Restriction map for
pFN21AE4522
(Linear) (Six-base) MAPSORT of: KIEE4522.seq Check: 5209 from: 1 to: 1686
KIEE4522.orf
With 182 enzymes: SgfI *
March 22, 2012 18:16 ..
AccI GT'mk_AC
Cuts at: 0 494 1686
Size: 494 1192
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 673 902 936 1686
Size: 673 229 34 750
Fragments arranged by size:
750 673 229 34
AgeI A'CCGG_T
Cuts at: 0 1141 1686
Size: 1141 545
AhdI GACnn_n'nnGTC
Cuts at: 0 347 1686
Size: 347 1339
AleI CACnn'nnGTG
Cuts at: 0 169 1157 1686
Size: 169 988 529
Fragments arranged by size:
988 529 169
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 765 1686
Size: 765 921
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 175 207 523 555 1686
Size: 175 32 316 32 1131
Fragments arranged by size:
1131 316 175 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 925 1686
Size: 925 761
ApaI G_GGCC'C
Cuts at: 0 367 1686
Size: 367 1319
ApaLI G'TGCA_C
Cuts at: 0 617 1686
Size: 617 1069
ApoI r'AATT_y
Cuts at: 0 388 437 1089 1266 1686
Size: 388 49 652 177 420
Fragments arranged by size:
652 420 388 177 49
BanI G'GyrC_C
Cuts at: 0 724 1686
Size: 724 962
BanII G_rGCy'C
Cuts at: 0 367 1686
Size: 367 1319
BbsI GAAGACnn'nnnn_
Cuts at: 0 1494 1686
Size: 1494 192
BbvCI CC'TCA_GC
Cuts at: 0 1304 1686
Size: 1304 382
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 45 79 1686
Size: 45 34 1607
Fragments arranged by size:
1607 45 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 306 1686
Size: 306 1380
BfrBI ATG'CAT
Cuts at: 0 675 1686
Size: 675 1011
BglII A'GATC_T
Cuts at: 0 656 1686
Size: 656 1030
Bme1580I G_kGCm'C
Cuts at: 0 55 367 621 729 1686
Size: 55 312 254 108 957
Fragments arranged by size:
957 312 254 108 55
BmrI ACTGGGnnnn_n'
Cuts at: 0 986 1429 1529 1686
Size: 986 443 100 157
Fragments arranged by size:
986 443 157 100
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1034 1686
Size: 1034 652
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 401 781 1686
Size: 401 380 905
Fragments arranged by size:
905 401 380
Bpu10I CC'TnA_GC
Cuts at: 0 1304 1686
Size: 1304 382
BsaI GGTCTCn'nnnn_
Cuts at: 0 814 1054 1686
Size: 814 240 632
Fragments arranged by size:
814 632 240
BsaBI GATnn'nnATC
Cuts at: 0 872 1686
Size: 872 814
BsaWI w'CCGG_w
Cuts at: 0 1141 1686
Size: 1141 545
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1141 1306 1686
Size: 1141 165 380
Fragments arranged by size:
1141 380 165
BseYI C'CCAG_C
Cuts at: 0 1640 1686
Size: 1640 46
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1652 1686
Size: 1652 34
BsiEI CG_ry'CG
Cuts at: 0 189 252 1686
Size: 189 63 1434
Fragments arranged by size:
1434 189 63
BsiHKAI G_wGCw'C
Cuts at: 0 621 1020 1686
Size: 621 399 666
Fragments arranged by size:
666 621 399
BsmI GAATG_Cn'
Cuts at: 0 956 1686
Size: 956 730
Bsp1286I G_dGCh'C
Cuts at: 0 55 367 621 729 1020 1686
Size: 55 312 254 108 291 666
Fragments arranged by size:
666 312 291 254 108 55
BsrBI CCG'CTC
Cuts at: 0 59 1514 1686
Size: 59 1455 172
Fragments arranged by size:
1455 172 59
BsrDI GCAATG_nn'
Cuts at: 0 935 1622 1686
Size: 935 687 64
Fragments arranged by size:
935 687 64
BsrFI r'CCGG_y
Cuts at: 0 144 188 1141 1686
Size: 144 44 953 545
Fragments arranged by size:
953 545 144 44
BsrGI T'GTAC_A
Cuts at: 0 1547 1686
Size: 1547 139
BssSI C'ACGA_G
Cuts at: 0 54 1008 1686
Size: 54 954 678
Fragments arranged by size:
954 678 54
BstXI CCAn_nnnn'nTGG
Cuts at: 0 171 976 1073 1686
Size: 171 805 97 613
Fragments arranged by size:
805 613 171 97
BstYI r'GATC_y
Cuts at: 0 656 704 1686
Size: 656 48 982
Fragments arranged by size:
982 656 48
BtgI C'CryG_G
Cuts at: 0 137 984 1686
Size: 137 847 702
Fragments arranged by size:
847 702 137
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 204 213 1686
Size: 204 9 1473
Fragments arranged by size:
1473 204 9
BtsI GCAGTG_nn'
