Restriction map for
pFN21AE4466
(Linear) (Six-base) MAPSORT of: KIEE4466.seq Check: 8248 from: 1 to: 1464
KIEE4466.orf
With 182 enzymes: SgfI *
March 22, 2012 18:07 ..
AatII G_ACGT'C
Cuts at: 0 641 1464
Size: 641 823
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 228 394 991 1464
Size: 228 166 597 473
Fragments arranged by size:
597 473 228 166
AfeI AGC'GCT
Cuts at: 0 826 1464
Size: 826 638
AleI CACnn'nnGTG
Cuts at: 0 571 1464
Size: 571 893
AlwNI CAG_nnn'CTG
Cuts at: 0 541 1464
Size: 541 923
ApaI G_GGCC'C
Cuts at: 0 112 1464
Size: 112 1352
ApaLI G'TGCA_C
Cuts at: 0 1141 1464
Size: 1141 323
AvaI C'yCGr_G
Cuts at: 0 48 1464
Size: 48 1416
BamHI G'GATC_C
Cuts at: 0 378 842 1464
Size: 378 464 622
Fragments arranged by size:
622 464 378
BanI G'GyrC_C
Cuts at: 0 23 200 469 650 1464
Size: 23 177 269 181 814
Fragments arranged by size:
814 269 181 177 23
BanII G_rGCy'C
Cuts at: 0 112 580 794 1055 1464
Size: 112 468 214 261 409
Fragments arranged by size:
468 409 261 214 112
BbeI G_GCGC'C
Cuts at: 0 27 1464
Size: 27 1437
BbvCI CC'TCA_GC
Cuts at: 0 196 1464
Size: 196 1268
BglI GCCn_nnn'nGGC
Cuts at: 0 59 514 1453 1464
Size: 59 455 939 11
Fragments arranged by size:
939 455 59 11
BlpI GC'TnA_GC
Cuts at: 0 1049 1464
Size: 1049 415
Bme1580I G_kGCm'C
Cuts at: 0 112 474 1145 1464
Size: 112 362 671 319
Fragments arranged by size:
671 362 319 112
BmgBI CAC'GTC
Cuts at: 0 117 1464
Size: 117 1347
BmrI ACTGGGnnnn_n'
Cuts at: 0 198 1464
Size: 198 1266
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1280 1464
Size: 1280 184
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 334 554 856 1464
Size: 334 220 302 608
Fragments arranged by size:
608 334 302 220
Bpu10I CC'TnA_GC
Cuts at: 0 196 1464
Size: 196 1268
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 312 601 868 1464
Size: 312 289 267 596
Fragments arranged by size:
596 312 289 267
BsaI GGTCTCn'nnnn_
Cuts at: 0 1061 1464
Size: 1061 403
BsaAI yAC'GTr
Cuts at: 0 504 1431 1464
Size: 504 927 33
Fragments arranged by size:
927 504 33
BsaBI GATnn'nnATC
Cuts at: 0 286 1464
Size: 286 1178
BsaHI Gr'CG_yC
Cuts at: 0 24 98 167 638 1464
Size: 24 74 69 471 826
Fragments arranged by size:
826 471 74 69 24
BsaWI w'CCGG_w
Cuts at: 0 818 1464
Size: 818 646
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 184 214 1464
Size: 184 30 1250
Fragments arranged by size:
1250 184 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 100 1464
Size: 100 1364
BseYI C'CCAG_C
Cuts at: 0 534 866 1449 1464
Size: 534 332 583 15
Fragments arranged by size:
583 534 332 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 484 1464
Size: 484 980
BsiEI CG_ry'CG
Cuts at: 0 9 1464
Size: 9 1455
BsiHKAI G_wGCw'C
Cuts at: 0 794 1055 1145 1436 1464
Size: 794 261 90 291 28
Fragments arranged by size:
794 291 261 90 28
BsmI GAATG_Cn'
Cuts at: 0 439 1044 1464
Size: 439 605 420
Fragments arranged by size:
605 439 420
BsmBI CGTCTCn'nnnn_
Cuts at: 0 158 1464
Size: 158 1306
Bsp1286I G_dGCh'C
Cuts at: 0 112 474 580 794 1055 1145 1436 1464
Size: 112 362 106 214 261 90 291 28
Fragments arranged by size:
362 291 261 214 112 106 90 28
BspEI T'CCGG_A
Cuts at: 0 818 1464
Size: 818 646
BsrBI CCG'CTC
Cuts at: 0 806 1464
Size: 806 658
BsrDI GCAATG_nn'
Cuts at: 0 303 1464
Size: 303 1161
BsrFI r'CCGG_y
Cuts at: 0 169 1464
Size: 169 1295
BssSI C'ACGA_G
Cuts at: 0 1243 1464
Size: 1243 221
BstXI CCAn_nnnn'nTGG
Cuts at: 0 357 1322 1464
Size: 357 965 142
Fragments arranged by size:
965 357 142
BstYI r'GATC_y
Cuts at: 0 378 842 1310 1464
Size: 378 464 468 154
Fragments arranged by size:
468 464 378 154
BtgI C'CryG_G
Cuts at: 0 9 54 125 255 1043 1464
Size: 9 45 71 130 788 421
Fragments arranged by size:
788 421 130 71 45 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 190 1464
Size: 190 1274
BtsI GCAGTG_nn'
Cuts at: 0 705 898 1464
Size: 705 193 566
Fragments arranged by size:
705 566 193
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 237 272 1464
Size: 237 35 1192
Fragments arranged by size:
1192 237 35
DraIII CAC_nnn'GTG
Cuts at: 0 720 782 1464
Size: 720 62 682
Fragments arranged by size:
720 682 62
EaeI y'GGCC_r
Cuts at: 0 183 416 506 515 1205 1464
Size: 183 233 90 9 690 259
Fragments arranged by size:
690 259 233 183 90 9
EarI CTCTTCn'nnn_
Cuts at: 0 77 616 628 772 1027 1464
Size: 77 539 12 144 255 437
Fragments arranged by size:
539 437 255 144 77 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 94 1267 1464
Size: 94 1173 197
Fragments arranged by size:
1173 197 94
EcoICRI GAG'CTC
Cuts at: 0 792 1053 1464
Size: 792 261 411
Fragments arranged by size:
792 411 261
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 228 334 394 554 856 991 1464
Size: 228 106 60 160 302 135 473
Fragments arranged by size:
473 302 228 160 135 106 60
EcoO109I rG'GnC_Cy
Cuts at: 0 109 1446 1464
Size: 109 1337 18
Fragments arranged by size:
1337 109 18
HaeII r_GCGC'y
Cuts at: 0 27 459 592 828 966 1464
Size: 27 432 133 236 138 498
Fragments arranged by size:
498 432 236 138 133 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 189 221 628 660 1464
Size: 189 32 407 32 804
Fragments arranged by size:
804 407 189 32 32
HincII GTy'rAC
Cuts at: 0 642 1464
Size: 642 822
KasI G'GCGC_C
Cuts at: 0 23 1464
Size: 23 1441
MscI TGG'CCA
Cuts at: 0 508 517 1207 1464
Size: 508 9 690 257
Fragments arranged by size:
690 508 257 9
MslI CAynn'nnrTG
Cuts at: 0 340 355 571 1138 1297 1464
Size: 340 15 216 567 159 167
Fragments arranged by size:
567 340 216 167 159 15
MspA1I CmG'CkG
Cuts at: 0 11 103 127 182 538 1366 1453 1464
Size: 11 92 24 55 356 828 87 11
Fragments arranged by size:
828 356 92 87 55 24 11 11
NaeI GCC'GGC
Cuts at: 0 171 1464
Size: 171 1293
NarI GG'CG_CC
Cuts at: 0 24 1464
Size: 24 1440
NgoMIV G'CCGG_C
Cuts at: 0 169 1464
Size: 169 1295
PmlI CAC'GTG
Cuts at: 0 504 1464
Size: 504 960
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 841 873 1464
Size: 841 32 591
Fragments arranged by size:
841 591 32
PspOMI G'GGCC_C
Cuts at: 0 108 1464
Size: 108 1356
PspXI vC'TCGA_Gb
Cuts at: 0 48 1464
Size: 48 1416
PstI C_TGCA'G
Cuts at: 0 215 684 1464
Size: 215 469 780
Fragments arranged by size:
780 469 215
PvuII CAG'CTG
Cuts at: 0 1366 1464
Size: 1366 98
SacI G_AGCT'C
Cuts at: 0 794 1055 1464
Size: 794 261 409
Fragments arranged by size:
794 409 261
SacII CC_GC'GG
Cuts at: 0 12 128 1464
Size: 12 116 1336
Fragments arranged by size:
1336 116 12
SapI GCTCTTCn'nnn_
Cuts at: 0 628 1027 1464
Size: 628 399 437
Fragments arranged by size:
628 437 399
SbfI CC_TGCA'GG
Cuts at: 0 215 1464
Size: 215 1249
SexAI A'CCwGG_T
Cuts at: 0 1250 1464
Size: 1250 214
SfcI C'TryA_G
Cuts at: 0 211 384 680 1464
Size: 211 173 296 784
Fragments arranged by size:
784 296 211 173
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 514 1464
Size: 514 950
SfoI GGC'GCC
Cuts at: 0 25 1464
Size: 25 1439
SgrAI Cr'CCGG_yG
Cuts at: 0 169 1464
Size: 169 1295
SmlI C'TyrA_G
Cuts at: 0 48 291 616 847 1464
Size: 48 243 325 231 617
Fragments arranged by size:
617 325 243 231 48
StyI C'CwwG_G
Cuts at: 0 668 1464
Size: 668 796
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 457 880 1464
Size: 457 423 584
Fragments arranged by size:
584 457 423
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 184 216 1464
Size: 184 32 1248
Fragments arranged by size:
1248 184 32
Tth111I GACn'n_nGTC
Cuts at: 0 36 1464
Size: 36 1428
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1351 1464
Size: 1351 113
XhoI C'TCGA_G
Cuts at: 0 48 1464
Size: 48 1416
XmnI GAAnn'nnTTC
Cuts at: 0 928 1464
Size: 928 536
ZraI GAC'GTC
Cuts at: 0 639 1464
Size: 639 825
Enzymes that do cut:
AatII AcuI AfeI AleI AlwNI ApaI ApaLI AvaI
BamHI BanI BanII BbeI BbvCI BglI BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BsrBI BsrDI BsrFI
BssSI BstXI BstYI BtgI BtgZI BtsI CspCI DraIII
EaeI EarI EciI EcoICRI Eco57MI EcoO109I HaeII Hin4I
HincII KasI MscI MslI MspA1I NaeI NarI NgoMIV
PmlI PpiI PspOMI PspXI PstI PvuII SacI SacII
SapI SbfI SexAI SfcI SfiI SfoI SgrAI SmlI
StyI TaqII TstI Tth111I XcmI XhoI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AflII AflIII AgeI AhdI
AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI
BbsI BcgI BciVI BclI BfrBI BglII BmtI BsiWI
BspHI BspMI BsrGI BssHII BstAPI BstBI BstEII BstZ17I
Bsu36I ClaI DraI DrdI EagI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HindIII HpaI KpnI MfeI
MluI MmeI NcoI NdeI NheI NotI NruI NsiI
NspI PacI PasI PciI PflMI PfoI PmeI PpuMI
PshAI PsiI PsrI PvuI RsrII SalI SanDI ScaI
SgfI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI TatI XbaI XmaI