Restriction map for
pFN21AE4459
(Linear) (Six-base) MAPSORT of: KIEE4459.seq Check: 1539 from: 1 to: 1428
KIEE4459.orf
With 182 enzymes: SgfI *
March 22, 2012 18:05 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1070 1081 1428
Size: 1070 11 347
Fragments arranged by size:
1070 347 11
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 40 1428
Size: 40 1388
AlwNI CAG_nnn'CTG
Cuts at: 0 1004 1428
Size: 1004 424
ApaLI G'TGCA_C
Cuts at: 0 207 1428
Size: 207 1221
ApoI r'AATT_y
Cuts at: 0 316 1422 1428
Size: 316 1106 6
Fragments arranged by size:
1106 316 6
AvaI C'yCGr_G
Cuts at: 0 88 169 845 1428
Size: 88 81 676 583
Fragments arranged by size:
676 583 88 81
BanI G'GyrC_C
Cuts at: 0 70 93 116 1428
Size: 70 23 23 1312
Fragments arranged by size:
1312 70 23 23
BanII G_rGCy'C
Cuts at: 0 89 263 1428
Size: 89 174 1165
Fragments arranged by size:
1165 174 89
BbeI G_GCGC'C
Cuts at: 0 74 97 120 1428
Size: 74 23 23 1308
Fragments arranged by size:
1308 74 23 23
BbsI GAAGACnn'nnnn_
Cuts at: 0 1069 1428
Size: 1069 359
BbvCI CC'TCA_GC
Cuts at: 0 1269 1428
Size: 1269 159
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 132 166 1428
Size: 132 34 1262
Fragments arranged by size:
1262 132 34
BfrBI ATG'CAT
Cuts at: 0 339 1428
Size: 339 1089
BglII A'GATC_T
Cuts at: 0 555 1428
Size: 555 873
BlpI GC'TnA_GC
Cuts at: 0 1027 1428
Size: 1027 401
Bme1580I G_kGCm'C
Cuts at: 0 45 211 1428
Size: 45 166 1217
Fragments arranged by size:
1217 166 45
BmrI ACTGGGnnnn_n'
Cuts at: 0 378 1080 1428
Size: 378 702 348
Fragments arranged by size:
702 378 348
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 385 1428
Size: 385 1043
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 989 1251 1259 1366 1428
Size: 989 262 8 107 62
Fragments arranged by size:
989 262 107 62 8
Bpu10I CC'TnA_GC
Cuts at: 0 994 1269 1428
Size: 994 275 159
Fragments arranged by size:
994 275 159
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 499 1126 1428
Size: 499 627 302
Fragments arranged by size:
627 499 302
BsaI GGTCTCn'nnnn_
Cuts at: 0 676 743 1136 1428
Size: 676 67 393 292
Fragments arranged by size:
676 393 292 67
BsaAI yAC'GTr
Cuts at: 0 597 1428
Size: 597 831
BsaHI Gr'CG_yC
Cuts at: 0 71 94 117 293 824 1428
Size: 71 23 23 176 531 604
Fragments arranged by size:
604 531 176 71 23 23
BsaWI w'CCGG_w
Cuts at: 0 563 1428
Size: 563 865
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 130 160 740 770 777 796 807 826
Size: 130 30 580 30 7 19 11 19
Cuts at: 826 1072 1102 1428
Size: 246 30 326
Fragments arranged by size:
580 326 246 130 30 30 30 19
19 11 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 761 801 1136 1387 1428
Size: 761 40 335 251 41
Fragments arranged by size:
761 335 251 41 40
BseYI C'CCAG_C
Cuts at: 0 1327 1428
Size: 1327 101
BsiHKAI G_wGCw'C
Cuts at: 0 211 263 798 1428
Size: 211 52 535 630
Fragments arranged by size:
630 535 211 52
BsmI GAATG_Cn'
Cuts at: 0 449 1173 1428
Size: 449 724 255
Fragments arranged by size:
724 449 255
BsmBI CGTCTCn'nnnn_
Cuts at: 0 503 772 1428
Size: 503 269 656
Fragments arranged by size:
656 503 269
Bsp1286I G_dGCh'C
Cuts at: 0 45 89 211 263 798 1428
Size: 45 44 122 52 535 630
Fragments arranged by size:
630 535 122 52 45 44
BspHI T'CATG_A
Cuts at: 0 798 1428
Size: 798 630
BsrDI GCAATG_nn'
Cuts at: 0 546 1428
Size: 546 882
BsrFI r'CCGG_y
Cuts at: 0 119 1428
Size: 119 1309
BssHII G'CGCG_C
Cuts at: 0 20 1428
Size: 20 1408
BssSI C'ACGA_G
Cuts at: 0 187 1428
Size: 187 1241
BstAPI GCAn_nnn'nTGC
Cuts at: 0 49 1428
Size: 49 1379
BstEII G'GTnAC_C
Cuts at: 0 1153 1428
Size: 1153 275
BstXI CCAn_nnnn'nTGG
Cuts at: 0 304 1182 1428
Size: 304 878 246
Fragments arranged by size:
878 304 246
BstYI r'GATC_y
Cuts at: 0 555 1428
Size: 555 873
BtgI C'CryG_G
Cuts at: 0 98 741 1019 1181 1428
Size: 98 643 278 162 247
Fragments arranged by size:
643 278 247 162 98
BtsI GCAGTG_nn'
Cuts at: 0 211 1428
Size: 211 1217
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1283 1318 1428
Size: 1283 35 110
Fragments arranged by size:
1283 110 35
DraI TTT'AAA
