Restriction map for
pFN21AE4469
(Linear) (Six-base) MAPSORT of: KIEE4469.seq Check: 8475 from: 1 to: 1470
KIEE4469.orf
With 182 enzymes: SgfI *
March 22, 2012 18:07 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 597 961 1470
Size: 597 364 509
Fragments arranged by size:
597 509 364
AgeI A'CCGG_T
Cuts at: 0 1090 1470
Size: 1090 380
AleI CACnn'nnGTG
Cuts at: 0 1093 1470
Size: 1093 377
ApaLI G'TGCA_C
Cuts at: 0 1054 1470
Size: 1054 416
ApoI r'AATT_y
Cuts at: 0 396 576 841 1198 1298 1470
Size: 396 180 265 357 100 172
Fragments arranged by size:
396 357 265 180 172 100
AvaI C'yCGr_G
Cuts at: 0 779 1470
Size: 779 691
BamHI G'GATC_C
Cuts at: 0 635 1470
Size: 635 835
BanII G_rGCy'C
Cuts at: 0 1009 1470
Size: 1009 461
BclI T'GATC_A
Cuts at: 0 803 981 1470
Size: 803 178 489
Fragments arranged by size:
803 489 178
BglII A'GATC_T
Cuts at: 0 1451 1470
Size: 1451 19
Bme1580I G_kGCm'C
Cuts at: 0 1058 1100 1153 1470
Size: 1058 42 53 317
Fragments arranged by size:
1058 317 53 42
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 78 1470
Size: 78 1392
BsaWI w'CCGG_w
Cuts at: 0 1090 1470
Size: 1090 380
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 917 947 1470
Size: 917 30 523
Fragments arranged by size:
917 523 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 92 289 429 475 1470
Size: 92 197 140 46 995
Fragments arranged by size:
995 197 140 92 46
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1325 1470
Size: 1325 145
BsiHKAI G_wGCw'C
Cuts at: 0 519 1058 1470
Size: 519 539 412
Fragments arranged by size:
539 519 412
Bsp1286I G_dGCh'C
Cuts at: 0 519 1009 1058 1100 1153 1470
Size: 519 490 49 42 53 317
Fragments arranged by size:
519 490 317 53 49 42
BsrDI GCAATG_nn'
Cuts at: 0 351 531 1470
Size: 351 180 939
Fragments arranged by size:
939 351 180
BsrFI r'CCGG_y
Cuts at: 0 1090 1470
Size: 1090 380
BssSI C'ACGA_G
Cuts at: 0 1057 1470
Size: 1057 413
BstYI r'GATC_y
Cuts at: 0 365 635 1061 1451 1470
Size: 365 270 426 390 19
Fragments arranged by size:
426 390 365 270 19
BtgI C'CryG_G
Cuts at: 0 631 1470
Size: 631 839
BtsI GCAGTG_nn'
Cuts at: 0 447 1112 1470
Size: 447 665 358
Fragments arranged by size:
665 447 358
ClaI AT'CG_AT
Cuts at: 0 791 1470
Size: 791 679
DraI TTT'AAA
Cuts at: 0 1391 1470
Size: 1391 79
DraIII CAC_nnn'GTG
Cuts at: 0 490 1470
Size: 490 980
EaeI y'GGCC_r
Cuts at: 0 1227 1470
Size: 1227 243
EarI CTCTTCn'nnn_
Cuts at: 0 110 282 406 968 1470
Size: 110 172 124 562 502
Fragments arranged by size:
562 502 172 124 110
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 78 597 961 1470
Size: 78 519 364 509
Fragments arranged by size:
519 509 364 78
EcoNI CCTnn'n_nnAGG
Cuts at: 0 457 1470
Size: 457 1013
EcoRI G'AATT_C
Cuts at: 0 841 1470
Size: 841 629
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 317 349 911 917 943 949 1416 1448
Size: 317 32 562 6 26 6 467 32
Cuts at: 1448 1470
Size: 22
Fragments arranged by size:
562 467 317 32 32 26 22 6
6
HincII GTy'rAC
Cuts at: 0 771 1470
Size: 771 699
HpaI GTT'AAC
Cuts at: 0 771 1470
Size: 771 699
MscI TGG'CCA
Cuts at: 0 1229 1470
Size: 1229 241
MslI CAynn'nnrTG
Cuts at: 0 223 236 589 1093 1470
Size: 223 13 353 504 377
Fragments arranged by size:
504 377 353 223 13
NcoI C'CATG_G
Cuts at: 0 631 1470
Size: 631 839
NspI r_CATG'y
Cuts at: 0 1017 1035 1470
Size: 1017 18 435
Fragments arranged by size:
1017 435 18
PfoI T'CCnGG_A
Cuts at: 0 968 1470
Size: 968 502
PsiI TTA'TAA
Cuts at: 0 566 1470
Size: 566 904
PstI C_TGCA'G
Cuts at: 0 1413 1470
Size: 1413 57
SfcI C'TryA_G
Cuts at: 0 69 127 869 1049 1409 1470
Size: 69 58 742 180 360 61
Fragments arranged by size:
742 360 180 69 61 58
SgrAI Cr'CCGG_yG
Cuts at: 0 1090 1470
Size: 1090 380
SphI G_CATG'C
Cuts at: 0 1017 1470
Size: 1017 453
SspI AAT'ATT
Cuts at: 0 849 1470
Size: 849 621
StyI C'CwwG_G
Cuts at: 0 476 631 1145 1470
Size: 476 155 514 325
Fragments arranged by size:
514 476 325 155
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 962 1470
Size: 962 508
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 90 122 1470
Size: 90 32 1348
Fragments arranged by size:
1348 90 32
Tth111I GACn'n_nGTC
Cuts at: 0 1081 1470
Size: 1081 389
XbaI T'CTAG_A
Cuts at: 0 1236 1470
Size: 1236 234
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 504 1470
Size: 504 966
XmnI GAAnn'nnTTC
Cuts at: 0 336 598 1470
Size: 336 262 872
Fragments arranged by size:
872 336 262
Enzymes that do cut:
AcuI AgeI AleI ApaLI ApoI AvaI BamHI BanII
BclI BglII Bme1580I BpmI BsaWI BsaXI BseRI BsgI
BsiHKAI Bsp1286I BsrDI BsrFI BssSI BstYI BtgI BtsI
ClaI DraI DraIII EaeI EarI Eco57MI EcoNI EcoRI
Hin4I HincII HpaI MscI MslI NcoI NspI PfoI
PsiI PstI SfcI SgrAI SphI SspI StyI TaqII
TstI Tth111I XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AhdI AlfI AloI AlwNI ApaI AscI AseI AsiSI
AvrII BaeI BanI BbeI BbsI BbvCI BcgI BciVI
BfrBI BglI BlpI BmgBI BmrI BmtI BplI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BseYI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrBI BsrGI BssHII
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI CspCI
DrdI EagI EciI EcoICRI EcoO109I EcoRV FalI FseI
FspI FspAI HaeII HindIII KasI KpnI MfeI MluI
MmeI MspA1I NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PspOMI PspXI PsrI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SmaI SmlI SnaBI SpeI
SrfI StuI SwaI TatI XhoI XmaI ZraI