Restriction map for
pFN21AE4445
(Linear) (Six-base) MAPSORT of: KIEE4445.seq Check: 7606 from: 1 to: 1383
KIEE4445.orf
With 182 enzymes: SgfI *
March 22, 2012 18:03 ..
AccI GT'mk_AC
Cuts at: 0 1147 1383
Size: 1147 236
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 270 1294 1383
Size: 270 1024 89
Fragments arranged by size:
1024 270 89
AfeI AGC'GCT
Cuts at: 0 256 1383
Size: 256 1127
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 332 1383
Size: 332 1051
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 430 462 1010 1042 1383
Size: 430 32 548 32 341
Fragments arranged by size:
548 430 341 32 32
ApaI G_GGCC'C
Cuts at: 0 78 1383
Size: 78 1305
ApaLI G'TGCA_C
Cuts at: 0 870 1383
Size: 870 513
ApoI r'AATT_y
Cuts at: 0 714 829 1259 1383
Size: 714 115 430 124
Fragments arranged by size:
714 430 124 115
BanI G'GyrC_C
Cuts at: 0 189 367 1383
Size: 189 178 1016
Fragments arranged by size:
1016 189 178
BanII G_rGCy'C
Cuts at: 0 78 1383
Size: 78 1305
BbeI G_GCGC'C
Cuts at: 0 371 1383
Size: 371 1012
BciVI GTATCCnnnnn_n'
Cuts at: 0 802 1383
Size: 802 581
BglII A'GATC_T
Cuts at: 0 1269 1383
Size: 1269 114
BlpI GC'TnA_GC
Cuts at: 0 1069 1383
Size: 1069 314
Bme1580I G_kGCm'C
Cuts at: 0 78 194 874 1383
Size: 78 116 680 509
Fragments arranged by size:
680 509 116 78
BmgBI CAC'GTC
Cuts at: 0 456 1383
Size: 456 927
BmrI ACTGGGnnnn_n'
Cuts at: 0 183 1314 1383
Size: 183 1131 69
Fragments arranged by size:
1131 183 69
BsaI GGTCTCn'nnnn_
Cuts at: 0 1306 1383
Size: 1306 77
BsaHI Gr'CG_yC
Cuts at: 0 368 1383
Size: 368 1015
BsaWI w'CCGG_w
Cuts at: 0 500 1383
Size: 500 883
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 430 460 691 721 1383
Size: 430 30 231 30 662
Fragments arranged by size:
662 430 231 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 352 1383
Size: 352 1031
BseYI C'CCAG_C
Cuts at: 0 77 1254 1383
Size: 77 1177 129
Fragments arranged by size:
1177 129 77
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1168 1383
Size: 1168 215
BsiEI CG_ry'CG
Cuts at: 0 13 59 127 244 1383
Size: 13 46 68 117 1139
Fragments arranged by size:
1139 117 68 46 13
BsiHKAI G_wGCw'C
Cuts at: 0 874 1383
Size: 874 509
Bsp1286I G_dGCh'C
Cuts at: 0 78 194 874 1383
Size: 78 116 680 509
Fragments arranged by size:
680 509 116 78
BspEI T'CCGG_A
Cuts at: 0 500 1383
Size: 500 883
BsrBI CCG'CTC
Cuts at: 0 55 1383
Size: 55 1328
BsrDI GCAATG_nn'
Cuts at: 0 1123 1383
Size: 1123 260
BsrFI r'CCGG_y
Cuts at: 0 12 132 1383
Size: 12 120 1251
Fragments arranged by size:
1251 120 12
BssHII G'CGCG_C
Cuts at: 0 319 1383
Size: 319 1064
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1351 1383
Size: 1351 32
BstXI CCAn_nnnn'nTGG
Cuts at: 0 456 1383
Size: 456 927
BstYI r'GATC_y
Cuts at: 0 1269 1287 1383
Size: 1269 18 96
Fragments arranged by size:
1269 96 18
BstZ17I GTA'TAC
Cuts at: 0 1148 1383
Size: 1148 235
BtgI C'CryG_G
Cuts at: 0 362 801 1105 1383
Size: 362 439 304 278
Fragments arranged by size:
439 362 304 278
BtsI GCAGTG_nn'
Cuts at: 0 314 1383
Size: 314 1069
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 827 862 1383
Size: 827 35 521
Fragments arranged by size:
827 521 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 745 1383
Size: 745 638
EaeI y'GGCC_r
Cuts at: 0 10 56 106 124 241 418 1189 1383
Size: 10 46 50 18 117 177 771 194
Fragments arranged by size:
771 194 177 117 50 46 18 10
EagI C'GGCC_G
Cuts at: 0 10 56 124 241 1383
Size: 10 46 68 117 1142
Fragments arranged by size:
1142 117 68 46 10
EarI CTCTTCn'nnn_
Cuts at: 0 49 358 499 1309 1383
Size: 49 309 141 810 74
Fragments arranged by size:
810 309 141 74 49
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 270 1294 1383
Size: 270 1024 89
Fragments arranged by size:
1024 270 89
EcoNI CCTnn'n_nnAGG
Cuts at: 0 355 1383
Size: 355 1028
EcoO109I rG'GnC_Cy
Cuts at: 0 19 460 1383
Size: 19 441 923
Fragments arranged by size:
923 441 19
EcoRI G'AATT_C
Cuts at: 0 1259 1383
Size: 1259 124
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1063 1383
Size: 1063 320
HaeII r_GCGC'y
Cuts at: 0 258 371 1383
Size: 258 113 1012
Fragments arranged by size:
1012 258 113
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 381 413 1383
Size: 381 32 970
Fragments arranged by size:
970 381 32
HincII GTy'rAC
Cuts at: 0 642 1383
Size: 642 741
HpaI GTT'AAC
Cuts at: 0 642 1383
Size: 642 741
KasI G'GCGC_C
Cuts at: 0 367 1383
Size: 367 1016
MscI TGG'CCA
Cuts at: 0 1191 1383
Size: 1191 192
MslI CAynn'nnrTG
Cuts at: 0 1140 1383
Size: 1140 243
MspA1I CmG'CkG
Cuts at: 0 129 364 1383
Size: 129 235 1019
Fragments arranged by size:
1019 235 129
NaeI GCC'GGC
Cuts at: 0 14 134 1383
Size: 14 120 1249
Fragments arranged by size:
1249 120 14
NarI GG'CG_CC
Cuts at: 0 368 1383
Size: 368 1015
NcoI C'CATG_G
Cuts at: 0 801 1105 1383
Size: 801 304 278
Fragments arranged by size:
801 304 278
NgoMIV G'CCGG_C
Cuts at: 0 12 132 1383
Size: 12 120 1251
Fragments arranged by size:
1251 120 12
NotI GC'GGCC_GC
Cuts at: 0 241 1383
Size: 241 1142
PflMI CCAn_nnn'nTGG
Cuts at: 0 1106 1198 1383
Size: 1106 92 185
Fragments arranged by size:
1106 185 92
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 430 462 1383
Size: 430 32 921
Fragments arranged by size:
921 430 32
PpuMI rG'GwC_Cy
Cuts at: 0 19 1383
Size: 19 1364
PspOMI G'GGCC_C
Cuts at: 0 74 1383
Size: 74 1309
RsrII CG'GwC_CG
Cuts at: 0 304 1383
Size: 304 1079
SacII CC_GC'GG
Cuts at: 0 365 1383
Size: 365 1018
SanDI GG'GwC_CC
Cuts at: 0 19 1383
Size: 19 1364
SapI GCTCTTCn'nnn_
Cuts at: 0 49 1383
Size: 49 1334
SfoI GGC'GCC
Cuts at: 0 369 1383
Size: 369 1014
StyI C'CwwG_G
Cuts at: 0 801 1105 1383
Size: 801 304 278
Fragments arranged by size:
801 304 278
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 788 1383
Size: 788 595
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 857 1383
Size: 857 526
XmnI GAAnn'nnTTC
Cuts at: 0 959 1046 1383
Size: 959 87 337
Fragments arranged by size:
959 337 87
Enzymes that do cut:
AccI AcuI AfeI AlfI AloI ApaI ApaLI ApoI
BanI BanII BbeI BciVI BglII BlpI Bme1580I BmgBI
BmrI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BsrBI BsrDI BsrFI BssHII
BstAPI BstXI BstYI BstZ17I BtgI BtsI CspCI DrdI
EaeI EagI EarI Eco57MI EcoNI EcoO109I EcoRI FalI
HaeII Hin4I HincII HpaI KasI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NotI PflMI PpiI PpuMI
PspOMI RsrII SacII SanDI SapI SfoI StyI TaqII
XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AflIII AgeI AhdI
AleI AlwNI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BbsI BbvCI BcgI BclI BfrBI BglI BmtI
BplI BpmI Bpu10I BpuEI BsaAI BsaBI BsiWI BsmI
BsmBI BspHI BspMI BsrGI BssSI BstBI BstEII Bsu36I
BtgZI ClaI DraI DraIII EciI EcoICRI EcoRV FseI
FspI FspAI HindIII KpnI MfeI MluI MmeI NdeI
NheI NruI NsiI NspI PacI PasI PciI PfoI
PmeI PmlI PshAI PsiI PspXI PsrI PstI PvuI
PvuII SacI SalI SbfI ScaI SexAI SfcI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI TatI TstI Tth111I XbaI XhoI
XmaI ZraI