Restriction map for
pFN21AE4510
(Linear) (Six-base) MAPSORT of: KIEE4510.seq Check: 2314 from: 1 to: 1596
KIEE4510.orf
With 182 enzymes: SgfI *
July 3, 2012 19:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 94 1596
Size: 94 1502
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 1498 1564 1596
Size: 322 1176 66 32
Fragments arranged by size:
1176 322 66 32
AgeI A'CCGG_T
Cuts at: 0 1472 1596
Size: 1472 124
AhdI GACnn_n'nnGTC
Cuts at: 0 1152 1596
Size: 1152 444
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 797 1596
Size: 797 799
AlwNI CAG_nnn'CTG
Cuts at: 0 59 1596
Size: 59 1537
ApaI G_GGCC'C
Cuts at: 0 1426 1596
Size: 1426 170
ApoI r'AATT_y
Cuts at: 0 124 579 913 1596
Size: 124 455 334 683
Fragments arranged by size:
683 455 334 124
AseI AT'TA_AT
Cuts at: 0 1226 1596
Size: 1226 370
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 311 344 1596
Size: 311 33 1252
Fragments arranged by size:
1252 311 33
BanII G_rGCy'C
Cuts at: 0 83 863 1426 1596
Size: 83 780 563 170
Fragments arranged by size:
780 563 170 83
BbsI GAAGACnn'nnnn_
Cuts at: 0 1214 1596
Size: 1214 382
BciVI GTATCCnnnnn_n'
Cuts at: 0 339 464 1596
Size: 339 125 1132
Fragments arranged by size:
1132 339 125
BclI T'GATC_A
Cuts at: 0 632 1596
Size: 632 964
Bme1580I G_kGCm'C
Cuts at: 0 1426 1596
Size: 1426 170
BmrI ACTGGGnnnn_n'
Cuts at: 0 1458 1596
Size: 1458 138
BmtI G_CTAG'C
Cuts at: 0 158 1596
Size: 158 1438
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 1135 1596
Size: 97 1038 461
Fragments arranged by size:
1038 461 97
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 712 1102 1596
Size: 712 390 494
Fragments arranged by size:
712 494 390
BsaI GGTCTCn'nnnn_
Cuts at: 0 916 1264 1596
Size: 916 348 332
Fragments arranged by size:
916 348 332
BsaWI w'CCGG_w
Cuts at: 0 439 1472 1596
Size: 439 1033 124
Fragments arranged by size:
1033 439 124
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 17 47 1596
Size: 17 30 1549
Fragments arranged by size:
1549 30 17
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 157 1064 1302 1596
Size: 157 907 238 294
Fragments arranged by size:
907 294 238 157
BseYI C'CCAG_C
Cuts at: 0 864 1160 1596
Size: 864 296 436
Fragments arranged by size:
864 436 296
BsiEI CG_ry'CG
Cuts at: 0 1477 1596
Size: 1477 119
BsiHKAI G_wGCw'C
Cuts at: 0 863 1596
Size: 863 733
BsmI GAATG_Cn'
Cuts at: 0 786 872 1596
Size: 786 86 724
Fragments arranged by size:
786 724 86
Bsp1286I G_dGCh'C
Cuts at: 0 83 863 1426 1596
Size: 83 780 563 170
Fragments arranged by size:
780 563 170 83
BspEI T'CCGG_A
Cuts at: 0 439 1596
Size: 439 1157
BspHI T'CATG_A
Cuts at: 0 629 1596
Size: 629 967
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 94 1596
Size: 94 1502
BsrDI GCAATG_nn'
Cuts at: 0 108 272 1596
Size: 108 164 1324
Fragments arranged by size:
1324 164 108
BsrFI r'CCGG_y
Cuts at: 0 1472 1596
Size: 1472 124
BsrGI T'GTAC_A
Cuts at: 0 764 1596
Size: 764 832
BssSI C'ACGA_G
Cuts at: 0 1496 1596
Size: 1496 100
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1123 1433 1596
Size: 1123 310 163
Fragments arranged by size:
1123 310 163
BstYI r'GATC_y
Cuts at: 0 257 1359 1596
Size: 257 1102 237
Fragments arranged by size:
1102 257 237
Bsu36I CC'TnA_GG
Cuts at: 0 832 1596
Size: 832 764
BtgI C'CryG_G
Cuts at: 0 1342 1373 1596
Size: 1342 31 223
Fragments arranged by size:
1342 223 31
BtsI GCAGTG_nn'
Cuts at: 0 1106 1438 1596
Size: 1106 332 158
Fragments arranged by size:
1106 332 158
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 72 107 1596
Size: 72 35 1489
Fragments arranged by size:
1489 72 35
DraI TTT'AAA
Cuts at: 0 498 821 1059 1596
Size: 498 323 238 537
Fragments arranged by size:
537 498 323 238
EaeI y'GGCC_r
Cuts at: 0 14 366 929 1370 1435 1596
Size: 14 352 563 441 65 161
Fragments arranged by size:
563 441 352 161 65 14
EarI CTCTTCn'nnn_
Cuts at: 0 62 91 189 1596
Size: 62 29 98 1407
Fragments arranged by size:
1407 98 62 29
EcoICRI GAG'CTC
Cuts at: 0 861 1596
Size: 861 735
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 322 1135 1498 1564 1596
Size: 97 225 813 363 66 32
Fragments arranged by size:
813 363 225 97 66 32
EcoNI CCTnn'n_nnAGG
Cuts at: 0 51 1596
Size: 51 1545
EcoO109I rG'GnC_Cy
Cuts at: 0 114 1596
Size: 114 1482
EcoRI G'AATT_C
Cuts at: 0 579 913 1596
Size: 579 334 683
Fragments arranged by size:
683 579 334
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1542 1596
Size: 1542 54
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1138 1170 1294 1326 1596
Size: 1138 32 124 32 270
Fragments arranged by size:
1138 270 124 32 32
HincII GTy'rAC
Cuts at: 0 1091 1146 1592 1596
Size: 1091 55 446 4
Fragments arranged by size:
1091 446 55 4
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 203 1596
Size: 203 1393
MscI TGG'CCA
Cuts at: 0 16 368 1372 1437 1596
Size: 16 352 1004 65 159
Fragments arranged by size:
1004 352 159 65 16
MslI CAynn'nnrTG
Cuts at: 0 1099 1596
Size: 1099 497
MspA1I CmG'CkG
Cuts at: 0 1160 1596
Size: 1160 436
NcoI C'CATG_G
Cuts at: 0 1342 1373 1596
Size: 1342 31 223
Fragments arranged by size:
1342 223 31
NheI G'CTAG_C
Cuts at: 0 154 1596
Size: 154 1442
NspI r_CATG'y
Cuts at: 0 699 1596
Size: 699 897
PasI CC'CwG_GG
Cuts at: 0 1426 1596
Size: 1426 170
PfoI T'CCnGG_A
Cuts at: 0 260 480 1596
Size: 260 220 1116
Fragments arranged by size:
1116 260 220
PspOMI G'GGCC_C
Cuts at: 0 1422 1596
Size: 1422 174
SacI G_AGCT'C
Cuts at: 0 863 1596
Size: 863 733
SapI GCTCTTCn'nnn_
Cuts at: 0 91 1596
Size: 91 1505
SexAI A'CCwGG_T
Cuts at: 0 534 1596
Size: 534 1062
SmlI C'TyrA_G
Cuts at: 0 727 1081 1596
Size: 727 354 515
Fragments arranged by size:
727 515 354
StyI C'CwwG_G
Cuts at: 0 688 1122 1342 1373 1596
Size: 688 434 220 31 223
Fragments arranged by size:
688 434 223 220 31
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 645 1596
Size: 645 951
TatI w'GTAC_w
Cuts at: 0 305 764 1189 1596
Size: 305 459 425 407
Fragments arranged by size:
459 425 407 305
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 17 49 434 466 1596
Size: 17 32 385 32 1130
Fragments arranged by size:
1130 385 32 32 17
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1371 1596
Size: 1371 225
XmnI GAAnn'nnTTC
Cuts at: 0 913 1596
Size: 913 683
Enzymes that do cut:
AarI AcuI AgeI AhdI AlfI AlwNI ApaI ApoI
AseI BaeI BanII BbsI BciVI BclI Bme1580I BmrI
BmtI BpmI BpuEI BsaI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrDI
BsrFI BsrGI BssSI BstXI BstYI Bsu36I BtgI BtsI
CspCI DraI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI Hin4I HincII MmeI MscI MslI MspA1I
NcoI NheI NspI PasI PfoI PspOMI SacI SapI
SexAI SmlI StyI TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AleI
AloI ApaLI AscI AsiSI AvaI AvrII BamHI BanI
BbeI BbvCI BcgI BfrBI BglI BglII BlpI BmgBI
BplI Bpu10I BsaAI BsaBI BsaHI BsgI BsiWI BsmBI
BsrBI BssHII BstAPI BstBI BstEII BstZ17I BtgZI ClaI
DraIII DrdI EagI EciI EcoRV FseI FspI FspAI
HaeII HindIII HpaI KasI KpnI MfeI MluI NaeI
NarI NdeI NgoMIV NotI NruI NsiI PacI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI
PsrI PstI PvuI PvuII RsrII SacII SalI SanDI
SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I
XbaI XhoI XmaI ZraI