Restriction map for
pFN21AE4443
(Linear) (Six-base) MAPSORT of: KIEE4443.seq Check: 6283 from: 1 to: 1377
KIEE4443.orf
With 182 enzymes: SgfI *
March 22, 2012 18:02 ..
AccI GT'mk_AC
Cuts at: 0 1029 1377
Size: 1029 348
Acc65I G'GTAC_C
Cuts at: 0 1240 1377
Size: 1240 137
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 497 1147 1377
Size: 497 650 230
Fragments arranged by size:
650 497 230
AleI CACnn'nnGTG
Cuts at: 0 559 1377
Size: 559 818
AlwNI CAG_nnn'CTG
Cuts at: 0 1125 1288 1377
Size: 1125 163 89
Fragments arranged by size:
1125 163 89
ApaLI G'TGCA_C
Cuts at: 0 700 1377
Size: 700 677
ApoI r'AATT_y
Cuts at: 0 760 1377
Size: 760 617
AvaI C'yCGr_G
Cuts at: 0 1229 1377
Size: 1229 148
BanI G'GyrC_C
Cuts at: 0 1240 1377
Size: 1240 137
BanII G_rGCy'C
Cuts at: 0 743 1377
Size: 743 634
BbvCI CC'TCA_GC
Cuts at: 0 1090 1377
Size: 1090 287
BclI T'GATC_A
Cuts at: 0 2 630 1377
Size: 2 628 747
Fragments arranged by size:
747 628 2
BglI GCCn_nnn'nGGC
Cuts at: 0 269 1377
Size: 269 1108
BglII A'GATC_T
Cuts at: 0 890 1377
Size: 890 487
Bme1580I G_kGCm'C
Cuts at: 0 228 704 1073 1170 1377
Size: 228 476 369 97 207
Fragments arranged by size:
476 369 228 207 97
BmrI ACTGGGnnnn_n'
Cuts at: 0 189 1377
Size: 189 1188
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 190 1377
Size: 37 153 1187
Fragments arranged by size:
1187 153 37
Bpu10I CC'TnA_GC
Cuts at: 0 809 1090 1377
Size: 809 281 287
Fragments arranged by size:
809 287 281
BsaAI yAC'GTr
Cuts at: 0 1108 1377
Size: 1108 269
BsaWI w'CCGG_w
Cuts at: 0 458 1377
Size: 458 919
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 880 1330 1377
Size: 880 450 47
Fragments arranged by size:
880 450 47
BseYI C'CCAG_C
Cuts at: 0 228 1377
Size: 228 1149
BsiHKAI G_wGCw'C
Cuts at: 0 704 1377
Size: 704 673
BsmBI CGTCTCn'nnnn_
Cuts at: 0 650 1377
Size: 650 727
Bsp1286I G_dGCh'C
Cuts at: 0 228 704 743 1073 1170 1377
Size: 228 476 39 330 97 207
Fragments arranged by size:
476 330 228 207 97 39
BspHI T'CATG_A
Cuts at: 0 764 1377
Size: 764 613
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1338 1377
Size: 1338 39
BstYI r'GATC_y
Cuts at: 0 890 1278 1377
Size: 890 388 99
Fragments arranged by size:
890 388 99
BtgI C'CryG_G
Cuts at: 0 26 265 735 1377
Size: 26 239 470 642
Fragments arranged by size:
642 470 239 26
BtsI GCAGTG_nn'
Cuts at: 0 524 702 1071 1377
Size: 524 178 369 306
Fragments arranged by size:
524 369 306 178
DraIII CAC_nnn'GTG
Cuts at: 0 602 1377
Size: 602 775
EaeI y'GGCC_r
Cuts at: 0 732 745 1377
Size: 732 13 632
Fragments arranged by size:
732 632 13
EarI CTCTTCn'nnn_
Cuts at: 0 439 473 524 1377
Size: 439 34 51 853
Fragments arranged by size:
853 439 51 34
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1341 1377
Size: 1341 36
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 190 497 1147 1377
Size: 37 153 307 650 230
Fragments arranged by size:
650 307 230 153 37
EcoNI CCTnn'n_nnAGG
Cuts at: 0 808 1377
Size: 808 569
EcoO109I rG'GnC_Cy
Cuts at: 0 116 137 144 254 771 1122 1377
Size: 116 21 7 110 517 351 255
Fragments arranged by size:
517 351 255 116 110 21 7
EcoRI G'AATT_C
Cuts at: 0 760 1377
Size: 760 617
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 384 1377
Size: 384 993
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 898 930 1377
Size: 898 32 447
Fragments arranged by size:
898 447 32
HincII GTy'rAC
Cuts at: 0 591 789 1030 1377
Size: 591 198 241 347
Fragments arranged by size:
591 347 241 198
HindIII A'AGCT_T
Cuts at: 0 274 1294 1377
Size: 274 1020 83
Fragments arranged by size:
1020 274 83
KpnI G_GTAC'C
Cuts at: 0 1244 1377
Size: 1244 133
MslI CAynn'nnrTG
Cuts at: 0 559 1377
Size: 559 818
MspA1I CmG'CkG
Cuts at: 0 783 1377
Size: 783 594
NcoI C'CATG_G
Cuts at: 0 265 735 1377
Size: 265 470 642
Fragments arranged by size:
642 470 265
NspI r_CATG'y
Cuts at: 0 517 1119 1377
Size: 517 602 258
Fragments arranged by size:
602 517 258
PasI CC'CwG_GG
Cuts at: 0 1040 1066 1377
Size: 1040 26 311
Fragments arranged by size:
1040 311 26
PflMI CCAn_nnn'nTGG
Cuts at: 0 577 1377
Size: 577 800
PfoI T'CCnGG_A
Cuts at: 0 14 125 860 1377
Size: 14 111 735 517
Fragments arranged by size:
735 517 111 14
PpuMI rG'GwC_Cy
Cuts at: 0 1122 1377
Size: 1122 255
SalI G'TCGA_C
Cuts at: 0 1028 1377
Size: 1028 349
ScaI AGT'ACT
Cuts at: 0 217 1377
Size: 217 1160
SexAI A'CCwGG_T
Cuts at: 0 570 1377
Size: 570 807
SfcI C'TryA_G
Cuts at: 0 995 1377
Size: 995 382
SnaBI TAC'GTA
Cuts at: 0 1108 1377
Size: 1108 269
SphI G_CATG'C
Cuts at: 0 517 1377
Size: 517 860
StyI C'CwwG_G
Cuts at: 0 133 265 735 1377
Size: 133 132 470 642
Fragments arranged by size:
642 470 133 132
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 101 1377
Size: 101 1276
TatI w'GTAC_w
Cuts at: 0 215 713 1377
Size: 215 498 664
Fragments arranged by size:
664 498 215
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 337 369 1160 1192 1377
Size: 337 32 791 32 185
Fragments arranged by size:
791 337 185 32 32
XmnI GAAnn'nnTTC
Cuts at: 0 61 1377
Size: 61 1316
Enzymes that do cut:
AccI Acc65I AcuI AleI AlwNI ApaLI ApoI AvaI
BanI BanII BbvCI BclI BglI BglII Bme1580I BmrI
BpmI Bpu10I BsaAI BsaWI BseRI BseYI BsiHKAI BsmBI
Bsp1286I BspHI BstAPI BstYI BtgI BtsI DraIII EaeI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI Hin4I
HincII HindIII KpnI MslI MspA1I NcoI NspI PasI
PflMI PfoI PpuMI SalI ScaI SexAI SfcI SnaBI
SphI StyI TaqII TatI TstI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AgeI AhdI
AlfI AloI ApaI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbsI BcgI BciVI BfrBI BlpI BmgBI
BmtI BplI BpuEI BsaI BsaBI BsaHI BsaXI BsgI
BsiEI BsiWI BsmI BspEI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DrdI EagI EcoICRI EcoRV
FseI FspI FspAI HaeII HpaI KasI MfeI MluI
MmeI MscI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PciI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII
SacI SacII SanDI SapI SbfI SfiI SfoI SgfI
SgrAI SmaI SmlI SpeI SrfI SspI StuI SwaI
Tth111I XbaI XcmI XhoI XmaI ZraI