Cuts at: 0 1664 1686
Size: 1664 22
ClaI AT'CG_AT
Cuts at: 0 1553 1686
Size: 1553 133
DrdI GACnn_nn'nnGTC
Cuts at: 0 828 1597 1686
Size: 828 769 89
Fragments arranged by size:
828 769 89
EaeI y'GGCC_r
Cuts at: 0 186 1260 1686
Size: 186 1074 426
Fragments arranged by size:
1074 426 186
EagI C'GGCC_G
Cuts at: 0 186 1686
Size: 186 1500
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 401 673 781 902 936 1686
Size: 401 272 108 121 34 750
Fragments arranged by size:
750 401 272 121 108 34
EcoO109I rG'GnC_Cy
Cuts at: 0 363 364 409 1338 1590 1686
Size: 363 1 45 929 252 96
Fragments arranged by size:
929 363 252 96 45 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 514 649 1686
Size: 514 135 1037
Fragments arranged by size:
1037 514 135
HaeII r_GCGC'y
Cuts at: 0 98 1686
Size: 98 1588
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 333 365 641 673 1335 1367 1686
Size: 333 32 276 32 662 32 319
Fragments arranged by size:
662 333 319 276 32 32 32
HincII GTy'rAC
Cuts at: 0 747 1686
Size: 747 939
HindIII A'AGCT_T
Cuts at: 0 578 1686
Size: 578 1108
HpaI GTT'AAC
Cuts at: 0 747 1686
Size: 747 939
MfeI C'AATT_G
Cuts at: 0 458 1686
Size: 458 1228
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1530 1686
Size: 1530 156
MslI CAynn'nnrTG
Cuts at: 0 169 1157 1686
Size: 169 988 529
Fragments arranged by size:
988 529 169
MspA1I CmG'CkG
Cuts at: 0 153 808 986 1644 1686
Size: 153 655 178 658 42
Fragments arranged by size:
658 655 178 153 42
NaeI GCC'GGC
Cuts at: 0 146 1686
Size: 146 1540
NgoMIV G'CCGG_C
Cuts at: 0 144 1686
Size: 144 1542
NsiI A_TGCA'T
Cuts at: 0 677 1686
Size: 677 1009
NspI r_CATG'y
Cuts at: 0 1005 1686
Size: 1005 681
PasI CC'CwG_GG
Cuts at: 0 358 1686
Size: 358 1328
PflMI CCAn_nnn'nTGG
Cuts at: 0 1441 1686
Size: 1441 245
PpuMI rG'GwC_Cy
Cuts at: 0 409 1590 1686
Size: 409 1181 96
Fragments arranged by size:
1181 409 96
PshAI GACnn'nnGTC
Cuts at: 0 979 1686
Size: 979 707
PspOMI G'GGCC_C
Cuts at: 0 363 1686
Size: 363 1323
PstI C_TGCA'G
Cuts at: 0 1649 1686
Size: 1649 37
PvuII CAG'CTG
Cuts at: 0 808 1644 1686
Size: 808 836 42
Fragments arranged by size:
836 808 42
SacII CC_GC'GG
Cuts at: 0 987 1686
Size: 987 699
SexAI A'CCwGG_T
Cuts at: 0 812 1276 1686
Size: 812 464 410
Fragments arranged by size:
812 464 410
SfcI C'TryA_G
Cuts at: 0 1645 1686
Size: 1645 41
SphI G_CATG'C
Cuts at: 0 1005 1686
Size: 1005 681
SspI AAT'ATT
Cuts at: 0 396 1686
Size: 396 1290
StyI C'CwwG_G
Cuts at: 0 498 1686
Size: 498 1188
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 238 1686
Size: 238 1448
TatI w'GTAC_w
Cuts at: 0 1547 1686
Size: 1547 139
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 171 1686
Size: 171 1515
XmnI GAAnn'nnTTC
Cuts at: 0 82 1270 1372 1449 1686
Size: 82 1188 102 77 237
Fragments arranged by size:
1188 237 102 82 77
Enzymes that do cut:
AccI AcuI AgeI AhdI AleI AlfI AloI AlwNI
ApaI ApaLI ApoI BanI BanII BbsI BbvCI BcgI
BciVI BfrBI BglII Bme1580I BmrI BplI BpmI Bpu10I
BsaI BsaBI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BsrBI BsrDI BsrFI BsrGI BssSI BstXI
BstYI BtgI BtgZI BtsI ClaI DrdI EaeI EagI
Eco57MI EcoO109I FalI HaeII Hin4I HincII HindIII HpaI
MfeI MmeI MslI MspA1I NaeI NgoMIV NsiI NspI
PasI PflMI PpuMI PshAI PspOMI PstI PvuII SacII
SexAI SfcI SphI SspI StyI TaqII TatI XcmI
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AscI
AseI AsiSI AvaI AvrII BaeI BamHI BbeI BclI
BglI BlpI BmgBI BmtI BpuEI BsaAI BsaHI BsaXI
BsiWI BsmBI BspEI BspHI BspMI BssHII BstAPI BstBI
BstEII BstZ17I Bsu36I CspCI DraI DraIII EarI EciI
EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI KasI
KpnI MluI MscI NarI NcoI NdeI NheI NotI
NruI PacI PciI PfoI PmeI PmlI PpiI PsiI
PspXI PsrI PvuI RsrII SacI SalI SanDI SapI
SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SrfI StuI SwaI TstI Tth111I XbaI
XhoI XmaI ZraI