Restriction map for
pFN21AE4489
(Linear) (Six-base) MAPSORT of: KIEE4489.seq Check: 5785 from: 1 to: 1533
KIEE4489.orf
With 182 enzymes: SgfI *
March 22, 2012 18:11 ..
AccI GT'mk_AC
Cuts at: 0 659 957 982 1533
Size: 659 298 25 551
Fragments arranged by size:
659 551 298 25
Acc65I G'GTAC_C
Cuts at: 0 948 1533
Size: 948 585
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 290 932 1495 1533
Size: 290 642 563 38
Fragments arranged by size:
642 563 290 38
AflIII A'CryG_T
Cuts at: 0 873 977 1533
Size: 873 104 556
Fragments arranged by size:
873 556 104
AgeI A'CCGG_T
Cuts at: 0 61 363 1533
Size: 61 302 1170
Fragments arranged by size:
1170 302 61
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 103 135 1533
Size: 103 32 1398
Fragments arranged by size:
1398 103 32
AlwNI CAG_nnn'CTG
Cuts at: 0 96 151 1533
Size: 96 55 1382
Fragments arranged by size:
1382 96 55
ApoI r'AATT_y
Cuts at: 0 563 675 847 1357 1504 1533
Size: 563 112 172 510 147 29
Fragments arranged by size:
563 510 172 147 112 29
AvaI C'yCGr_G
Cuts at: 0 43 83 1514 1533
Size: 43 40 1431 19
Fragments arranged by size:
1431 43 40 19
BanI G'GyrC_C
Cuts at: 0 948 1533
Size: 948 585
BanII G_rGCy'C
Cuts at: 0 84 1533
Size: 84 1449
BbsI GAAGACnn'nnnn_
Cuts at: 0 449 1533
Size: 449 1084
BbvCI CC'TCA_GC
Cuts at: 0 1498 1533
Size: 1498 35
BglII A'GATC_T
Cuts at: 0 614 1127 1533
Size: 614 513 406
Fragments arranged by size:
614 513 406
Bme1580I G_kGCm'C
Cuts at: 0 59 1533
Size: 59 1474
BmrI ACTGGGnnnn_n'
Cuts at: 0 353 1533
Size: 353 1180
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1502 1533
Size: 1502 31
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 173 1533
Size: 173 1360
Bpu10I CC'TnA_GC
Cuts at: 0 1391 1498 1533
Size: 1391 107 35
Fragments arranged by size:
1391 107 35
BsaWI w'CCGG_w
Cuts at: 0 61 228 363 1533
Size: 61 167 135 1170
Fragments arranged by size:
1170 167 135 61
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 103 133 1533
Size: 103 30 1400
Fragments arranged by size:
1400 103 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 812 1533
Size: 812 721
BseYI C'CCAG_C
Cuts at: 0 122 583 1012 1533
Size: 122 461 429 521
Fragments arranged by size:
521 461 429 122
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1132 1533
Size: 1132 401
BsiHKAI G_wGCw'C
Cuts at: 0 84 1495 1533
Size: 84 1411 38
Fragments arranged by size:
1411 84 38
Bsp1286I G_dGCh'C
Cuts at: 0 59 84 1495 1533
Size: 59 25 1411 38
Fragments arranged by size:
1411 59 38 25
BsrDI GCAATG_nn'
Cuts at: 0 1385 1533
Size: 1385 148
BsrFI r'CCGG_y
Cuts at: 0 61 69 363 1533
Size: 61 8 294 1170
Fragments arranged by size:
1170 294 61 8
BstYI r'GATC_y
Cuts at: 0 614 1127 1533
Size: 614 513 406
Fragments arranged by size:
614 513 406
BstZ17I GTA'TAC
Cuts at: 0 983 1533
Size: 983 550
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 28 1533
Size: 28 1505
ClaI AT'CG_AT
Cuts at: 0 530 1533
Size: 530 1003
EaeI y'GGCC_r
Cuts at: 0 437 524 1533
Size: 437 87 1009
Fragments arranged by size:
1009 437 87
EarI CTCTTCn'nnn_
Cuts at: 0 938 1471 1533
Size: 938 533 62
Fragments arranged by size:
938 533 62
EcoICRI GAG'CTC
Cuts at: 0 82 1533
Size: 82 1451
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 173 290 932 1495 1533
Size: 173 117 642 563 38
Fragments arranged by size:
642 563 173 117 38
EcoO109I rG'GnC_Cy
Cuts at: 0 424 1396 1533
Size: 424 972 137
Fragments arranged by size:
972 424 137
EcoRV GAT'ATC
Cuts at: 0 456 1080 1533
Size: 456 624 453
Fragments arranged by size:
624 456 453
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 466 1345 1533
Size: 466 879 188
Fragments arranged by size:
879 466 188
HaeII r_GCGC'y
Cuts at: 0 7 97 1533
Size: 7 90 1436
Fragments arranged by size:
1436 90 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 165 197 594 626 1533
Size: 165 32 397 32 907
Fragments arranged by size:
907 397 165 32 32
HincII GTy'rAC
Cuts at: 0 30 958 1533
Size: 30 928 575
Fragments arranged by size:
928 575 30
KpnI G_GTAC'C
Cuts at: 0 952 1533
Size: 952 581
MfeI C'AATT_G
Cuts at: 0 683 1533
Size: 683 850
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 1296 1533
Size: 88 1208 237
Fragments arranged by size:
1208 237 88
MslI CAynn'nnrTG
Cuts at: 0 1247 1533
Size: 1247 286
MspA1I CmG'CkG
Cuts at: 0 844 1146 1533
Size: 844 302 387
Fragments arranged by size:
844 387 302
NaeI GCC'GGC
Cuts at: 0 71 1533
Size: 71 1462
NgoMIV G'CCGG_C
Cuts at: 0 69 1533
Size: 69 1464
NspI r_CATG'y
Cuts at: 0 877 981 1533
Size: 877 104 552
Fragments arranged by size:
877 552 104
PciI A'CATG_T
Cuts at: 0 873 977 1533
Size: 873 104 556
Fragments arranged by size:
873 556 104
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 103 135 1533
Size: 103 32 1398
Fragments arranged by size:
1398 103 32
PpuMI rG'GwC_Cy
Cuts at: 0 424 1396 1533
Size: 424 972 137
Fragments arranged by size:
972 424 137
PshAI GACnn'nnGTC
Cuts at: 0 422 1533
Size: 422 1111
PstI C_TGCA'G
Cuts at: 0 1367 1533
Size: 1367 166
PvuII CAG'CTG
Cuts at: 0 844 1146 1533
Size: 844 302 387
Fragments arranged by size:
844 387 302
SacI G_AGCT'C
Cuts at: 0 84 1533
Size: 84 1449
SalI G'TCGA_C
Cuts at: 0 956 1533
Size: 956 577
SexAI A'CCwGG_T
Cuts at: 0 951 1533
Size: 951 582
SfcI C'TryA_G
Cuts at: 0 663 1363 1533
Size: 663 700 170
Fragments arranged by size:
700 663 170
SgrAI Cr'CCGG_yG
Cuts at: 0 61 363 1533
Size: 61 302 1170
Fragments arranged by size:
1170 302 61
SmlI C'TyrA_G
Cuts at: 0 1514 1533
Size: 1514 19
SpeI A'CTAG_T
Cuts at: 0 168 1533
Size: 168 1365
StyI C'CwwG_G
Cuts at: 0 275 1533
Size: 275 1258
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 691 1533
Size: 691 842
TatI w'GTAC_w
Cuts at: 0 432 1533
Size: 432 1101
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1277 1309 1533
Size: 1277 32 224
Fragments arranged by size:
1277 224 32
Tth111I GACn'n_nGTC
Cuts at: 0 766 1533
Size: 766 767
XhoI C'TCGA_G
Cuts at: 0 1514 1533
Size: 1514 19
XmnI GAAnn'nnTTC
Cuts at: 0 506 917 1533
Size: 506 411 616
Fragments arranged by size:
616 506 411
Enzymes that do cut:
AccI Acc65I AcuI AflIII AgeI AloI AlwNI ApoI
AvaI BanI BanII BbsI BbvCI BglII Bme1580I BmrI
BplI BpmI Bpu10I BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI Bsp1286I BsrDI BsrFI BstYI BstZ17I BtgZI ClaI
EaeI EarI EcoICRI Eco57MI EcoO109I EcoRV FalI HaeII
Hin4I HincII KpnI MfeI MmeI MslI MspA1I NaeI
NgoMIV NspI PciI PpiI PpuMI PshAI PstI PvuII
SacI SalI SexAI SfcI SgrAI SmlI SpeI StyI
TaqII TatI TstI Tth111I XhoI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AhdI AleI AlfI
ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BcgI BciVI BclI BfrBI BglI BlpI BmgBI
BmtI BpuEI BsaI BsaAI BsaBI BsaHI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrBI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI Bsu36I BtgI BtsI
CspCI DraI DraIII DrdI EagI EciI EcoNI EcoRI
FseI FspI FspAI HindIII HpaI KasI MluI MscI
NarI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmeI PmlI PsiI PspOMI PspXI
PsrI PvuI RsrII SacII SanDI SapI SbfI ScaI
SfiI SfoI SgfI SmaI SnaBI SphI SrfI SspI
StuI SwaI XbaI XcmI XmaI ZraI