Restriction map for
pFN21AE4504
(Linear) (Six-base) MAPSORT of: KIEE4504.seq Check: 2104 from: 1 to: 1584
KIEE4504.orf
With 182 enzymes: SgfI *
March 22, 2012 18:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 453 1584
Size: 453 1131
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 41 100 112 659 754 946 1584
Size: 41 59 12 547 95 192 638
Fragments arranged by size:
638 547 192 95 59 41 12
AfeI AGC'GCT
Cuts at: 0 1014 1584
Size: 1014 570
AflII C'TTAA_G
Cuts at: 0 811 1584
Size: 811 773
AflIII A'CryG_T
Cuts at: 0 144 1397 1584
Size: 144 1253 187
Fragments arranged by size:
1253 187 144
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 343 1163 1584
Size: 343 820 421
Fragments arranged by size:
820 421 343
AlwNI CAG_nnn'CTG
Cuts at: 0 1381 1584
Size: 1381 203
ApoI r'AATT_y
Cuts at: 0 110 930 1068 1487 1584
Size: 110 820 138 419 97
Fragments arranged by size:
820 419 138 110 97
BamHI G'GATC_C
Cuts at: 0 963 1584
Size: 963 621
BanII G_rGCy'C
Cuts at: 0 673 1442 1546 1584
Size: 673 769 104 38
Fragments arranged by size:
769 673 104 38
BbsI GAAGACnn'nnnn_
Cuts at: 0 1452 1584
Size: 1452 132
BbvCI CC'TCA_GC
Cuts at: 0 449 902 1584
Size: 449 453 682
Fragments arranged by size:
682 453 449
BclI T'GATC_A
Cuts at: 0 299 558 1584
Size: 299 259 1026
Fragments arranged by size:
1026 299 259
BlpI GC'TnA_GC
Cuts at: 0 373 1584
Size: 373 1211
Bme1580I G_kGCm'C
Cuts at: 0 164 332 1232 1584
Size: 164 168 900 352
Fragments arranged by size:
900 352 168 164
BmgBI CAC'GTC
Cuts at: 0 1179 1584
Size: 1179 405
BmrI ACTGGGnnnn_n'
Cuts at: 0 126 1584
Size: 126 1458
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 334 464 938 1584
Size: 334 130 474 646
Fragments arranged by size:
646 474 334 130
Bpu10I CC'TnA_GC
Cuts at: 0 449 902 1584
Size: 449 453 682
Fragments arranged by size:
682 453 449
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 328 1426 1584
Size: 328 1098 158
Fragments arranged by size:
1098 328 158
BsaI GGTCTCn'nnnn_
Cuts at: 0 914 1584
Size: 914 670
BsaAI yAC'GTr
Cuts at: 0 876 1398 1584
Size: 876 522 186
Fragments arranged by size:
876 522 186
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 391 421 437 467 1584
Size: 391 30 16 30 1117
Fragments arranged by size:
1117 391 30 30 16
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 399 697 1096 1310 1450 1510 1584
Size: 399 298 399 214 140 60 74
Fragments arranged by size:
399 399 298 214 140 74 60
BseYI C'CCAG_C
Cuts at: 0 13 1539 1584
Size: 13 1526 45
Fragments arranged by size:
1526 45 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 16 584 1584
Size: 16 568 1000
Fragments arranged by size:
1000 568 16
BsiHKAI G_wGCw'C
Cuts at: 0 473 673 1397 1442 1584
Size: 473 200 724 45 142
Fragments arranged by size:
724 473 200 142 45
Bsp1286I G_dGCh'C
Cuts at: 0 164 332 473 673 1232 1397 1442 1546
Size: 164 168 141 200 559 165 45 104
Cuts at: 1546 1584
Size: 38
Fragments arranged by size:
559 200 168 165 164 141 104 45
38
BspHI T'CATG_A
Cuts at: 0 1529 1584
Size: 1529 55
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 59 453 1584
Size: 59 394 1131
Fragments arranged by size:
1131 394 59
BsrBI CCG'CTC
Cuts at: 0 99 1211 1584
Size: 99 1112 373
Fragments arranged by size:
1112 373 99
BsrDI GCAATG_nn'
Cuts at: 0 314 1584
Size: 314 1270
BsrGI T'GTAC_A
Cuts at: 0 398 909 1584
Size: 398 511 675
Fragments arranged by size:
675 511 398
BstAPI GCAn_nnn'nTGC
Cuts at: 0 477 1381 1584
Size: 477 904 203
Fragments arranged by size:
904 477 203
BstYI r'GATC_y
Cuts at: 0 963 1026 1584
Size: 963 63 558
Fragments arranged by size:
963 558 63
BtgI C'CryG_G
Cuts at: 0 131 256 413 1195 1584
Size: 131 125 157 782 389
Fragments arranged by size:
782 389 157 131 125
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1488 1584
Size: 1488 96
DraI TTT'AAA
Cuts at: 0 530 1584
Size: 530 1054
EaeI y'GGCC_r
