Restriction map for
pFN21AE4490
(Linear) (Six-base) MAPSORT of: KIEE4490.seq Check: 7882 from: 1 to: 1533
KIEE4490.orf
With 182 enzymes: SgfI *
March 22, 2012 18:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 510 849 1533
Size: 510 339 684
Fragments arranged by size:
684 510 339
AatII G_ACGT'C
Cuts at: 0 210 1533
Size: 210 1323
AclI AA'CG_TT
Cuts at: 0 736 1533
Size: 736 797
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 542 1533
Size: 542 991
AlwNI CAG_nnn'CTG
Cuts at: 0 567 651 1533
Size: 567 84 882
Fragments arranged by size:
882 567 84
AseI AT'TA_AT
Cuts at: 0 289 1533
Size: 289 1244
AvaI C'yCGr_G
Cuts at: 0 577 1533
Size: 577 956
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1140 1173 1533
Size: 1140 33 360
Fragments arranged by size:
1140 360 33
BamHI G'GATC_C
Cuts at: 0 953 1533
Size: 953 580
BanI G'GyrC_C
Cuts at: 0 100 1276 1533
Size: 100 1176 257
Fragments arranged by size:
1176 257 100
BanII G_rGCy'C
Cuts at: 0 843 1082 1313 1533
Size: 843 239 231 220
Fragments arranged by size:
843 239 231 220
BbeI G_GCGC'C
Cuts at: 0 104 1533
Size: 104 1429
BbsI GAAGACnn'nnnn_
Cuts at: 0 1011 1533
Size: 1011 522
BglII A'GATC_T
Cuts at: 0 485 1533
Size: 485 1048
BlpI GC'TnA_GC
Cuts at: 0 652 1533
Size: 652 881
BmrI ACTGGGnnnn_n'
Cuts at: 0 566 1533
Size: 566 967
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 847 1533
Size: 373 474 686
Fragments arranged by size:
686 474 373
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1005 1533
Size: 1005 528
BsaI GGTCTCn'nnnn_
Cuts at: 0 1047 1408 1533
Size: 1047 361 125
Fragments arranged by size:
1047 361 125
BsaHI Gr'CG_yC
Cuts at: 0 101 207 449 1533
Size: 101 106 242 1084
Fragments arranged by size:
1084 242 106 101
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 611 641 1276 1306 1533
Size: 611 30 635 30 227
Fragments arranged by size:
635 611 227 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 38 70 102 787 816 1051 1303 1533
Size: 38 32 32 685 29 235 252 230
Fragments arranged by size:
685 252 235 230 38 32 32 29
BseYI C'CCAG_C
Cuts at: 0 585 949 1533
Size: 585 364 584
Fragments arranged by size:
585 584 364
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 817 1533
Size: 817 716
BsiEI CG_ry'CG
Cuts at: 0 1255 1533
Size: 1255 278
BsiHKAI G_wGCw'C
Cuts at: 0 467 843 1043 1533
Size: 467 376 200 490
Fragments arranged by size:
490 467 376 200
BsmI GAATG_Cn'
Cuts at: 0 153 1166 1533
Size: 153 1013 367
Fragments arranged by size:
1013 367 153
Bsp1286I G_dGCh'C
Cuts at: 0 467 843 1043 1082 1313 1533
Size: 467 376 200 39 231 220
Fragments arranged by size:
467 376 231 220 200 39
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 510 695 849 1533
Size: 510 185 154 684
Fragments arranged by size:
684 510 185 154
BsrDI GCAATG_nn'
Cuts at: 0 367 1268 1533
Size: 367 901 265
Fragments arranged by size:
901 367 265
BsrFI r'CCGG_y
Cuts at: 0 1254 1533
Size: 1254 279
BssSI C'ACGA_G
Cuts at: 0 82 1533
Size: 82 1451
BstAPI GCAn_nnn'nTGC
Cuts at: 0 333 361 1533
Size: 333 28 1172
Fragments arranged by size:
1172 333 28
BstEII G'GTnAC_C
Cuts at: 0 864 1533
Size: 864 669
BstXI CCAn_nnnn'nTGG
Cuts at: 0 901 1533
Size: 901 632
BstYI r'GATC_y
Cuts at: 0 485 500 953 968 1533
Size: 485 15 453 15 565
Fragments arranged by size:
565 485 453 15 15
BtgI C'CryG_G
Cuts at: 0 504 881 1533
Size: 504 377 652
Fragments arranged by size:
652 504 377
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1164 1533
Size: 1164 369
BtsI GCAGTG_nn'
Cuts at: 0 1200 1533
Size: 1200 333
DrdI GACnn_nn'nnGTC
Cuts at: 0 1501 1533
Size: 1501 32
EaeI y'GGCC_r
Cuts at: 0 338 604 1172 1252 1533
Size: 338 266 568 80 281
Fragments arranged by size:
568 338 281 266 80
EagI C'GGCC_G
Cuts at: 0 1252 1533
Size: 1252 281
EarI CTCTTCn'nnn_
Cuts at: 0 1096 1321 1478 1533
Size: 1096 225 157 55
Fragments arranged by size:
1096 225 157 55
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 799 1533
Size: 799 734
EcoICRI GAG'CTC
Cuts at: 0 841 1533
Size: 841 692
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 847 1533
Size: 373 474 686
Fragments arranged by size:
686 474 373
EcoO109I rG'GnC_Cy
Cuts at: 0 777 1100 1143 1533
Size: 777 323 43 390
Fragments arranged by size:
777 390 323 43
HaeII r_GCGC'y
Cuts at: 0 104 1533
Size: 104 1429
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 413 445 1046 1078 1533
Size: 413 32 601 32 455
Fragments arranged by size:
601 455 413 32 32
HincII GTy'rAC
Cuts at: 0 1284 1533
Size: 1284 249
KasI G'GCGC_C
Cuts at: 0 100 1533
Size: 100 1433
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1319 1533
Size: 1319 214
MscI TGG'CCA
Cuts at: 0 340 606 1174 1533
Size: 340 266 568 359
Fragments arranged by size:
568 359 340 266
MslI CAynn'nnrTG
Cuts at: 0 547 1533
Size: 547 986
MspA1I CmG'CkG
Cuts at: 0 648 854 1251 1501 1533
Size: 648 206 397 250 32
Fragments arranged by size:
648 397 250 206 32
NaeI GCC'GGC
Cuts at: 0 1256 1533
Size: 1256 277
NarI GG'CG_CC
Cuts at: 0 101 1533
Size: 101 1432
NcoI C'CATG_G
Cuts at: 0 881 1533
Size: 881 652
NgoMIV G'CCGG_C
Cuts at: 0 1254 1533
Size: 1254 279
PasI CC'CwG_GG
Cuts at: 0 1139 1533
Size: 1139 394
PfoI T'CCnGG_A
Cuts at: 0 668 1533
Size: 668 865
PpuMI rG'GwC_Cy
Cuts at: 0 777 1100 1143 1533
Size: 777 323 43 390
Fragments arranged by size:
777 390 323 43
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1456 1488 1533
Size: 1456 32 45
Fragments arranged by size:
1456 45 32
PstI C_TGCA'G
Cuts at: 0 1127 1195 1533
Size: 1127 68 338
Fragments arranged by size:
1127 338 68
PvuII CAG'CTG
Cuts at: 0 648 1501 1533
Size: 648 853 32
Fragments arranged by size:
853 648 32
SacI G_AGCT'C
Cuts at: 0 843 1533
Size: 843 690
SanDI GG'GwC_CC
Cuts at: 0 1100 1143 1533
Size: 1100 43 390
Fragments arranged by size:
1100 390 43
SbfI CC_TGCA'GG
Cuts at: 0 1127 1533
Size: 1127 406
SfcI C'TryA_G
Cuts at: 0 1123 1191 1533
Size: 1123 68 342
Fragments arranged by size:
1123 342 68
SfoI GGC'GCC
Cuts at: 0 102 1533
Size: 102 1431
SmlI C'TyrA_G
Cuts at: 0 984 1533
Size: 984 549
StuI AGG'CCT
Cuts at: 0 172 1530 1533
Size: 172 1358 3
Fragments arranged by size:
1358 172 3
StyI C'CwwG_G
Cuts at: 0 494 881 1533
Size: 494 387 652
Fragments arranged by size:
652 494 387
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 35 507 1533
Size: 35 472 1026
Fragments arranged by size:
1026 472 35
TatI w'GTAC_w
Cuts at: 0 988 1533
Size: 988 545
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 611 643 1533
Size: 611 32 890
Fragments arranged by size:
890 611 32
Tth111I GACn'n_nGTC
Cuts at: 0 252 1533
Size: 252 1281
XmnI GAAnn'nnTTC
Cuts at: 0 727 1533
Size: 727 806
ZraI GAC'GTC
Cuts at: 0 208 1533
Size: 208 1325
Enzymes that do cut:
AarI AatII AclI AlfI AlwNI AseI AvaI BaeI
BamHI BanI BanII BbeI BbsI BglII BlpI BmrI
BpmI BpuEI BsaI BsaHI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrDI BsrFI BssSI
BstAPI BstEII BstXI BstYI BtgI BtgZI BtsI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I HaeII
Hin4I HincII KasI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV PasI PfoI PpuMI PsrI PstI
PvuII SacI SanDI SbfI SfcI SfoI SmlI StuI
StyI TaqII TatI TstI Tth111I XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AcuI AfeI AflII AflIII AgeI AhdI
AleI AloI ApaI ApaLI ApoI AscI AsiSI AvrII
BbvCI BcgI BciVI BclI BfrBI BglI Bme1580I BmgBI
BmtI BplI Bpu10I BsaAI BsaBI BsaWI BsiWI BsmBI
BspEI BspHI BsrBI BsrGI BssHII BstBI BstZ17I Bsu36I
ClaI CspCI DraI DraIII EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HindIII HpaI KpnI MfeI MluI
NdeI NheI NotI NruI NsiI NspI PacI PciI
PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PvuI RsrII SacII SalI SapI ScaI SexAI SfiI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
SwaI XbaI XcmI XhoI XmaI