Restriction map for
pFN21AE4478
(Linear) (Six-base) MAPSORT of: KIEE4478.seq Check: 4809 from: 1 to: 1488
KIEE4478.orf
With 182 enzymes: SgfI *
March 22, 2012 18:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 886 1488
Size: 886 602
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 560 1161 1488
Size: 560 601 327
Fragments arranged by size:
601 560 327
AhdI GACnn_n'nnGTC
Cuts at: 0 250 1488
Size: 250 1238
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 295 1488
Size: 295 1193
ApaI G_GGCC'C
Cuts at: 0 1189 1488
Size: 1189 299
ApoI r'AATT_y
Cuts at: 0 118 494 523 1488
Size: 118 376 29 965
Fragments arranged by size:
965 376 118 29
BanI G'GyrC_C
Cuts at: 0 454 691 1488
Size: 454 237 797
Fragments arranged by size:
797 454 237
BanII G_rGCy'C
Cuts at: 0 779 911 1189 1488
Size: 779 132 278 299
Fragments arranged by size:
779 299 278 132
BciVI GTATCCnnnnn_n'
Cuts at: 0 714 1488
Size: 714 774
BglII A'GATC_T
Cuts at: 0 431 509 1420 1488
Size: 431 78 911 68
Fragments arranged by size:
911 431 78 68
BlpI GC'TnA_GC
Cuts at: 0 665 1488
Size: 665 823
Bme1580I G_kGCm'C
Cuts at: 0 416 694 1189 1488
Size: 416 278 495 299
Fragments arranged by size:
495 416 299 278
BmgBI CAC'GTC
Cuts at: 0 179 1488
Size: 179 1309
BmrI ACTGGGnnnn_n'
Cuts at: 0 92 571 1488
Size: 92 479 917
Fragments arranged by size:
917 479 92
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 661 811 1488
Size: 661 150 677
Fragments arranged by size:
677 661 150
Bpu10I CC'TnA_GC
Cuts at: 0 1466 1488
Size: 1466 22
BsaI GGTCTCn'nnnn_
Cuts at: 0 1348 1465 1488
Size: 1348 117 23
Fragments arranged by size:
1348 117 23
BsaHI Gr'CG_yC
Cuts at: 0 1436 1488
Size: 1436 52
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1216 1246 1274 1304 1488
Size: 1216 30 28 30 184
Fragments arranged by size:
1216 184 30 30 28
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 670 1488
Size: 670 818
BseYI C'CCAG_C
Cuts at: 0 21 477 1194 1488
Size: 21 456 717 294
Fragments arranged by size:
717 456 294 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 384 617 1433 1488
Size: 384 233 816 55
Fragments arranged by size:
816 384 233 55
BsiHKAI G_wGCw'C
Cuts at: 0 779 827 1488
Size: 779 48 661
Fragments arranged by size:
779 661 48
BsmI GAATG_Cn'
Cuts at: 0 666 1488
Size: 666 822
BsmBI CGTCTCn'nnnn_
Cuts at: 0 185 1488
Size: 185 1303
Bsp1286I G_dGCh'C
Cuts at: 0 416 694 779 827 911 1189 1488
Size: 416 278 85 48 84 278 299
Fragments arranged by size:
416 299 278 278 85 84 48
BspHI T'CATG_A
Cuts at: 0 1043 1488
Size: 1043 445
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 886 1488
Size: 886 602
BsrFI r'CCGG_y
Cuts at: 0 1213 1306 1488
Size: 1213 93 182
Fragments arranged by size:
1213 182 93
BsrGI T'GTAC_A
Cuts at: 0 419 1488
Size: 419 1069
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1411 1488
Size: 1411 77
BstEII G'GTnAC_C
Cuts at: 0 15 1488
Size: 15 1473
BstXI CCAn_nnnn'nTGG
Cuts at: 0 514 1488
Size: 514 974
BstYI r'GATC_y
Cuts at: 0 431 509 671 1420 1488
Size: 431 78 162 749 68
Fragments arranged by size:
749 431 162 78 68
BtgI C'CryG_G
Cuts at: 0 1359 1488
Size: 1359 129
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1216 1488
Size: 1216 272
BtsI GCAGTG_nn'
Cuts at: 0 1214 1488
Size: 1214 274
DraIII CAC_nnn'GTG
Cuts at: 0 1411 1488
Size: 1411 77
EaeI y'GGCC_r
Cuts at: 0 200 550 1166 1211 1488
Size: 200 350 616 45 277
Fragments arranged by size:
616 350 277 200 45
