Restriction map for
pFN21AE4472
(Linear) (Six-base) MAPSORT of: KIEE4472.seq Check: 3064 from: 1 to: 1479
KIEE4472.orf
With 182 enzymes: SgfI *
March 22, 2012 18:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1457 1479
Size: 1457 22
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 361 595 607 1061 1479
Size: 361 234 12 454 418
Fragments arranged by size:
454 418 361 234 12
AflII C'TTAA_G
Cuts at: 0 1194 1479
Size: 1194 285
AflIII A'CryG_T
Cuts at: 0 314 1479
Size: 314 1165
AleI CACnn'nnGTG
Cuts at: 0 1294 1479
Size: 1294 185
ApoI r'AATT_y
Cuts at: 0 555 1090 1479
Size: 555 535 389
Fragments arranged by size:
555 535 389
AvaI C'yCGr_G
Cuts at: 0 1324 1400 1479
Size: 1324 76 79
Fragments arranged by size:
1324 79 76
BanI G'GyrC_C
Cuts at: 0 61 1479
Size: 61 1418
BbsI GAAGACnn'nnnn_
Cuts at: 0 318 849 1479
Size: 318 531 630
Fragments arranged by size:
630 531 318
BclI T'GATC_A
Cuts at: 0 378 1286 1479
Size: 378 908 193
Fragments arranged by size:
908 378 193
BglII A'GATC_T
Cuts at: 0 596 1040 1479
Size: 596 444 439
Fragments arranged by size:
596 444 439
BlpI GC'TnA_GC
Cuts at: 0 904 1479
Size: 904 575
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 420 1269 1479
Size: 420 849 210
Fragments arranged by size:
849 420 210
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 403 754 958 1450 1479
Size: 403 351 204 492 29
Fragments arranged by size:
492 403 351 204 29
Bpu10I CC'TnA_GC
Cuts at: 0 1442 1479
Size: 1442 37
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1012 1479
Size: 1012 467
BsaI GGTCTCn'nnnn_
Cuts at: 0 934 1479
Size: 934 545
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1052 1082 1479
Size: 1052 30 397
Fragments arranged by size:
1052 397 30
BseYI C'CCAG_C
Cuts at: 0 33 369 813 997 1479
Size: 33 336 444 184 482
Fragments arranged by size:
482 444 336 184 33
BsiHKAI G_wGCw'C
Cuts at: 0 1448 1479
Size: 1448 31
BsmBI CGTCTCn'nnnn_
Cuts at: 0 911 1479
Size: 911 568
Bsp1286I G_dGCh'C
Cuts at: 0 1448 1479
Size: 1448 31
BspHI T'CATG_A
Cuts at: 0 1346 1479
Size: 1346 133
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1457 1479
Size: 1457 22
BsrDI GCAATG_nn'
Cuts at: 0 45 1479
Size: 45 1434
BssSI C'ACGA_G
Cuts at: 0 70 124 1479
Size: 70 54 1355
Fragments arranged by size:
1355 70 54
BstAPI GCAn_nnn'nTGC
Cuts at: 0 86 1479
Size: 86 1393
BstXI CCAn_nnnn'nTGG
Cuts at: 0 813 1479
Size: 813 666
BstYI r'GATC_y
Cuts at: 0 596 1040 1479
Size: 596 444 439
Fragments arranged by size:
596 444 439
Bsu36I CC'TnA_GG
Cuts at: 0 1307 1479
Size: 1307 172
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 240 1479
Size: 240 1239
BtsI GCAGTG_nn'
Cuts at: 0 356 808 938 1020 1479
Size: 356 452 130 82 459
Fragments arranged by size:
459 452 356 130 82
EaeI y'GGCC_r
Cuts at: 0 207 632 869 891 1479
Size: 207 425 237 22 588
Fragments arranged by size:
588 425 237 207 22
EarI CTCTTCn'nnn_
Cuts at: 0 1136 1195 1375 1479
Size: 1136 59 180 104
Fragments arranged by size:
1136 180 104 59
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 361 403 595 607 754 958 1061 1450
Size: 361 42 192 12 147 204 103 389
Cuts at: 1450 1479
Size: 29
Fragments arranged by size:
389 361 204 192 147 103 42 29
12
EcoO109I rG'GnC_Cy
Cuts at: 0 335 780 1479
Size: 335 445 699
Fragments arranged by size:
699 445 335
EcoRV GAT'ATC
Cuts at: 0 177 1149 1479
Size: 177 972 330
Fragments arranged by size:
972 330 177
