Restriction map for
pFN21AE4512
(Linear) (Six-base) MAPSORT of: KIEE4512.seq Check: 1224 from: 1 to: 1620
KIEE4512.orf
With 182 enzymes: SgfI *
March 22, 2012 18:14 ..
AfeI AGC'GCT
Cuts at: 0 1039 1620
Size: 1039 581
AgeI A'CCGG_T
Cuts at: 0 461 1620
Size: 461 1159
AleI CACnn'nnGTG
Cuts at: 0 1274 1620
Size: 1274 346
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 781 813 1015 1047 1620
Size: 781 32 202 32 573
Fragments arranged by size:
781 573 202 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 424 982 1620
Size: 424 558 638
Fragments arranged by size:
638 558 424
AvaI C'yCGr_G
Cuts at: 0 876 1172 1620
Size: 876 296 448
Fragments arranged by size:
876 448 296
BanI G'GyrC_C
Cuts at: 0 906 1620
Size: 906 714
BanII G_rGCy'C
Cuts at: 0 1200 1573 1610 1620
Size: 1200 373 37 10
Fragments arranged by size:
1200 373 37 10
BbsI GAAGACnn'nnnn_
Cuts at: 0 1135 1620
Size: 1135 485
BbvCI CC'TCA_GC
Cuts at: 0 97 1620
Size: 97 1523
BciVI GTATCCnnnnn_n'
Cuts at: 0 1083 1620
Size: 1083 537
BglI GCCn_nnn'nGGC
Cuts at: 0 1195 1620
Size: 1195 425
Bme1580I G_kGCm'C
Cuts at: 0 911 1620
Size: 911 709
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 369 1587 1620
Size: 369 1218 33
Fragments arranged by size:
1218 369 33
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 343 503 658 1042 1553 1598 1620
Size: 343 160 155 384 511 45 22
Fragments arranged by size:
511 384 343 160 155 45 22
Bpu10I CC'TnA_GC
Cuts at: 0 97 1620
Size: 97 1523
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 904 1620
Size: 904 716
BsaI GGTCTCn'nnnn_
Cuts at: 0 616 1436 1620
Size: 616 820 184
Fragments arranged by size:
820 616 184
BsaWI w'CCGG_w
Cuts at: 0 461 1620
Size: 461 1159
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 166 196 781 811 1015 1045 1620
Size: 166 30 585 30 204 30 575
Fragments arranged by size:
585 575 204 166 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 174 1399 1402 1620
Size: 174 1225 3 218
Fragments arranged by size:
1225 218 174 3
BseYI C'CCAG_C
Cuts at: 0 443 476 824 853 1162 1620
Size: 443 33 348 29 309 458
Fragments arranged by size:
458 443 348 309 33 29
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 759 1400 1620
Size: 244 515 641 220
Fragments arranged by size:
641 515 244 220
BsiHKAI G_wGCw'C
Cuts at: 0 110 1620
Size: 110 1510
BsmI GAATG_Cn'
Cuts at: 0 392 441 1620
Size: 392 49 1179
Fragments arranged by size:
1179 392 49
Bsp1286I G_dGCh'C
Cuts at: 0 110 911 1200 1573 1610 1620
Size: 110 801 289 373 37 10
Fragments arranged by size:
801 373 289 110 37 10
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 285 980 1361 1620
Size: 285 695 381 259
Fragments arranged by size:
695 381 285 259
BsrBI CCG'CTC
Cuts at: 0 956 1620
Size: 956 664
BsrDI GCAATG_nn'
Cuts at: 0 725 1620
Size: 725 895
BsrFI r'CCGG_y
Cuts at: 0 461 485 1620
Size: 461 24 1135
Fragments arranged by size:
1135 461 24
BsrGI T'GTAC_A
Cuts at: 0 1069 1620
Size: 1069 551
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1263 1276 1620
Size: 1263 13 344
Fragments arranged by size:
1263 344 13
Bsu36I CC'TnA_GG
Cuts at: 0 320 943 1620
Size: 320 623 677
Fragments arranged by size:
677 623 320
BtgI C'CryG_G
Cuts at: 0 167 1620
Size: 167 1453
BtsI GCAGTG_nn'
Cuts at: 0 447 663 1620
Size: 447 216 957
Fragments arranged by size:
957 447 216
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1129 1164 1620
Size: 1129 35 456
Fragments arranged by size:
1129 456 35
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 343 503 658 1042 1553 1598 1620
Size: 343 160 155 384 511 45 22
Fragments arranged by size:
511 384 343 160 155 45 22
EcoO109I rG'GnC_Cy
Cuts at: 0 307 1620
Size: 307 1313
HaeII r_GCGC'y
Cuts at: 0 951 1041 1620
Size: 951 90 579
Fragments arranged by size:
951 579 90
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 859 891 1418 1450 1620
Size: 859 32 527 32 170
Fragments arranged by size:
859 527 170 32 32
HincII GTy'rAC
Cuts at: 0 1488 1620
Size: 1488 132
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 282 1620
Size: 282 1338
MslI CAynn'nnrTG
Cuts at: 0 368 1274 1620
Size: 368 906 346
Fragments arranged by size:
906 368 346
MspA1I CmG'CkG
Cuts at: 0 96 656 1020 1343 1620
Size: 96 560 364 323 277
Fragments arranged by size:
560 364 323 277 96
NcoI C'CATG_G
Cuts at: 0 167 1620
Size: 167 1453
NspI r_CATG'y
Cuts at: 0 562 1620
Size: 562 1058
PasI CC'CwG_GG
Cuts at: 0 505 1620
Size: 505 1115
PflMI CCAn_nnn'nTGG
Cuts at: 0 489 1620
Size: 489 1131
PfoI T'CCnGG_A
Cuts at: 0 430 635 809 1620
Size: 430 205 174 811
Fragments arranged by size:
811 430 205 174
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 727 759 781 813 1015 1047 1620
Size: 727 32 22 32 202 32 573
Fragments arranged by size:
727 573 202 32 32 32 22
PpuMI rG'GwC_Cy
Cuts at: 0 307 1620
Size: 307 1313
PshAI GACnn'nnGTC
Cuts at: 0 1241 1620
Size: 1241 379
PvuII CAG'CTG
Cuts at: 0 96 1020 1620
Size: 96 924 600
Fragments arranged by size:
924 600 96
SanDI GG'GwC_CC
Cuts at: 0 307 1620
Size: 307 1313
SfcI C'TryA_G
Cuts at: 0 80 1620
Size: 80 1540
SmlI C'TyrA_G
Cuts at: 0 883 1620
Size: 883 737
StuI AGG'CCT
Cuts at: 0 1081 1372 1620
Size: 1081 291 248
Fragments arranged by size:
1081 291 248
StyI C'CwwG_G
Cuts at: 0 23 167 468 1550 1610 1620
Size: 23 144 301 1082 60 10
Fragments arranged by size:
1082 301 144 60 23 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 738 1620
Size: 738 882
TatI w'GTAC_w
Cuts at: 0 1069 1620
Size: 1069 551
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 166 198 1620
Size: 166 32 1422
Fragments arranged by size:
1422 166 32
Tth111I GACn'n_nGTC
Cuts at: 0 215 1620
Size: 215 1405
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 21 490 1620
Size: 21 469 1130
Fragments arranged by size:
1130 469 21
Enzymes that do cut:
AfeI AgeI AleI AloI AlwNI AvaI BanI BanII
BbsI BbvCI BciVI BglI Bme1580I BplI BpmI Bpu10I
BpuEI BsaI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BstXI
Bsu36I BtgI BtsI CspCI Eco57MI EcoO109I HaeII Hin4I
HincII MmeI MslI MspA1I NcoI NspI PasI PflMI
PfoI PpiI PpuMI PshAI PvuII SanDI SfcI SmlI
StuI StyI TaqII TatI TstI Tth111I XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AhdI AlfI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BcgI BclI BfrBI BglII
BlpI BmgBI BmrI BmtI BsaAI BsaBI BsaHI BsiEI
BsiWI BsmBI BspEI BspHI BssHII BssSI BstAPI BstBI
BstEII BstYI BstZ17I BtgZI ClaI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HindIII HpaI KasI KpnI
MfeI MluI MscI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PciI PmeI PmlI PsiI
PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI
XbaI XhoI XmaI XmnI ZraI