Restriction map for
pFN21AE4447
(Linear) (Six-base) MAPSORT of: KIEE4447.seq Check: 3713 from: 1 to: 1386
KIEE4447.orf
With 182 enzymes: SgfI *
March 22, 2012 18:03 ..
AatII G_ACGT'C
Cuts at: 0 1192 1386
Size: 1192 194
AccI GT'mk_AC
Cuts at: 0 605 1386
Size: 605 781
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 617 1244 1386
Size: 617 627 142
Fragments arranged by size:
627 617 142
AflIII A'CryG_T
Cuts at: 0 290 395 1386
Size: 290 105 991
Fragments arranged by size:
991 290 105
AhdI GACnn_n'nnGTC
Cuts at: 0 1281 1386
Size: 1281 105
AleI CACnn'nnGTG
Cuts at: 0 220 886 1386
Size: 220 666 500
Fragments arranged by size:
666 500 220
ApaI G_GGCC'C
Cuts at: 0 341 1386
Size: 341 1045
ApoI r'AATT_y
Cuts at: 0 568 1386
Size: 568 818
AvaI C'yCGr_G
Cuts at: 0 196 1386
Size: 196 1190
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1245 1278 1386
Size: 1245 33 108
Fragments arranged by size:
1245 108 33
BanI G'GyrC_C
Cuts at: 0 475 1386
Size: 475 911
BanII G_rGCy'C
Cuts at: 0 167 341 1386
Size: 167 174 1045
Fragments arranged by size:
1045 174 167
BbsI GAAGACnn'nnnn_
Cuts at: 0 984 1237 1386
Size: 984 253 149
Fragments arranged by size:
984 253 149
BbvCI CC'TCA_GC
Cuts at: 0 907 1386
Size: 907 479
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1172 1206 1386
Size: 1172 34 180
Fragments arranged by size:
1172 180 34
BclI T'GATC_A
Cuts at: 0 32 1386
Size: 32 1354
BglII A'GATC_T
Cuts at: 0 380 1386
Size: 380 1006
Bme1580I G_kGCm'C
Cuts at: 0 341 520 700 1386
Size: 341 179 180 686
Fragments arranged by size:
686 341 180 179
BmgBI CAC'GTC
Cuts at: 0 291 1386
Size: 291 1095
BmrI ACTGGGnnnn_n'
Cuts at: 0 1145 1294 1341 1386
Size: 1145 149 47 45
Fragments arranged by size:
1145 149 47 45
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 484 642 1386
Size: 484 158 744
Fragments arranged by size:
744 484 158
Bpu10I CC'TnA_GC
Cuts at: 0 907 1386
Size: 907 479
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 550 1386
Size: 550 836
BsaI GGTCTCn'nnnn_
Cuts at: 0 115 1386
Size: 115 1271
BsaHI Gr'CG_yC
Cuts at: 0 1189 1386
Size: 1189 197
BseYI C'CCAG_C
Cuts at: 0 201 858 1043 1179 1386
Size: 201 657 185 136 207
Fragments arranged by size:
657 207 201 185 136
Bsp1286I G_dGCh'C
Cuts at: 0 167 341 520 700 1386
Size: 167 174 179 180 686
Fragments arranged by size:
686 180 179 174 167
BspHI T'CATG_A
Cuts at: 0 29 737 1386
Size: 29 708 649
Fragments arranged by size:
708 649 29
BsrFI r'CCGG_y
Cuts at: 0 1356 1386
Size: 1356 30
BstEII G'GTnAC_C
Cuts at: 0 879 1386
Size: 879 507
BstXI CCAn_nnnn'nTGG
Cuts at: 0 156 636 957 1386
Size: 156 480 321 429
Fragments arranged by size:
480 429 321 156
BstYI r'GATC_y
Cuts at: 0 152 380 1386
Size: 152 228 1006
Fragments arranged by size:
1006 228 152
BtgI C'CryG_G
Cuts at: 0 884 956 1016 1386
Size: 884 72 60 370
Fragments arranged by size:
884 370 72 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 408 1386
Size: 408 978