Cuts at: 0 1007 1049 1584
Size: 1007 42 535
Fragments arranged by size:
1007 535 42
EarI CTCTTCn'nnn_
Cuts at: 0 17 65 515 674 678 1584
Size: 17 48 450 159 4 906
Fragments arranged by size:
906 450 159 48 17 4
EcoICRI GAG'CTC
Cuts at: 0 671 1440 1584
Size: 671 769 144
Fragments arranged by size:
769 671 144
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 41 100 112 334 464 659 754 938
Size: 41 59 12 222 130 195 95 184
Cuts at: 938 946 1584
Size: 8 638
Fragments arranged by size:
638 222 195 184 130 95 59 41
12 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 55 886 1373 1584
Size: 55 831 487 211
Fragments arranged by size:
831 487 211 55
EcoO109I rG'GnC_Cy
Cuts at: 0 1057 1584
Size: 1057 527
EcoRI G'AATT_C
Cuts at: 0 930 1068 1584
Size: 930 138 516
Fragments arranged by size:
930 516 138
EcoRV GAT'ATC
Cuts at: 0 1107 1584
Size: 1107 477
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 759 1333 1476 1584
Size: 759 574 143 108
Fragments arranged by size:
759 574 143 108
HaeII r_GCGC'y
Cuts at: 0 1016 1584
Size: 1016 568
MscI TGG'CCA
Cuts at: 0 1009 1051 1584
Size: 1009 42 533
Fragments arranged by size:
1009 533 42
MslI CAynn'nnrTG
Cuts at: 0 325 1129 1145 1584
Size: 325 804 16 439
Fragments arranged by size:
804 439 325 16
NcoI C'CATG_G
Cuts at: 0 256 1584
Size: 256 1328
NspI r_CATG'y
Cuts at: 0 148 799 1317 1584
Size: 148 651 518 267
Fragments arranged by size:
651 518 267 148
PciI A'CATG_T
Cuts at: 0 144 1584
Size: 144 1440
PfoI T'CCnGG_A
Cuts at: 0 645 915 971 1584
Size: 645 270 56 613
Fragments arranged by size:
645 613 270 56
PmlI CAC'GTG
Cuts at: 0 1398 1584
Size: 1398 186
PstI C_TGCA'G
Cuts at: 0 56 509 1247 1584
Size: 56 453 738 337
Fragments arranged by size:
738 453 337 56
SacI G_AGCT'C
Cuts at: 0 673 1442 1584
Size: 673 769 142
Fragments arranged by size:
769 673 142
SapI GCTCTTCn'nnn_
Cuts at: 0 678 1584
Size: 678 906
SbfI CC_TGCA'GG
Cuts at: 0 56 1584
Size: 56 1528
SfcI C'TryA_G
Cuts at: 0 52 505 1243 1331 1584
Size: 52 453 738 88 253
Fragments arranged by size:
738 453 253 88 52
SmlI C'TyrA_G
Cuts at: 0 343 811 1441 1584
Size: 343 468 630 143
Fragments arranged by size:
630 468 343 143
SphI G_CATG'C
Cuts at: 0 1317 1584
Size: 1317 267
StuI AGG'CCT
Cuts at: 0 742 1584
Size: 742 842
StyI C'CwwG_G
Cuts at: 0 256 332 1232 1308 1584
Size: 256 76 900 76 276
Fragments arranged by size:
900 276 256 76 76
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1157 1584
Size: 1157 427
TatI w'GTAC_w
Cuts at: 0 383 398 909 1584
Size: 383 15 511 675
Fragments arranged by size:
675 511 383 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 437 469 1584
Size: 437 32 1115
Fragments arranged by size:
1115 437 32
XbaI T'CTAG_A
Cuts at: 0 267 1584
Size: 267 1317
XmnI GAAnn'nnTTC
Cuts at: 0 742 1459 1584
Size: 742 717 125
Fragments arranged by size:
742 717 125
Enzymes that do cut:
AarI AcuI AfeI AflII AflIII AlfI AlwNI ApoI
BamHI BanII BbsI BbvCI BclI BlpI Bme1580I BmgBI
BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaXI BseRI
BseYI BsgI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrGI BstAPI BstYI BtgI BtgZI DraI EaeI EarI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII
MscI MslI NcoI NspI PciI PfoI PmlI PstI
SacI SapI SbfI SfcI SmlI SphI StuI StyI
TaqII TatI TstI XbaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AgeI AhdI AleI AloI
ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI
BanI BbeI BcgI BciVI BfrBI BglI BglII BmtI
BplI BsaBI BsaHI BsaWI BsiEI BsiWI BsmI BsmBI
BspEI BsrFI BssHII BssSI BstBI BstEII BstXI BstZ17I
Bsu36I BtsI ClaI CspCI DraIII DrdI EagI EciI
FseI FspI FspAI Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MspA1I NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI PvuII RsrII SacII SalI SanDI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
SspI SwaI Tth111I XcmI XhoI XmaI ZraI