Restriction map for
pFN21AE4484
(Linear) (Six-base) MAPSORT of: KIEE4484.seq Check: 4341 from: 1 to: 1500
KIEE4484.orf
With 182 enzymes: SgfI *
March 22, 2012 18:10 ..
AfeI AGC'GCT
Cuts at: 0 1167 1500
Size: 1167 333
AflIII A'CryG_T
Cuts at: 0 1241 1500
Size: 1241 259
AlwNI CAG_nnn'CTG
Cuts at: 0 756 1500
Size: 756 744
ApaI G_GGCC'C
Cuts at: 0 599 1478 1500
Size: 599 879 22
Fragments arranged by size:
879 599 22
ApoI r'AATT_y
Cuts at: 0 983 1500
Size: 983 517
AseI AT'TA_AT
Cuts at: 0 746 1500
Size: 746 754
BanI G'GyrC_C
Cuts at: 0 483 1500
Size: 483 1017
BanII G_rGCy'C
Cuts at: 0 579 599 1478 1500
Size: 579 20 879 22
Fragments arranged by size:
879 579 22 20
BbeI G_GCGC'C
Cuts at: 0 487 1500
Size: 487 1013
BbsI GAAGACnn'nnnn_
Cuts at: 0 117 711 726 1500
Size: 117 594 15 774
Fragments arranged by size:
774 594 117 15
BciVI GTATCCnnnnn_n'
Cuts at: 0 22 241 1500
Size: 22 219 1259
Fragments arranged by size:
1259 219 22
BclI T'GATC_A
Cuts at: 0 812 1500
Size: 812 688
BfrBI ATG'CAT
Cuts at: 0 103 1500
Size: 103 1397
BglII A'GATC_T
Cuts at: 0 672 693 1500
Size: 672 21 807
Fragments arranged by size:
807 672 21
BlpI GC'TnA_GC
Cuts at: 0 1108 1500
Size: 1108 392
Bme1580I G_kGCm'C
Cuts at: 0 599 1478 1500
Size: 599 879 22
Fragments arranged by size:
879 599 22
BmrI ACTGGGnnnn_n'
Cuts at: 0 1278 1500
Size: 1278 222
BmtI G_CTAG'C
Cuts at: 0 1039 1500
Size: 1039 461
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 593 1146 1433 1500
Size: 593 553 287 67
Fragments arranged by size:
593 553 287 67
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 661 1500
Size: 661 839
BsaI GGTCTCn'nnnn_
Cuts at: 0 1387 1500
Size: 1387 113
BsaHI Gr'CG_yC
Cuts at: 0 484 1280 1500
Size: 484 796 220
Fragments arranged by size:
796 484 220
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 8 38 1500
Size: 8 30 1462
Fragments arranged by size:
1462 30 8
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1498 1500
Size: 1498 2
BseYI C'CCAG_C
Cuts at: 0 50 56 1411 1500
Size: 50 6 1355 89
Fragments arranged by size:
1355 89 50 6
BsmI GAATG_Cn'
Cuts at: 0 1198 1500
Size: 1198 302
Bsp1286I G_dGCh'C
Cuts at: 0 579 599 1478 1500
Size: 579 20 879 22
Fragments arranged by size:
879 579 22 20
BspHI T'CATG_A
Cuts at: 0 263 1500
Size: 263 1237
BstEII G'GTnAC_C
Cuts at: 0 1179 1500
Size: 1179 321
BstXI CCAn_nnnn'nTGG
Cuts at: 0 195 444 1170 1500
Size: 195 249 726 330
Fragments arranged by size:
726 330 249 195
BstYI r'GATC_y
Cuts at: 0 672 693 1500
Size: 672 21 807
Fragments arranged by size:
807 672 21
BtgI C'CryG_G
Cuts at: 0 449 1500
Size: 449 1051
BtsI GCAGTG_nn'
Cuts at: 0 68 1500
Size: 68 1432
EaeI y'GGCC_r
Cuts at: 0 434 446 1500
Size: 434 12 1054
Fragments arranged by size:
1054 434 12
EarI CTCTTCn'nnn_
Cuts at: 0 859 1315 1385 1500
Size: 859 456 70 115
Fragments arranged by size:
859 456 115 70
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 593 1146 1433 1500
Size: 593 553 287 67
Fragments arranged by size:
