Restriction map for
pFN21AE4452
(Linear) (Six-base) MAPSORT of: KIEE4452.seq Check: 2273 from: 1 to: 1410
KIEE4452.orf
With 182 enzymes: SgfI *
March 22, 2012 18:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1003 1410
Size: 1003 407
AatII G_ACGT'C
Cuts at: 0 151 533 1410
Size: 151 382 877
Fragments arranged by size:
877 382 151
AccI GT'mk_AC
Cuts at: 0 527 1410
Size: 527 883
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 745 890 1410
Size: 745 145 520
Fragments arranged by size:
745 520 145
AflII C'TTAA_G
Cuts at: 0 715 1410
Size: 715 695
AflIII A'CryG_T
Cuts at: 0 791 1410
Size: 791 619
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 694 726 758 790 1410
Size: 694 32 32 32 620
Fragments arranged by size:
694 620 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1170 1410
Size: 1170 240
ApaI G_GGCC'C
Cuts at: 0 63 1410
Size: 63 1347
AvaI C'yCGr_G
Cuts at: 0 63 1410
Size: 63 1347
BanI G'GyrC_C
Cuts at: 0 488 1124 1410
Size: 488 636 286
Fragments arranged by size:
636 488 286
BanII G_rGCy'C
Cuts at: 0 63 94 133 344 461 837 1410
Size: 63 31 39 211 117 376 573
Fragments arranged by size:
573 376 211 117 63 39 31
BbeI G_GCGC'C
Cuts at: 0 492 1410
Size: 492 918
BbsI GAAGACnn'nnnn_
Cuts at: 0 1336 1410
Size: 1336 74
BbvCI CC'TCA_GC
Cuts at: 0 875 1410
Size: 875 535
BclI T'GATC_A
Cuts at: 0 1340 1410
Size: 1340 70
BglI GCCn_nnn'nGGC
Cuts at: 0 1075 1410
Size: 1075 335
BlpI GC'TnA_GC
Cuts at: 0 88 755 1410
Size: 88 667 655
Fragments arranged by size:
667 655 88
Bme1580I G_kGCm'C
Cuts at: 0 63 1021 1410
Size: 63 958 389
Fragments arranged by size:
958 389 63
BmgBI CAC'GTC
Cuts at: 0 176 642 1410
Size: 176 466 768
Fragments arranged by size:
768 466 176
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 120 406 694 1129 1246 1410
Size: 120 286 288 435 117 164
Fragments arranged by size:
435 288 286 164 120 117
Bpu10I CC'TnA_GC
Cuts at: 0 875 1298 1410
Size: 875 423 112
Fragments arranged by size:
875 423 112
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 412 553 997 1114 1410
Size: 412 141 444 117 296
Fragments arranged by size:
444 412 296 141 117
BsaI GGTCTCn'nnnn_
Cuts at: 0 838 1410
Size: 838 572
BsaHI Gr'CG_yC
Cuts at: 0 148 489 530 935 1007 1410
Size: 148 341 41 405 72 403
Fragments arranged by size:
405 403 341 148 72 41
BsaWI w'CCGG_w
Cuts at: 0 1214 1410
Size: 1214 196
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 580 610 694 724 1410
Size: 580 30 84 30 686
Fragments arranged by size:
686 580 84 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 123 231 481 484 601 715 748 892
Size: 123 108 250 3 117 114 33 144
Cuts at: 892 1126 1249 1410
Size: 234 123 161
Fragments arranged by size:
250 234 161 144 123 123 117 114
108 33 3
BseYI C'CCAG_C
Cuts at: 0 219 1410
Size: 219 1191
BsiHKAI G_wGCw'C
Cuts at: 0 94 344 461 506 1409 1410
Size: 94 250 117 45 903 1
Fragments arranged by size:
903 250 117 94 45 1
Bsp1286I G_dGCh'C
Cuts at: 0 63 94 133 344 461 506 837 1021
Size: 63 31 39 211 117 45 331 184
Cuts at: 1021 1409 1410
Size: 388 1
Fragments arranged by size:
388 331 211 184 117 63 45 39
31 1
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 554 566 762 1003 1178 1410
Size: 554 12 196 241 175 232
Fragments arranged by size:
554 241 232 196 175 12
BsrBI CCG'CTC
Cuts at: 0 75 637 1410
Size: 75 562 773
Fragments arranged by size:
773 562 75
BsrDI GCAATG_nn'
Cuts at: 0 1097 1410
Size: 1097 313
BsrFI r'CCGG_y
Cuts at: 0 215 422 1410
Size: 215 207 988
Fragments arranged by size:
988 215 207
BssHII G'CGCG_C
Cuts at: 0 516 518 520 1410
Size: 516 2 2 890
Fragments arranged by size:
890 516 2 2
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1404 1410
Size: 1404 6
BstXI CCAn_nnnn'nTGG
Cuts at: 0 219 1410
Size: 219 1191
BstYI r'GATC_y
Cuts at: 0 737 980 1410
Size: 737 243 430
Fragments arranged by size:
737 430 243
Bsu36I CC'TnA_GG
Cuts at: 0 820 1410
Size: 820 590
BtgI C'CryG_G
Cuts at: 0 106 1410
Size: 106 1304
