Restriction map for
pFN21AE4517
(Linear) (Six-base) MAPSORT of: KIEE4517.seq Check: 5876 from: 1 to: 1650
KIEE4517.orf
With 182 enzymes: SgfI *
March 22, 2012 18:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 48 1650
Size: 48 1602
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 696 862 1650
Size: 696 166 788
Fragments arranged by size:
788 696 166
AgeI A'CCGG_T
Cuts at: 0 494 1650
Size: 494 1156
AleI CACnn'nnGTG
Cuts at: 0 382 1650
Size: 382 1268
AlwNI CAG_nnn'CTG
Cuts at: 0 54 1107 1284 1578 1650
Size: 54 1053 177 294 72
Fragments arranged by size:
1053 294 177 72 54
ApaI G_GGCC'C
Cuts at: 0 245 1650
Size: 245 1405
ApoI r'AATT_y
Cuts at: 0 1056 1650
Size: 1056 594
BamHI G'GATC_C
Cuts at: 0 455 1650
Size: 455 1195
BanI G'GyrC_C
Cuts at: 0 550 1263 1650
Size: 550 713 387
Fragments arranged by size:
713 550 387
BanII G_rGCy'C
Cuts at: 0 245 469 887 1650
Size: 245 224 418 763
Fragments arranged by size:
763 418 245 224
BbsI GAAGACnn'nnnn_
Cuts at: 0 360 1083 1650
Size: 360 723 567
Fragments arranged by size:
723 567 360
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 546 580 1650
Size: 546 34 1070
Fragments arranged by size:
1070 546 34
BglI GCCn_nnn'nGGC
Cuts at: 0 276 1650
Size: 276 1374
BlpI GC'TnA_GC
Cuts at: 0 517 1650
Size: 517 1133
Bme1580I G_kGCm'C
Cuts at: 0 245 553 794 1019 1650
Size: 245 308 241 225 631
Fragments arranged by size:
631 308 245 241 225
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 135 990 1650
Size: 135 855 660
Fragments arranged by size:
855 660 135
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 333 757 783 1050 1114 1600 1650
Size: 130 203 424 26 267 64 486 50
Fragments arranged by size:
486 424 267 203 130 64 50 26
Bpu10I CC'TnA_GC
Cuts at: 0 430 1650
Size: 430 1220
BsaI GGTCTCn'nnnn_
Cuts at: 0 1534 1650
Size: 1534 116
BsaWI w'CCGG_w
Cuts at: 0 494 1650
Size: 494 1156
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 976 1006 1541 1571 1650
Size: 976 30 535 30 79
Fragments arranged by size:
976 535 79 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 475 979 1047 1117 1469 1474 1650
Size: 475 504 68 70 352 5 176
Fragments arranged by size:
504 475 352 176 70 68 5
BseYI C'CCAG_C
Cuts at: 0 347 732 1305 1419 1650
Size: 347 385 573 114 231
Fragments arranged by size:
573 385 347 231 114
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 426 1650
Size: 426 1224
BsiEI CG_ry'CG
Cuts at: 0 994 1650
Size: 994 656
BsiHKAI G_wGCw'C
Cuts at: 0 65 887 1025 1650
Size: 65 822 138 625
Fragments arranged by size:
822 625 138 65
BsmI GAATG_Cn'
Cuts at: 0 1397 1650
Size: 1397 253
Bsp1286I G_dGCh'C
Cuts at: 0 65 245 469 553 794 887 1019 1025
Size: 65 180 224 84 241 93 132 6
Cuts at: 1025 1650
Size: 625
Fragments arranged by size:
625 241 224 180 132 93 84 65
6
BspHI T'CATG_A
Cuts at: 0 86 1650
Size: 86 1564
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 48 1106 1345 1650
Size: 48 1058 239 305
Fragments arranged by size:
1058 305 239 48
BsrBI CCG'CTC
Cuts at: 0 1557 1650
Size: 1557 93
BsrDI GCAATG_nn'
Cuts at: 0 1502 1650
Size: 1502 148
BsrFI r'CCGG_y
Cuts at: 0 494 622 1234 1415 1613 1650
Size: 494 128 612 181 198 37
Fragments arranged by size:
612 494 198 181 128 37
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1275 1284 1650
Size: 1275 9 366
Fragments arranged by size:
1275 366 9
BstEII G'GTnAC_C
Cuts at: 0 684 1650
Size: 684 966
BstXI CCAn_nnnn'nTGG
Cuts at: 0 384 696 1050 1260 1650
Size: 384 312 354 210 390
Fragments arranged by size:
390 384 354 312 210
BstYI r'GATC_y
Cuts at: 0 455 1650
Size: 455 1195
BtgI C'CryG_G
Cuts at: 0 299 542 988 1148 1229 1650
Size: 299 243 446 160 81 421
Fragments arranged by size:
446 421 299 243 160 81
BtsI GCAGTG_nn'
Cuts at: 0 1172 1650
Size: 1172 478
DrdI GACnn_nn'nnGTC
Cuts at: 0 349 364 1650
Size: 349 15 1286
Fragments arranged by size:
1286 349 15
EaeI y'GGCC_r
Cuts at: 0 302 386 539 605 620 896 909 1197
Size: 302 84 153 66 15 276 13 288
Cuts at: 1197 1232 1650
Size: 35 418
Fragments arranged by size:
418 302 288 276 153 84 66 35
15 13
EarI CTCTTCn'nnn_
Cuts at: 0 49 1144 1650
Size: 49 1095 506
Fragments arranged by size:
1095 506 49
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 253 825 1650
Size: 253 572 825
Fragments arranged by size:
825 572 253
EcoICRI GAG'CTC
Cuts at: 0 885 1650
Size: 885 765
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 333 696 757 783 862 1050 1114
Size: 130 203 363 61 26 79 188 64
Cuts at: 1114 1600 1650
Size: 486 50
Fragments arranged by size:
486 363 203 188 130 79 64 61
50 26
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1129 1650
Size: 1129 521
EcoO109I rG'GnC_Cy
Cuts at: 0 201 229 241 242 396 480 1134 1435
Size: 201 28 12 1 154 84 654 301
Cuts at: 1435 1575 1650
Size: 140 75
Fragments arranged by size:
654 301 201 154 140 84 75 28
12 1
EcoRV GAT'ATC
Cuts at: 0 815 1650
Size: 815 835
FseI GG_CCGG'CC
Cuts at: 0 1238 1650
Size: 1238 412
HaeII r_GCGC'y
Cuts at: 0 733 1650
Size: 733 917
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 154 186 976 1008 1541 1573 1650
Size: 154 32 790 32 533 32 77
Fragments arranged by size:
790 533 154 77 32 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1393 1650
Size: 1393 257
MscI TGG'CCA
Cuts at: 0 388 898 911 1650
Size: 388 510 13 739
Fragments arranged by size:
739 510 388 13
MslI CAynn'nnrTG
Cuts at: 0 187 382 445 694 940 1048 1261 1650
Size: 187 195 63 249 246 108 213 389
Fragments arranged by size:
389 249 246 213 195 187 108 63
MspA1I CmG'CkG
Cuts at: 0 311 777 990 1028 1650
Size: 311 466 213 38 622
Fragments arranged by size:
622 466 311 213 38
NaeI GCC'GGC
Cuts at: 0 624 1236 1417 1650
Size: 624 612 181 233
Fragments arranged by size:
624 612 233 181
NcoI C'CATG_G
Cuts at: 0 1229 1650
Size: 1229 421
NgoMIV G'CCGG_C
Cuts at: 0 622 1234 1415 1650
Size: 622 612 181 235
Fragments arranged by size:
622 612 235 181
NspI r_CATG'y
Cuts at: 0 159 1260 1650
Size: 159 1101 390
Fragments arranged by size:
1101 390 159
PasI CC'CwG_GG
Cuts at: 0 414 1545 1605 1650
Size: 414 1131 60 45
Fragments arranged by size:
1131 414 60 45
PflMI CCAn_nnn'nTGG
Cuts at: 0 311 1230 1578 1650
Size: 311 919 348 72
Fragments arranged by size:
919 348 311 72
PfoI T'CCnGG_A
Cuts at: 0 107 451 1535 1571 1650
Size: 107 344 1084 36 79
Fragments arranged by size:
1084 344 107 79 36
PpuMI rG'GwC_Cy
Cuts at: 0 396 1435 1575 1650
Size: 396 1039 140 75
Fragments arranged by size:
1039 396 140 75
PspOMI G'GGCC_C
Cuts at: 0 241 1650
Size: 241 1409
PstI C_TGCA'G
Cuts at: 0 59 536 1103 1650
Size: 59 477 567 547
Fragments arranged by size:
567 547 477 59
PvuII CAG'CTG
Cuts at: 0 311 777 1650
Size: 311 466 873
Fragments arranged by size:
873 466 311
SacI G_AGCT'C
Cuts at: 0 887 1650
Size: 887 763
SacII CC_GC'GG
Cuts at: 0 991 1650
Size: 991 659
SanDI GG'GwC_CC
Cuts at: 0 1435 1650
Size: 1435 215
SbfI CC_TGCA'GG
Cuts at: 0 59 1650
Size: 59 1591
SexAI A'CCwGG_T
Cuts at: 0 398 1650
Size: 398 1252
SfcI C'TryA_G
Cuts at: 0 55 532 1099 1650
Size: 55 477 567 551
Fragments arranged by size:
567 551 477 55
StyI C'CwwG_G
Cuts at: 0 197 1194 1229 1650
Size: 197 997 35 421
Fragments arranged by size:
997 421 197 35
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 388 420 1650
Size: 388 32 1230
Fragments arranged by size:
1230 388 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 396 696 1650
Size: 396 300 954
Fragments arranged by size:
954 396 300
XmnI GAAnn'nnTTC
Cuts at: 0 1364 1650
Size: 1364 286
Enzymes that do cut:
AarI AcuI AgeI AleI AlwNI ApaI ApoI BamHI
BanI BanII BbsI BcgI BglI BlpI Bme1580I BplI
BpmI Bpu10I BsaI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BstAPI BstEII BstXI BstYI BtgI BtsI DrdI
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV
FseI HaeII Hin4I MmeI MscI MslI MspA1I NaeI
NcoI NgoMIV NspI PasI PflMI PfoI PpuMI PspOMI
PstI PvuII SacI SacII SanDI SbfI SexAI SfcI
StyI TstI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AhdI
AlfI AloI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BbeI BbvCI BciVI BclI BfrBI BglII BmgBI
BmrI BmtI BpuEI BsaAI BsaBI BsaHI BsiWI BsmBI
BspEI BsrGI BssHII BssSI BstBI BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DraIII EagI EcoRI FalI FspI
FspAI HincII HindIII HpaI KasI KpnI MfeI MluI
NarI NdeI NheI NotI NruI NsiI PacI PciI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SalI SapI ScaI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TaqII TatI Tth111I XbaI XhoI XmaI ZraI