Restriction map for
pFN21AE4441
(Linear) (Six-base) MAPSORT of: KIEE4441.seq Check: 9596 from: 1 to: 1374
KIEE4441.orf
With 182 enzymes: SgfI *
March 22, 2012 18:02 ..
AatII G_ACGT'C
Cuts at: 0 470 1374
Size: 470 904
AccI GT'mk_AC
Cuts at: 0 569 1374
Size: 569 805
AclI AA'CG_TT
Cuts at: 0 956 1374
Size: 956 418
AflIII A'CryG_T
Cuts at: 0 1212 1374
Size: 1212 162
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1142 1174 1374
Size: 1142 32 200
Fragments arranged by size:
1142 200 32
ApoI r'AATT_y
Cuts at: 0 1258 1374
Size: 1258 116
BamHI G'GATC_C
Cuts at: 0 296 514 1374
Size: 296 218 860
Fragments arranged by size:
860 296 218
BanI G'GyrC_C
Cuts at: 0 532 1374
Size: 532 842
BbsI GAAGACnn'nnnn_
Cuts at: 0 857 1374
Size: 857 517
BbvCI CC'TCA_GC
Cuts at: 0 48 1374
Size: 48 1326
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 479 513 1098 1132 1374
Size: 479 34 585 34 242
Fragments arranged by size:
585 479 242 34 34
BclI T'GATC_A
Cuts at: 0 554 1374
Size: 554 820
BglII A'GATC_T
Cuts at: 0 967 1374
Size: 967 407
Bpu10I CC'TnA_GC
Cuts at: 0 48 1374
Size: 48 1326
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1253 1374
Size: 1253 121
BsaAI yAC'GTr
Cuts at: 0 671 980 1374
Size: 671 309 394
Fragments arranged by size:
671 394 309
BsaHI Gr'CG_yC
Cuts at: 0 467 690 1374
Size: 467 223 684
Fragments arranged by size:
684 467 223
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 436 466 1142 1172 1374
Size: 436 30 676 30 202
Fragments arranged by size:
676 436 202 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 85 1099 1374
Size: 85 1014 275
Fragments arranged by size:
1014 275 85
BseYI C'CCAG_C
Cuts at: 0 188 1374
Size: 188 1186
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 441 1374
Size: 441 933
BsiEI CG_ry'CG
Cuts at: 0 369 489 1374
Size: 369 120 885
Fragments arranged by size:
885 369 120
BsmI GAATG_Cn'
Cuts at: 0 618 1374
Size: 618 756
BsmBI CGTCTCn'nnnn_
Cuts at: 0 681 1374
Size: 681 693
BspHI T'CATG_A
Cuts at: 0 908 1374
Size: 908 466
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 905 1374
Size: 905 469
BstBI TT'CG_AA
Cuts at: 0 374 1374
Size: 374 1000
BstYI r'GATC_y
Cuts at: 0 296 514 967 1374
Size: 296 218 453 407
Fragments arranged by size:
453 407 296 218
BtgI C'CryG_G
Cuts at: 0 461 536 989 1374
Size: 461 75 453 385
Fragments arranged by size:
461 453 385 75
EaeI y'GGCC_r
Cuts at: 0 366 1374
Size: 366 1008
EagI C'GGCC_G
Cuts at: 0 366 1374
Size: 366 1008
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 121 1374
Size: 121 1253
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1029 1374
Size: 1029 345
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1051 1083 1374
Size: 1051 32 291
Fragments arranged by size:
1051 291 32
HincII GTy'rAC
Cuts at: 0 543 570 1093 1374
Size: 543 27 523 281
Fragments arranged by size:
543 523 281 27
MslI CAynn'nnrTG
Cuts at: 0 149 1374
Size: 149 1225
MspA1I CmG'CkG
Cuts at: 0 219 1374
Size: 219 1155
NcoI C'CATG_G
Cuts at: 0 461 536 989 1374
Size: 461 75 453 385
Fragments arranged by size:
461 453 385 75
NspI r_CATG'y
Cuts at: 0 1216 1374
Size: 1216 158
PasI CC'CwG_GG
Cuts at: 0 998 1374
Size: 998 376
PciI A'CATG_T
Cuts at: 0 1212 1374
Size: 1212 162
PflMI CCAn_nnn'nTGG
Cuts at: 0 236 990 1374
Size: 236 754 384
Fragments arranged by size:
754 384 236
PfoI T'CCnGG_A
Cuts at: 0 517 1374
Size: 517 857
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1142 1174 1374
Size: 1142 32 200
Fragments arranged by size:
1142 200 32
PshAI GACnn'nnGTC
Cuts at: 0 394 1374
Size: 394 980
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 886 918 1256 1288 1374
Size: 886 32 338 32 86
Fragments arranged by size:
886 338 86 32 32
PstI C_TGCA'G
Cuts at: 0 1022 1374
Size: 1022 352
PvuII CAG'CTG
Cuts at: 0 219 1374
Size: 219 1155
SalI G'TCGA_C
Cuts at: 0 568 1374
Size: 568 806
SfcI C'TryA_G
Cuts at: 0 1018 1374
Size: 1018 356
SmlI C'TyrA_G
Cuts at: 0 1268 1374
Size: 1268 106
StyI C'CwwG_G
Cuts at: 0 33 111 392 461 536 989 1374
Size: 33 78 281 69 75 453 385
Fragments arranged by size:
453 385 281 78 75 69 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 503 798 1295 1374
Size: 503 295 497 79
Fragments arranged by size:
503 497 295 79
TatI w'GTAC_w
Cuts at: 0 650 1245 1374
Size: 650 595 129
Fragments arranged by size:
650 595 129
Tth111I GACn'n_nGTC
Cuts at: 0 924 1374
Size: 924 450
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 810 1374
Size: 810 564
XmnI GAAnn'nnTTC
Cuts at: 0 1157 1374
Size: 1157 217
ZraI GAC'GTC
Cuts at: 0 468 1374
Size: 468 906
Enzymes that do cut:
AatII AccI AclI AflIII AloI ApoI BamHI BanI
BbsI BbvCI BcgI BclI BglII Bpu10I BpuEI BsaAI
BsaHI BsaXI BseRI BseYI BsgI BsiEI BsmI BsmBI
BspHI BspMI BstBI BstYI BtgI EaeI EagI EciI
FalI Hin4I HincII MslI MspA1I NcoI NspI PasI
PciI PflMI PfoI PpiI PshAI PsrI PstI PvuII
SalI SfcI SmlI StyI TaqII TatI Tth111I XcmI
XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AcuI AfeI AflII AgeI AhdI AleI
AlfI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BanII BbeI BciVI BfrBI BglI BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI BsaI BsaBI
BsaWI BsiHKAI BsiWI Bsp1286I BspEI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstEII BstXI BstZ17I Bsu36I
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EarI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FseI FspI
FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PmeI PmlI PpuMI PsiI PspOMI
PspXI PvuI RsrII SacI SacII SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI TstI XbaI
XhoI XmaI