Restriction map for
pFN21AE4474
(Linear) (Six-base) MAPSORT of: KIEE4474.seq Check: 590 from: 1 to: 1479
KIEE4474.orf
With 182 enzymes: SgfI *
February 29, 2012 11:54 ..
AatII G_ACGT'C
Cuts at: 0 1238 1479
Size: 1238 241
AccI GT'mk_AC
Cuts at: 0 518 1479
Size: 518 961
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 1429 1453 1479
Size: 139 1290 24 26
Fragments arranged by size:
1290 139 26 24
AfeI AGC'GCT
Cuts at: 0 451 553 1479
Size: 451 102 926
Fragments arranged by size:
926 451 102
AflIII A'CryG_T
Cuts at: 0 101 815 1479
Size: 101 714 664
Fragments arranged by size:
714 664 101
AhdI GACnn_n'nnGTC
Cuts at: 0 354 1479
Size: 354 1125
AleI CACnn'nnGTG
Cuts at: 0 739 1342 1479
Size: 739 603 137
Fragments arranged by size:
739 603 137
ApaI G_GGCC'C
Cuts at: 0 707 1479
Size: 707 772
ApaLI G'TGCA_C
Cuts at: 0 400 1479
Size: 400 1079
AscI GG'CGCG_CC
Cuts at: 0 610 1479
Size: 610 869
AvaI C'yCGr_G
Cuts at: 0 616 1479
Size: 616 863
BanI G'GyrC_C
Cuts at: 0 249 340 599 693 1204 1261 1479
Size: 249 91 259 94 511 57 218
Fragments arranged by size:
511 259 249 218 94 91 57
BanII G_rGCy'C
Cuts at: 0 707 758 1124 1479
Size: 707 51 366 355
Fragments arranged by size:
707 366 355 51
BbeI G_GCGC'C
Cuts at: 0 603 697 1265 1479
Size: 603 94 568 214
Fragments arranged by size:
603 568 214 94
BbsI GAAGACnn'nnnn_
Cuts at: 0 1230 1479
Size: 1230 249
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 742 776 1479
Size: 742 34 703
Fragments arranged by size:
742 703 34
BglI GCCn_nnn'nGGC
Cuts at: 0 197 702 1479
Size: 197 505 777
Fragments arranged by size:
777 505 197
BglII A'GATC_T
Cuts at: 0 131 1479
Size: 131 1348
Bme1580I G_kGCm'C
Cuts at: 0 254 404 509 707 1207 1479
Size: 254 150 105 198 500 272
Fragments arranged by size:
500 272 254 198 150 105
BmgBI CAC'GTC
Cuts at: 0 504 1479
Size: 504 975
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 666 1479
Size: 666 813
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 661 787 907 966 1209 1267 1479
Size: 163 498 126 120 59 243 58 212
Fragments arranged by size:
498 243 212 163 126 120 59 58
Bpu10I CC'TnA_GC
Cuts at: 0 463 1479
Size: 463 1016
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 703 1479
Size: 703 776
BsaI GGTCTCn'nnnn_
Cuts at: 0 856 868 1479
Size: 856 12 611
Fragments arranged by size:
856 611 12
BsaHI Gr'CG_yC
Cuts at: 0 515 600 694 1045 1214 1235 1262 1427
Size: 515 85 94 351 169 21 27 165
Cuts at: 1427 1479
Size: 52
Fragments arranged by size:
515 351 169 165 94 85 52 27
21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 874 1479
Size: 874 605
BseYI C'CCAG_C
Cuts at: 0 706 1479
Size: 706 773
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 583 988 1479
Size: 49 534 405 491
Fragments arranged by size:
534 491 405 49
BsiEI CG_ry'CG
Cuts at: 0 77 176 297 475 479 897 1479
Size: 77 99 121 178 4 418 582
Fragments arranged by size:
582 418 178 121 99 77 4
BsiHKAI G_wGCw'C
Cuts at: 0 404 758 1124 1479
Size: 404 354 366 355
Fragments arranged by size:
404 366 355 354
BsmI GAATG_Cn'
Cuts at: 0 996 1479
Size: 996 483
Bsp1286I G_dGCh'C
Cuts at: 0 254 404 509 707 758 1124 1207 1479
Size: 254 150 105 198 51 366 83 272
Fragments arranged by size:
366 272 254 198 150 105 83 51
BspHI T'CATG_A
Cuts at: 0 1130 1479
Size: 1130 349
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 244 1479
Size: 244 1235
BsrBI CCG'CTC
Cuts at: 0 650 1223 1479
Size: 650 573 256
Fragments arranged by size:
650 573 256
BsrFI r'CCGG_y
Cuts at: 0 323 474 621 1025 1479
Size: 323 151 147 404 454
Fragments arranged by size:
454 404 323 151 147
BssHII G'CGCG_C
Cuts at: 0 467 610 1479
Size: 467 143 869
Fragments arranged by size:
869 467 143
BstEII G'GTnAC_C
Cuts at: 0 1320 1479
Size: 1320 159
BstXI CCAn_nnnn'nTGG
Cuts at: 0 741 1392 1479
Size: 741 651 87
Fragments arranged by size:
741 651 87
BstYI r'GATC_y
Cuts at: 0 32 131 1479
Size: 32 99 1348
Fragments arranged by size:
1348 99 32
Bsu36I CC'TnA_GG
Cuts at: 0 419 1479
Size: 419 1060
BtgI C'CryG_G
Cuts at: 0 491 1164 1316 1451 1479
Size: 491 673 152 135 28
Fragments arranged by size:
673 491 152 135 28
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 390 1479
Size: 390 1089
BtsI GCAGTG_nn'
Cuts at: 0 1314 1479
Size: 1314 165
DrdI GACnn_nn'nnGTC
Cuts at: 0 1318 1479
Size: 1318 161
EaeI y'GGCC_r
Cuts at: 0 74 173 472 476 623 800 953 1088
Size: 74 99 299 4 147 177 153 135
Cuts at: 1088 1382 1479
Size: 294 97
Fragments arranged by size:
299 294 177 153 147 135 99 97
74 4
EagI C'GGCC_G
Cuts at: 0 74 173 472 476 1479
Size: 74 99 299 4 1003
Fragments arranged by size:
1003 299 99 74 4
EarI CTCTTCn'nnn_
Cuts at: 0 1129 1479
Size: 1129 350
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 96 229 1459 1479
Size: 96 133 1230 20
Fragments arranged by size:
1230 133 96 20
EcoICRI GAG'CTC
Cuts at: 0 756 1122 1479
Size: 756 366 357
Fragments arranged by size:
756 366 357
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 163 661 787 907 966 1209 1267
Size: 139 24 498 126 120 59 243 58
Cuts at: 1267 1429 1453 1479
Size: 162 24 26
Fragments arranged by size:
498 243 162 139 126 120 59 58
26 24 24
EcoO109I rG'GnC_Cy
Cuts at: 0 703 1479
Size: 703 776
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 681 933 1479
Size: 681 252 546
Fragments arranged by size:
681 546 252
FseI GG_CCGG'CC
Cuts at: 0 478 1479
Size: 478 1001
FspI TGC'GCA
Cuts at: 0 187 1479
Size: 187 1292
HaeII r_GCGC'y
Cuts at: 0 330 453 555 603 697 1265 1404 1479
Size: 330 123 102 48 94 568 139 75
Fragments arranged by size:
568 330 139 123 102 94 75 48
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 340 372 1447 1479
Size: 340 32 1075 32
Fragments arranged by size:
1075 340 32 32
HincII GTy'rAC
Cuts at: 0 426 519 1479
Size: 426 93 960
Fragments arranged by size:
960 426 93
KasI G'GCGC_C
Cuts at: 0 599 693 1261 1479
Size: 599 94 568 218
Fragments arranged by size:
599 568 218 94
MluI A'CGCG_T
Cuts at: 0 101 1479
Size: 101 1378
MscI TGG'CCA
Cuts at: 0 1384 1479
Size: 1384 95
MslI CAynn'nnrTG
Cuts at: 0 409 739 1156 1342 1390 1479
Size: 409 330 417 186 48 89
Fragments arranged by size:
417 409 330 186 89 48
MspA1I CmG'CkG
Cuts at: 0 710 885 998 1086 1166 1245 1318 1331
Size: 710 175 113 88 80 79 73 13
Cuts at: 1331 1479
Size: 148
Fragments arranged by size:
710 175 148 113 88 80 79 73
13
NaeI GCC'GGC
Cuts at: 0 476 623 1479
Size: 476 147 856
Fragments arranged by size:
856 476 147
NarI GG'CG_CC
Cuts at: 0 600 694 1262 1479
Size: 600 94 568 217
Fragments arranged by size:
600 568 217 94
NgoMIV G'CCGG_C
Cuts at: 0 474 621 1479
Size: 474 147 858
Fragments arranged by size:
858 474 147
PasI CC'CwG_GG
Cuts at: 0 509 1479
Size: 509 970
PflMI CCAn_nnn'nTGG
Cuts at: 0 834 1479
Size: 834 645
PfoI T'CCnGG_A
Cuts at: 0 542 1479
Size: 542 937
PshAI GACnn'nnGTC
Cuts at: 0 293 1479
Size: 293 1186
PspOMI G'GGCC_C
Cuts at: 0 703 1479
Size: 703 776
PstI C_TGCA'G
Cuts at: 0 311 797 1479
Size: 311 486 682
Fragments arranged by size:
682 486 311
PvuII CAG'CTG
Cuts at: 0 885 998 1086 1245 1479
Size: 885 113 88 159 234
Fragments arranged by size:
885 234 159 113 88
SacI G_AGCT'C
Cuts at: 0 758 1124 1479
Size: 758 366 355
Fragments arranged by size:
758 366 355
SacII CC_GC'GG
Cuts at: 0 1167 1319 1479
Size: 1167 152 160
Fragments arranged by size:
1167 160 152
SalI G'TCGA_C
Cuts at: 0 517 1479
Size: 517 962
SapI GCTCTTCn'nnn_
Cuts at: 0 1129 1479
Size: 1129 350
ScaI AGT'ACT
Cuts at: 0 940 1479
Size: 940 539
SfcI C'TryA_G
Cuts at: 0 307 432 555 793 1479
Size: 307 125 123 238 686
Fragments arranged by size:
686 307 238 125 123
SfoI GGC'GCC
Cuts at: 0 601 695 1263 1479
Size: 601 94 568 216
Fragments arranged by size:
601 568 216 94
SgrAI Cr'CCGG_yG
Cuts at: 0 323 1479
Size: 323 1156
SmlI C'TyrA_G
Cuts at: 0 718 1479
Size: 718 761
StuI AGG'CCT
Cuts at: 0 1438 1479
Size: 1438 41
StyI C'CwwG_G
Cuts at: 0 737 1479
Size: 737 742
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 880 1479
Size: 880 599
TatI w'GTAC_w
Cuts at: 0 938 1479
Size: 938 541
Tth111I GACn'n_nGTC
Cuts at: 0 375 1479
Size: 375 1104
ZraI GAC'GTC
Cuts at: 0 1236 1479
Size: 1236 243
Enzymes that do cut:
AatII AccI AcuI AfeI AflIII AhdI AleI ApaI
ApaLI AscI AvaI BanI BanII BbeI BbsI BcgI
BglI BglII Bme1580I BmgBI BplI BpmI Bpu10I BpuEI
BsaI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrBI BsrFI BssHII BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I FalI FseI FspI
HaeII Hin4I HincII KasI MluI MscI MslI MspA1I
NaeI NarI NgoMIV PasI PflMI PfoI PshAI PspOMI
PstI PvuII SacI SacII SalI SapI ScaI SfcI
SfoI SgrAI SmlI StuI StyI TaqII TatI Tth111I
ZraI
Enzymes that do not cut:
AarI Acc65I AclI AflII AgeI AlfI AloI AlwNI
ApoI AseI AsiSI AvrII BaeI BamHI BbvCI BciVI
BclI BfrBI BlpI BmrI BmtI BsaAI BsaBI BsaWI
BsaXI BsiWI BsmBI BspEI BsrDI BsrGI BssSI BstAPI
BstBI BstZ17I ClaI CspCI DraI DraIII EcoNI EcoRI
EcoRV FspAI HindIII HpaI KpnI MfeI MmeI NcoI
NdeI NheI NotI NruI NsiI NspI PacI PciI
PmeI PmlI PpiI PpuMI PsiI PspXI PsrI PvuI
RsrII SanDI SbfI SexAI SfiI SgfI SmaI SnaBI
SpeI SphI SrfI SspI SwaI TstI XbaI XcmI
XhoI XmaI XmnI