Restriction map for
pFN21AE4509
(Linear) (Six-base) MAPSORT of: KIEE4509.seq Check: 1935 from: 1 to: 1596
KIEE4509.orf
With 182 enzymes: SgfI *
March 22, 2012 18:14 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 427 1596
Size: 427 1169
AfeI AGC'GCT
Cuts at: 0 39 128 1596
Size: 39 89 1468
Fragments arranged by size:
1468 89 39
AleI CACnn'nnGTG
Cuts at: 0 1378 1381 1596
Size: 1378 3 215
Fragments arranged by size:
1378 215 3
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 117 1481 1596
Size: 117 1364 115
Fragments arranged by size:
1364 117 115
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 917 949 1596
Size: 917 32 647
Fragments arranged by size:
917 647 32
ApaI G_GGCC'C
Cuts at: 0 65 1596
Size: 65 1531
AvaI C'yCGr_G
Cuts at: 0 328 471 1596
Size: 328 143 1125
Fragments arranged by size:
1125 328 143
BamHI G'GATC_C
Cuts at: 0 424 1596
Size: 424 1172
BanI G'GyrC_C
Cuts at: 0 119 301 1596
Size: 119 182 1295
Fragments arranged by size:
1295 182 119
BanII G_rGCy'C
Cuts at: 0 65 72 194 1202 1596
Size: 65 7 122 1008 394
Fragments arranged by size:
1008 394 122 65 7
BbeI G_GCGC'C
Cuts at: 0 123 305 1596
Size: 123 182 1291
Fragments arranged by size:
1291 182 123
BbsI GAAGACnn'nnnn_
Cuts at: 0 740 1596
Size: 740 856
BbvCI CC'TCA_GC
Cuts at: 0 1469 1596
Size: 1469 127
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 333 367 759 793 1596
Size: 333 34 392 34 803
Fragments arranged by size:
803 392 333 34 34
BclI T'GATC_A
Cuts at: 0 263 1596
Size: 263 1333
BlpI GC'TnA_GC
Cuts at: 0 193 1035 1596
Size: 193 842 561
Fragments arranged by size:
842 561 193
Bme1580I G_kGCm'C
Cuts at: 0 65 1566 1596
Size: 65 1501 30
Fragments arranged by size:
1501 65 30
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 754 1300 1596
Size: 754 546 296
Fragments arranged by size:
754 546 296
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 484 1014 1295 1324 1596
Size: 484 530 281 29 272
Fragments arranged by size:
530 484 281 272 29
Bpu10I CC'TnA_GC
Cuts at: 0 41 199 1469 1596
Size: 41 158 1270 127
Fragments arranged by size:
1270 158 127 41
BsaI GGTCTCn'nnnn_
Cuts at: 0 10 1596
Size: 10 1586
BsaAI yAC'GTr
Cuts at: 0 569 804 1596
Size: 569 235 792
Fragments arranged by size:
792 569 235
BsaHI Gr'CG_yC
Cuts at: 0 120 302 333 1596
Size: 120 182 31 1263
Fragments arranged by size:
1263 182 120 31
BsaWI w'CCGG_w
Cuts at: 0 427 1367 1596
Size: 427 940 229
Fragments arranged by size:
940 427 229
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 917 947 1596
Size: 917 30 649
Fragments arranged by size:
917 649 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 470 985 1522 1525 1596
Size: 470 515 537 3 71
Fragments arranged by size:
537 515 470 71 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1430 1596
Size: 1430 166
BsiEI CG_ry'CG
Cuts at: 0 59 1596
Size: 59 1537
BsiHKAI G_wGCw'C
Cuts at: 0 72 1202 1596
Size: 72 1130 394
Fragments arranged by size:
1130 394 72
BsiWI C'GTAC_G
Cuts at: 0 565 1596
Size: 565 1031
BsmI GAATG_Cn'
Cuts at: 0 1415 1596
Size: 1415 181
BsmBI CGTCTCn'nnnn_
Cuts at: 0 490 1596
Size: 490 1106
Bsp1286I G_dGCh'C
Cuts at: 0 65 72 194 1202 1566 1596
Size: 65 7 122 1008 364 30
Fragments arranged by size:
1008 364 122 65 30 7
BspHI T'CATG_A
Cuts at: 0 1326 1596
Size: 1326 270
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 201 1033 1091 1340 1596
Size: 201 832 58 249 256
Fragments arranged by size:
832 256 249 201 58
BsrDI GCAATG_nn'
Cuts at: 0 1175 1289 1596
Size: 1175 114 307
Fragments arranged by size:
1175 307 114
BstEII G'GTnAC_C
Cuts at: 0 213 870 1596
Size: 213 657 726
Fragments arranged by size:
726 657 213
BstXI CCAn_nnnn'nTGG
Cuts at: 0 456 549 1302 1380 1596
Size: 456 93 753 78 216
Fragments arranged by size:
753 456 216 93 78
BstYI r'GATC_y
Cuts at: 0 424 632 1596
Size: 424 208 964
Fragments arranged by size:
964 424 208
BtgI C'CryG_G
Cuts at: 0 80 395 1166 1376 1553 1596
Size: 80 315 771 210 177 43
Fragments arranged by size:
771 315 210 177 80 43
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 330 1502 1596
Size: 330 1172 94
Fragments arranged by size:
1172 330 94
DraIII CAC_nnn'GTG
Cuts at: 0 379 1596
Size: 379 1217
DrdI GACnn_nn'nnGTC
Cuts at: 0 444 1596
Size: 444 1152
EaeI y'GGCC_r
Cuts at: 0 56 521 1163 1556 1596
Size: 56 465 642 393 40
Fragments arranged by size:
642 465 393 56 40
EagI C'GGCC_G
Cuts at: 0 56 1596
Size: 56 1540
EarI CTCTTCn'nnn_
Cuts at: 0 1 1064 1596
Size: 1 1063 532
Fragments arranged by size:
1063 532 1
EcoICRI GAG'CTC
Cuts at: 0 70 1200 1596
Size: 70 1130 396
Fragments arranged by size:
1130 396 70
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 427 484 1014 1295 1324 1596
Size: 427 57 530 281 29 272
Fragments arranged by size:
530 427 281 272 57 29
EcoNI CCTnn'n_nnAGG
Cuts at: 0 763 1596
Size: 763 833
EcoO109I rG'GnC_Cy
Cuts at: 0 49 475 1281 1596
Size: 49 426 806 315
Fragments arranged by size:
806 426 315 49
FspI TGC'GCA
Cuts at: 0 259 434 1181 1596
Size: 259 175 747 415
Fragments arranged by size:
747 415 259 175
HaeII r_GCGC'y
Cuts at: 0 41 123 130 305 391 1596
Size: 41 82 7 175 86 1205
Fragments arranged by size:
1205 175 86 82 41 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1133 1165 1501 1533 1596
Size: 1133 32 336 32 63
Fragments arranged by size:
1133 336 63 32 32
KasI G'GCGC_C
Cuts at: 0 119 301 1596
Size: 119 182 1295
Fragments arranged by size:
1295 182 119
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 810 1233 1596
Size: 810 423 363
Fragments arranged by size:
810 423 363
MscI TGG'CCA
Cuts at: 0 523 1558 1596
Size: 523 1035 38
Fragments arranged by size:
1035 523 38
MslI CAynn'nnrTG
Cuts at: 0 862 1228 1378 1381 1596
Size: 862 366 150 3 215
Fragments arranged by size:
862 366 215 150 3
MspA1I CmG'CkG
Cuts at: 0 82 276 323 1168 1273 1546 1596
Size: 82 194 47 845 105 273 50
Fragments arranged by size:
845 273 194 105 82 50 47
NarI GG'CG_CC
Cuts at: 0 120 302 1596
Size: 120 182 1294
Fragments arranged by size:
1294 182 120
NspI r_CATG'y
Cuts at: 0 255 1596
Size: 255 1341
PasI CC'CwG_GG
Cuts at: 0 1404 1596
Size: 1404 192
PflMI CCAn_nnn'nTGG
Cuts at: 0 129 1161 1596
Size: 129 1032 435
Fragments arranged by size:
1032 435 129
PfoI T'CCnGG_A
Cuts at: 0 787 1301 1596
Size: 787 514 295
Fragments arranged by size:
787 514 295
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 592 624 917 949 1596
Size: 592 32 293 32 647
Fragments arranged by size:
647 592 293 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 1281 1596
Size: 1281 315
PsiI TTA'TAA
Cuts at: 0 584 1596
Size: 584 1012
PspOMI G'GGCC_C
Cuts at: 0 61 1596
Size: 61 1535
PstI C_TGCA'G
Cuts at: 0 289 1596
Size: 289 1307
PvuII CAG'CTG
Cuts at: 0 323 1273 1596
Size: 323 950 323
Fragments arranged by size:
950 323 323
SacI G_AGCT'C
Cuts at: 0 72 1202 1596
Size: 72 1130 394
Fragments arranged by size:
1130 394 72
SacII CC_GC'GG
Cuts at: 0 83 1169 1596
Size: 83 1086 427
Fragments arranged by size:
1086 427 83
SapI GCTCTTCn'nnn_
Cuts at: 0 1 1596
Size: 1 1595
SfcI C'TryA_G
Cuts at: 0 285 1596
Size: 285 1311
SfoI GGC'GCC
Cuts at: 0 121 303 1596
Size: 121 182 1293
Fragments arranged by size:
1293 182 121
SnaBI TAC'GTA
Cuts at: 0 569 804 1596
Size: 569 235 792
Fragments arranged by size:
792 569 235
SphI G_CATG'C
Cuts at: 0 255 1596
Size: 255 1341
StyI C'CwwG_G
Cuts at: 0 401 1580 1596
Size: 401 1179 16
Fragments arranged by size:
1179 401 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1396 1596
Size: 1396 200
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 498 919 1131 1543 1596
Size: 498 421 212 412 53
Fragments arranged by size:
498 421 412 212 53
Enzymes that do cut:
AcuI AfeI AleI AlfI AloI ApaI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BclI BlpI
Bme1580I BplI BpmI Bpu10I BsaI BsaAI BsaHI BsaWI
BsaXI BseRI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI
Bsp1286I BspHI BspMI BsrDI BstEII BstXI BstYI BtgI
BtgZI DraIII DrdI EaeI EagI EarI EcoICRI Eco57MI
EcoNI EcoO109I FspI HaeII Hin4I KasI MmeI MscI
MslI MspA1I NarI NspI PasI PflMI PfoI PpiI
PpuMI PsiI PspOMI PstI PvuII SacI SacII SapI
SfcI SfoI SnaBI SphI StyI TaqII XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AlwNI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BciVI BfrBI BglI BglII BmgBI BmrI BmtI
BpuEI BsaBI BseYI BspEI BsrBI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstZ17I Bsu36I BtsI ClaI CspCI
DraI EciI EcoRI EcoRV FalI FseI FspAI HincII
HindIII HpaI KpnI MfeI MluI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PciI PmeI
PmlI PshAI PspXI PsrI PvuI RsrII SalI SanDI
SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SmlI
SpeI SrfI SspI StuI SwaI TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI