Restriction map for
pFN21AE4464
(Linear) (Six-base) MAPSORT of: KIEE4464.seq Check: 2796 from: 1 to: 1458
KIEE4464.orf
With 182 enzymes: SgfI *
March 22, 2012 18:06 ..
AccI GT'mk_AC
Cuts at: 0 536 640 1458
Size: 536 104 818
Fragments arranged by size:
818 536 104
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 759 937 1241 1407 1458
Size: 759 178 304 166 51
Fragments arranged by size:
759 304 178 166 51
AflIII A'CryG_T
Cuts at: 0 51 1458
Size: 51 1407
AhdI GACnn_n'nnGTC
Cuts at: 0 150 1458
Size: 150 1308
AlwNI CAG_nnn'CTG
Cuts at: 0 615 1458
Size: 615 843
ApoI r'AATT_y
Cuts at: 0 158 333 484 1458
Size: 158 175 151 974
Fragments arranged by size:
974 175 158 151
AvaI C'yCGr_G
Cuts at: 0 552 1370 1458
Size: 552 818 88
Fragments arranged by size:
818 552 88
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1243 1276 1458
Size: 1243 33 182
Fragments arranged by size:
1243 182 33
BamHI G'GATC_C
Cuts at: 0 940 1458
Size: 940 518
BanII G_rGCy'C
Cuts at: 0 626 758 1458
Size: 626 132 700
Fragments arranged by size:
700 626 132
BbsI GAAGACnn'nnnn_
Cuts at: 0 249 1458
Size: 249 1209
BbvCI CC'TCA_GC
Cuts at: 0 704 1458
Size: 704 754
BclI T'GATC_A
Cuts at: 0 96 1458
Size: 96 1362
BlpI GC'TnA_GC
Cuts at: 0 1024 1458
Size: 1024 434
Bme1580I G_kGCm'C
Cuts at: 0 448 1458
Size: 448 1010
BmrI ACTGGGnnnn_n'
Cuts at: 0 195 1229 1458
Size: 195 1034 229
Fragments arranged by size:
1034 229 195
BmtI G_CTAG'C
Cuts at: 0 1364 1458
Size: 1364 94
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 161 1127 1458
Size: 161 966 331
Fragments arranged by size:
966 331 161
Bpu10I CC'TnA_GC
Cuts at: 0 704 1458
Size: 704 754
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 204 1315 1458
Size: 204 1111 143
Fragments arranged by size:
1111 204 143
BsaAI yAC'GTr
Cuts at: 0 1259 1458
Size: 1259 199
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 346 376 1100 1130 1458
Size: 346 30 724 30 328
Fragments arranged by size:
724 346 328 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 997 1458
Size: 997 461
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 532 628 1458
Size: 532 96 830
Fragments arranged by size:
830 532 96
BsiHKAI G_wGCw'C
Cuts at: 0 1331 1458
Size: 1331 127
BsiWI C'GTAC_G
Cuts at: 0 1414 1458
Size: 1414 44
Bsp1286I G_dGCh'C
Cuts at: 0 448 626 758 1331 1458
Size: 448 178 132 573 127
Fragments arranged by size:
573 448 178 132 127
BspHI T'CATG_A
Cuts at: 0 99 1458
Size: 99 1359
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1205 1458
Size: 1205 253
BsrDI GCAATG_nn'
Cuts at: 0 177 1458
Size: 177 1281
BstBI TT'CG_AA
Cuts at: 0 843 1458
Size: 843 615
BstEII G'GTnAC_C
Cuts at: 0 894 1458
Size: 894 564
BstYI r'GATC_y
Cuts at: 0 940 1458
Size: 940 518
BstZ17I GTA'TAC
Cuts at: 0 641 1458
Size: 641 817
BtgI C'CryG_G
Cuts at: 0 581 1458
Size: 581 877
BtsI GCAGTG_nn'
Cuts at: 0 929 1364 1458
Size: 929 435 94
Fragments arranged by size:
929 435 94
ClaI AT'CG_AT
Cuts at: 0 530 1187 1458
Size: 530 657 271
Fragments arranged by size:
657 530 271
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 563 598 1458
Size: 563 35 860
Fragments arranged by size:
860 563 35
DraI TTT'AAA
Cuts at: 0 1251 1458
Size: 1251 207
EaeI y'GGCC_r
Cuts at: 0 593 1458
Size: 593 865
EarI CTCTTCn'nnn_
Cuts at: 0 1217 1458
Size: 1217 241
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 161 759 937 1127 1241 1407 1458
