Restriction map for
pFN21AE4453
(Linear) (Six-base) MAPSORT of: KIEE4453.seq Check: 233 from: 1 to: 1419
KIEE4453.orf
With 182 enzymes: SgfI *
March 22, 2012 18:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 16 1419
Size: 16 1403
AatII G_ACGT'C
Cuts at: 0 659 1419
Size: 659 760
Acc65I G'GTAC_C
Cuts at: 0 455 1419
Size: 455 964
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 198 588 751 1383 1419
Size: 198 390 163 632 36
Fragments arranged by size:
632 390 198 163 36
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1256 1419
Size: 1256 163
AlwNI CAG_nnn'CTG
Cuts at: 0 1026 1131 1419
Size: 1026 105 288
Fragments arranged by size:
1026 288 105
BanI G'GyrC_C
Cuts at: 0 455 1419
Size: 455 964
BanII G_rGCy'C
Cuts at: 0 49 1007 1390 1417 1419
Size: 49 958 383 27 2
Fragments arranged by size:
958 383 49 27 2
BbsI GAAGACnn'nnnn_
Cuts at: 0 1225 1320 1419
Size: 1225 95 99
Fragments arranged by size:
1225 99 95
BglI GCCn_nnn'nGGC
Cuts at: 0 589 1419
Size: 589 830
BglII A'GATC_T
Cuts at: 0 1202 1419
Size: 1202 217
BlpI GC'TnA_GC
Cuts at: 0 1051 1419
Size: 1051 368
Bme1580I G_kGCm'C
Cuts at: 0 126 1419
Size: 126 1293
BmrI ACTGGGnnnn_n'
Cuts at: 0 467 1419
Size: 467 952
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1404 1419
Size: 1404 15
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 15 66 433 730 1003 1048 1090 1102
Size: 15 51 367 297 273 45 42 12
Cuts at: 1102 1249 1282 1419
Size: 147 33 137
Fragments arranged by size:
367 297 273 147 137 51 45 42
33 15 12
Bpu10I CC'TnA_GC
Cuts at: 0 844 925 1419
Size: 844 81 494
Fragments arranged by size:
844 494 81
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1360 1419
Size: 1360 59
BsaI GGTCTCn'nnnn_
Cuts at: 0 907 1081 1419
Size: 907 174 338
Fragments arranged by size:
907 338 174
BsaBI GATnn'nnATC
Cuts at: 0 718 1419
Size: 718 701
BsaHI Gr'CG_yC
Cuts at: 0 656 1419
Size: 656 763
BsaWI w'CCGG_w
Cuts at: 0 1007 1419
Size: 1007 412
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1270 1300 1419
Size: 1270 30 119
Fragments arranged by size:
1270 119 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 18 84 147 159 430 751 781 784
Size: 18 66 63 12 271 321 30 3
Cuts at: 784 952 1099 1162 1326 1329 1419
Size: 168 147 63 164 3 90
Fragments arranged by size:
321 271 168 164 147 90 66 63
63 30 18 12 3 3
BseYI C'CCAG_C
Cuts at: 0 127 189 1046 1160 1289 1419
Size: 127 62 857 114 129 130
Fragments arranged by size:
857 130 129 127 114 62
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 812 1419
Size: 812 607
BsiHKAI G_wGCw'C
Cuts at: 0 411 1007 1019 1390 1417 1419
Size: 411 596 12 371 27 2
Fragments arranged by size:
596 411 371 27 12 2
BsmI GAATG_Cn'
Cuts at: 0 558 1419
Size: 558 861
BsmBI CGTCTCn'nnnn_
Cuts at: 0 899 1419
Size: 899 520
Bsp1286I G_dGCh'C
Cuts at: 0 49 126 411 1007 1019 1390 1417 1419
Size: 49 77 285 596 12 371 27 2
Fragments arranged by size:
596 371 285 77 49 27 12 2
BspEI T'CCGG_A
Cuts at: 0 1007 1419
Size: 1007 412
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 16 1419
Size: 16 1403
BsrDI GCAATG_nn'
Cuts at: 0 533 1419
Size: 533 886
BsrFI r'CCGG_y
Cuts at: 0 796 1419
Size: 796 623
BssSI C'ACGA_G
Cuts at: 0 763 1419
Size: 763 656
BstEII G'GTnAC_C
Cuts at: 0 354 1419
Size: 354 1065
BstYI r'GATC_y
Cuts at: 0 443 1121 1202 1419
Size: 443 678 81 217
Fragments arranged by size:
678 443 217 81
Bsu36I CC'TnA_GG
Cuts at: 0 520 751 1419
Size: 520 231 668
Fragments arranged by size:
668 520 231
BtsI GCAGTG_nn'
Cuts at: 0 351 996 1146 1419
Size: 351 645 150 273
Fragments arranged by size:
645 351 273 150
EaeI y'GGCC_r
Cuts at: 0 104 539 695 1302 1419
Size: 104 435 156 607 117
Fragments arranged by size:
607 435 156 117 104
EarI CTCTTCn'nnn_
Cuts at: 0 1353 1419
Size: 1353 66
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 815 1419
Size: 815 604
EcoICRI GAG'CTC
Cuts at: 0 1005 1388 1415 1419
Size: 1005 383 27 4
Fragments arranged by size:
1005 383 27 4
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 15 66 198 433 588 730 751 1003
Size: 15 51 132 235 155 142 21 252
Cuts at: 1003 1048 1090 1102 1249 1282 1383 1419
Size: 45 42 12 147 33 101 36
Fragments arranged by size:
252 235 155 147 142 132 101 51
45 42 36 33 21 15 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 397 733 750 1261 1419
Size: 397 336 17 511 158
Fragments arranged by size:
511 397 336 158 17
EcoO109I rG'GnC_Cy
Cuts at: 0 113 516 1023 1419
Size: 113 403 507 396
Fragments arranged by size:
507 403 396 113
FspI TGC'GCA
Cuts at: 0 557 1419
Size: 557 862
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 53 85 765 797 1027 1059 1419
Size: 53 32 680 32 230 32 360
Fragments arranged by size:
680 360 230 53 32 32 32
KpnI G_GTAC'C
Cuts at: 0 459 1419
Size: 459 960
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 659 986 1419
Size: 659 327 433
Fragments arranged by size:
659 433 327
MscI TGG'CCA
Cuts at: 0 697 1304 1419
Size: 697 607 115
Fragments arranged by size:
697 607 115
MspA1I CmG'CkG
Cuts at: 0 468 1113 1155 1419
Size: 468 645 42 264
Fragments arranged by size:
645 468 264 42
NspI r_CATG'y
Cuts at: 0 1110 1419
Size: 1110 309
PflMI CCAn_nnn'nTGG
Cuts at: 0 1026 1419
Size: 1026 393
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 904 936 1419
Size: 904 32 483
Fragments arranged by size:
904 483 32
PpuMI rG'GwC_Cy
Cuts at: 0 113 516 1419
Size: 113 403 903
Fragments arranged by size:
903 403 113
PshAI GACnn'nnGTC
Cuts at: 0 491 1419
Size: 491 928
PstI C_TGCA'G
Cuts at: 0 13 1043 1419
Size: 13 1030 376
Fragments arranged by size:
1030 376 13
PvuII CAG'CTG
Cuts at: 0 1113 1155 1419
Size: 1113 42 264
Fragments arranged by size:
1113 264 42
SacI G_AGCT'C
Cuts at: 0 1007 1390 1417 1419
Size: 1007 383 27 2
Fragments arranged by size:
1007 383 27 2
SexAI A'CCwGG_T
Cuts at: 0 916 1419
Size: 916 503
SfcI C'TryA_G
Cuts at: 0 9 489 1039 1419
Size: 9 480 550 380
Fragments arranged by size:
550 480 380 9
SmlI C'TyrA_G
Cuts at: 0 1375 1419
Size: 1375 44
SphI G_CATG'C
Cuts at: 0 1110 1419
Size: 1110 309
StuI AGG'CCT
Cuts at: 0 728 1419
Size: 728 691
StyI C'CwwG_G
Cuts at: 0 267 1419
Size: 267 1152
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 283 949 1419
Size: 283 666 470
Fragments arranged by size:
666 470 283
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1270 1302 1419
Size: 1270 32 117
Fragments arranged by size:
1270 117 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 429 705 969 1132 1297 1419
Size: 429 276 264 163 165 122
Fragments arranged by size:
429 276 264 165 163 122
ZraI GAC'GTC
Cuts at: 0 657 1419
Size: 657 762
Enzymes that do cut:
AarI AatII Acc65I AcuI AlfI AlwNI BanI BanII
BbsI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI
BsrDI BsrFI BssSI BstEII BstYI Bsu36I BtsI EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI Hin4I
KpnI MmeI MscI MspA1I NspI PflMI PpiI PpuMI
PshAI PstI PvuII SacI SexAI SfcI SmlI SphI
StuI StyI TaqII TstI XcmI ZraI
Enzymes that do not cut:
AccI AclI AfeI AflII AflIII AgeI AhdI AleI
AloI ApaI ApaLI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BclI
BfrBI BmgBI BmtI BsaAI BsiEI BsiWI BspHI BsrBI
BsrGI BssHII BstAPI BstBI BstXI BstZ17I BtgI BtgZI
ClaI CspCI DraI DraIII DrdI EagI EcoRI EcoRV
FalI FseI FspAI HaeII HincII HindIII HpaI KasI
MfeI MluI MslI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PfoI
PmeI PmlI PsiI PspOMI PspXI PsrI PvuI RsrII
SacII SalI SanDI SapI SbfI ScaI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI
TatI Tth111I XbaI XhoI XmaI XmnI