Restriction map for
pFN21AE4454
(Linear) (Six-base) MAPSORT of: KIEE4454.seq Check: 7359 from: 1 to: 1422
KIEE4454.orf
With 182 enzymes: SgfI *
March 22, 2012 18:04 ..
AccI GT'mk_AC
Cuts at: 0 828 849 1422
Size: 828 21 573
Fragments arranged by size:
828 573 21
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1177 1422
Size: 1177 245
AflIII A'CryG_T
Cuts at: 0 383 492 1422
Size: 383 109 930
Fragments arranged by size:
930 383 109
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 212 1422
Size: 212 1210
AlwNI CAG_nnn'CTG
Cuts at: 0 1146 1422
Size: 1146 276
ApaI G_GGCC'C
Cuts at: 0 78 1422
Size: 78 1344
ApaLI G'TGCA_C
Cuts at: 0 484 1422
Size: 484 938
AvaI C'yCGr_G
Cuts at: 0 1250 1422
Size: 1250 172
BanII G_rGCy'C
Cuts at: 0 78 116 593 1422
Size: 78 38 477 829
Fragments arranged by size:
829 477 78 38
BbvCI CC'TCA_GC
Cuts at: 0 83 97 1422
Size: 83 14 1325
Fragments arranged by size:
1325 83 14
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1045 1079 1327 1361 1422
Size: 1045 34 248 34 61
Fragments arranged by size:
1045 248 61 34 34
BlpI GC'TnA_GC
Cuts at: 0 931 1422
Size: 931 491
Bme1580I G_kGCm'C
Cuts at: 0 78 180 488 883 1422
Size: 78 102 308 395 539
Fragments arranged by size:
539 395 308 102 78
BmgBI CAC'GTC
Cuts at: 0 847 1422
Size: 847 575
BmrI ACTGGGnnnn_n'
Cuts at: 0 21 167 1422
Size: 21 146 1255
Fragments arranged by size:
1255 146 21
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1194 1422
Size: 1194 228
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 277 877 970 1422
Size: 205 72 600 93 452
Fragments arranged by size:
600 452 205 93 72
Bpu10I CC'TnA_GC
Cuts at: 0 83 97 1208 1422
Size: 83 14 1111 214
Fragments arranged by size:
1111 214 83 14
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 614 1422
Size: 614 808
BsaI GGTCTCn'nnnn_
Cuts at: 0 743 1422
Size: 743 679
BsaAI yAC'GTr
Cuts at: 0 1332 1422
Size: 1332 90
BsaBI GATnn'nnATC
Cuts at: 0 430 1422
Size: 430 992
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1382 1412 1422
Size: 1382 30 10
Fragments arranged by size:
1382 30 10
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 61 1183 1422
Size: 61 1122 239
Fragments arranged by size:
1122 239 61
BseYI C'CCAG_C
Cuts at: 0 411 477 550 896 904 1275 1422
Size: 411 66 73 346 8 371 147
Fragments arranged by size:
411 371 346 147 73 66 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 940 1422
Size: 223 717 482
Fragments arranged by size:
717 482 223
BsiHKAI G_wGCw'C
Cuts at: 0 488 593 1422
Size: 488 105 829
Fragments arranged by size:
829 488 105
BsmBI CGTCTCn'nnnn_
Cuts at: 0 310 1422
Size: 310 1112
Bsp1286I G_dGCh'C
Cuts at: 0 78 116 180 488 593 883 1422
Size: 78 38 64 308 105 290 539
Fragments arranged by size:
539 308 290 105 78 64 38
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 227 1422
Size: 227 1195
BsrBI CCG'CTC
Cuts at: 0 301 1422
Size: 301 1121
BsrDI GCAATG_nn'
Cuts at: 0 341 887 1422
Size: 341 546 535
Fragments arranged by size:
546 535 341
BsrFI r'CCGG_y
Cuts at: 0 1076 1422
Size: 1076 346
BssSI C'ACGA_G
Cuts at: 0 1315 1422
Size: 1315 107
BstBI TT'CG_AA
Cuts at: 0 1347 1422
Size: 1347 75
BstXI CCAn_nnnn'nTGG
Cuts at: 0 612 900 904 1422
Size: 612 288 4 518
Fragments arranged by size:
612 518 288 4
BstYI r'GATC_y
Cuts at: 0 533 1422
Size: 533 889
Bsu36I CC'TnA_GG
Cuts at: 0 974 1422
Size: 974 448
BtgI C'CryG_G
Cuts at: 0 65 1422
Size: 65 1357
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1035 1422
Size: 1035 387
EaeI y'GGCC_r
Cuts at: 0 2 683 771 966 1422
Size: 2 681 88 195 456
Fragments arranged by size:
