Restriction map for
pFN21AE4520
(Linear) (Six-base) MAPSORT of: KIEE4520.seq Check: 8096 from: 1 to: 1671
KIEE4520.orf
With 182 enzymes: SgfI *
March 22, 2012 18:15 ..
AflII C'TTAA_G
Cuts at: 0 1662 1671
Size: 1662 9
AflIII A'CryG_T
Cuts at: 0 1121 1328 1578 1647 1671
Size: 1121 207 250 69 24
Fragments arranged by size:
1121 250 207 69 24
AleI CACnn'nnGTG
Cuts at: 0 1141 1579 1671
Size: 1141 438 92
Fragments arranged by size:
1141 438 92
ApoI r'AATT_y
Cuts at: 0 673 949 974 1528 1537 1671
Size: 673 276 25 554 9 134
Fragments arranged by size:
673 554 276 134 25 9
AseI AT'TA_AT
Cuts at: 0 421 1589 1671
Size: 421 1168 82
Fragments arranged by size:
1168 421 82
BamHI G'GATC_C
Cuts at: 0 1423 1671
Size: 1423 248
BanI G'GyrC_C
Cuts at: 0 1560 1671
Size: 1560 111
BbsI GAAGACnn'nnnn_
Cuts at: 0 331 593 1671
Size: 331 262 1078
Fragments arranged by size:
1078 331 262
BclI T'GATC_A
Cuts at: 0 314 1671
Size: 314 1357
BfrBI ATG'CAT
Cuts at: 0 779 1671
Size: 779 892
BglII A'GATC_T
Cuts at: 0 226 1671
Size: 226 1445
BlpI GC'TnA_GC
Cuts at: 0 758 1671
Size: 758 913
BmgBI CAC'GTC
Cuts at: 0 1329 1671
Size: 1329 342
BmrI ACTGGGnnnn_n'
Cuts at: 0 1518 1671
Size: 1518 153
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 436 1671
Size: 436 1235
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 450 671 1481 1671
Size: 450 221 810 190
Fragments arranged by size:
810 450 221 190
BsaWI w'CCGG_w
Cuts at: 0 815 1671
Size: 815 856
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 180 210 1279 1309 1671
Size: 180 30 1069 30 362
Fragments arranged by size:
1069 362 180 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 633 1377 1671
Size: 633 744 294
Fragments arranged by size:
744 633 294
BseYI C'CCAG_C
Cuts at: 0 899 1214 1671
Size: 899 315 457
Fragments arranged by size:
899 457 315
BsmI GAATG_Cn'
Cuts at: 0 38 174 1671
Size: 38 136 1497
Fragments arranged by size:
1497 136 38
BspEI T'CCGG_A
Cuts at: 0 815 1671
Size: 815 856
BspHI T'CATG_A
Cuts at: 0 1370 1671
Size: 1370 301
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 701 1671
Size: 701 970
BsrGI T'GTAC_A
Cuts at: 0 1331 1671
Size: 1331 340
BstXI CCAn_nnnn'nTGG
Cuts at: 0 907 1671
Size: 907 764
BstYI r'GATC_y
Cuts at: 0 175 226 1423 1671
Size: 175 51 1197 248
Fragments arranged by size:
1197 248 175 51
Bsu36I CC'TnA_GG
Cuts at: 0 508 1671
Size: 508 1163
BtgI C'CryG_G
Cuts at: 0 1427 1671
Size: 1427 244
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 257 738 1400 1671
Size: 257 481 662 271
Fragments arranged by size:
662 481 271 257
BtsI GCAGTG_nn'
Cuts at: 0 1307 1671
Size: 1307 364
EaeI y'GGCC_r
Cuts at: 0 564 1671
Size: 564 1107
EarI CTCTTCn'nnn_
Cuts at: 0 1448 1671
Size: 1448 223
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 436 1671
Size: 436 1235
EcoO109I rG'GnC_Cy
Cuts at: 0 604 1671
Size: 604 1067
