Restriction map for
pFN21AE4440
(Linear) (Six-base) MAPSORT of: KIEE4440.seq Check: 4502 from: 1 to: 1371
KIEE4440.orf
With 182 enzymes: SgfI *
March 22, 2012 18:02 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 247 1131 1371
Size: 247 884 240
Fragments arranged by size:
884 247 240
AleI CACnn'nnGTG
Cuts at: 0 754 1371
Size: 754 617
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 65 1371
Size: 65 1306
AlwNI CAG_nnn'CTG
Cuts at: 0 627 1299 1371
Size: 627 672 72
Fragments arranged by size:
672 627 72
ApaI G_GGCC'C
Cuts at: 0 1134 1156 1371
Size: 1134 22 215
Fragments arranged by size:
1134 215 22
ApoI r'AATT_y
Cuts at: 0 219 1371
Size: 219 1152
AvaI C'yCGr_G
Cuts at: 0 733 771 1133 1193 1243 1371
Size: 733 38 362 60 50 128
Fragments arranged by size:
733 362 128 60 50 38
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 77 110 1371
Size: 77 33 1261
Fragments arranged by size:
1261 77 33
BamHI G'GATC_C
Cuts at: 0 194 1371
Size: 194 1177
BanI G'GyrC_C
Cuts at: 0 256 1371
Size: 256 1115
BanII G_rGCy'C
Cuts at: 0 236 470 1134 1156 1371
Size: 236 234 664 22 215
Fragments arranged by size:
664 236 234 215 22
BbeI G_GCGC'C
Cuts at: 0 260 1371
Size: 260 1111
BbvCI CC'TCA_GC
Cuts at: 0 488 710 854 1371
Size: 488 222 144 517
Fragments arranged by size:
517 488 222 144
BciVI GTATCCnnnnn_n'
Cuts at: 0 78 1371
Size: 78 1293
BfrBI ATG'CAT
Cuts at: 0 104 1371
Size: 104 1267
BglI GCCn_nnn'nGGC
Cuts at: 0 666 1321 1371
Size: 666 655 50
Fragments arranged by size:
666 655 50
Bme1580I G_kGCm'C
Cuts at: 0 273 1134 1156 1371
Size: 273 861 22 215
Fragments arranged by size:
861 273 215 22
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 135 633 1011 1371
Size: 135 498 378 360
Fragments arranged by size:
498 378 360 135
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 539 737 994 1044 1093 1200 1210 1321
Size: 539 198 257 50 49 107 10 111
Cuts at: 1321 1351 1371
Size: 30 20
Fragments arranged by size:
539 257 198 111 107 50 49 30
20 10
Bpu10I CC'TnA_GC
Cuts at: 0 488 710 854 863 1371
Size: 488 222 144 9 508
Fragments arranged by size:
508 488 222 144 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 343 1228 1371
Size: 343 885 143
Fragments arranged by size:
885 343 143
BsaHI Gr'CG_yC
Cuts at: 0 257 1371
Size: 257 1114
BsaWI w'CCGG_w
Cuts at: 0 794 1371
Size: 794 577
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 537 567 1294 1324 1371
Size: 537 30 727 30 47
Fragments arranged by size:
727 537 47 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 197 535 538 541 544 547 700 703
Size: 197 338 3 3 3 3 153 3
Cuts at: 703 706 709 712 715 718 736 871 925
Size: 3 3 3 3 3 18 135 54
Cuts at: 925 928 931 934 997 1000 1099 1102 1105
Size: 3 3 3 63 3 99 3 3
Cuts at: 1105 1261 1273 1276 1371
Size: 156 12 3 95
Fragments arranged by size:
338 197 156 153 135 99 95 63
54 18 12 3 3 3 3 3
3 3 3 3 3 3 3 3
3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 139 568 838 899 1371
Size: 139 429 270 61 472
Fragments arranged by size:
472 429 270 139 61
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 674 1371
Size: 674 697
BsiEI CG_ry'CG
Cuts at: 0 814 1371
Size: 814 557
BsiHKAI G_wGCw'C
Cuts at: 0 236 896 1371
Size: 236 660 475
Fragments arranged by size:
660 475 236
Bsp1286I G_dGCh'C
Cuts at: 0 236 273 470 896 1134 1156 1371
Size: 236 37 197 426 238 22 215
Fragments arranged by size:
426 238 236 215 197 37 22
BspEI T'CCGG_A
Cuts at: 0 794 1371
Size: 794 577
BsrBI CCG'CTC
Cuts at: 0 965 990 1371
Size: 965 25 381
Fragments arranged by size:
965 381 25
BstYI r'GATC_y
Cuts at: 0 194 791 1371
Size: 194 597 580
Fragments arranged by size:
597 580 194
BtsI GCAGTG_nn'
Cuts at: 0 47 935 1371
Size: 47 888 436
Fragments arranged by size:
888 436 47
EaeI y'GGCC_r
Cuts at: 0 1043 1313 1371
Size: 1043 270 58
Fragments arranged by size:
1043 270 58
EarI CTCTTCn'nnn_
Cuts at: 0 29 359 512 677 952 955 1371
Size: 29 330 153 165 275 3 416
Fragments arranged by size:
416 330 275 165 153 29 3
EcoICRI GAG'CTC
Cuts at: 0 234 1371
Size: 234 1137
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 247 539 737 994 1044 1093 1131 1200
Size: 247 292 198 257 50 49 38 69
Cuts at: 1200 1210 1321 1351 1371
Size: 10 111 30 20
Fragments arranged by size:
292 257 247 198 111 69 50 49
38 30 20 10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 619 1371
Size: 619 752
EcoO109I rG'GnC_Cy
Cuts at: 0 449 456 504 612 645 745 867 1152
Size: 449 7 48 108 33 100 122 285
Cuts at: 1152 1153 1371
Size: 1 218
Fragments arranged by size:
449 285 218 122 108 100 48 33
7 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1140 1371
Size: 1140 231
HaeII r_GCGC'y
Cuts at: 0 260 1371
Size: 260 1111
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 258 290 295 327 358 390 796 828
Size: 258 32 5 32 31 32 406 32
Cuts at: 828 1294 1326 1371
Size: 466 32 45
Fragments arranged by size:
466 406 258 45 32 32 32 32
32 31 5
KasI G'GCGC_C
Cuts at: 0 256 1371
Size: 256 1115
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 146 794 1371
Size: 146 648 577
Fragments arranged by size:
648 577 146
MscI TGG'CCA
Cuts at: 0 1045 1371
Size: 1045 326
MslI CAynn'nnrTG
Cuts at: 0 754 1371
Size: 754 617
MspA1I CmG'CkG
Cuts at: 0 13 293 1041 1167 1350 1371
Size: 13 280 748 126 183 21
Fragments arranged by size:
748 280 183 126 21 13
NarI GG'CG_CC
Cuts at: 0 257 1371
Size: 257 1114
NsiI A_TGCA'T
Cuts at: 0 106 1371
Size: 106 1265
PasI CC'CwG_GG
Cuts at: 0 508 1156 1371
Size: 508 648 215
Fragments arranged by size:
648 508 215
PflMI CCAn_nnn'nTGG
Cuts at: 0 1004 1022 1299 1371
Size: 1004 18 277 72
Fragments arranged by size:
1004 277 72 18
PfoI T'CCnGG_A
Cuts at: 0 461 1371
Size: 461 910
PpuMI rG'GwC_Cy
Cuts at: 0 867 1371
Size: 867 504
PspOMI G'GGCC_C
Cuts at: 0 1130 1152 1371
Size: 1130 22 219
Fragments arranged by size:
1130 219 22
PstI C_TGCA'G
Cuts at: 0 1355 1371
Size: 1355 16
PvuII CAG'CTG
Cuts at: 0 1041 1167 1350 1371
Size: 1041 126 183 21
Fragments arranged by size:
1041 183 126 21
SacI G_AGCT'C
Cuts at: 0 236 1371
Size: 236 1135
SapI GCTCTTCn'nnn_
Cuts at: 0 29 359 955 1371
Size: 29 330 596 416
Fragments arranged by size:
596 416 330 29
SfcI C'TryA_G
Cuts at: 0 752 1351 1371
Size: 752 599 20
Fragments arranged by size:
752 599 20
SfoI GGC'GCC
Cuts at: 0 258 1371
Size: 258 1113
SmaI CCC'GGG
Cuts at: 0 1135 1195 1371
Size: 1135 60 176
Fragments arranged by size:
1135 176 60
SmlI C'TyrA_G
Cuts at: 0 322 1207 1371
Size: 322 885 164
Fragments arranged by size:
885 322 164
SrfI GCCC'GGGC
Cuts at: 0 1135 1371
Size: 1135 236
StuI AGG'CCT
Cuts at: 0 944 1371
Size: 944 427
StyI C'CwwG_G
Cuts at: 0 252 260 377 407 482 777 998 1046
Size: 252 8 117 30 75 295 221 48
Cuts at: 1046 1371
Size: 325
Fragments arranged by size:
325 295 252 221 117 75 48 30
8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 562 800 1371
Size: 562 238 571
Fragments arranged by size:
571 562 238
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 537 569 1371
Size: 537 32 802
Fragments arranged by size:
802 537 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 384 775 1371
Size: 384 391 596
Fragments arranged by size:
596 391 384
XmaI C'CCGG_G
Cuts at: 0 1133 1193 1371
Size: 1133 60 178
Fragments arranged by size:
1133 178 60
Enzymes that do cut:
AcuI AleI AlfI AlwNI ApaI ApoI AvaI BaeI
BamHI BanI BanII BbeI BbvCI BciVI BfrBI BglI
Bme1580I BplI BpmI Bpu10I BpuEI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BsrBI
BstYI BtsI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I
FalI HaeII Hin4I KasI MmeI MscI MslI MspA1I
NarI NsiI PasI PflMI PfoI PpuMI PspOMI PstI
PvuII SacI SapI SfcI SfoI SmaI SmlI SrfI
StuI StyI TaqII TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AloI ApaLI AscI AseI AsiSI AvrII
BbsI BcgI BclI BglII BlpI BmgBI BmrI BmtI
BsaI BsaAI BsaBI BsiWI BsmI BsmBI BspHI BspMI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI
DraIII DrdI EagI EciI EcoRI EcoRV FseI FspI
FspAI HincII HindIII HpaI KpnI MfeI MluI NaeI
NcoI NdeI NgoMIV NheI NotI NruI NspI PacI
PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI
SfiI SgfI SgrAI SnaBI SpeI SphI SspI SwaI
TatI Tth111I XbaI XhoI XmnI ZraI