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1209 1479
Size: 1209 270
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 693 725 1052 1084 1300 1332 1383 1415
Size: 693 32 327 32 216 32 51 32
Cuts at: 1415 1479
Size: 64
Fragments arranged by size:
693 327 216 64 51 32 32 32
32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1259 1479
Size: 1259 220
MscI TGG'CCA
Cuts at: 0 209 871 1479
Size: 209 662 608
Fragments arranged by size:
662 608 209
MslI CAynn'nnrTG
Cuts at: 0 685 1294 1479
Size: 685 609 185
Fragments arranged by size:
685 609 185
MspA1I CmG'CkG
Cuts at: 0 435 546 1294 1479
Size: 435 111 748 185
Fragments arranged by size:
748 435 185 111
NspI r_CATG'y
Cuts at: 0 831 1479
Size: 831 648
PflMI CCAn_nnn'nTGG
Cuts at: 0 362 867 1479
Size: 362 505 612
Fragments arranged by size:
612 505 362
PfoI T'CCnGG_A
Cuts at: 0 884 1479
Size: 884 595
PpuMI rG'GwC_Cy
Cuts at: 0 335 780 1479
Size: 335 445 699
Fragments arranged by size:
699 445 335
PshAI GACnn'nnGTC
Cuts at: 0 1159 1479
Size: 1159 320
PspXI vC'TCGA_Gb
Cuts at: 0 1324 1479
Size: 1324 155
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 826 858 1479
Size: 826 32 621
Fragments arranged by size:
826 621 32
PstI C_TGCA'G
Cuts at: 0 1280 1424 1479
Size: 1280 144 55
Fragments arranged by size:
1280 144 55
PvuII CAG'CTG
Cuts at: 0 435 546 1479
Size: 435 111 933
Fragments arranged by size:
933 435 111
RsrII CG'GwC_CG
Cuts at: 0 461 1479
Size: 461 1018
SanDI GG'GwC_CC
Cuts at: 0 335 1479
Size: 335 1144
SapI GCTCTTCn'nnn_
Cuts at: 0 1195 1479
Size: 1195 284
SfcI C'TryA_G
Cuts at: 0 1124 1276 1420 1479
Size: 1124 152 144 59
Fragments arranged by size:
1124 152 144 59
SmlI C'TyrA_G
Cuts at: 0 1027 1194 1324 1400 1473 1479
Size: 1027 167 130 76 73 6
Fragments arranged by size:
1027 167 130 76 73 6
SphI G_CATG'C
Cuts at: 0 831 1479
Size: 831 648
StuI AGG'CCT
Cuts at: 0 1168 1479
Size: 1168 311
StyI C'CwwG_G
Cuts at: 0 134 204 513 872 1479
Size: 134 70 309 359 607
Fragments arranged by size:
607 359 309 134 70
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 478 521 1479
Size: 478 43 958
Fragments arranged by size:
958 478 43
TatI w'GTAC_w
Cuts at: 0 833 1479
Size: 833 646
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 217 267 1479
Size: 217 50 1212
Fragments arranged by size:
1212 217 50
XhoI C'TCGA_G
Cuts at: 0 1324 1400 1479
Size: 1324 76 79
Fragments arranged by size:
1324 79 76
Enzymes that do cut:
AarI AcuI AflII AflIII AleI ApoI AvaI BanI
BbsI BclI BglII BlpI BplI BpmI Bpu10I BpuEI
BsaI BsaXI BseYI BsiHKAI BsmBI Bsp1286I BspHI BspMI
BsrDI BssSI BstAPI BstXI BstYI Bsu36I BtgZI BtsI
EaeI EarI Eco57MI EcoO109I EcoRV FalI Hin4I MmeI
MscI MslI MspA1I NspI PflMI PfoI PpuMI PshAI
PspXI PsrI PstI PvuII RsrII SanDI SapI SfcI
SmlI SphI StuI StyI TaqII TatI XcmI XhoI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AgeI AhdI AlfI
AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BanII BbeI BbvCI BcgI BciVI BfrBI
BglI Bme1580I BmgBI BmrI BmtI BsaAI BsaBI BsaHI
BsaWI BseRI BsgI BsiEI BsiWI BsmI BspEI BsrBI
BsrFI BsrGI BssHII BstBI BstEII BstZ17I BtgI ClaI
CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoNI
EcoRI FseI FspI FspAI HaeII HincII HindIII HpaI
KasI KpnI MfeI MluI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PmeI PmlI PpiI PsiI PspOMI PvuI SacI SacII
SalI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI SspI SwaI TstI Tth111I
XbaI XmaI XmnI ZraI