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 834 1410
Size: 834 576
BtsI GCAGTG_nn'
Cuts at: 0 809 1410
Size: 809 601
EaeI y'GGCC_r
Cuts at: 0 278 452 1410
Size: 278 174 958
Fragments arranged by size:
958 278 174
EarI CTCTTCn'nnn_
Cuts at: 0 725 1410
Size: 725 685
EcoICRI GAG'CTC
Cuts at: 0 92 342 459 1410
Size: 92 250 117 951
Fragments arranged by size:
951 250 117 92
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 120 406 694 745 890 1129 1246 1410
Size: 120 286 288 51 145 239 117 164
Fragments arranged by size:
288 286 239 164 145 120 117 51
EcoNI CCTnn'n_nnAGG
Cuts at: 0 910 943 1111 1410
Size: 910 33 168 299
Fragments arranged by size:
910 299 168 33
EcoO109I rG'GnC_Cy
Cuts at: 0 59 60 192 1167 1410
Size: 59 1 132 975 243
Fragments arranged by size:
975 243 132 59 1
EcoRV GAT'ATC
Cuts at: 0 828 1410
Size: 828 582
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 396 1410
Size: 396 1014
HaeII r_GCGC'y
Cuts at: 0 406 492 1410
Size: 406 86 918
Fragments arranged by size:
918 406 86
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 965 997 1410
Size: 965 32 413
Fragments arranged by size:
965 413 32
HincII GTy'rAC
Cuts at: 0 528 1410
Size: 528 882
KasI G'GCGC_C
Cuts at: 0 488 1410
Size: 488 922
MspA1I CmG'CkG
Cuts at: 0 87 108 564 1410
Size: 87 21 456 846
Fragments arranged by size:
846 456 87 21
NaeI GCC'GGC
Cuts at: 0 217 1410
Size: 217 1193
NarI GG'CG_CC
Cuts at: 0 489 1410
Size: 489 921
NgoMIV G'CCGG_C
Cuts at: 0 215 1410
Size: 215 1195
NspI r_CATG'y
Cuts at: 0 795 1410
Size: 795 615
PciI A'CATG_T
Cuts at: 0 791 1410
Size: 791 619
PfoI T'CCnGG_A
Cuts at: 0 383 1410
Size: 383 1027
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 694 726 1410
Size: 694 32 684
Fragments arranged by size:
694 684 32
PpuMI rG'GwC_Cy
Cuts at: 0 1167 1410
Size: 1167 243
PspOMI G'GGCC_C
Cuts at: 0 59 1410
Size: 59 1351
PstI C_TGCA'G
Cuts at: 0 338 563 584 1410
Size: 338 225 21 826
Fragments arranged by size:
826 338 225 21
SacI G_AGCT'C
Cuts at: 0 94 344 461 1410
Size: 94 250 117 949
Fragments arranged by size:
949 250 117 94
SacII CC_GC'GG
Cuts at: 0 109 1410
Size: 109 1301
SalI G'TCGA_C
Cuts at: 0 526 1410
Size: 526 884
SexAI A'CCwGG_T
Cuts at: 0 161 1410
Size: 161 1249
SfcI C'TryA_G
Cuts at: 0 334 559 580 1410
Size: 334 225 21 830
Fragments arranged by size:
830 334 225 21
SfoI GGC'GCC
Cuts at: 0 490 1410
Size: 490 920
SmaI CCC'GGG
Cuts at: 0 65 1410
Size: 65 1345
SmlI C'TyrA_G
Cuts at: 0 427 568 715 1012 1129 1410
Size: 427 141 147 297 117 281
Fragments arranged by size:
427 297 281 147 141 117
StuI AGG'CCT
Cuts at: 0 10 1410
Size: 10 1400
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1076 1410
Size: 1076 334
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 324 1410
Size: 324 1086
XmaI C'CCGG_G
Cuts at: 0 63 1410
Size: 63 1347
ZraI GAC'GTC
Cuts at: 0 149 531 1410
Size: 149 382 879
Fragments arranged by size:
879 382 149
Enzymes that do cut:
AarI AatII AccI AcuI AflII AflIII AloI AlwNI
ApaI AvaI BanI BanII BbeI BbsI BbvCI BclI
BglI BlpI Bme1580I BmgBI BpmI Bpu10I BpuEI BsaI
BsaHI BsaWI BsaXI BseRI BseYI BsiHKAI Bsp1286I BspMI
BsrBI BsrDI BsrFI BssHII BstAPI BstXI BstYI Bsu36I
BtgI BtgZI BtsI EaeI EarI EcoICRI Eco57MI EcoNI
EcoO109I EcoRV FalI HaeII Hin4I HincII KasI MspA1I
NaeI NarI NgoMIV NspI PciI PfoI PpiI PpuMI
PspOMI PstI SacI SacII SalI SexAI SfcI SfoI
SmaI SmlI StuI TaqII XcmI XmaI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AgeI AhdI AleI AlfI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BcgI
BciVI BfrBI BglII BmrI BmtI BplI BsaAI BsaBI
BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrGI
BssSI BstBI BstEII BstZ17I ClaI CspCI DraI DraIII
DrdI EagI EciI EcoRI FseI FspI FspAI HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PmeI PmlI PshAI PsiI PspXI PsrI PvuI PvuII
RsrII SanDI SapI SbfI ScaI SfiI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI StyI SwaI TatI
TstI Tth111I XbaI XhoI XmnI