(Linear) (Six-base) MAPSORT of: KIEE4496.seq  Check: 3782  from: 1  to: 1563



KIEE4496.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 18:12  ..

AccI GT'mk_AC

Cuts at:      0     779    1563 
   Size:        779     784
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1346    1442    1563 
   Size:       1346      96     121

  Fragments arranged by size: 

                1346     121      96
 
AflII C'TTAA_G

Cuts at:      0     648    1563 
   Size:        648     915
 
ApaI G_GGCC'C

Cuts at:      0     566     795    1563 
   Size:        566     229     768

  Fragments arranged by size: 

                 768     566     229
 
ApoI r'AATT_y

Cuts at:      0      39     257    1563 
   Size:         39     218    1306

  Fragments arranged by size: 

                1306     218      39
 
AseI AT'TA_AT

Cuts at:      0    1025    1563 
   Size:       1025     538
 
BanII G_rGCy'C

Cuts at:      0     566     795    1563 
   Size:        566     229     768

  Fragments arranged by size: 

                 768     566     229
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      84     179    1418    1481    1563 
   Size:         84      95    1239      63      82

  Fragments arranged by size: 

                1239      95      84      82      63
 
BclI T'GATC_A

Cuts at:      0    1443    1563 
   Size:       1443     120
 
BlpI GC'TnA_GC

Cuts at:      0     877    1563 
   Size:        877     686
 
Bme1580I G_kGCm'C

Cuts at:      0     566     795    1563 
   Size:        566     229     768

  Fragments arranged by size: 

                 768     566     229
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     561    1563 
   Size:        561    1002
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1396    1563 
   Size:       1396     167
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      19     434    1563 
   Size:         19     415    1129

  Fragments arranged by size: 

                1129     415      19
 
BsaBI GATnn'nnATC

Cuts at:      0     128     308     380    1100    1563 
   Size:        128     180      72     720     463

  Fragments arranged by size: 

                 720     463     180     128      72
 
BseYI C'CCAG_C

Cuts at:      0     993    1563 
   Size:        993     570
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     290     893    1563 
   Size:        290     603     670

  Fragments arranged by size: 

                 670     603     290
 
Bsp1286I G_dGCh'C

Cuts at:      0     566     795    1563 
   Size:        566     229     768

  Fragments arranged by size: 

                 768     566     229
 
BspHI T'CATG_A

Cuts at:      0    1401    1563 
   Size:       1401     162
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1068    1563 
   Size:       1068     495
 
BsrDI GCAATG_nn'

Cuts at:      0     422     825    1563 
   Size:        422     403     738

  Fragments arranged by size: 

                 738     422     403
 
Bsu36I CC'TnA_GG

Cuts at:      0     487    1563 
   Size:        487    1076
 
BtsI GCAGTG_nn'

Cuts at:      0     536    1043    1058    1563 
   Size:        536     507      15     505

  Fragments arranged by size: 

                 536     507     505      15
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     514     549    1563 
   Size:        514      35    1014

  Fragments arranged by size: 

                1014     514      35
 
EarI CTCTTCn'nnn_

Cuts at:      0    1461    1563 
   Size:       1461     102
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      19    1523    1563 
   Size:         19    1504      40

  Fragments arranged by size: 

                1504      40      19
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1346    1396    1442    1563 
   Size:       1346      50      46     121

  Fragments arranged by size: 

                1346     121      50      46
 
EcoO109I rG'GnC_Cy

Cuts at:      0     563     792    1563 
   Size:        563     229     771

  Fragments arranged by size: 

                 771     563     229
 
EcoRI G'AATT_C

Cuts at:      0     257    1563 
   Size:        257    1306
 
EcoRV GAT'ATC

Cuts at:      0     165    1563 
   Size:        165    1398
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     566     598    1428    1460    1563 
   Size:        566      32     830      32     103

  Fragments arranged by size: 

                 830     566     103      32      32
 
HincII GTy'rAC

Cuts at:      0     675    1563 
   Size:        675     888
 
HindIII A'AGCT_T

Cuts at:      0     289    1563 
   Size:        289    1274
 
MfeI C'AATT_G

Cuts at:      0     317    1563 
   Size:        317    1246
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1076    1248    1563 
   Size:       1076     172     315

  Fragments arranged by size: 

                1076     315     172
 
MslI CAynn'nnrTG

Cuts at:      0     512     761    1406    1563 
   Size:        512     249     645     157

  Fragments arranged by size: 

                 645     512     249     157
 
PspOMI G'GGCC_C

Cuts at:      0     562     791    1563 
   Size:        562     229     772

  Fragments arranged by size: 

                 772     562     229
 
PstI C_TGCA'G

Cuts at:      0    1065    1563 
   Size:       1065     498
 
ScaI AGT'ACT

Cuts at:      0    1292    1563 
   Size:       1292     271
 
SfcI C'TryA_G

Cuts at:      0    1061    1563 
   Size:       1061     502
 
SmlI C'TyrA_G

Cuts at:      0      34     449     648    1563 
   Size:         34     415     199     915

  Fragments arranged by size: 

                 915     415     199      34
 
SspI AAT'ATT

Cuts at:      0     344    1563 
   Size:        344    1219
 
StyI C'CwwG_G

Cuts at:      0     252    1563 
   Size:        252    1311
 
TatI w'GTAC_w

Cuts at:      0    1290    1563 
   Size:       1290     273
 
Tth111I GACn'n_nGTC

Cuts at:      0     784    1563 
   Size:        784     779
 
XbaI T'CTAG_A

Cuts at:      0     216    1563 
   Size:        216    1347
 
XmnI GAAnn'nnTTC

Cuts at:      0     716    1391    1563 
   Size:        716     675     172

  Fragments arranged by size: 

                 716     675     172
 

 Enzymes that do cut:

     AccI     AcuI    AflII     ApaI     ApoI     AseI    BanII     BbsI
     BclI     BlpI Bme1580I     BmrI     BpmI    BpuEI    BsaBI    BseYI
     BsgI Bsp1286I    BspHI    BspMI    BsrDI   Bsu36I     BtsI    CspCI
     EarI     EciI  Eco57MI EcoO109I    EcoRI    EcoRV    Hin4I   HincII
  HindIII     MfeI     MmeI     MslI   PspOMI     PstI     ScaI     SfcI
     SmlI     SspI     StyI     TatI  Tth111I     XbaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI   AflIII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI    ApaLI     AscI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI    BciVI
    BfrBI     BglI    BglII    BmgBI     BmtI     BplI   Bpu10I     BsaI
    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI    BspEI    BsrBI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I     BtgI    BtgZI
     ClaI     DraI   DraIII     DrdI     EaeI     EagI  EcoICRI    EcoNI
     FalI     FseI     FspI    FspAI    HaeII     HpaI     KasI     KpnI
     MluI     MscI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     StuI     SwaI    TaqII
     TstI     XcmI     XhoI     XmaI     ZraI