Restriction map for
pFN21AE4501
(Linear) (Six-base) MAPSORT of: KIEE4501.seq Check: 3380 from: 1 to: 1581
KIEE4501.orf
With 182 enzymes: SgfI *
March 22, 2012 18:12 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 831 1525 1581
Size: 831 694 56
Fragments arranged by size:
831 694 56
AflIII A'CryG_T
Cuts at: 0 35 1581
Size: 35 1546
AlwNI CAG_nnn'CTG
Cuts at: 0 1309 1534 1581
Size: 1309 225 47
Fragments arranged by size:
1309 225 47
AseI AT'TA_AT
Cuts at: 0 809 1581
Size: 809 772
AvaI C'yCGr_G
Cuts at: 0 131 1387 1581
Size: 131 1256 194
Fragments arranged by size:
1256 194 131
BamHI G'GATC_C
Cuts at: 0 1144 1581
Size: 1144 437
BanI G'GyrC_C
Cuts at: 0 328 607 1581
Size: 328 279 974
Fragments arranged by size:
974 328 279
BanII G_rGCy'C
Cuts at: 0 479 1127 1581
Size: 479 648 454
Fragments arranged by size:
648 479 454
BbeI G_GCGC'C
Cuts at: 0 332 1581
Size: 332 1249
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 550 584 1581
Size: 550 34 997
Fragments arranged by size:
997 550 34
BclI T'GATC_A
Cuts at: 0 971 1581
Size: 971 610
BlpI GC'TnA_GC
Cuts at: 0 1085 1581
Size: 1085 496
Bme1580I G_kGCm'C
Cuts at: 0 610 1303 1581
Size: 610 693 278
Fragments arranged by size:
693 610 278
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1221 1581
Size: 1221 360
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1373 1581
Size: 1373 208
BsaI GGTCTCn'nnnn_
Cuts at: 0 698 1390 1581
Size: 698 692 191
Fragments arranged by size:
698 692 191
BsaAI yAC'GTr
Cuts at: 0 186 804 1581
Size: 186 618 777
Fragments arranged by size:
777 618 186
BsaHI Gr'CG_yC
Cuts at: 0 329 1581
Size: 329 1252
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 191 221 695 725 1581
Size: 191 30 474 30 856
Fragments arranged by size:
856 474 191 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 688 1210 1581
Size: 688 522 371
Fragments arranged by size:
688 522 371
BseYI C'CCAG_C
Cuts at: 0 249 1140 1581
Size: 249 891 441
Fragments arranged by size:
891 441 249
BsiHKAI G_wGCw'C
Cuts at: 0 479 1127 1581
Size: 479 648 454
Fragments arranged by size:
648 479 454
BsmBI CGTCTCn'nnnn_
Cuts at: 0 292 763 1581
Size: 292 471 818
Fragments arranged by size:
818 471 292
Bsp1286I G_dGCh'C
Cuts at: 0 479 610 1127 1303 1581
Size: 479 131 517 176 278
Fragments arranged by size:
517 479 278 176 131
BspHI T'CATG_A
Cuts at: 0 1244 1581
Size: 1244 337
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1490 1581
Size: 1490 91
BsrBI CCG'CTC
Cuts at: 0 1325 1581
Size: 1325 256
BsrDI GCAATG_nn'
Cuts at: 0 353 1581
Size: 353 1228
BsrGI T'GTAC_A
Cuts at: 0 1553 1581
Size: 1553 28
BstEII G'GTnAC_C
Cuts at: 0 1395 1581
Size: 1395 186
BstXI CCAn_nnnn'nTGG
Cuts at: 0 344 1140 1581
Size: 344 796 441
Fragments arranged by size:
796 441 344
BstYI r'GATC_y
Cuts at: 0 664 1144 1581
Size: 664 480 437
Fragments arranged by size:
664 480 437
BtgI C'CryG_G
Cuts at: 0 1277 1573 1581
Size: 1277 296 8
Fragments arranged by size:
1277 296 8
BtsI GCAGTG_nn'
Cuts at: 0 604 1033 1581
Size: 604 429 548
Fragments arranged by size:
604 548 429
DraIII CAC_nnn'GTG
Cuts at: 0 564 1319 1581
Size: 564 755 262
Fragments arranged by size:
755 564 262
EaeI y'GGCC_r
Cuts at: 0 639 1581
Size: 639 942
EarI CTCTTCn'nnn_
Cuts at: 0 1172 1581
Size: 1172 409
EcoICRI GAG'CTC
Cuts at: 0 477 1125 1581
Size: 477 648 456
Fragments arranged by size:
648 477 456
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 831 1373 1525 1581