Cuts at: 0 321 1421 1428
Size: 321 1100 7
Fragments arranged by size:
1100 321 7
EaeI y'GGCC_r
Cuts at: 0 2 758 869 889 1428
Size: 2 756 111 20 539
Fragments arranged by size:
756 539 111 20 2
EarI CTCTTCn'nnn_
Cuts at: 0 861 1046 1364 1428
Size: 861 185 318 64
Fragments arranged by size:
861 318 185 64
EcoICRI GAG'CTC
Cuts at: 0 261 1428
Size: 261 1167
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 989 1070 1081 1251 1259 1366 1428
Size: 989 81 11 170 8 107 62
Fragments arranged by size:
989 170 107 81 62 11 8
HaeII r_GCGC'y
Cuts at: 0 74 97 120 187 1428
Size: 74 23 23 67 1241
Fragments arranged by size:
1241 74 67 23 23
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 130 162 1192 1224 1428
Size: 130 32 1030 32 204
Fragments arranged by size:
1030 204 130 32 32
HincII GTy'rAC
Cuts at: 0 429 1428
Size: 429 999
HindIII A'AGCT_T
Cuts at: 0 670 837 1428
Size: 670 167 591
Fragments arranged by size:
670 591 167
KasI G'GCGC_C
Cuts at: 0 70 93 116 1428
Size: 70 23 23 1312
Fragments arranged by size:
1312 70 23 23
MfeI C'AATT_G
Cuts at: 0 422 1160 1428
Size: 422 738 268
Fragments arranged by size:
738 422 268
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 305 336 1428
Size: 305 31 1092
Fragments arranged by size:
1092 305 31
MscI TGG'CCA
Cuts at: 0 760 1428
Size: 760 668
MslI CAynn'nnrTG
Cuts at: 0 344 1180 1428
Size: 344 836 248
Fragments arranged by size:
836 344 248
MspA1I CmG'CkG
Cuts at: 0 100 1428
Size: 100 1328
NaeI GCC'GGC
Cuts at: 0 121 1428
Size: 121 1307
NarI GG'CG_CC
Cuts at: 0 71 94 117 1428
Size: 71 23 23 1311
Fragments arranged by size:
1311 71 23 23
NcoI C'CATG_G
Cuts at: 0 741 1428
Size: 741 687
NdeI CA'TA_TG
Cuts at: 0 771 1428
Size: 771 657
NgoMIV G'CCGG_C
Cuts at: 0 119 1428
Size: 119 1309
NsiI A_TGCA'T
Cuts at: 0 341 1428
Size: 341 1087
PasI CC'CwG_GG
Cuts at: 0 546 1428
Size: 546 882
PflMI CCAn_nnn'nTGG
Cuts at: 0 840 1428
Size: 840 588
PshAI GACnn'nnGTC
Cuts at: 0 424 1428
Size: 424 1004
PstI C_TGCA'G
Cuts at: 0 131 206 1428
Size: 131 75 1222
Fragments arranged by size:
1222 131 75
SacI G_AGCT'C
Cuts at: 0 263 1428
Size: 263 1165
SacII CC_GC'GG
Cuts at: 0 101 1428
Size: 101 1327
SfcI C'TryA_G
Cuts at: 0 127 202 1428
Size: 127 75 1226
Fragments arranged by size:
1226 127 75
SfoI GGC'GCC
Cuts at: 0 72 95 118 1428
Size: 72 23 23 1310
Fragments arranged by size:
1310 72 23 23
SgrAI Cr'CCGG_yG
Cuts at: 0 119 1428
Size: 119 1309
SmaI CCC'GGG
Cuts at: 0 90 847 1428
Size: 90 757 581
Fragments arranged by size:
757 581 90
SmlI C'TyrA_G
Cuts at: 0 514 1105 1428
Size: 514 591 323
Fragments arranged by size:
591 514 323
SrfI GCCC'GGGC
Cuts at: 0 847 1428
Size: 847 581
StyI C'CwwG_G
Cuts at: 0 686 741 1428
Size: 686 55 687
Fragments arranged by size:
687 686 55
SwaI ATTT'AAAT
Cuts at: 0 321 1428
Size: 321 1107
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 740 772 777 809 1428
Size: 740 32 5 32 619
Fragments arranged by size:
740 619 32 32 5
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1179 1428
Size: 1179 249
XmaI C'CCGG_G
Cuts at: 0 88 845 1428
Size: 88 757 583
Fragments arranged by size:
757 583 88
Enzymes that do cut:
AcuI AlfI AlwNI ApaLI ApoI AvaI BanI BanII
BbeI BbsI BbvCI BcgI BfrBI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BsrDI BsrFI BssHII BssSI BstAPI BstEII BstXI
BstYI BtgI BtsI CspCI DraI EaeI EarI EcoICRI
Eco57MI HaeII Hin4I HincII HindIII KasI MfeI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NsiI PasI PflMI PshAI PstI SacI SacII SfcI
SfoI SgrAI SmaI SmlI SrfI StyI SwaI TstI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AloI ApaI AscI AseI AsiSI
AvrII BaeI BamHI BciVI BclI BglI BmgBI BmtI
BsaBI BsgI BsiEI BsiWI BspEI BspMI BsrBI BsrGI
BstBI BstZ17I Bsu36I BtgZI ClaI DraIII DrdI EagI
EciI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI
FspAI HpaI KpnI MluI NheI NotI NruI NspI
PacI PciI PfoI PmeI PmlI PpiI PpuMI PsiI
PspOMI PspXI PsrI PvuI PvuII RsrII SalI SanDI
SapI SbfI ScaI SexAI SfiI SgfI SnaBI SpeI
SphI SspI StuI TaqII TatI Tth111I XbaI XhoI
XmnI ZraI