(Linear) (Six-base) MAPSORT of: KIEE4480.seq  Check: 433  from: 1  to: 1491



KIEE4480.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 18:09  ..

AccI GT'mk_AC

Cuts at:      0     818    1491 
   Size:        818     673
 
AclI AA'CG_TT

Cuts at:      0     585    1491 
   Size:        585     906
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     367    1242    1491 
   Size:        367     875     249

  Fragments arranged by size: 

                 875     367     249
 
AleI CACnn'nnGTG

Cuts at:      0     763    1491 
   Size:        763     728
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1155    1187    1491 
   Size:       1155      32     304

  Fragments arranged by size: 

                1155     304      32
 
ApaLI G'TGCA_C

Cuts at:      0     936    1491 
   Size:        936     555
 
AvaI C'yCGr_G

Cuts at:      0     146    1491 
   Size:        146    1345
 
BamHI G'GATC_C

Cuts at:      0     274     390    1491 
   Size:        274     116    1101

  Fragments arranged by size: 

                1101     274     116
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      68    1491 
   Size:         68    1423
 
BbvCI CC'TCA_GC

Cuts at:      0     919    1491 
   Size:        919     572
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     570    1491 
   Size:        570     921
 
BglII A'GATC_T

Cuts at:      0    1246    1491 
   Size:       1246     245
 
BlpI GC'TnA_GC

Cuts at:      0     953    1109    1491 
   Size:        953     156     382

  Fragments arranged by size: 

                 953     382     156
 
Bme1580I G_kGCm'C

Cuts at:      0     899     940    1491 
   Size:        899      41     551

  Fragments arranged by size: 

                 899     551      41
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     350    1491 
   Size:        350    1141
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     622    1491 
   Size:        622     869
 
Bpu10I CC'TnA_GC

Cuts at:      0     919    1103    1491 
   Size:        919     184     388

  Fragments arranged by size: 

                 919     388     184
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1198    1491 
   Size:       1198     293
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     644     674    1155    1185    1491 
   Size:        644      30     481      30     306

  Fragments arranged by size: 

                 644     481     306      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     475     801    1491 
   Size:        475     326     690

  Fragments arranged by size: 

                 690     475     326
 
BsiHKAI G_wGCw'C

Cuts at:      0     940    1491 
   Size:        940     551
 
BsmI GAATG_Cn'

Cuts at:      0     230    1491 
   Size:        230    1261
 
Bsp1286I G_dGCh'C

Cuts at:      0     899     940    1491 
   Size:        899      41     551

  Fragments arranged by size: 

                 899     551      41
 
BspHI T'CATG_A

Cuts at:      0     459    1491 
   Size:        459    1032
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     162     783    1185    1491 
   Size:        162     621     402     306

  Fragments arranged by size: 

                 621     402     306     162
 
BsrGI T'GTAC_A

Cuts at:      0     401     727    1491 
   Size:        401     326     764

  Fragments arranged by size: 

                 764     401     326
 
BstYI r'GATC_y

Cuts at:      0     274     390     654    1246    1491 
   Size:        274     116     264     592     245

  Fragments arranged by size: 

                 592     274     264     245     116
 
Bsu36I CC'TnA_GG

Cuts at:      0     784    1491 
   Size:        784     707
 
BtgI C'CryG_G

Cuts at:      0     572    1491 
   Size:        572     919
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     367     622    1242    1491 
   Size:        367     255     620     249

  Fragments arranged by size: 

                 620     367     255     249
 
EcoO109I rG'GnC_Cy

Cuts at:      0     138    1491 
   Size:        138    1353
 
EcoRV GAT'ATC

Cuts at:      0     696    1491 
   Size:        696     795
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     296     328     375     407    1231    1263    1491 
   Size:        296      32      47      32     824      32     228

  Fragments arranged by size: 

                 824     296     228      47      32      32      32
 
HincII GTy'rAC

Cuts at:      0     252     309    1332    1491 
   Size:        252      57    1023     159

  Fragments arranged by size: 

                1023     252     159      57
 
HindIII A'AGCT_T

Cuts at:      0    1272    1491 
   Size:       1272     219
 
HpaI GTT'AAC

Cuts at:      0     309    1332    1491 
   Size:        309    1023     159

  Fragments arranged by size: 

                1023     309     159
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     418    1248    1423    1491 
   Size:        418     830     175      68

  Fragments arranged by size: 

                 830     418     175      68
 
MslI CAynn'nnrTG

Cuts at:      0     763    1491 
   Size:        763     728
 
MspA1I CmG'CkG

Cuts at:      0     952    1491 
   Size:        952     539
 
NcoI C'CATG_G

Cuts at:      0     572    1491 
   Size:        572     919
 
NspI r_CATG'y

Cuts at:      0     764    1491 
   Size:        764     727
 
PasI CC'CwG_GG

Cuts at:      0     900    1491 
   Size:        900     591
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1155    1187    1491 
   Size:       1155      32     304

  Fragments arranged by size: 

                1155     304      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     138    1491 
   Size:        138    1353
 
SfcI C'TryA_G

Cuts at:      0     300    1491 
   Size:        300    1191
 
StyI C'CwwG_G

Cuts at:      0     572    1491 
   Size:        572     919
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      36    1491 
   Size:         36    1455
 
TatI w'GTAC_w

Cuts at:      0     401     727    1225    1491 
   Size:        401     326     498     266

  Fragments arranged by size: 

                 498     401     326     266
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     644     676    1491 
   Size:        644      32     815

  Fragments arranged by size: 

                 815     644      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     897    1491 
   Size:        897     594
 

 Enzymes that do cut:

     AccI     AclI     AcuI     AleI     AloI    ApaLI     AvaI    BamHI
     BbsI    BbvCI    BciVI    BglII     BlpI Bme1580I     BmrI     BpmI
   Bpu10I     BsaI    BsaXI    BseRI  BsiHKAI     BsmI Bsp1286I    BspHI
    BspMI    BsrGI    BstYI   Bsu36I     BtgI  Eco57MI EcoO109I    EcoRV
    Hin4I   HincII  HindIII     HpaI     MmeI     MslI   MspA1I     NcoI
     NspI     PasI     PpiI    PpuMI     SfcI     StyI    TaqII     TatI
     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AfeI    AflII   AflIII     AgeI     AhdI
     AlfI    AlwNI     ApaI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI     BanI    BanII     BbeI     BcgI     BclI    BfrBI     BglI
    BmgBI     BmtI     BplI    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI
    BseYI     BsgI    BsiEI    BsiWI    BsmBI    BspEI    BsrBI    BsrDI
    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRI     FalI     FseI
     FspI    FspAI    HaeII     KasI     KpnI     MfeI     MluI     MscI
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PciI    PflMI     PfoI     PmeI     PmlI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI