Restriction map for
pFN21AE4506
(Linear) (Six-base) MAPSORT of: KIEE4506.seq Check: 4198 from: 1 to: 1587
KIEE4506.orf
With 182 enzymes: SgfI *
July 3, 2012 19:00 ..
AatII G_ACGT'C
Cuts at: 0 440 1587
Size: 440 1147
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2 158 1587
Size: 2 156 1429
Fragments arranged by size:
1429 156 2
ApoI r'AATT_y
Cuts at: 0 224 261 403 786 1340 1393 1587
Size: 224 37 142 383 554 53 194
Fragments arranged by size:
554 383 224 194 142 53 37
AseI AT'TA_AT
Cuts at: 0 1298 1587
Size: 1298 289
BamHI G'GATC_C
Cuts at: 0 1160 1587
Size: 1160 427
BanII G_rGCy'C
Cuts at: 0 895 1587
Size: 895 692
BbsI GAAGACnn'nnnn_
Cuts at: 0 729 884 1374 1587
Size: 729 155 490 213
Fragments arranged by size:
729 490 213 155
BciVI GTATCCnnnnn_n'
Cuts at: 0 1061 1587
Size: 1061 526
BclI T'GATC_A
Cuts at: 0 606 1350 1587
Size: 606 744 237
Fragments arranged by size:
744 606 237
BfrBI ATG'CAT
Cuts at: 0 1282 1587
Size: 1282 305
BlpI GC'TnA_GC
Cuts at: 0 482 1587
Size: 482 1105
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 917 1587
Size: 917 670
BsaI GGTCTCn'nnnn_
Cuts at: 0 1048 1587
Size: 1048 539
BsaAI yAC'GTr
Cuts at: 0 579 1587
Size: 579 1008
BsaBI GATnn'nnATC
Cuts at: 0 1516 1587
Size: 1516 71
BsaHI Gr'CG_yC
Cuts at: 0 437 1587
Size: 437 1150
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1220 1250 1587
Size: 1220 30 337
Fragments arranged by size:
1220 337 30
BsiHKAI G_wGCw'C
Cuts at: 0 895 1587
Size: 895 692
BsmI GAATG_Cn'
Cuts at: 0 1284 1587
Size: 1284 303
Bsp1286I G_dGCh'C
Cuts at: 0 895 1587
Size: 895 692
BspHI T'CATG_A
Cuts at: 0 150 1587
Size: 150 1437
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 858 1587
Size: 858 729
BsrBI CCG'CTC
Cuts at: 0 211 1587
Size: 211 1376
BsrDI GCAATG_nn'
Cuts at: 0 1205 1587
Size: 1205 382
BsrGI T'GTAC_A
Cuts at: 0 506 885 1587
Size: 506 379 702
Fragments arranged by size:
702 506 379
BstBI TT'CG_AA
Cuts at: 0 671 774 1587
Size: 671 103 813
Fragments arranged by size:
813 671 103
BstYI r'GATC_y
Cuts at: 0 989 1160 1587
Size: 989 171 427
Fragments arranged by size:
989 427 171
Bsu36I CC'TnA_GG
Cuts at: 0 866 1038 1587
Size: 866 172 549
Fragments arranged by size:
866 549 172
BtgI C'CryG_G
Cuts at: 0 1155 1587
Size: 1155 432
BtsI GCAGTG_nn'
Cuts at: 0 840 1587
Size: 840 747
EarI CTCTTCn'nnn_
Cuts at: 0 1282 1587
Size: 1282 305
EcoICRI GAG'CTC
Cuts at: 0 893 1587
Size: 893 694
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2 158 917 1587
Size: 2 156 759 670
Fragments arranged by size:
759 670 156 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 865 1587
Size: 865 722
EcoRI G'AATT_C
Cuts at: 0 1340 1587
Size: 1340 247
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 422 454 1587
Size: 422 32 1133
Fragments arranged by size:
1133 422 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 236 810 1587
Size: 236 574 777
Fragments arranged by size:
777 574 236
MspA1I CmG'CkG
Cuts at: 0 1234 1587
Size: 1234 353
NcoI C'CATG_G
Cuts at: 0 1155 1587
Size: 1155 432
NsiI A_TGCA'T
Cuts at: 0 1284 1587
Size: 1284 303
PflMI CCAn_nnn'nTGG
Cuts at: 0 1470 1587
Size: 1470 117
PfoI T'CCnGG_A
Cuts at: 0 125 781 1587
Size: 125 656 806
Fragments arranged by size:
806 656 125
PsiI TTA'TAA
Cuts at: 0 1114 1587
Size: 1114 473
PvuII CAG'CTG
Cuts at: 0 1234 1587
Size: 1234 353
SacI G_AGCT'C
Cuts at: 0 895 1587
Size: 895 692
SexAI A'CCwGG_T
Cuts at: 0 1450 1587
Size: 1450 137
SfcI C'TryA_G
Cuts at: 0 465 1587
Size: 465 1122
SnaBI TAC'GTA
Cuts at: 0 579 1587
Size: 579 1008
SspI AAT'ATT
Cuts at: 0 353 677 1587
Size: 353 324 910
Fragments arranged by size:
910 353 324
StyI C'CwwG_G
Cuts at: 0 1155 1587
Size: 1155 432
TatI w'GTAC_w
Cuts at: 0 162 333 417 506 885 1264 1587
Size: 162 171 84 89 379 379 323
Fragments arranged by size:
379 379 323 171 162 89 84
ZraI GAC'GTC
Cuts at: 0 438 1587
Size: 438 1149
Enzymes that do cut:
AatII AcuI ApoI AseI BamHI BanII BbsI BciVI
BclI BfrBI BlpI BpmI BsaI BsaAI BsaBI BsaHI
BsaXI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrGI BstBI BstYI Bsu36I BtgI BtsI EarI EcoICRI
Eco57MI EcoNI EcoRI Hin4I MmeI MspA1I NcoI NsiI
PflMI PfoI PsiI PvuII SacI SexAI SfcI SnaBI
SspI StyI TatI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI
AsiSI AvaI AvrII BaeI BanI BbeI BbvCI BcgI
BglI BglII Bme1580I BmgBI BmrI BmtI BplI Bpu10I
BpuEI BsaWI BseRI BseYI BsgI BsiEI BsiWI BsmBI
BspEI BsrFI BssHII BssSI BstAPI BstEII BstXI BstZ17I
BtgZI ClaI CspCI DraI DraIII DrdI EaeI EagI
EciI EcoO109I EcoRV FalI FseI FspI FspAI HaeII
HincII HindIII HpaI KasI KpnI MfeI MluI MscI
MslI NaeI NarI NdeI NgoMIV NheI NotI NruI
NspI PacI PasI PciI PmeI PmlI PpiI PpuMI
PshAI PspOMI PspXI PsrI PstI PvuI RsrII SacII
SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI
SgrAI SmaI SmlI SpeI SphI SrfI StuI SwaI
TaqII TstI Tth111I XbaI XcmI XhoI XmaI XmnI