Restriction map for
pFN21AE4473
(Linear) (Six-base) MAPSORT of: KIEE4473.seq Check: 7480 from: 1 to: 1479
KIEE4473.orf
With 182 enzymes: SgfI *
March 22, 2012 18:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 733 874 1214 1479
Size: 733 141 340 265
Fragments arranged by size:
733 340 265 141
AatII G_ACGT'C
Cuts at: 0 377 412 1364 1479
Size: 377 35 952 115
Fragments arranged by size:
952 377 115 35
AccI GT'mk_AC
Cuts at: 0 277 1479
Size: 277 1202
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 1479
Size: 67 1412
AfeI AGC'GCT
Cuts at: 0 166 1324 1479
Size: 166 1158 155
Fragments arranged by size:
1158 166 155
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1141 1405 1479
Size: 1141 264 74
Fragments arranged by size:
1141 264 74
AlwNI CAG_nnn'CTG
Cuts at: 0 87 470 1479
Size: 87 383 1009
Fragments arranged by size:
1009 383 87
ApaLI G'TGCA_C
Cuts at: 0 1126 1479
Size: 1126 353
AvaI C'yCGr_G
Cuts at: 0 695 1479
Size: 695 784
BanI G'GyrC_C
Cuts at: 0 51 952 1202 1479
Size: 51 901 250 277
Fragments arranged by size:
901 277 250 51
BanII G_rGCy'C
Cuts at: 0 783 937 1175 1479
Size: 783 154 238 304
Fragments arranged by size:
783 304 238 154
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1309 1343 1479
Size: 1309 34 136
Fragments arranged by size:
1309 136 34
BfrBI ATG'CAT
Cuts at: 0 1433 1479
Size: 1433 46
BglI GCCn_nnn'nGGC
Cuts at: 0 1098 1479
Size: 1098 381
Bme1580I G_kGCm'C
Cuts at: 0 293 1130 1406 1479
Size: 293 837 276 73
Fragments arranged by size:
837 293 276 73
BmgBI CAC'GTC
Cuts at: 0 1125 1134 1479
Size: 1125 9 345
Fragments arranged by size:
1125 345 9
BmrI ACTGGGnnnn_n'
Cuts at: 0 225 278 1479
Size: 225 53 1201
Fragments arranged by size:
1201 225 53
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 588 595 1033 1479
Size: 588 7 438 446
Fragments arranged by size:
588 446 438 7
Bpu10I CC'TnA_GC
Cuts at: 0 1039 1479
Size: 1039 440
BsaAI yAC'GTr
Cuts at: 0 384 1479
Size: 384 1095
BsaHI Gr'CG_yC
Cuts at: 0 374 409 446 1055 1361 1479
Size: 374 35 37 609 306 118
Fragments arranged by size:
609 374 306 118 37 35
BsaWI w'CCGG_w
Cuts at: 0 13 1479
Size: 13 1466
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 676 706 924 954 1479
Size: 676 30 218 30 525
Fragments arranged by size:
676 525 218 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 169 421 1030 1369 1479
Size: 169 252 609 339 110
Fragments arranged by size:
609 339 252 169 110
BseYI C'CCAG_C
Cuts at: 0 30 1479
Size: 30 1449
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 156 1181 1479
Size: 139 17 1025 298
Fragments arranged by size:
1025 298 139 17
BsiEI CG_ry'CG
Cuts at: 0 792 1479
Size: 792 687
BsiHKAI G_wGCw'C
Cuts at: 0 347 683 783 937 1130 1226 1479
Size: 347 336 100 154 193 96 253
Fragments arranged by size:
347 336 253 193 154 100 96
Bsp1286I G_dGCh'C
Cuts at: 0 293 347 683 783 937 1130 1175 1226
Size: 293 54 336 100 154 193 45 51
Cuts at: 1226 1406 1479
Size: 180 73
Fragments arranged by size:
336 293 193 180 154 100 73 54
51 45
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 733 874 1214 1405 1479
Size: 733 141 340 191 74
Fragments arranged by size:
733 340 191 141 74
BsrBI CCG'CTC
Cuts at: 0 96 1043 1479
Size: 96 947 436
Fragments arranged by size:
947 436 96
BsrFI r'CCGG_y
Cuts at: 0 77 124 1479
Size: 77 47 1355
Fragments arranged by size:
1355 77 47
BstAPI GCAn_nnn'nTGC
Cuts at: 0 470 1479
Size: 470 1009
BstBI TT'CG_AA
Cuts at: 0 506 1479
Size: 506 973
BstXI CCAn_nnnn'nTGG
Cuts at: 0 321 971 1479
Size: 321 650 508
Fragments arranged by size:
650 508 321
BstYI r'GATC_y
Cuts at: 0 329 1479
Size: 329 1150
BtgI C'CryG_G
Cuts at: 0 365 1058 1229 1479
Size: 365 693 171 250
Fragments arranged by size:
693 365 250 171
BtsI GCAGTG_nn'
Cuts at: 0 471 744 893 929 1193 1479
Size: 471 273 149 36 264 286
Fragments arranged by size:
471 286 273 264 149 36
ClaI AT'CG_AT
Cuts at: 0 257 1479
Size: 257 1222
DraIII CAC_nnn'GTG
Cuts at: 0 1134 1479
Size: 1134 345
DrdI GACnn_nn'nnGTC
Cuts at: 0 184 1060 1479
Size: 184 876 419
Fragments arranged by size:
876 419 184
EaeI y'GGCC_r
Cuts at: 0 8 1104 1479
Size: 8 1096 375
Fragments arranged by size:
1096 375 8
EarI CTCTTCn'nnn_
Cuts at: 0 196 679 1412 1479
Size: 196 483 733 67
Fragments arranged by size:
733 483 196 67