Size: 831 542 152 56
Fragments arranged by size:
831 542 152 56
EcoRV GAT'ATC
Cuts at: 0 1410 1581
Size: 1410 171
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 861 954 1581
Size: 861 93 627
Fragments arranged by size:
861 627 93
HaeII r_GCGC'y
Cuts at: 0 332 1581
Size: 332 1249
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1233 1265 1581
Size: 1233 32 316
Fragments arranged by size:
1233 316 32
HincII GTy'rAC
Cuts at: 0 741 1273 1581
Size: 741 532 308
Fragments arranged by size:
741 532 308
HindIII A'AGCT_T
Cuts at: 0 1154 1581
Size: 1154 427
KasI G'GCGC_C
Cuts at: 0 328 1581
Size: 328 1253
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 123 439 847 1581
Size: 123 316 408 734
Fragments arranged by size:
734 408 316 123
MscI TGG'CCA
Cuts at: 0 641 1581
Size: 641 940
MslI CAynn'nnrTG
Cuts at: 0 342 1581
Size: 342 1239
MspA1I CmG'CkG
Cuts at: 0 888 1447 1581
Size: 888 559 134
Fragments arranged by size:
888 559 134
NarI GG'CG_CC
Cuts at: 0 329 1581
Size: 329 1252
NspI r_CATG'y
Cuts at: 0 39 178 445 1581
Size: 39 139 267 1136
Fragments arranged by size:
1136 267 139 39
PasI CC'CwG_GG
Cuts at: 0 428 1539 1581
Size: 428 1111 42
Fragments arranged by size:
1111 428 42
PciI A'CATG_T
Cuts at: 0 35 1581
Size: 35 1546
PflMI CCAn_nnn'nTGG
Cuts at: 0 1319 1581
Size: 1319 262
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1471 1503 1581
Size: 1471 32 78
Fragments arranged by size:
1471 78 32
SacI G_AGCT'C
Cuts at: 0 479 1127 1581
Size: 479 648 454
Fragments arranged by size:
648 479 454
ScaI AGT'ACT
Cuts at: 0 1204 1581
Size: 1204 377
SfoI GGC'GCC
Cuts at: 0 330 1581
Size: 330 1251
SnaBI TAC'GTA
Cuts at: 0 186 804 1581
Size: 186 618 777
Fragments arranged by size:
777 618 186
StuI AGG'CCT
Cuts at: 0 937 1581
Size: 937 644
StyI C'CwwG_G
Cuts at: 0 1007 1581
Size: 1007 574
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1414 1474 1581
Size: 1414 60 107
Fragments arranged by size:
1414 107 60
TatI w'GTAC_w
Cuts at: 0 1202 1553 1581
Size: 1202 351 28
Fragments arranged by size:
1202 351 28
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 191 223 695 727 1581
Size: 191 32 472 32 854
Fragments arranged by size:
854 472 191 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 46 1581
Size: 46 1535
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 360 1581
Size: 360 1221
Enzymes that do cut:
AcuI AflIII AlwNI AseI AvaI BamHI BanI BanII
BbeI BcgI BclI BlpI Bme1580I BplI BpmI BsaI
BsaAI BsaHI BsaXI BseRI BseYI BsiHKAI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrGI BstEII BstXI BstYI
BtgI BtsI DraIII EaeI EarI EcoICRI Eco57MI EcoRV
FalI HaeII Hin4I HincII HindIII KasI MmeI MscI
MslI MspA1I NarI NspI PasI PciI PflMI PpiI
SacI ScaI SfoI SnaBI StuI StyI TaqII TatI
TstI Tth111I XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI
AsiSI AvrII BaeI BbsI BbvCI BciVI BfrBI BglI
BglII BmgBI BmrI BmtI Bpu10I BpuEI BsaBI BsaWI
BsgI BsiEI BsiWI BsmI BspEI BsrFI BssHII BssSI
BstAPI BstBI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DrdI EagI EciI EcoNI EcoO109I EcoRI FseI FspI
FspAI HpaI KpnI MfeI MluI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PfoI PmeI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI PvuII RsrII SacII SalI SanDI SapI SbfI
SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SpeI
SphI SrfI SspI SwaI XbaI XhoI XmaI XmnI
ZraI