EarI CTCTTCn'nnn_
Cuts at: 0 253 391 536 647 1180 1261 1488
Size: 253 138 145 111 533 81 227
Fragments arranged by size:
533 253 227 145 138 111 81
EcoICRI GAG'CTC
Cuts at: 0 777 1488
Size: 777 711
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 560 661 811 1161 1488
Size: 560 101 150 350 327
Fragments arranged by size:
560 350 327 150 101
EcoO109I rG'GnC_Cy
Cuts at: 0 1144 1185 1186 1488
Size: 1144 41 1 302
Fragments arranged by size:
1144 302 41 1
EcoRI G'AATT_C
Cuts at: 0 494 523 1488
Size: 494 29 965
Fragments arranged by size:
965 494 29
EcoRV GAT'ATC
Cuts at: 0 1380 1488
Size: 1380 108
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 236 268 351 383 501 533 620 652
Size: 236 32 83 32 118 32 87 32
Cuts at: 652 791 823 1488
Size: 139 32 665
Fragments arranged by size:
665 236 139 118 87 83 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 255 291 1488
Size: 255 36 1197
Fragments arranged by size:
1197 255 36
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 222 471 1227 1247 1259 1488
Size: 222 249 756 20 12 229
Fragments arranged by size:
756 249 229 222 20 12
MscI TGG'CCA
Cuts at: 0 202 1488
Size: 202 1286
MslI CAynn'nnrTG
Cuts at: 0 1042 1444 1488
Size: 1042 402 44
Fragments arranged by size:
1042 402 44
MspA1I CmG'CkG
Cuts at: 0 477 555 681 1198 1267 1488
Size: 477 78 126 517 69 221
Fragments arranged by size:
517 477 221 126 78 69
NaeI GCC'GGC
Cuts at: 0 1215 1488
Size: 1215 273
NdeI CA'TA_TG
Cuts at: 0 1004 1488
Size: 1004 484
NgoMIV G'CCGG_C
Cuts at: 0 1213 1488
Size: 1213 275
PflMI CCAn_nnn'nTGG
Cuts at: 0 1154 1488
Size: 1154 334
PfoI T'CCnGG_A
Cuts at: 0 716 1488
Size: 716 772
PspOMI G'GGCC_C
Cuts at: 0 1185 1488
Size: 1185 303
PvuII CAG'CTG
Cuts at: 0 477 1198 1488
Size: 477 721 290
Fragments arranged by size:
721 477 290
SacI G_AGCT'C
Cuts at: 0 779 1488
Size: 779 709
SapI GCTCTTCn'nnn_
Cuts at: 0 536 1261 1488
Size: 536 725 227
Fragments arranged by size:
725 536 227
SfcI C'TryA_G
Cuts at: 0 956 1488
Size: 956 532
StuI AGG'CCT
Cuts at: 0 1013 1488
Size: 1013 475
StyI C'CwwG_G
Cuts at: 0 531 1014 1488
Size: 531 483 474
Fragments arranged by size:
531 483 474
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 709 1488
Size: 709 779
TatI w'GTAC_w
Cuts at: 0 419 1488
Size: 419 1069
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 485 517 1488
Size: 485 32 971
Fragments arranged by size:
971 485 32
Tth111I GACn'n_nGTC
Cuts at: 0 1435 1488
Size: 1435 53
Enzymes that do cut:
AarI AcuI AhdI AlfI ApaI ApoI BanI BanII
BciVI BglII BlpI Bme1580I BmgBI BmrI BpmI Bpu10I
BsaI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BspMI BsrFI BsrGI BstAPI BstEII
BstXI BstYI BtgI BtgZI BtsI DraIII EaeI EarI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV Hin4I HincII MmeI
MscI MslI MspA1I NaeI NdeI NgoMIV PflMI PfoI
PspOMI PvuII SacI SapI SfcI StuI StyI TaqII
TatI TstI Tth111I
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AleI AloI AlwNI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BbsI BbvCI BcgI BclI
BfrBI BglI BmtI BplI BpuEI BsaAI BsaBI BsaWI
BsiEI BsiWI BspEI BsrBI BsrDI BssHII BssSI BstBI
BstZ17I Bsu36I ClaI CspCI DraI DrdI EagI EciI
EcoNI FalI FseI FspI FspAI HaeII HindIII HpaI
KasI KpnI MfeI MluI NarI NcoI NheI NotI
NruI NsiI NspI PacI PasI PciI PmeI PmlI
PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI
RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI SwaI XbaI XcmI XhoI XmaI XmnI
ZraI