Size: 161 598 178 190 114 166 51
Fragments arranged by size:
598 190 178 166 161 114 51
EcoNI CCTnn'n_nnAGG
Cuts at: 0 874 1458
Size: 874 584
EcoO109I rG'GnC_Cy
Cuts at: 0 612 1458
Size: 612 846
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 789 927 1458
Size: 789 138 531
Fragments arranged by size:
789 531 138
HaeII r_GCGC'y
Cuts at: 0 602 1458
Size: 602 856
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 111 136 143 168 202 234 346 378
Size: 111 25 7 25 34 32 112 32
Cuts at: 378 1429 1458
Size: 1051 29
Fragments arranged by size:
1051 112 111 34 32 32 29 25
25 7
HincII GTy'rAC
Cuts at: 0 108 1338 1458
Size: 108 1230 120
Fragments arranged by size:
1230 120 108
HpaI GTT'AAC
Cuts at: 0 1338 1458
Size: 1338 120
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 1217 1458
Size: 43 1174 241
Fragments arranged by size:
1174 241 43
MscI TGG'CCA
Cuts at: 0 595 1458
Size: 595 863
MslI CAynn'nnrTG
Cuts at: 0 73 1458
Size: 73 1385
MspA1I CmG'CkG
Cuts at: 0 456 741 1458
Size: 456 285 717
Fragments arranged by size:
717 456 285
NheI G'CTAG_C
Cuts at: 0 1360 1458
Size: 1360 98
NruI TCG'CGA
Cuts at: 0 1356 1458
Size: 1356 102
NspI r_CATG'y
Cuts at: 0 55 1458
Size: 55 1403
PciI A'CATG_T
Cuts at: 0 51 1458
Size: 51 1407
PflMI CCAn_nnn'nTGG
Cuts at: 0 406 1458
Size: 406 1052
PfoI T'CCnGG_A
Cuts at: 0 1346 1458
Size: 1346 112
PpuMI rG'GwC_Cy
Cuts at: 0 612 1458
Size: 612 846
PspXI vC'TCGA_Gb
Cuts at: 0 552 1458
Size: 552 906
PstI C_TGCA'G
Cuts at: 0 455 1458
Size: 455 1003
PvuII CAG'CTG
Cuts at: 0 456 1458
Size: 456 1002
ScaI AGT'ACT
Cuts at: 0 780 802 1458
Size: 780 22 656
Fragments arranged by size:
780 656 22
SfcI C'TryA_G
Cuts at: 0 451 1458
Size: 451 1007
SmaI CCC'GGG
Cuts at: 0 1372 1458
Size: 1372 86
SmlI C'TyrA_G
Cuts at: 0 219 552 1330 1458
Size: 219 333 778 128
Fragments arranged by size:
778 333 219 128
SrfI GCCC'GGGC
Cuts at: 0 1372 1458
Size: 1372 86
SspI AAT'ATT
Cuts at: 0 315 1247 1458
Size: 315 932 211
Fragments arranged by size:
932 315 211
TatI w'GTAC_w
Cuts at: 0 778 800 1011 1458
Size: 778 22 211 447
Fragments arranged by size:
778 447 211 22
Tth111I GACn'n_nGTC
Cuts at: 0 676 1458
Size: 676 782
XhoI C'TCGA_G
Cuts at: 0 552 1458
Size: 552 906
XmaI C'CCGG_G
Cuts at: 0 1370 1458
Size: 1370 88
Enzymes that do cut:
AccI AcuI AflIII AhdI AlwNI ApoI AvaI BaeI
BamHI BanII BbsI BbvCI BclI BlpI Bme1580I BmrI
BmtI BpmI Bpu10I BpuEI BsaAI BsaXI BseRI BsgI
BsiHKAI BsiWI Bsp1286I BspHI BspMI BsrDI BstBI BstEII
BstYI BstZ17I BtgI BtsI ClaI CspCI DraI EaeI
EarI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII
HpaI MmeI MscI MslI MspA1I NheI NruI NspI
PciI PflMI PfoI PpuMI PspXI PstI PvuII ScaI
SfcI SmaI SmlI SrfI SspI TatI Tth111I XhoI
XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AleI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BanI BbeI BcgI BciVI BfrBI BglI BglII BmgBI
BplI BsaI BsaBI BsaHI BsaWI BseYI BsiEI BsmI
BsmBI BspEI BsrBI BsrFI BsrGI BssHII BssSI BstAPI
BstXI Bsu36I BtgZI DraIII DrdI EagI EciI EcoICRI
EcoRI EcoRV FseI FspI FspAI HindIII KasI KpnI
MfeI MluI NaeI NarI NcoI NdeI NgoMIV NotI
NsiI PacI PasI PmeI PmlI PpiI PshAI PsiI
PspOMI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI SexAI SfiI SfoI SgfI SgrAI SnaBI
SpeI SphI StuI StyI SwaI TaqII TstI XbaI
XcmI XmnI ZraI