Restriction map for
pFN21AE4485
(Linear) (Six-base) MAPSORT of: KIEE4485.seq Check: 9600 from: 1 to: 1503
KIEE4485.orf
With 182 enzymes: SgfI *
March 22, 2012 18:10 ..
AflIII A'CryG_T
Cuts at: 0 411 1503
Size: 411 1092
ApoI r'AATT_y
Cuts at: 0 307 971 1012 1503
Size: 307 664 41 491
Fragments arranged by size:
664 491 307 41
BanII G_rGCy'C
Cuts at: 0 1287 1389 1503
Size: 1287 102 114
Fragments arranged by size:
1287 114 102
BbsI GAAGACnn'nnnn_
Cuts at: 0 576 830 1100 1503
Size: 576 254 270 403
Fragments arranged by size:
576 403 270 254
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1438 1472 1503
Size: 1438 34 31
Fragments arranged by size:
1438 34 31
BciVI GTATCCnnnnn_n'
Cuts at: 0 522 1503
Size: 522 981
BclI T'GATC_A
Cuts at: 0 431 1503
Size: 431 1072
BfrBI ATG'CAT
Cuts at: 0 730 1503
Size: 730 773
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 577 1094 1503
Size: 577 517 409
Fragments arranged by size:
577 517 409
Bpu10I CC'TnA_GC
Cuts at: 0 1063 1503
Size: 1063 440
BsaI GGTCTCn'nnnn_
Cuts at: 0 275 1503
Size: 275 1228
BsaAI yAC'GTr
Cuts at: 0 187 1503
Size: 187 1316
BsaBI GATnn'nnATC
Cuts at: 0 1178 1503
Size: 1178 325
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 683 713 1503
Size: 683 30 790
Fragments arranged by size:
790 683 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 704 1277 1382 1503
Size: 704 573 105 121
Fragments arranged by size:
704 573 121 105
BseYI C'CCAG_C
Cuts at: 0 4 1503
Size: 4 1499
BsiHKAI G_wGCw'C
Cuts at: 0 1108 1287 1503
Size: 1108 179 216
Fragments arranged by size:
1108 216 179
BsmBI CGTCTCn'nnnn_
Cuts at: 0 595 1503
Size: 595 908
Bsp1286I G_dGCh'C
Cuts at: 0 1108 1287 1389 1503
Size: 1108 179 102 114
Fragments arranged by size:
1108 179 114 102
BspHI T'CATG_A
Cuts at: 0 1256 1503
Size: 1256 247
BsrBI CCG'CTC
Cuts at: 0 820 1503
Size: 820 683
BsrGI T'GTAC_A
Cuts at: 0 388 1503
Size: 388 1115
BssSI C'ACGA_G
Cuts at: 0 1478 1503
Size: 1478 25
Bsu36I CC'TnA_GG
Cuts at: 0 485 1503
Size: 485 1018
BtgI C'CryG_G
Cuts at: 0 40 926 1503
Size: 40 886 577
Fragments arranged by size:
886 577 40
DraI TTT'AAA
Cuts at: 0 720 1503
Size: 720 783
DrdI GACnn_nn'nnGTC
Cuts at: 0 547 1503
Size: 547 956
EarI CTCTTCn'nnn_
Cuts at: 0 494 504 619 1503
Size: 494 10 115 884
Fragments arranged by size:
884 494 115 10
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 76 1503
Size: 76 1427
EcoICRI GAG'CTC
Cuts at: 0 1285 1503
Size: 1285 218
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 577 1094 1503
Size: 577 517 409
Fragments arranged by size:
577 517 409
HaeII r_GCGC'y
Cuts at: 0 11 17 1503
Size: 11 6 1486
Fragments arranged by size:
1486 11 6
HincII GTy'rAC
Cuts at: 0 522 1503
Size: 522 981
HpaI GTT'AAC
Cuts at: 0 522 1503
Size: 522 981
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1262 1503
Size: 1262 241
MslI CAynn'nnrTG
Cuts at: 0 874 1400 1503
Size: 874 526 103
Fragments arranged by size:
874 526 103
MspA1I CmG'CkG
Cuts at: 0 42 465 676 1503
Size: 42 423 211 827
Fragments arranged by size:
827 423 211 42
NcoI C'CATG_G
Cuts at: 0 926 1503
Size: 926 577
NsiI A_TGCA'T
Cuts at: 0 732 1503
Size: 732 771
NspI r_CATG'y
Cuts at: 0 415 1503
Size: 415 1088
PacI TTA_AT'TAA
Cuts at: 0 632 1503
Size: 632 871
PciI A'CATG_T
Cuts at: 0 411 1503
Size: 411 1092
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 119 151 1503
Size: 119 32 1352
Fragments arranged by size:
1352 119 32
PstI C_TGCA'G
Cuts at: 0 464 1503
Size: 464 1039
PvuII CAG'CTG
Cuts at: 0 465 676 1503
Size: 465 211 827
Fragments arranged by size:
827 465 211
SacI G_AGCT'C
Cuts at: 0 1287 1503
Size: 1287 216
SacII CC_GC'GG
Cuts at: 0 43 1503
Size: 43 1460
SapI GCTCTTCn'nnn_
Cuts at: 0 504 1503
Size: 504 999
SfcI C'TryA_G
Cuts at: 0 460 1503
Size: 460 1043
SpeI A'CTAG_T
Cuts at: 0 571 1503
Size: 571 932
SspI AAT'ATT
Cuts at: 0 938 1042 1274 1503
Size: 938 104 232 229
Fragments arranged by size:
938 232 229 104
StyI C'CwwG_G
Cuts at: 0 926 1503
Size: 926 577
TatI w'GTAC_w
Cuts at: 0 388 1503
Size: 388 1115
XmnI GAAnn'nnTTC
Cuts at: 0 93 793 959 1503
Size: 93 700 166 544
Fragments arranged by size:
700 544 166 93
Enzymes that do cut:
AflIII ApoI BanII BbsI BcgI BciVI BclI BfrBI
BpmI Bpu10I BsaI BsaAI BsaBI BsaXI BseRI BseYI
BsiHKAI BsmBI Bsp1286I BspHI BsrBI BsrGI BssSI Bsu36I
BtgI DraI DrdI EarI EciI EcoICRI Eco57MI HaeII
HincII HpaI MmeI MslI MspA1I NcoI NsiI NspI
PacI PciI PpiI PstI PvuII SacI SacII SapI
SfcI SpeI SspI StyI TatI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BbeI BbvCI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpuEI BsaHI BsaWI BsgI BsiEI BsiWI
BsmI BspEI BspMI BsrDI BsrFI BssHII BstAPI BstBI
BstEII BstXI BstYI BstZ17I BtgZI BtsI ClaI CspCI
DraIII EaeI EagI EcoNI EcoO109I EcoRI EcoRV FalI
FseI FspI FspAI Hin4I HindIII KasI KpnI MfeI
MluI MscI NaeI NarI NdeI NgoMIV NheI NotI
NruI PasI PflMI PfoI PmeI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SalI SanDI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SphI SrfI StuI SwaI TaqII TstI
Tth111I XbaI XcmI XhoI XmaI ZraI