Restriction map for
pFN21AE4461
(Linear) (Six-base) MAPSORT of: KIEE4461.seq Check: 2163 from: 1 to: 1431
KIEE4461.orf
With 182 enzymes: SgfI *
March 22, 2012 18:06 ..
AccI GT'mk_AC
Cuts at: 0 1264 1431
Size: 1264 167
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 848 995 1228 1431
Size: 848 147 233 203
Fragments arranged by size:
848 233 203 147
AfeI AGC'GCT
Cuts at: 0 667 1431
Size: 667 764
AleI CACnn'nnGTG
Cuts at: 0 931 1431
Size: 931 500
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 461 1431
Size: 461 970
AlwNI CAG_nnn'CTG
Cuts at: 0 497 545 937 1128 1161 1431
Size: 497 48 392 191 33 270
Fragments arranged by size:
497 392 270 191 48 33
ApaI G_GGCC'C
Cuts at: 0 72 207 1179 1431
Size: 72 135 972 252
Fragments arranged by size:
972 252 135 72
ApaLI G'TGCA_C
Cuts at: 0 436 1431
Size: 436 995
AvaI C'yCGr_G
Cuts at: 0 173 410 1431
Size: 173 237 1021
Fragments arranged by size:
1021 237 173
AvrII C'CTAG_G
Cuts at: 0 198 1431
Size: 198 1233
BanI G'GyrC_C
Cuts at: 0 307 1002 1388 1431
Size: 307 695 386 43
Fragments arranged by size:
695 386 307 43
BanII G_rGCy'C
Cuts at: 0 72 198 207 533 779 972 1078 1179
Size: 72 126 9 326 246 193 106 101
Cuts at: 1179 1431
Size: 252
Fragments arranged by size:
326 252 246 193 126 106 101 72
9
BbeI G_GCGC'C
Cuts at: 0 1392 1431
Size: 1392 39
BbvCI CC'TCA_GC
Cuts at: 0 391 890 1431
Size: 391 499 541
Fragments arranged by size:
541 499 391
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 562 596 1431
Size: 562 34 835
Fragments arranged by size:
835 562 34
BglI GCCn_nnn'nGGC
Cuts at: 0 202 904 1082 1431
Size: 202 702 178 349
Fragments arranged by size:
702 349 202 178
BlpI GC'TnA_GC
Cuts at: 0 532 569 662 778 1431
Size: 532 37 93 116 653
Fragments arranged by size:
653 532 116 93 37
Bme1580I G_kGCm'C
Cuts at: 0 72 207 312 440 915 1179 1417 1431
Size: 72 135 105 128 475 264 238 14
Fragments arranged by size:
475 264 238 135 128 105 72 14
BmrI ACTGGGnnnn_n'
Cuts at: 0 548 604 1254 1431
Size: 548 56 650 177
Fragments arranged by size:
650 548 177 56
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 690 769 850 1150 1361 1431
Size: 690 79 81 300 211 70
Fragments arranged by size:
690 300 211 81 79 70
Bpu10I CC'TnA_GC
Cuts at: 0 61 391 890 1061 1431
Size: 61 330 499 171 370
Fragments arranged by size:
499 370 330 171 61
BsaI GGTCTCn'nnnn_
Cuts at: 0 260 1431
Size: 260 1171
BsaAI yAC'GTr
Cuts at: 0 300 1431
Size: 300 1131
BsaHI Gr'CG_yC
Cuts at: 0 475 1389 1431
Size: 475 914 42
Fragments arranged by size:
914 475 42
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1349 1379 1431
Size: 1349 30 52
Fragments arranged by size:
1349 52 30
BseYI C'CCAG_C
Cuts at: 0 21 273 385 449 606 1104 1431
Size: 21 252 112 64 157 498 327
Fragments arranged by size:
498 327 252 157 112 64 21
BsiHKAI G_wGCw'C
Cuts at: 0 440 533 872 1431
Size: 440 93 339 559
Fragments arranged by size:
559 440 339 93
BsmI GAATG_Cn'
Cuts at: 0 1253 1431
Size: 1253 178
BsmBI CGTCTCn'nnnn_
Cuts at: 0 482 1431
Size: 482 949
Bsp1286I G_dGCh'C
Cuts at: 0 72 198 207 312 440 533 779 872
Size: 72 126 9 105 128 93 246 93
Cuts at: 872 915 972 1078 1179 1417 1431
Size: 43 57 106 101 238 14
Fragments arranged by size:
246 238 128 126 106 105 101 93
93 72 57 43 14 9
BsrFI r'CCGG_y
Cuts at: 0 765 1431
Size: 765 666
BssSI C'ACGA_G
Cuts at: 0 908 1431
Size: 908 523
BstXI CCAn_nnnn'nTGG
Cuts at: 0 594 991 1431
Size: 594 397 440
Fragments arranged by size:
594 440 397
BstYI r'GATC_y
Cuts at: 0 1017 1431
Size: 1017 414
BstZ17I GTA'TAC
Cuts at: 0 1265 1431
Size: 1265 166
Bsu36I CC'TnA_GG
Cuts at: 0 83 334 467 710 1431
Size: 83 251 133 243 721
Fragments arranged by size:
721 251 243 133 83
BtgI C'CryG_G
Cuts at: 0 11 485 501 1431
Size: 11 474 16 930
Fragments arranged by size:
930 474 16 11
BtsI GCAGTG_nn'
Cuts at: 0 111 926 938 1224 1431
Size: 111 815 12 286 207
Fragments arranged by size:
815 286 207 111 12
DraIII CAC_nnn'GTG
Cuts at: 0 688 1431
Size: 688 743
EaeI y'GGCC_r
Cuts at: 0 44 1431
Size: 44 1387
EarI CTCTTCn'nnn_
Cuts at: 0 565 1431
Size: 565 866
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 41 337 633 1431
Size: 41 296 296 798
Fragments arranged by size:
798 296 296 41
EcoICRI GAG'CTC
Cuts at: 0 531 1431
Size: 531 900
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 690 769 848 850 995 1150 1228 1361
Size: 690 79 79 2 145 155 78 133
Cuts at: 1361 1431
Size: 70
Fragments arranged by size:
690 155 145 133 79 79 78 70
2
EcoO109I rG'GnC_Cy
Cuts at: 0 69 142 203 542 714 1431
Size: 69 73 61 339 172 717
Fragments arranged by size:
717 339 172 73 69 61
HaeII r_GCGC'y
Cuts at: 0 456 669 801 1392 1431
Size: 456 213 132 591 39
Fragments arranged by size:
591 456 213 132 39
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 460 492 693 725 1431
Size: 460 32 201 32 706
Fragments arranged by size:
706 460 201 32 32
KasI G'GCGC_C
Cuts at: 0 1388 1431
Size: 1388 43
MslI CAynn'nnrTG
Cuts at: 0 931 1431
Size: 931 500
MspA1I CmG'CkG
Cuts at: 0 38 190 277 1173 1189 1431
Size: 38 152 87 896 16 242
Fragments arranged by size:
896 242 152 87 38 16
NarI GG'CG_CC
Cuts at: 0 1389 1431
Size: 1389 42
NcoI C'CATG_G
Cuts at: 0 485 1431
Size: 485 946
NspI r_CATG'y
Cuts at: 0 319 1431
Size: 319 1112
PflMI CCAn_nnn'nTGG
Cuts at: 0 657 1230 1431
Size: 657 573 201
Fragments arranged by size:
657 573 201
PfoI T'CCnGG_A
Cuts at: 0 1020 1431
Size: 1020 411
PpuMI rG'GwC_Cy
Cuts at: 0 542 1431
Size: 542 889
PshAI GACnn'nnGTC
Cuts at: 0 13 824 1431
Size: 13 811 607
Fragments arranged by size:
811 607 13
PspOMI G'GGCC_C
Cuts at: 0 68 203 1175 1431
Size: 68 135 972 256
Fragments arranged by size:
972 256 135 68
PstI C_TGCA'G
Cuts at: 0 933 959 1431
Size: 933 26 472
Fragments arranged by size:
933 472 26
PvuII CAG'CTG
Cuts at: 0 277 1189 1431
Size: 277 912 242
Fragments arranged by size:
912 277 242
RsrII CG'GwC_CG
Cuts at: 0 8 1431
Size: 8 1423
SacI G_AGCT'C
Cuts at: 0 533 1431
Size: 533 898
SfcI C'TryA_G
Cuts at: 0 929 938 955 1431
Size: 929 9 17 476
Fragments arranged by size:
929 476 17 9
SfoI GGC'GCC
Cuts at: 0 1390 1431
Size: 1390 41
SmaI CCC'GGG
Cuts at: 0 412 1431
Size: 412 1019
SpeI A'CTAG_T
Cuts at: 0 733 1431
Size: 733 698
SphI G_CATG'C
Cuts at: 0 319 1431
Size: 319 1112
StuI AGG'CCT
Cuts at: 0 655 1431
Size: 655 776
StyI C'CwwG_G
Cuts at: 0 198 459 485 656 963 1431
Size: 198 261 26 171 307 468
Fragments arranged by size:
468 307 261 198 171 26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 287 1431
Size: 287 1144
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 455 487 986 1018 1431
Size: 455 32 499 32 413
Fragments arranged by size:
499 455 413 32 32
XbaI T'CTAG_A
Cuts at: 0 696 1431
Size: 696 735
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 457 769 1431
Size: 457 312 662
Fragments arranged by size:
662 457 312
XmaI C'CCGG_G
Cuts at: 0 410 1431
Size: 410 1021
XmnI GAAnn'nnTTC
Cuts at: 0 289 1431
Size: 289 1142
Enzymes that do cut:
AccI AcuI AfeI AleI AlfI AlwNI ApaI ApaLI
AvaI AvrII BanI BanII BbeI BbvCI BcgI BglI
BlpI Bme1580I BmrI BpmI Bpu10I BsaI BsaAI BsaHI
BsaXI BseYI BsiHKAI BsmI BsmBI Bsp1286I BsrFI BssSI
BstXI BstYI BstZ17I Bsu36I BtgI BtsI DraIII EaeI
EarI EciI EcoICRI Eco57MI EcoO109I HaeII Hin4I KasI
MslI MspA1I NarI NcoI NspI PflMI PfoI PpuMI
PshAI PspOMI PstI PvuII RsrII SacI SfcI SfoI
SmaI SpeI SphI StuI StyI TaqII TstI XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AflIII AgeI AhdI
AloI ApoI AscI AseI AsiSI BaeI BamHI BbsI
BciVI BclI BfrBI BglII BmgBI BmtI BplI BpuEI
BsaBI BsaWI BseRI BsgI BsiEI BsiWI BspEI BspHI
BspMI BsrBI BsrDI BsrGI BssHII BstAPI BstBI BstEII
BtgZI ClaI CspCI DraI DrdI EagI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI NaeI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PmeI
PmlI PpiI PsiI PspXI PsrI PvuI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI
SmlI SnaBI SrfI SspI SwaI TatI Tth111I XhoI
ZraI