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 184 1294 1479
Size: 184 1110 185
Fragments arranged by size:
1110 185 184
EcoICRI GAG'CTC
Cuts at: 0 781 935 1479
Size: 781 154 544
Fragments arranged by size:
781 544 154
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 588 595 1033 1479
Size: 67 521 7 438 446
Fragments arranged by size:
521 446 438 67 7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1267 1479
Size: 1267 212
EcoO109I rG'GnC_Cy
Cuts at: 0 186 1272 1314 1479
Size: 186 1086 42 165
Fragments arranged by size:
1086 186 165 42
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 363 1479
Size: 363 1116
FspI TGC'GCA
Cuts at: 0 1192 1479
Size: 1192 287
HaeII r_GCGC'y
Cuts at: 0 64 87 168 1326 1479
Size: 64 23 81 1158 153
Fragments arranged by size:
1158 153 81 64 23
HincII GTy'rAC
Cuts at: 0 264 278 450 513 1479
Size: 264 14 172 63 966
Fragments arranged by size:
966 264 172 63 14
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 644 881 1479
Size: 644 237 598
Fragments arranged by size:
644 598 237
MscI TGG'CCA
Cuts at: 0 1106 1479
Size: 1106 373
MslI CAynn'nnrTG
Cuts at: 0 319 1123 1479
Size: 319 804 356
Fragments arranged by size:
804 356 319
MspA1I CmG'CkG
Cuts at: 0 57 461 1379 1479
Size: 57 404 918 100
Fragments arranged by size:
918 404 100 57
NaeI GCC'GGC
Cuts at: 0 126 1479
Size: 126 1353
NcoI C'CATG_G
Cuts at: 0 1058 1479
Size: 1058 421
NgoMIV G'CCGG_C
Cuts at: 0 124 1479
Size: 124 1355
NsiI A_TGCA'T
Cuts at: 0 1435 1479
Size: 1435 44
NspI r_CATG'y
Cuts at: 0 708 987 1433 1479
Size: 708 279 446 46
Fragments arranged by size:
708 446 279 46
PflMI CCAn_nnn'nTGG
Cuts at: 0 1113 1263 1479
Size: 1113 150 216
Fragments arranged by size:
1113 216 150
PfoI T'CCnGG_A
Cuts at: 0 1262 1479
Size: 1262 217
PpuMI rG'GwC_Cy
Cuts at: 0 186 1314 1479
Size: 186 1128 165
Fragments arranged by size:
1128 186 165
PstI C_TGCA'G
Cuts at: 0 146 475 900 1211 1322 1479
Size: 146 329 425 311 111 157
Fragments arranged by size:
425 329 311 157 146 111
SacI G_AGCT'C
Cuts at: 0 783 937 1479
Size: 783 154 542
Fragments arranged by size:
783 542 154
SalI G'TCGA_C
Cuts at: 0 276 1479
Size: 276 1203
SbfI CC_TGCA'GG
Cuts at: 0 146 1479
Size: 146 1333
SfcI C'TryA_G
Cuts at: 0 142 471 896 1207 1318 1479
Size: 142 329 425 311 111 161
Fragments arranged by size:
425 329 311 161 142 111
SmaI CCC'GGG
Cuts at: 0 697 1479
Size: 697 782
SnaBI TAC'GTA
Cuts at: 0 384 1479
Size: 384 1095
SphI G_CATG'C
Cuts at: 0 708 1433 1479
Size: 708 725 46
Fragments arranged by size:
725 708 46
StuI AGG'CCT
Cuts at: 0 481 1453 1479
Size: 481 972 26
Fragments arranged by size:
972 481 26
StyI C'CwwG_G
Cuts at: 0 338 1058 1479
Size: 338 720 421
Fragments arranged by size:
720 421 338
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 545 806 940 1479
Size: 545 261 134 539
Fragments arranged by size:
545 539 261 134
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 924 956 1479
Size: 924 32 523
Fragments arranged by size:
924 523 32
Tth111I GACn'n_nGTC
Cuts at: 0 373 1479
Size: 373 1106
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 971 1479
Size: 971 508
XmaI C'CCGG_G
Cuts at: 0 695 1479
Size: 695 784
ZraI GAC'GTC
Cuts at: 0 375 410 1362 1479
Size: 375 35 952 117
Fragments arranged by size:
952 375 117 35
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AlfI AlwNI ApaLI
AvaI BanI BanII BcgI BfrBI BglI Bme1580I BmgBI
BmrI BpmI Bpu10I BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrFI
BstAPI BstBI BstXI BstYI BtgI BtsI ClaI DraIII
DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
FalI FspI HaeII HincII MmeI MscI MslI MspA1I
NaeI NcoI NgoMIV NsiI NspI PflMI PfoI PpuMI
PstI SacI SalI SbfI SfcI SmaI SnaBI SphI
StuI StyI TaqII TstI Tth111I XcmI XmaI ZraI
Enzymes that do not cut:
Acc65I AclI AflII AflIII AgeI AhdI AleI AloI
ApaI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BbsI BbvCI BciVI BclI BglII BlpI BmtI
BplI BpuEI BsaI BsaBI BsiWI BsmI BsmBI BspEI
BspHI BsrDI BsrGI BssHII BssSI BstEII BstZ17I Bsu36I
BtgZI CspCI DraI EagI EcoRI EcoRV FseI FspAI
Hin4I HindIII HpaI KasI KpnI MfeI MluI NarI
NdeI NheI NotI NruI PacI PasI PciI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
PvuII RsrII SacII SanDI SapI ScaI SexAI SfiI
SfoI SgfI SgrAI SmlI SpeI SrfI SspI SwaI
TatI XbaI XhoI XmnI