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 74 416 1671
Size: 74 342 1255
Fragments arranged by size:
1255 342 74
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 193 225 1671
Size: 193 32 1446
Fragments arranged by size:
1446 193 32
HincII GTy'rAC
Cuts at: 0 1659 1671
Size: 1659 12
MluI A'CGCG_T
Cuts at: 0 1121 1671
Size: 1121 550
MscI TGG'CCA
Cuts at: 0 566 1671
Size: 566 1105
MslI CAynn'nnrTG
Cuts at: 0 572 1141 1579 1671
Size: 572 569 438 92
Fragments arranged by size:
572 569 438 92
MspA1I CmG'CkG
Cuts at: 0 903 1671
Size: 903 768
NcoI C'CATG_G
Cuts at: 0 1427 1671
Size: 1427 244
NruI TCG'CGA
Cuts at: 0 1419 1671
Size: 1419 252
NsiI A_TGCA'T
Cuts at: 0 781 1671
Size: 781 890
NspI r_CATG'y
Cuts at: 0 1582 1651 1671
Size: 1582 69 20
Fragments arranged by size:
1582 69 20
PciI A'CATG_T
Cuts at: 0 1578 1647 1671
Size: 1578 69 24
Fragments arranged by size:
1578 69 24
PfoI T'CCnGG_A
Cuts at: 0 962 1394 1671
Size: 962 432 277
Fragments arranged by size:
962 432 277
PshAI GACnn'nnGTC
Cuts at: 0 414 1671
Size: 414 1257
PstI C_TGCA'G
Cuts at: 0 281 1671
Size: 281 1390
PvuII CAG'CTG
Cuts at: 0 903 1671
Size: 903 768
SfcI C'TryA_G
Cuts at: 0 277 848 863 1671
Size: 277 571 15 808
Fragments arranged by size:
808 571 277 15
SmlI C'TyrA_G
Cuts at: 0 429 686 1496 1662 1671
Size: 429 257 810 166 9
Fragments arranged by size:
810 429 257 166 9
StuI AGG'CCT
Cuts at: 0 482 1671
Size: 482 1189
StyI C'CwwG_G
Cuts at: 0 1427 1671
Size: 1427 244
TatI w'GTAC_w
Cuts at: 0 136 1331 1671
Size: 136 1195 340
Fragments arranged by size:
1195 340 136
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 180 212 1415 1447 1671
Size: 180 32 1203 32 224
Fragments arranged by size:
1203 224 180 32 32
XbaI T'CTAG_A
Cuts at: 0 348 1152 1671
Size: 348 804 519
Fragments arranged by size:
804 519 348
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 907 1671
Size: 907 764
XmnI GAAnn'nnTTC
Cuts at: 0 652 1309 1671
Size: 652 657 362
Fragments arranged by size:
657 652 362
Enzymes that do cut:
AflII AflIII AleI ApoI AseI BamHI BanI BbsI
BclI BfrBI BglII BlpI BmgBI BmrI BpmI BpuEI
BsaWI BsaXI BseRI BseYI BsmI BspEI BspHI BspMI
BsrGI BstXI BstYI Bsu36I BtgI BtgZI BtsI EaeI
EarI Eco57MI EcoO109I FalI Hin4I HincII MluI MscI
MslI MspA1I NcoI NruI NsiI NspI PciI PfoI
PshAI PstI PvuII SfcI SmlI StuI StyI TatI
TstI XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AgeI
AhdI AlfI AloI AlwNI ApaI ApaLI AscI AsiSI
AvaI AvrII BaeI BanII BbeI BbvCI BcgI BciVI
BglI Bme1580I BmtI BplI Bpu10I BsaI BsaAI BsaBI
BsaHI BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstZ17I
ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI
EcoNI EcoRI EcoRV FseI FspI FspAI HaeII HindIII
HpaI KasI KpnI MfeI MmeI NaeI NarI NdeI
NgoMIV NheI NotI PacI PasI PflMI PmeI PmlI
PpiI PpuMI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI SwaI TaqII Tth111I XhoI XmaI ZraI