DrdI GACnn_nn'nnGTC
Cuts at: 0 238 1386
Size: 238 1148
EarI CTCTTCn'nnn_
Cuts at: 0 1220 1386
Size: 1220 166
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 661 1386
Size: 661 725
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 484 617 642 1244 1386
Size: 484 133 25 602 142
Fragments arranged by size:
602 484 142 133 25
EcoO109I rG'GnC_Cy
Cuts at: 0 337 1386
Size: 337 1049
FspI TGC'GCA
Cuts at: 0 551 1386
Size: 551 835
FspAI rTGC'GCAy
Cuts at: 0 551 1386
Size: 551 835
HaeII r_GCGC'y
Cuts at: 0 351 920 1003 1386
Size: 351 569 83 383
Fragments arranged by size:
569 383 351 83
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 197 229 1267 1299 1386
Size: 197 32 1038 32 87
Fragments arranged by size:
1038 197 87 32 32
HincII GTy'rAC
Cuts at: 0 1118 1386
Size: 1118 268
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 287 324 1386
Size: 287 37 1062
Fragments arranged by size:
1062 287 37
MslI CAynn'nnrTG
Cuts at: 0 220 271 289 736 886 955 973 1386
Size: 220 51 18 447 150 69 18 413
Fragments arranged by size:
447 413 220 150 69 51 18 18
NcoI C'CATG_G
Cuts at: 0 956 1386
Size: 956 430
PflMI CCAn_nnn'nTGG
Cuts at: 0 681 962 1386
Size: 681 281 424
Fragments arranged by size:
681 424 281
PspOMI G'GGCC_C
Cuts at: 0 337 1386
Size: 337 1049
PstI C_TGCA'G
Cuts at: 0 513 1386
Size: 513 873
SfcI C'TryA_G
Cuts at: 0 509 1386
Size: 509 877
SmaI CCC'GGG
Cuts at: 0 198 1386
Size: 198 1188
SmlI C'TyrA_G
Cuts at: 0 529 1386
Size: 529 857
SrfI GCCC'GGGC
Cuts at: 0 198 1386
Size: 198 1188
StyI C'CwwG_G
Cuts at: 0 956 1067 1386
Size: 956 111 319
Fragments arranged by size:
956 319 111
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 244 1386
Size: 244 1142
TatI w'GTAC_w
Cuts at: 0 278 1386
Size: 278 1108
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 633 636 1386
Size: 633 3 750
Fragments arranged by size:
750 633 3
XmaI C'CCGG_G
Cuts at: 0 196 1386
Size: 196 1190
XmnI GAAnn'nnTTC
Cuts at: 0 323 1386
Size: 323 1063
ZraI GAC'GTC
Cuts at: 0 1190 1386
Size: 1190 196
Enzymes that do cut:
AatII AccI AcuI AflIII AhdI AleI ApaI ApoI
AvaI BaeI BanI BanII BbsI BbvCI BcgI BclI
BglII Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI
BsaHI BseYI Bsp1286I BspHI BsrFI BstEII BstXI BstYI
BtgI BtgZI DrdI EarI EciI Eco57MI EcoO109I FspI
FspAI HaeII Hin4I HincII MmeI MslI NcoI PflMI
PspOMI PstI SfcI SmaI SmlI SrfI StyI TaqII
TatI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AgeI AlfI AloI
AlwNI ApaLI AscI AseI AsiSI AvrII BamHI BbeI
BciVI BfrBI BglI BlpI BmtI BplI BsaAI BsaBI
BsaWI BsaXI BseRI BsgI BsiEI BsiHKAI BsiWI BsmI
BsmBI BspEI BspMI BsrBI BsrDI BsrGI BssHII BssSI
BstAPI BstBI BstZ17I Bsu36I BtsI ClaI CspCI DraI
DraIII EaeI EagI EcoICRI EcoNI EcoRI EcoRV FalI
FseI HindIII HpaI KasI KpnI MfeI MluI MscI
MspA1I NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspXI PsrI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SspI StuI SwaI TstI Tth111I XbaI XhoI