681 456 195 88 2
EarI CTCTTCn'nnn_
Cuts at: 0 160 1422
Size: 160 1262
EcoICRI GAG'CTC
Cuts at: 0 591 1422
Size: 591 831
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 277 877 970 1177 1422
Size: 205 72 600 93 207 245
Fragments arranged by size:
600 245 207 205 93 72
EcoNI CCTnn'n_nnAGG
Cuts at: 0 25 82 334 1422
Size: 25 57 252 1088
Fragments arranged by size:
1088 252 57 25
EcoO109I rG'GnC_Cy
Cuts at: 0 75 101 459 1059 1143 1175 1422
Size: 75 26 358 600 84 32 247
Fragments arranged by size:
600 358 247 84 75 32 26
EcoRV GAT'ATC
Cuts at: 0 723 1422
Size: 723 699
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 482 514 1382 1414 1422
Size: 482 32 868 32 8
Fragments arranged by size:
868 482 32 32 8
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 729 1422
Size: 729 693
MscI TGG'CCA
Cuts at: 0 4 685 773 968 1422
Size: 4 681 88 195 454
Fragments arranged by size:
681 454 195 88 4
MslI CAynn'nnrTG
Cuts at: 0 491 697 1189 1370 1422
Size: 491 206 492 181 52
Fragments arranged by size:
492 491 206 181 52
MspA1I CmG'CkG
Cuts at: 0 481 900 1155 1387 1422
Size: 481 419 255 232 35
Fragments arranged by size:
481 419 255 232 35
NspI r_CATG'y
Cuts at: 0 387 496 813 871 948 1369 1422
Size: 387 109 317 58 77 421 53
Fragments arranged by size:
421 387 317 109 77 58 53
PasI CC'CwG_GG
Cuts at: 0 567 1422
Size: 567 855
PciI A'CATG_T
Cuts at: 0 383 492 1422
Size: 383 109 930
Fragments arranged by size:
930 383 109
PflMI CCAn_nnn'nTGG
Cuts at: 0 11 584 1422
Size: 11 573 838
Fragments arranged by size:
838 573 11
PfoI T'CCnGG_A
Cuts at: 0 254 469 797 1400 1422
Size: 254 215 328 603 22
Fragments arranged by size:
603 328 254 215 22
PmlI CAC'GTG
Cuts at: 0 1332 1422
Size: 1332 90
PpuMI rG'GwC_Cy
Cuts at: 0 101 1422
Size: 101 1321
PspOMI G'GGCC_C
Cuts at: 0 74 1422
Size: 74 1348
PvuII CAG'CTG
Cuts at: 0 481 900 1155 1422
Size: 481 419 255 267
Fragments arranged by size:
481 419 267 255
SacI G_AGCT'C
Cuts at: 0 593 1422
Size: 593 829
SapI GCTCTTCn'nnn_
Cuts at: 0 160 1422
Size: 160 1262
SfcI C'TryA_G
Cuts at: 0 829 1422
Size: 829 593
SmlI C'TyrA_G
Cuts at: 0 629 1422
Size: 629 793
SphI G_CATG'C
Cuts at: 0 948 1369 1422
Size: 948 421 53
Fragments arranged by size:
948 421 53
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 618 767 910 1018 1422
Size: 618 149 143 108 404
Fragments arranged by size:
618 404 149 143 108
TatI w'GTAC_w
Cuts at: 0 821 1422
Size: 821 601
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 180 558 1102 1422
Size: 180 378 544 320
Fragments arranged by size:
544 378 320 180
Enzymes that do cut:
AccI AcuI AflIII AlfI AlwNI ApaI ApaLI AvaI
BanII BbvCI BcgI BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaXI BseRI
BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BssSI BstBI BstXI BstYI Bsu36I BtgI BtgZI
EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRV Hin4I
MmeI MscI MslI MspA1I NspI PasI PciI PflMI
PfoI PmlI PpuMI PspOMI PvuII SacI SapI SfcI
SmlI SphI TaqII TatI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AloI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BanI BbeI BbsI BciVI BclI BfrBI BglI
BglII BmtI BsaHI BsaWI BsiEI BsiWI BsmI BspEI
BspHI BsrGI BssHII BstAPI BstEII BstZ17I BtsI ClaI
CspCI DraI DraIII DrdI EagI EciI EcoRI FalI
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
KpnI MfeI MluI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PmeI PpiI PshAI
PsiI PspXI PsrI PstI PvuI RsrII SacII SalI
SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI SspI StuI StyI SwaI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI