Restriction map for
pFN21AE4524
(Linear) (Six-base) MAPSORT of: KIEE4524.seq Check: 860 from: 1 to: 1698
KIEE4524.orf
With 182 enzymes: SgfI *
March 19, 2012 13:10 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 359 516 1016 1633 1698
Size: 359 157 500 617 65
Fragments arranged by size:
617 500 359 157 65
AflIII A'CryG_T
Cuts at: 0 1331 1698
Size: 1331 367
AleI CACnn'nnGTG
Cuts at: 0 1140 1698
Size: 1140 558
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1601 1698
Size: 1601 97
AlwNI CAG_nnn'CTG
Cuts at: 0 640 1611 1698
Size: 640 971 87
Fragments arranged by size:
971 640 87
ApaI G_GGCC'C
Cuts at: 0 1431 1698
Size: 1431 267
ApaLI G'TGCA_C
Cuts at: 0 258 1698
Size: 258 1440
ApoI r'AATT_y
Cuts at: 0 904 1017 1489 1698
Size: 904 113 472 209
Fragments arranged by size:
904 472 209 113
BamHI G'GATC_C
Cuts at: 0 413 1698
Size: 413 1285
BanI G'GyrC_C
Cuts at: 0 1447 1585 1698
Size: 1447 138 113
Fragments arranged by size:
1447 138 113
BanII G_rGCy'C
Cuts at: 0 881 1412 1431 1698
Size: 881 531 19 267
Fragments arranged by size:
881 531 267 19
BbsI GAAGACnn'nnnn_
Cuts at: 0 347 871 1129 1698
Size: 347 524 258 569
Fragments arranged by size:
569 524 347 258
BglI GCCn_nnn'nGGC
Cuts at: 0 1545 1657 1698
Size: 1545 112 41
Fragments arranged by size:
1545 112 41
BlpI GC'TnA_GC
Cuts at: 0 794 1698
Size: 794 904
Bme1580I G_kGCm'C
Cuts at: 0 262 961 1431 1698
Size: 262 699 470 267
Fragments arranged by size:
699 470 267 262
BmrI ACTGGGnnnn_n'
Cuts at: 0 573 1698
Size: 573 1125
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 212 1431 1467 1698
Size: 212 1219 36 231
Fragments arranged by size:
1219 231 212 36
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1337 1698
Size: 1337 361
Bpu10I CC'TnA_GC
Cuts at: 0 1172 1698
Size: 1172 526
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 313 1698
Size: 313 1385
BsaI GGTCTCn'nnnn_
Cuts at: 0 923 1388 1698
Size: 923 465 310
Fragments arranged by size:
923 465 310
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 662 692 1698
Size: 662 30 1006
Fragments arranged by size:
1006 662 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 683 889 1474 1698
Size: 683 206 585 224
Fragments arranged by size:
683 585 224 206
BseYI C'CCAG_C
Cuts at: 0 99 596 814 1698
Size: 99 497 218 884
Fragments arranged by size:
884 497 218 99
BsiHKAI G_wGCw'C
Cuts at: 0 262 881 1323 1412 1698
Size: 262 619 442 89 286
Fragments arranged by size:
619 442 286 262 89
BsmI GAATG_Cn'
Cuts at: 0 240 1698
Size: 240 1458
BsmBI CGTCTCn'nnnn_
Cuts at: 0 880 1698
Size: 880 818
Bsp1286I G_dGCh'C
Cuts at: 0 262 881 961 1323 1412 1431 1698
Size: 262 619 80 362 89 19 267
Fragments arranged by size:
619 362 267 262 89 80 19
BsrDI GCAATG_nn'
Cuts at: 0 611 1166 1518 1698
Size: 611 555 352 180
Fragments arranged by size:
611 555 352 180
BsrFI r'CCGG_y
Cuts at: 0 399 1698
Size: 399 1299
BstEII G'GTnAC_C
Cuts at: 0 495 568 1698
Size: 495 73 1130
Fragments arranged by size:
1130 495 73
BstXI CCAn_nnnn'nTGG
Cuts at: 0 150 1698
Size: 150 1548
BstYI r'GATC_y
Cuts at: 0 267 413 1698
Size: 267 146 1285
Fragments arranged by size:
1285 267 146
Bsu36I CC'TnA_GG
Cuts at: 0 187 1698
Size: 187 1511
BtgI C'CryG_G
Cuts at: 0 41 217 417 1398 1698
Size: 41 176 200 981 300
Fragments arranged by size:
981 300 200 176 41
BtsI GCAGTG_nn'
Cuts at: 0 809 849 1667 1698
Size: 809 40 818 31
Fragments arranged by size:
818 809 40 31
DraI TTT'AAA
Cuts at: 0 1439 1698
Size: 1439 259
DrdI GACnn_nn'nnGTC
Cuts at: 0 1131 1225 1698
Size: 1131 94 473
Fragments arranged by size:
1131 473 94
EaeI y'GGCC_r
Cuts at: 0 23 140 704 1546 1698
Size: 23 117 564 842 152
Fragments arranged by size:
842 564 152 117 23
EarI CTCTTCn'nnn_
Cuts at: 0 721 866 992 1417 1451 1698
Size: 721 145 126 425 34 247
Fragments arranged by size:
721 425 247 145 126 34
EcoICRI GAG'CTC
Cuts at: 0 879 1410 1698
Size: 879 531 288
Fragments arranged by size:
879 531 288