593 553 287 67
EcoNI CCTnn'n_nnAGG
Cuts at: 0 493 1500
Size: 493 1007
EcoO109I rG'GnC_Cy
Cuts at: 0 217 1474 1500
Size: 217 1257 26
Fragments arranged by size:
1257 217 26
EcoRI G'AATT_C
Cuts at: 0 983 1500
Size: 983 517
EcoRV GAT'ATC
Cuts at: 0 182 1500
Size: 182 1318
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 655 912 1500
Size: 655 257 588
Fragments arranged by size:
655 588 257
HaeII r_GCGC'y
Cuts at: 0 487 1169 1500
Size: 487 682 331
Fragments arranged by size:
682 487 331
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 206 238 712 744 1055 1087 1500
Size: 206 32 474 32 311 32 413
Fragments arranged by size:
474 413 311 206 32 32 32
HincII GTy'rAC
Cuts at: 0 924 1500
Size: 924 576
HindIII A'AGCT_T
Cuts at: 0 528 1500
Size: 528 972
KasI G'GCGC_C
Cuts at: 0 483 1500
Size: 483 1017
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 6 1041 1125 1500
Size: 6 1035 84 375
Fragments arranged by size:
1035 375 84 6
MscI TGG'CCA
Cuts at: 0 436 448 1500
Size: 436 12 1052
Fragments arranged by size:
1052 436 12
MslI CAynn'nnrTG
Cuts at: 0 1318 1500
Size: 1318 182
NarI GG'CG_CC
Cuts at: 0 484 1500
Size: 484 1016
NcoI C'CATG_G
Cuts at: 0 449 1500
Size: 449 1051
NdeI CA'TA_TG
Cuts at: 0 1031 1500
Size: 1031 469
NheI G'CTAG_C
Cuts at: 0 1035 1500
Size: 1035 465
NsiI A_TGCA'T
Cuts at: 0 105 1500
Size: 105 1395
NspI r_CATG'y
Cuts at: 0 1245 1277 1500
Size: 1245 32 223
Fragments arranged by size:
1245 223 32
PciI A'CATG_T
Cuts at: 0 1241 1500
Size: 1241 259
PflMI CCAn_nnn'nTGG
Cuts at: 0 13 1500
Size: 13 1487
PfoI T'CCnGG_A
Cuts at: 0 755 1500
Size: 755 745
PpuMI rG'GwC_Cy
Cuts at: 0 217 1500
Size: 217 1283
PspOMI G'GGCC_C
Cuts at: 0 595 1474 1500
Size: 595 879 26
Fragments arranged by size:
879 595 26
SfoI GGC'GCC
Cuts at: 0 485 1500
Size: 485 1015
SmlI C'TyrA_G
Cuts at: 0 640 1500
Size: 640 860
StyI C'CwwG_G
Cuts at: 0 449 608 1478 1500
Size: 449 159 870 22
Fragments arranged by size:
870 449 159 22
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 790 1500
Size: 790 710
TatI w'GTAC_w
Cuts at: 0 475 1500
Size: 475 1025
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 47 1500
Size: 47 1453
Enzymes that do cut:
AfeI AflIII AlwNI ApaI ApoI AseI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BmtI BpmI BpuEI BsaI BsaHI BsaXI BseRI
BseYI BsmI Bsp1286I BspHI BstEII BstXI BstYI BtgI
BtsI EaeI EarI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI HaeII Hin4I HincII HindIII KasI MmeI MscI
MslI NarI NcoI NdeI NheI NsiI NspI PciI
PflMI PfoI PpuMI PspOMI SfoI SmlI StyI TaqII
TatI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AgeI
AhdI AleI AlfI AloI ApaLI AscI AsiSI AvaI
AvrII BaeI BamHI BbvCI BcgI BglI BmgBI BplI
Bpu10I BsaAI BsaBI BsaWI BsgI BsiEI BsiHKAI BsiWI
BsmBI BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DraIII DrdI EagI EciI EcoICRI FseI FspI
FspAI HpaI KpnI MfeI MluI MspA1I NaeI NgoMIV
NotI NruI PacI PasI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI