: 0 113 245 701 1200 1661 1704
Size: 113 132 456 499 461 43
Fragments arranged by size:
499 461 456 132 113 43
EagI C'GGCC_G
Cuts at: 0 245 1704
Size: 245 1459
EarI CTCTTCn'nnn_
Cuts at: 0 904 1704
Size: 904 800
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1297 1704
Size: 1297 407
EcoICRI GAG'CTC
Cuts at: 0 228 1704
Size: 228 1476
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 634 820 1318 1704
Size: 634 186 498 386
Fragments arranged by size:
634 498 386 186
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1120 1246 1642 1704
Size: 1120 126 396 62
Fragments arranged by size:
1120 396 126 62
EcoO109I rG'GnC_Cy
Cuts at: 0 138 235 459 759 1214 1557 1704
Size: 138 97 224 300 455 343 147
Fragments arranged by size:
455 343 300 224 147 138 97
EcoRI G'AATT_C
Cuts at: 0 573 1704
Size: 573 1131
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 849 1704
Size: 849 855
HaeII r_GCGC'y
Cuts at: 0 541 739 1638 1658 1704
Size: 541 198 899 20 46
Fragments arranged by size:
899 541 198 46 20
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1 33 241 273 274 306 958 990
Size: 1 32 208 32 1 32 652 32
Cuts at: 990 1327 1359 1372 1404 1704
Size: 337 32 13 32 300
Fragments arranged by size:
652 337 300 208 32 32 32 32
32 32 13 1 1
HincII GTy'rAC
Cuts at: 0 1131 1257 1470 1704
Size: 1131 126 213 234
Fragments arranged by size:
1131 234 213 126
KasI G'GCGC_C
Cuts at: 0 537 735 1704
Size: 537 198 969
Fragments arranged by size:
969 537 198
KpnI G_GTAC'C
Cuts at: 0 1511 1704
Size: 1511 193
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1370 1704
Size: 1370 334
MscI TGG'CCA
Cuts at: 0 115 1663 1704
Size: 115 1548 41
Fragments arranged by size:
1548 115 41
MslI CAynn'nnrTG
Cuts at: 0 850 1357 1704
Size: 850 507 347
Fragments arranged by size:
850 507 347
MspA1I CmG'CkG
Cuts at: 0 600 686 1323 1704
Size: 600 86 637 381
Fragments arranged by size:
637 600 381 86
NarI GG'CG_CC
Cuts at: 0 538 736 1704
Size: 538 198 968
Fragments arranged by size:
968 538 198
NcoI C'CATG_G
Cuts at: 0 1658 1704
Size: 1658 46
NspI r_CATG'y
Cuts at: 0 1581 1704
Size: 1581 123
PflMI CCAn_nnn'nTGG
Cuts at: 0 141 567 1704
Size: 141 426 1137
Fragments arranged by size:
1137 426 141
PfoI T'CCnGG_A
Cuts at: 0 134 797 1154 1418 1704
Size: 134 663 357 264 286
Fragments arranged by size:
663 357 286 264 134
PpuMI rG'GwC_Cy
Cuts at: 0 138 459 1557 1704
Size: 138 321 1098 147
Fragments arranged by size:
1098 321 147 138
PspOMI G'GGCC_C
Cuts at: 0 234 1704
Size: 234 1470
PstI C_TGCA'G
Cuts at: 0 605 1121 1704
Size: 605 516 583
Fragments arranged by size:
605 583 516
PvuII CAG'CTG
Cuts at: 0 600 686 1704
Size: 600 86 1018
Fragments arranged by size:
1018 600 86
SacI G_AGCT'C
Cuts at: 0 230 1704
Size: 230 1474
SbfI CC_TGCA'GG
Cuts at: 0 1121 1704
Size: 1121 583
SexAI A'CCwGG_T
Cuts at: 0 1463 1704
Size: 1463 241
SfcI C'TryA_G
Cuts at: 0 601 1117 1704
Size: 601 516 587
Fragments arranged by size:
601 587 516
SfoI GGC'GCC
Cuts at: 0 539 737 1704
Size: 539 198 967
Fragments arranged by size:
967 539 198
SmlI C'TyrA_G
Cuts at: 0 340 727 1704
Size: 340 387 977
Fragments arranged by size:
977 387 340
StyI C'CwwG_G
Cuts at: 0 203 239 455 566 1277 1485 1658 1704
Size: 203 36 216 111 711 208 173 46
Fragments arranged by size:
711 216 208 203 173 111 46 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 75 139 1704
Size: 75 64 1565
Fragments arranged by size:
1565 75 64
Enzymes that do cut:
AarI Acc65I AflIII AhdI AlfI AlwNI ApaI ApoI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BglI BglII Bme1580I BmgBI BpmI Bpu10I BpuEI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BssSI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI HaeII Hin4I HincII KasI KpnI MmeI MscI
MslI MspA1I NarI NcoI NspI PflMI PfoI PpuMI
PspOMI PstI PvuII SacI SbfI SexAI SfcI SfoI
SmlI StyI TaqII
Enzymes that do not cut:
AatII AccI AclI AcuI AfeI AflII AgeI AleI
AloI ApaLI AscI AseI AsiSI AvrII BaeI BciVI
BclI BfrBI BlpI BmrI BmtI BplI BsaI BsaXI
BsiWI BsmI BsmBI BsrDI BsrGI BssHII BstAPI BstBI
BstEII BstZ17I ClaI CspCI DraI EcoRV FseI FspI
FspAI HindIII HpaI MfeI MluI NaeI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SacII SalI SanDI SapI ScaI SfiI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI