Restriction map for
pFN21AE4511
(Linear) (Six-base) MAPSORT of: KIEE4511.seq Check: 3491 from: 1 to: 1617
KIEE4511.orf
With 182 enzymes: SgfI *
March 22, 2012 18:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 954 1617
Size: 954 663
AccI GT'mk_AC
Cuts at: 0 84 1617
Size: 84 1533
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1153 1617
Size: 1153 464
AflIII A'CryG_T
Cuts at: 0 821 1617
Size: 821 796
ApaI G_GGCC'C
Cuts at: 0 708 834 1617
Size: 708 126 783
Fragments arranged by size:
783 708 126
ApoI r'AATT_y
Cuts at: 0 316 400 666 714 866 1475 1617
Size: 316 84 266 48 152 609 142
Fragments arranged by size:
609 316 266 152 142 84 48
AseI AT'TA_AT
Cuts at: 0 1397 1617
Size: 1397 220
BamHI G'GATC_C
Cuts at: 0 204 1617
Size: 204 1413
BanII G_rGCy'C
Cuts at: 0 708 834 1116 1617
Size: 708 126 282 501
Fragments arranged by size:
708 501 282 126
BclI T'GATC_A
Cuts at: 0 291 1617
Size: 291 1326
BglI GCCn_nnn'nGGC
Cuts at: 0 703 1617
Size: 703 914
Bme1580I G_kGCm'C
Cuts at: 0 579 708 834 1617
Size: 579 129 126 783
Fragments arranged by size:
783 579 129 126
BmgBI CAC'GTC
Cuts at: 0 822 1617
Size: 822 795
BmrI ACTGGGnnnn_n'
Cuts at: 0 515 1617
Size: 515 1102
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 908 1223 1617
Size: 908 315 394
Fragments arranged by size:
908 394 315
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 56 1617
Size: 56 1561
BsaBI GATnn'nnATC
Cuts at: 0 523 1617
Size: 523 1094
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 728 1617
Size: 728 889
BseYI C'CCAG_C
Cuts at: 0 1534 1617
Size: 1534 83
BsiHKAI G_wGCw'C
Cuts at: 0 1116 1413 1617
Size: 1116 297 204
Fragments arranged by size:
1116 297 204
BsmI GAATG_Cn'
Cuts at: 0 613 1617
Size: 613 1004
Bsp1286I G_dGCh'C
Cuts at: 0 579 708 834 1116 1413 1617
Size: 579 129 126 282 297 204
Fragments arranged by size:
579 297 282 204 129 126
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 954 1617
Size: 954 663
BsrGI T'GTAC_A
Cuts at: 0 384 1617
Size: 384 1233
BstYI r'GATC_y
Cuts at: 0 204 1617
Size: 204 1413
Bsu36I CC'TnA_GG
Cuts at: 0 1547 1617
Size: 1547 70
BtgI C'CryG_G
Cuts at: 0 8 1617
Size: 8 1609
BtsI GCAGTG_nn'
Cuts at: 0 804 1097 1617
Size: 804 293 520
Fragments arranged by size:
804 520 293
DraI TTT'AAA
Cuts at: 0 1334 1617
Size: 1334 283
DraIII CAC_nnn'GTG
Cuts at: 0 1288 1617
Size: 1288 329
EarI CTCTTCn'nnn_
Cuts at: 0 84 1617
Size: 84 1533
EcoICRI GAG'CTC
Cuts at: 0 1114 1617
Size: 1114 503
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 908 1153 1223 1617
Size: 908 245 70 394
Fragments arranged by size:
908 394 245 70
EcoO109I rG'GnC_Cy
Cuts at: 0 705 831 1617
Size: 705 126 786
Fragments arranged by size:
786 705 126
MslI CAynn'nnrTG
Cuts at: 0 551 1617
Size: 551 1066
NcoI C'CATG_G
Cuts at: 0 8 1617
Size: 8 1609
NdeI CA'TA_TG
Cuts at: 0 1428 1617
Size: 1428 189
PflMI CCAn_nnn'nTGG
Cuts at: 0 1603 1617
Size: 1603 14
PsiI TTA'TAA
Cuts at: 0 50 932 1617
Size: 50 882 685
Fragments arranged by size:
882 685 50
PspOMI G'GGCC_C
Cuts at: 0 704 830 1617
Size: 704 126 787
Fragments arranged by size:
787 704 126
SacI G_AGCT'C
Cuts at: 0 1116 1617
Size: 1116 501
SfcI C'TryA_G
Cuts at: 0 33 254 1617
Size: 33 221 1363
Fragments arranged by size:
1363 221 33
SmlI C'TyrA_G
Cuts at: 0 71 1617
Size: 71 1546
SspI AAT'ATT
Cuts at: 0 588 969 1143 1350 1617
Size: 588 381 174 207 267
Fragments arranged by size:
588 381 267 207 174
StyI C'CwwG_G
Cuts at: 0 8 488 1617
Size: 8 480 1129
Fragments arranged by size:
1129 480 8
TatI w'GTAC_w
Cuts at: 0 384 1617
Size: 384 1233
XmnI GAAnn'nnTTC
Cuts at: 0 554 638 1617
Size: 554 84 979
Fragments arranged by size:
979 554 84
Enzymes that do cut:
AarI AccI AcuI AflIII ApaI ApoI AseI BamHI
BanII BclI BglI Bme1580I BmgBI BmrI BpmI BpuEI
BsaBI BseRI BseYI BsiHKAI BsmI Bsp1286I BspMI BsrGI
BstYI Bsu36I BtgI BtsI DraI DraIII EarI EcoICRI
Eco57MI EcoO109I MslI NcoI NdeI PflMI PsiI PspOMI
SacI SfcI SmlI SspI StyI TatI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI AloI AlwNI ApaLI AscI AsiSI AvaI AvrII
BaeI BanI BbeI BbsI BbvCI BcgI BciVI BfrBI
BglII BlpI BmtI BplI Bpu10I BsaI BsaAI BsaHI
BsaWI BsaXI BsgI BsiEI BsiWI BsmBI BspEI BspHI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII
BstXI BstZ17I BtgZI ClaI CspCI DrdI EaeI EagI
EciI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MspA1I NaeI NarI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PfoI
PmeI PmlI PpiI PpuMI PshAI PspXI PsrI PstI
PvuI PvuII RsrII SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI StuI SwaI TaqII TstI Tth111I
XbaI XcmI XhoI XmaI ZraI