Restriction map for
pFN21AE4476
(Linear) (Six-base) MAPSORT of: KIEE4476.seq Check: 2033 from: 1 to: 1485
KIEE4476.orf
With 182 enzymes: SgfI *
March 22, 2012 18:09 ..
Acc65I G'GTAC_C
Cuts at: 0 846 1485
Size: 846 639
AflIII A'CryG_T
Cuts at: 0 168 1485
Size: 168 1317
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 321 353 1485
Size: 321 32 1132
Fragments arranged by size:
1132 321 32
ApaI G_GGCC'C
Cuts at: 0 1322 1485
Size: 1322 163
ApoI r'AATT_y
Cuts at: 0 770 1068 1094 1485
Size: 770 298 26 391
Fragments arranged by size:
770 391 298 26
AseI AT'TA_AT
Cuts at: 0 1397 1485
Size: 1397 88
BamHI G'GATC_C
Cuts at: 0 425 1485
Size: 425 1060
BanI G'GyrC_C
Cuts at: 0 73 846 1408 1485
Size: 73 773 562 77
Fragments arranged by size:
773 562 77 73
BanII G_rGCy'C
Cuts at: 0 570 1322 1458 1485
Size: 570 752 136 27
Fragments arranged by size:
752 570 136 27
BbeI G_GCGC'C
Cuts at: 0 77 1485
Size: 77 1408
BbsI GAAGACnn'nnnn_
Cuts at: 0 551 678 1485
Size: 551 127 807
Fragments arranged by size:
807 551 127
BciVI GTATCCnnnnn_n'
Cuts at: 0 1279 1485
Size: 1279 206
BclI T'GATC_A
Cuts at: 0 1464 1485
Size: 1464 21
BfrBI ATG'CAT
Cuts at: 0 163 546 1485
Size: 163 383 939
Fragments arranged by size:
939 383 163
BglI GCCn_nnn'nGGC
Cuts at: 0 82 1485
Size: 82 1403
Bme1580I G_kGCm'C
Cuts at: 0 1322 1485
Size: 1322 163
BmrI ACTGGGnnnn_n'
Cuts at: 0 1213 1461 1485
Size: 1213 248 24
Fragments arranged by size:
1213 248 24
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 80 395 1485
Size: 80 315 1090
Fragments arranged by size:
1090 315 80
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 305 1485
Size: 305 1180
BsaI GGTCTCn'nnnn_
Cuts at: 0 11 1485
Size: 11 1474
BsaAI yAC'GTr
Cuts at: 0 169 1485
Size: 169 1316
BsaHI Gr'CG_yC
Cuts at: 0 74 1485
Size: 74 1411
BsaWI w'CCGG_w
Cuts at: 0 9 1485
Size: 9 1476
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 368 398 512 542 1485
Size: 368 30 114 30 943
Fragments arranged by size:
943 368 114 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 55 1485
Size: 55 1430
BsiHKAI G_wGCw'C
Cuts at: 0 49 507 618 1485
Size: 49 458 111 867
Fragments arranged by size:
867 458 111 49
BsmI GAATG_Cn'
Cuts at: 0 255 1485
Size: 255 1230
Bsp1286I G_dGCh'C
Cuts at: 0 49 507 570 618 1322 1458 1485
Size: 49 458 63 48 704 136 27
Fragments arranged by size:
704 458 136 63 49 48 27
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1131 1485
Size: 1131 354
BsrBI CCG'CTC
Cuts at: 0 94 1485
Size: 94 1391
BsrDI GCAATG_nn'
Cuts at: 0 461 994 1325 1485
Size: 461 533 331 160
Fragments arranged by size:
533 461 331 160
BstYI r'GATC_y
Cuts at: 0 425 731 953 1485
Size: 425 306 222 532
Fragments arranged by size:
532 425 306 222
Bsu36I CC'TnA_GG
Cuts at: 0 958 1485
Size: 958 527
BtgI C'CryG_G
Cuts at: 0 79 841 1470 1485
Size: 79 762 629 15
Fragments arranged by size:
762 629 79 15
BtsI GCAGTG_nn'
Cuts at: 0 434 1485
Size: 434 1051
DraI TTT'AAA
Cuts at: 0 857 1485
Size: 857 628
EaeI y'GGCC_r
Cuts at: 0 1172 1485
Size: 1172 313
EarI CTCTTCn'nnn_
Cuts at: 0 68 644 1224 1485
Size: 68 576 580 261
Fragments arranged by size:
580 576 261 68
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 80 395 1485
Size: 80 315 1090
Fragments arranged by size:
1090 315 80
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1004 1485
Size: 1004 481
HaeII r_GCGC'y
Cuts at: 0 77 1485
Size: 77 1408
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 317 349 410 442 1217 1249 1485
Size: 317 32 61 32 775 32 236
Fragments arranged by size:
775 317 236 61 32 32 32
KasI G'GCGC_C
Cuts at: 0 73 1485
Size: 73 1412
KpnI G_GTAC'C
Cuts at: 0 850 1485
Size: 850 635
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 462 1338 1485
Size: 462 876 147
Fragments arranged by size:
876 462 147
NarI GG'CG_CC
Cuts at: 0 74 1485
Size: 74 1411
NcoI C'CATG_G
Cuts at: 0 1470 1485
Size: 1470 15
NdeI CA'TA_TG
Cuts at: 0 606 1485
Size: 606 879
NsiI A_TGCA'T
Cuts at: 0 165 548 1485
Size: 165 383 937
Fragments arranged by size:
937 383 165
NspI r_CATG'y
Cuts at: 0 248 550 1485
Size: 248 302 935
Fragments arranged by size:
935 302 248
PfoI T'CCnGG_A
Cuts at: 0 372 1305 1485
Size: 372 933 180
Fragments arranged by size:
933 372 180
PshAI GACnn'nnGTC
Cuts at: 0 682 1485
Size: 682 803
PspOMI G'GGCC_C
Cuts at: 0 1318 1485
Size: 1318 167
PstI C_TGCA'G
Cuts at: 0 471 1128 1485
Size: 471 657 357
Fragments arranged by size:
657 471 357
RsrII CG'GwC_CG
Cuts at: 0 51 1485
Size: 51 1434
SapI GCTCTTCn'nnn_
Cuts at: 0 1224 1485
Size: 1224 261
SfcI C'TryA_G
Cuts at: 0 467 1124 1485
Size: 467 657 361
Fragments arranged by size:
657 467 361
SfoI GGC'GCC
Cuts at: 0 75 1485
Size: 75 1410
SmlI C'TyrA_G
Cuts at: 0 320 1485
Size: 320 1165
SphI G_CATG'C
Cuts at: 0 550 1485
Size: 550 935
SspI AAT'ATT
Cuts at: 0 713 1485
Size: 713 772
StyI C'CwwG_G
Cuts at: 0 239 429 1470 1485
Size: 239 190 1041 15
Fragments arranged by size:
1041 239 190 15
TatI w'GTAC_w
Cuts at: 0 495 1443 1485
Size: 495 948 42
Fragments arranged by size:
948 495 42
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 354 386 1485
Size: 354 32 1099
Fragments arranged by size:
1099 354 32
XmnI GAAnn'nnTTC
Cuts at: 0 794 1485
Size: 794 691
Enzymes that do cut:
Acc65I AflIII AloI ApaI ApoI AseI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglI Bme1580I
BmrI BpmI BpuEI BsaI BsaAI BsaHI BsaWI BsaXI
BseRI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI BstYI
Bsu36I BtgI BtsI DraI EaeI EarI Eco57MI EcoNI
HaeII Hin4I KasI KpnI MmeI NarI NcoI NdeI
NsiI NspI PfoI PshAI PspOMI PstI RsrII SapI
SfcI SfoI SmlI SphI SspI StyI TatI TstI
XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AflII AgeI
AhdI AleI AlfI AlwNI ApaLI AscI AsiSI AvaI
AvrII BaeI BbvCI BcgI BglII BlpI BmgBI BmtI
BplI Bpu10I BsaBI BseYI BsgI BsiEI BsiWI BsmBI
BspEI BspHI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstZ17I BtgZI ClaI CspCI DraIII DrdI
EagI EciI EcoICRI EcoO109I EcoRI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI MfeI MluI MscI
MslI MspA1I NaeI NgoMIV NheI NotI NruI PacI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PsiI
PspXI PsrI PvuI PvuII SacI SacII SalI SanDI
SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI
SpeI SrfI StuI SwaI TaqII Tth111I XbaI XcmI
XhoI XmaI ZraI