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 359 516 1016 1337 1633 1698
Size: 359 157 500 321 296 65
Fragments arranged by size:
500 359 321 296 157 65
EcoNI CCTnn'n_nnAGG
Cuts at: 0 577 1698
Size: 577 1121
EcoO109I rG'GnC_Cy
Cuts at: 0 1427 1428 1698
Size: 1427 1 270
Fragments arranged by size:
1427 270 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 364 732 1698
Size: 364 368 966
Fragments arranged by size:
966 368 364
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 662 694 1077 1109 1698
Size: 662 32 383 32 589
Fragments arranged by size:
662 589 383 32 32
HincII GTy'rAC
Cuts at: 0 946 1698
Size: 946 752
HindIII A'AGCT_T
Cuts at: 0 331 1356 1698
Size: 331 1025 342
Fragments arranged by size:
1025 342 331
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1023 1698
Size: 1023 675
MscI TGG'CCA
Cuts at: 0 25 142 706 1548 1698
Size: 25 117 564 842 150
Fragments arranged by size:
842 564 150 117 25
MslI CAynn'nnrTG
Cuts at: 0 1140 1310 1330 1403 1698
Size: 1140 170 20 73 295
Fragments arranged by size:
1140 295 170 73 20
MspA1I CmG'CkG
Cuts at: 0 43 664 1698
Size: 43 621 1034
Fragments arranged by size:
1034 621 43
NcoI C'CATG_G
Cuts at: 0 217 1698
Size: 217 1481
NspI r_CATG'y
Cuts at: 0 1335 1698
Size: 1335 363
PciI A'CATG_T
Cuts at: 0 1331 1698
Size: 1331 367
PfoI T'CCnGG_A
Cuts at: 0 602 1247 1698
Size: 602 645 451
Fragments arranged by size:
645 602 451
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 622 654 1698
Size: 622 32 1044
Fragments arranged by size:
1044 622 32
PspOMI G'GGCC_C
Cuts at: 0 1427 1698
Size: 1427 271
PstI C_TGCA'G
Cuts at: 0 213 1580 1698
Size: 213 1367 118
Fragments arranged by size:
1367 213 118
SacI G_AGCT'C
Cuts at: 0 881 1412 1698
Size: 881 531 286
Fragments arranged by size:
881 531 286
SacII CC_GC'GG
Cuts at: 0 44 1698
Size: 44 1654
SapI GCTCTTCn'nnn_
Cuts at: 0 992 1417 1698
Size: 992 425 281
Fragments arranged by size:
992 425 281
SexAI A'CCwGG_T
Cuts at: 0 578 1698
Size: 578 1120
SfcI C'TryA_G
Cuts at: 0 209 1576 1698
Size: 209 1367 122
Fragments arranged by size:
1367 209 122
SmlI C'TyrA_G
Cuts at: 0 328 1698
Size: 328 1370
SspI AAT'ATT
Cuts at: 0 360 1698
Size: 360 1338
StyI C'CwwG_G
Cuts at: 0 217 272 788 1698
Size: 217 55 516 910
Fragments arranged by size:
910 516 217 55
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 277 427 806 1338 1609 1698
Size: 277 150 379 532 271 89
Fragments arranged by size:
532 379 277 271 150 89
TatI w'GTAC_w
Cuts at: 0 1553 1698
Size: 1553 145
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 252 284 1698
Size: 252 32 1414
Fragments arranged by size:
1414 252 32
Tth111I GACn'n_nGTC
Cuts at: 0 1225 1698
Size: 1225 473
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1312 1659 1698
Size: 1312 347 39
Fragments arranged by size:
1312 347 39
XmnI GAAnn'nnTTC
Cuts at: 0 715 1001 1181 1698
Size: 715 286 180 517
Fragments arranged by size:
715 517 286 180
Enzymes that do cut:
AcuI AflIII AleI AlfI AlwNI ApaI ApaLI ApoI
BamHI BanI BanII BbsI BglI BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaXI BseRI BseYI
BsiHKAI BsmI BsmBI Bsp1286I BsrDI BsrFI BstEII BstXI
BstYI Bsu36I BtgI BtsI DraI DrdI EaeI EarI
EcoICRI Eco57MI EcoNI EcoO109I FalI Hin4I HincII HindIII
MmeI MscI MslI MspA1I NcoI NspI PciI PfoI
PpiI PspOMI PstI SacI SacII SapI SexAI SfcI
SmlI SspI StyI TaqII TatI TstI Tth111I XcmI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AloI AscI AseI AsiSI AvaI AvrII BaeI
BbeI BbvCI BcgI BciVI BclI BfrBI BglII BmgBI
BmtI BsaAI BsaBI BsaHI BsaWI BsgI BsiEI BsiWI
BspEI BspHI BspMI BsrBI BsrGI BssHII BssSI BstAPI
BstBI BstZ17I BtgZI ClaI CspCI DraIII EagI EciI
EcoRI EcoRV FseI FspI FspAI HaeII HpaI KasI
KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PflMI PmeI PmlI
PpuMI PshAI PsiI PspXI PsrI PvuI PvuII RsrII
SalI SanDI SbfI ScaI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI StuI SwaI XbaI
XhoI XmaI ZraI