Restriction map for
pFN21AE4518
(Linear) (Six-base) MAPSORT of: KIEE4518.seq Check: 214 from: 1 to: 1656
KIEE4518.orf
With 182 enzymes: SgfI *
March 22, 2012 18:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 243 1006 1656
Size: 243 763 650
Fragments arranged by size:
763 650 243
AatII G_ACGT'C
Cuts at: 0 215 1656
Size: 215 1441
AccI GT'mk_AC
Cuts at: 0 200 1656
Size: 200 1456
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 484 1656
Size: 256 228 1172
Fragments arranged by size:
1172 256 228
AflII C'TTAA_G
Cuts at: 0 121 1656
Size: 121 1535
AflIII A'CryG_T
Cuts at: 0 161 275 1656
Size: 161 114 1381
Fragments arranged by size:
1381 161 114
AhdI GACnn_n'nnGTC
Cuts at: 0 330 1656
Size: 330 1326
AlwNI CAG_nnn'CTG
Cuts at: 0 998 1656
Size: 998 658
ApaI G_GGCC'C
Cuts at: 0 272 1656
Size: 272 1384
ApoI r'AATT_y
Cuts at: 0 980 1656
Size: 980 676
AvaI C'yCGr_G
Cuts at: 0 340 1424 1656
Size: 340 1084 232
Fragments arranged by size:
1084 340 232
BamHI G'GATC_C
Cuts at: 0 689 1656
Size: 689 967
BanI G'GyrC_C
Cuts at: 0 93 1656
Size: 93 1563
BanII G_rGCy'C
Cuts at: 0 272 632 653 1504 1656
Size: 272 360 21 851 152
Fragments arranged by size:
851 360 272 152 21
BclI T'GATC_A
Cuts at: 0 758 1656
Size: 758 898
BlpI GC'TnA_GC
Cuts at: 0 626 718 1168 1656
Size: 626 92 450 488
Fragments arranged by size:
626 488 450 92
Bme1580I G_kGCm'C
Cuts at: 0 272 1014 1648 1656
Size: 272 742 634 8
Fragments arranged by size:
742 634 272 8
BmrI ACTGGGnnnn_n'
Cuts at: 0 137 1217 1326 1656
Size: 137 1080 109 330
Fragments arranged by size:
1080 330 137 109
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1119 1523 1656
Size: 1119 404 133
Fragments arranged by size:
1119 404 133
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 835 991 1493 1518 1656
Size: 835 156 502 25 138
Fragments arranged by size:
835 502 156 138 25
BsaAI yAC'GTr
Cuts at: 0 276 1656
Size: 276 1380
BsaBI GATnn'nnATC
Cuts at: 0 178 1656
Size: 178 1478
BsaHI Gr'CG_yC
Cuts at: 0 44 212 1656
Size: 44 168 1444
Fragments arranged by size:
1444 168 44
BsaWI w'CCGG_w
Cuts at: 0 1391 1656
Size: 1391 265
BseYI C'CCAG_C
Cuts at: 0 23 39 69 223 1350 1451 1553 1656
Size: 23 16 30 154 1127 101 102 103
Fragments arranged by size:
1127 154 103 102 101 30 23 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1066 1656
Size: 1066 590
BsiHKAI G_wGCw'C
Cuts at: 0 632 1656
Size: 632 1024
BsmI GAATG_Cn'
Cuts at: 0 484 738 1656
Size: 484 254 918
Fragments arranged by size:
918 484 254
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1531 1656
Size: 1531 125
Bsp1286I G_dGCh'C
Cuts at: 0 272 632 653 1014 1504 1648 1656
Size: 272 360 21 361 490 144 8
Fragments arranged by size:
490 361 360 272 144 21 8
BspEI T'CCGG_A
Cuts at: 0 1391 1656
Size: 1391 265
BspHI T'CATG_A
Cuts at: 0 80 215 473 512 794 1656
Size: 80 135 258 39 282 862
