Restriction map for
pFN21AE4462
(Linear) (Six-base) MAPSORT of: KIEE4462.seq Check: 291 from: 1 to: 1437
KIEE4462.orf
With 182 enzymes: SgfI *
March 22, 2012 18:06 ..
AccI GT'mk_AC
Cuts at: 0 653 1193 1437
Size: 653 540 244
Fragments arranged by size:
653 540 244
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 577 760 1162 1437
Size: 577 183 402 275
Fragments arranged by size:
577 402 275 183
AflIII A'CryG_T
Cuts at: 0 1076 1437
Size: 1076 361
AleI CACnn'nnGTG
Cuts at: 0 883 1437
Size: 883 554
AlwNI CAG_nnn'CTG
Cuts at: 0 600 948 1140 1437
Size: 600 348 192 297
Fragments arranged by size:
600 348 297 192
AvaI C'yCGr_G
Cuts at: 0 68 112 214 1437
Size: 68 44 102 1223
Fragments arranged by size:
1223 102 68 44
BamHI G'GATC_C
Cuts at: 0 210 576 1437
Size: 210 366 861
Fragments arranged by size:
861 366 210
BanI G'GyrC_C
Cuts at: 0 849 1357 1437
Size: 849 508 80
Fragments arranged by size:
849 508 80
BanII G_rGCy'C
Cuts at: 0 278 1437
Size: 278 1159
BbeI G_GCGC'C
Cuts at: 0 1361 1437
Size: 1361 76
BbsI GAAGACnn'nnnn_
Cuts at: 0 689 1437
Size: 689 748
BlpI GC'TnA_GC
Cuts at: 0 1150 1437
Size: 1150 287
Bme1580I G_kGCm'C
Cuts at: 0 854 1437
Size: 854 583
BmrI ACTGGGnnnn_n'
Cuts at: 0 1128 1437
Size: 1128 309
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 346 394 688 863 1437
Size: 346 48 294 175 574
Fragments arranged by size:
574 346 294 175 48
Bpu10I CC'TnA_GC
Cuts at: 0 957 1437
Size: 957 480
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 910 1437
Size: 910 527
BsaAI yAC'GTr
Cuts at: 0 1032 1437
Size: 1032 405
BsaHI Gr'CG_yC
Cuts at: 0 64 1358 1437
Size: 64 1294 79
Fragments arranged by size:
1294 79 64
BsaWI w'CCGG_w
Cuts at: 0 489 1437
Size: 489 948
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 268 612 1437
Size: 268 344 825
Fragments arranged by size:
825 344 268
BseYI C'CCAG_C
Cuts at: 0 381 385 580 853 1437
Size: 381 4 195 273 584
Fragments arranged by size:
584 381 273 195 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 972 1279 1437
Size: 972 307 158
Fragments arranged by size:
972 307 158
BsiWI C'GTAC_G
Cuts at: 0 1028 1437
Size: 1028 409
BsmI GAATG_Cn'
Cuts at: 0 766 1437
Size: 766 671
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1117 1437
Size: 1117 320
Bsp1286I G_dGCh'C
Cuts at: 0 278 854 1437
Size: 278 576 583
Fragments arranged by size:
583 576 278
BspEI T'CCGG_A
Cuts at: 0 489 1437
Size: 489 948
BsrDI GCAATG_nn'
Cuts at: 0 618 1437
Size: 618 819
BsrFI r'CCGG_y
Cuts at: 0 1265 1309 1360 1437
Size: 1265 44 51 77
Fragments arranged by size:
1265 77 51 44
BstXI CCAn_nnnn'nTGG
Cuts at: 0 313 1437
Size: 313 1124
BstYI r'GATC_y
Cuts at: 0 98 210 576 1169 1437
Size: 98 112 366 593 268
Fragments arranged by size:
593 366 268 112 98
BtgI C'CryG_G
Cuts at: 0 669 1437
Size: 669 768
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1320 1437
Size: 1320 117
BtsI GCAGTG_nn'
Cuts at: 0 711 946 1437
Size: 711 235 491
Fragments arranged by size:
711 491 235
DrdI GACnn_nn'nnGTC
Cuts at: 0 439 1437
Size: 439 998
EaeI y'GGCC_r
Cuts at: 0 119 557 1362 1437
Size: 119 438 805 75
Fragments arranged by size:
805 438 119 75
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 127 469 1437
Size: 127 342 968
Fragments arranged by size:
968 342 127
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 346 394 577 688 760 863 1162 1437
Size: 346 48 183 111 72 103 299 275
Fragments arranged by size:
346 299 275 183 111 103 72 48
EcoO109I rG'GnC_Cy
Cuts at: 0 250 462 1041 1437
Size: 250 212 579 396
Fragments arranged by size:
579 396 250 212
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 753 1437
Size: 753 684
FspI TGC'GCA
Cuts at: 0 973 1437
Size: 973 464
FspAI rTGC'GCAy
Cuts at: 0 973 1437
Size: 973 464
HaeII r_GCGC'y
Cuts at: 0 108 1266 1361 1437
Size: 108 1158 95 76
Fragments arranged by size:
1158 108 95 76
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 924 956 1437
Size: 924 32 481
Fragments arranged by size:
924 481 32
KasI G'GCGC_C
Cuts at: 0 1357 1437
Size: 1357 80
MscI TGG'CCA
Cuts at: 0 121 1437
Size: 121 1316
MslI CAynn'nnrTG
Cuts at: 0 883 1437
Size: 883 554
MspA1I CmG'CkG
Cuts at: 0 24 385 1003 1062 1295 1437
Size: 24 361 618 59 233 142
Fragments arranged by size:
618 361 233 142 59 24
NaeI GCC'GGC
Cuts at: 0 1267 1311 1362 1437
Size: 1267 44 51 75
Fragments arranged by size:
1267 75 51 44
NarI GG'CG_CC
Cuts at: 0 1358 1437
Size: 1358 79
NcoI C'CATG_G
Cuts at: 0 669 1437
Size: 669 768
NgoMIV G'CCGG_C
Cuts at: 0 1265 1309 1360 1437
Size: 1265 44 51 77
Fragments arranged by size:
1265 77 51 44
NspI r_CATG'y
Cuts at: 0 1080 1437
Size: 1080 357
PciI A'CATG_T
Cuts at: 0 1076 1437
Size: 1076 361
PflMI CCAn_nnn'nTGG
Cuts at: 0 897 1251 1437
Size: 897 354 186
Fragments arranged by size:
897 354 186
PfoI T'CCnGG_A
Cuts at: 0 890 1052 1437
Size: 890 162 385
Fragments arranged by size:
890 385 162
PpuMI rG'GwC_Cy
Cuts at: 0 250 1437
Size: 250 1187
PstI C_TGCA'G
Cuts at: 0 262 1437
Size: 262 1175
PvuII CAG'CTG
Cuts at: 0 385 1003 1062 1437
Size: 385 618 59 375
Fragments arranged by size:
618 385 375 59
SanDI GG'GwC_CC
Cuts at: 0 250 1437
Size: 250 1187
ScaI AGT'ACT
Cuts at: 0 145 1437
Size: 145 1292
SfcI C'TryA_G
Cuts at: 0 258 1437
Size: 258 1179
SfoI GGC'GCC
Cuts at: 0 1359 1437
Size: 1359 78
SmaI CCC'GGG
Cuts at: 0 114 1437
Size: 114 1323
SmlI C'TyrA_G
Cuts at: 0 925 1437
Size: 925 512
StyI C'CwwG_G
Cuts at: 0 669 1437
Size: 669 768
TatI w'GTAC_w
Cuts at: 0 143 329 494 1437
Size: 143 186 165 943
Fragments arranged by size:
943 186 165 143
XmaI C'CCGG_G
Cuts at: 0 112 1437
Size: 112 1325
XmnI GAAnn'nnTTC
Cuts at: 0 1324 1437
Size: 1324 113
Enzymes that do cut:
AccI AcuI AflIII AleI AlwNI AvaI BamHI BanI
BanII BbeI BbsI BlpI Bme1580I BmrI BpmI Bpu10I
BpuEI BsaAI BsaHI BsaWI BseRI BseYI BsgI BsiWI
BsmI BsmBI Bsp1286I BspEI BsrDI BsrFI BstXI BstYI
BtgI BtgZI BtsI DrdI EaeI EciI Eco57MI EcoO109I
FalI FspI FspAI HaeII Hin4I KasI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PfoI PpuMI PstI PvuII SanDI ScaI SfcI SfoI
SmaI SmlI StyI TatI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BmgBI BmtI BplI BsaI BsaBI BsaXI BsiEI
BsiHKAI BspHI BspMI BsrBI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI DraIII
EagI EarI EcoICRI EcoNI EcoRI EcoRV FseI HincII
HindIII HpaI KpnI MfeI MluI MmeI NdeI NheI
NotI NruI NsiI PacI PasI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SapI SbfI SexAI SfiI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII
TstI Tth111I XbaI XcmI XhoI ZraI