Fragments arranged by size:
862 282 258 135 80 39
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 105 243 992 1006 1656
Size: 105 138 749 14 650
Fragments arranged by size:
749 650 138 105 14
BsrBI CCG'CTC
Cuts at: 0 1256 1544 1656
Size: 1256 288 112
Fragments arranged by size:
1256 288 112
BsrGI T'GTAC_A
Cuts at: 0 278 857 1656
Size: 278 579 799
Fragments arranged by size:
799 579 278
BssHII G'CGCG_C
Cuts at: 0 19 1656
Size: 19 1637
BssSI C'ACGA_G
Cuts at: 0 697 1656
Size: 697 959
BstXI CCAn_nnnn'nTGG
Cuts at: 0 417 1656
Size: 417 1239
BstYI r'GATC_y
Cuts at: 0 173 230 689 1656
Size: 173 57 459 967
Fragments arranged by size:
967 459 173 57
BtgI C'CryG_G
Cuts at: 0 378 1262 1656
Size: 378 884 394
Fragments arranged by size:
884 394 378
BtsI GCAGTG_nn'
Cuts at: 0 1548 1656
Size: 1548 108
DraIII CAC_nnn'GTG
Cuts at: 0 699 1656
Size: 699 957
DrdI GACnn_nn'nnGTC
Cuts at: 0 400 804 1656
Size: 400 404 852
Fragments arranged by size:
852 404 400
EaeI y'GGCC_r
Cuts at: 0 784 1410 1483 1656
Size: 784 626 73 173
Fragments arranged by size:
784 626 173 73
EarI CTCTTCn'nnn_
Cuts at: 0 658 1283 1656
Size: 658 625 373
Fragments arranged by size:
658 625 373
EcoICRI GAG'CTC
Cuts at: 0 630 1656
Size: 630 1026
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 484 835 991 1493 1518 1656
Size: 256 228 351 156 502 25 138
Fragments arranged by size:
502 351 256 228 156 138 25
EcoO109I rG'GnC_Cy
Cuts at: 0 117 269 552 1463 1627 1656
Size: 117 152 283 911 164 29
Fragments arranged by size:
911 283 164 152 117 29
EcoRI G'AATT_C
Cuts at: 0 980 1656
Size: 980 676
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 747 1656
Size: 747 909
FspI TGC'GCA
Cuts at: 0 133 1564 1656
Size: 133 1431 92
Fragments arranged by size:
1431 133 92
FspAI rTGC'GCAy
Cuts at: 0 133 1656
Size: 133 1523
HaeII r_GCGC'y
Cuts at: 0 40 77 1351 1656
Size: 40 37 1274 305
Fragments arranged by size:
1274 305 40 37
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 316 348 1656
Size: 316 32 1308
Fragments arranged by size:
1308 316 32
HincII GTy'rAC
Cuts at: 0 729 1656
Size: 729 927
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 665 1656
Size: 665 991
MscI TGG'CCA
Cuts at: 0 1412 1485 1656
Size: 1412 73 171
Fragments arranged by size:
1412 171 73
MslI CAynn'nnrTG
Cuts at: 0 85 403 1656
Size: 85 318 1253
Fragments arranged by size:
1253 318 85
MspA1I CmG'CkG
Cuts at: 0 1167 1236 1299 1656
Size: 1167 69 63 357
Fragments arranged by size:
1167 357 69 63
NcoI C'CATG_G
Cuts at: 0 1262 1656
Size: 1262 394
NspI r_CATG'y
Cuts at: 0 165 594 1589 1656
Size: 165 429 995 67
Fragments arranged by size:
995 429 165 67
PciI A'CATG_T
Cuts at: 0 161 1656
Size: 161 1495
PflMI CCAn_nnn'nTGG
Cuts at: 0 699 1656
Size: 699 957
PfoI T'CCnGG_A
Cuts at: 0 1112 1656
Size: 1112 544
PpuMI rG'GwC_Cy
Cuts at: 0 117 552 1627 1656
Size: 117 435 1075 29
Fragments arranged by size:
1075 435 117 29
PspOMI G'GGCC_C
Cuts at: 0 268 1656
Size: 268 1388
PspXI vC'TCGA_Gb
Cuts at: 0 340 1656
Size: 340 1316
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 271 303 1656
Size: 271 32 1353
Fragments arranged by size:
1353 271 32
PstI C_TGCA'G
Cuts at: 0 1003 1166 1656
Size: 1003 163 490
Fragments arranged by size:
1003 490 163
PvuII CAG'CTG
Cuts at: 0 1167 1299 1656
Size: 1167 132 357
Fragments arranged by size:
1167 357 132
SacI G_AGCT'C
Cuts at: 0 632 1656
Size: 632 1024
SanDI GG'GwC_CC
Cuts at: 0 1627 1656
Size: 1627 29
SapI GCTCTTCn'nnn_
Cuts at: 0 658 1656
Size: 658 998
SbfI CC_TGCA'GG
Cuts at: 0 1003 1656
Size: 1003 653
ScaI AGT'ACT
Cuts at: 0 580 1656
Size: 580 1076
SfcI C'TryA_G
Cuts at: 0 822 999 1162 1656
Size: 822 177 163 494
Fragments arranged by size:
822 494 177 163
SmaI CCC'GGG
Cuts at: 0 1426 1656
Size: 1426 230
SmlI C'TyrA_G
Cuts at: 0 121 340 1656
Size: 121 219 1316
Fragments arranged by size:
1316 219 121
SphI G_CATG'C
Cuts at: 0 1589 1656
Size: 1589 67
SrfI GCCC'GGGC
Cuts at: 0 1426 1656
Size: 1426 230
StuI AGG'CCT
Cuts at: 0 835 1622 1656
Size: 835 787 34
Fragments arranged by size:
835 787 34
StyI C'CwwG_G
Cuts at: 0 479 1262 1313 1635 1656
Size: 479 783 51 322 21
Fragments arranged by size:
783 479 322 51 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 415 517 960 1656
Size: 415 102 443 696
Fragments arranged by size:
696 443 415 102
TatI w'GTAC_w
Cuts at: 0 278 578 857 1656
Size: 278 300 279 799
Fragments arranged by size:
799 300 279 278
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1405 1493 1656
Size: 1405 88 163
Fragments arranged by size:
1405 163 88
XhoI C'TCGA_G
Cuts at: 0 340 1656
Size: 340 1316
XmaI C'CCGG_G
Cuts at: 0 1424 1656
Size: 1424 232
ZraI GAC'GTC
Cuts at: 0 213 1656
Size: 213 1443
Enzymes that do cut:
AarI AatII AccI AcuI AflII AflIII AhdI AlwNI
ApaI ApoI AvaI BamHI BanI BanII BclI BlpI
Bme1580I BmrI BplI BpmI BsaAI BsaBI BsaHI BsaWI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrGI BssHII BssSI BstXI BstYI BtgI
BtsI DraIII DrdI EaeI EarI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI FspAI HaeII Hin4I HincII MmeI
MscI MslI MspA1I NcoI NspI PciI PflMI PfoI
PpuMI PspOMI PspXI PsrI PstI PvuII SacI SanDI
SapI SbfI ScaI SfcI SmaI SmlI SphI SrfI
StuI StyI TaqII TatI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AgeI AleI AlfI AloI ApaLI
AscI AseI AsiSI AvrII BaeI BbeI BbsI BbvCI
BcgI BciVI BfrBI BglI BglII BmgBI BmtI Bpu10I
BpuEI BsaI BsaXI BseRI BsiEI BsiWI BsrDI BsrFI
BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI
DraI EagI EciI EcoNI EcoRV FseI HindIII HpaI
KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PmeI PmlI
PpiI PshAI PsiI PvuI RsrII SacII SalI SexAI
SfiI SfoI SgfI SgrAI SnaBI SpeI SspI SwaI
TstI Tth111I XbaI XmnI