Restriction map for
pFN21AE4434
(Linear) (Six-base) MAPSORT of: KIEE4434.seq Check: 3239 from: 1 to: 1356
KIEE4434.orf
With 182 enzymes: SgfI *
March 22, 2012 18:01 ..
AccI GT'mk_AC
Cuts at: 0 829 1356
Size: 829 527
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 601 1356
Size: 601 755
AflII C'TTAA_G
Cuts at: 0 1288 1356
Size: 1288 68
AflIII A'CryG_T
Cuts at: 0 867 1356
Size: 867 489
AlwNI CAG_nnn'CTG
Cuts at: 0 699 1356
Size: 699 657
ApaLI G'TGCA_C
Cuts at: 0 935 1356
Size: 935 421
ApoI r'AATT_y
Cuts at: 0 232 1356
Size: 232 1124
AvaI C'yCGr_G
Cuts at: 0 524 752 1356
Size: 524 228 604
Fragments arranged by size:
604 524 228
BamHI G'GATC_C
Cuts at: 0 814 1356
Size: 814 542
BanI G'GyrC_C
Cuts at: 0 97 307 1356
Size: 97 210 1049
Fragments arranged by size:
1049 210 97
BanII G_rGCy'C
Cuts at: 0 998 1040 1356
Size: 998 42 316
Fragments arranged by size:
998 316 42
BbsI GAAGACnn'nnnn_
Cuts at: 0 83 689 777 1117 1356
Size: 83 606 88 340 239
Fragments arranged by size:
606 340 239 88 83
BglII A'GATC_T
Cuts at: 0 287 1356
Size: 287 1069
Bme1580I G_kGCm'C
Cuts at: 0 356 565 875 939 1341 1356
Size: 356 209 310 64 402 15
Fragments arranged by size:
402 356 310 209 64 15
BmrI ACTGGGnnnn_n'
Cuts at: 0 894 1356
Size: 894 462
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1120 1126 1327 1356
Size: 1120 6 201 29
Fragments arranged by size:
1120 201 29 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 312 1356
Size: 312 1044
BsaAI yAC'GTr
Cuts at: 0 1161 1356
Size: 1161 195
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 181 1356
Size: 181 1175
BseYI C'CCAG_C
Cuts at: 0 674 1033 1280 1356
Size: 674 359 247 76
Fragments arranged by size:
674 359 247 76
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 777 1356
Size: 777 579
BsiHKAI G_wGCw'C
Cuts at: 0 939 1356
Size: 939 417
Bsp1286I G_dGCh'C
Cuts at: 0 356 565 875 939 998 1040 1341 1356
Size: 356 209 310 64 59 42 301 15
Fragments arranged by size:
356 310 301 209 64 59 42 15
BsrBI CCG'CTC
Cuts at: 0 418 1356
Size: 418 938
BsrDI GCAATG_nn'
Cuts at: 0 488 1356
Size: 488 868
BssSI C'ACGA_G
Cuts at: 0 329 1356
Size: 329 1027
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1030 1356
Size: 1030 326
BstXI CCAn_nnnn'nTGG
Cuts at: 0 916 1356
Size: 916 440
BstYI r'GATC_y
Cuts at: 0 287 450 814 1356
Size: 287 163 364 542
Fragments arranged by size:
542 364 287 163
BstZ17I GTA'TAC
Cuts at: 0 830 1356
Size: 830 526
BtgI C'CryG_G
Cuts at: 0 507 1356
Size: 507 849
BtsI GCAGTG_nn'
Cuts at: 0 5 789 1341 1356
Size: 5 784 552 15
Fragments arranged by size:
784 552 15 5
DraI TTT'AAA
Cuts at: 0 215 447 1356
Size: 215 232 909
Fragments arranged by size:
909 232 215
DrdI GACnn_nn'nnGTC
Cuts at: 0 582 1356
Size: 582 774
EaeI y'GGCC_r
Cuts at: 0 143 1356
Size: 143 1213
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 709 1356
Size: 709 647
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 601 1120 1126 1327 1356
Size: 601 519 6 201 29
Fragments arranged by size:
601 519 201 29 6
HindIII A'AGCT_T
Cuts at: 0 1087 1356
Size: 1087 269
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 750 1356
Size: 750 606
MscI TGG'CCA
Cuts at: 0 145 1356
Size: 145 1211
MspA1I CmG'CkG
Cuts at: 0 678 1356
Size: 678 678
NcoI C'CATG_G
Cuts at: 0 507 1356
Size: 507 849
NdeI CA'TA_TG
Cuts at: 0 1026 1356
Size: 1026 330
NspI r_CATG'y
Cuts at: 0 871 1356
Size: 871 485
PciI A'CATG_T
Cuts at: 0 867 1356
Size: 867 489
PfoI T'CCnGG_A
Cuts at: 0 14 1356
Size: 14 1342
PspXI vC'TCGA_Gb
Cuts at: 0 524 1356
Size: 524 832
PstI C_TGCA'G
Cuts at: 0 704 1356
Size: 704 652
PvuII CAG'CTG
Cuts at: 0 678 1356
Size: 678 678
ScaI AGT'ACT
Cuts at: 0 646 1066 1356
Size: 646 420 290
Fragments arranged by size:
646 420 290
SfcI C'TryA_G
Cuts at: 0 700 1356
Size: 700 656
SmlI C'TyrA_G
Cuts at: 0 291 524 1288 1356
Size: 291 233 764 68
Fragments arranged by size:
764 291 233 68
StyI C'CwwG_G
Cuts at: 0 507 1184 1356
Size: 507 677 172
Fragments arranged by size:
677 507 172
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 256 754 922 1267 1356
Size: 256 498 168 345 89
Fragments arranged by size:
498 345 256 168 89
TatI w'GTAC_w
Cuts at: 0 614 644 800 1064 1356
Size: 614 30 156 264 292
Fragments arranged by size:
614 292 264 156 30
XbaI T'CTAG_A
Cuts at: 0 199 1356
Size: 199 1157
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 12 378 525 1356
Size: 12 366 147 831
Fragments arranged by size:
831 366 147 12
XhoI C'TCGA_G
Cuts at: 0 524 1356
Size: 524 832
Enzymes that do cut:
AccI AcuI AflII AflIII AlwNI ApaLI ApoI AvaI
BamHI BanI BanII BbsI BglII Bme1580I BmrI BpmI
BpuEI BsaAI BseRI BseYI BsgI BsiHKAI Bsp1286I BsrBI
BsrDI BssSI BstAPI BstXI BstYI BstZ17I BtgI BtsI
DraI DrdI EaeI EciI Eco57MI HindIII MmeI MscI
MspA1I NcoI NdeI NspI PciI PfoI PspXI PstI
PvuII ScaI SfcI SmlI StyI TaqII TatI XbaI
XcmI XhoI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AhdI AleI
AlfI AloI ApaI AscI AseI AsiSI AvrII BaeI
BbeI BbvCI BcgI BciVI BclI BfrBI BglI BlpI
BmgBI BmtI BplI Bpu10I BsaI BsaBI BsaHI BsaWI
BsaXI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI
BsrFI BsrGI BssHII BstBI BstEII Bsu36I BtgZI ClaI
CspCI DraIII EagI EarI EcoICRI EcoNI EcoO109I EcoRI
EcoRV FalI FseI FspI FspAI HaeII Hin4I HincII
HpaI KasI KpnI MfeI MluI MslI NaeI NarI
NgoMIV NheI NotI NruI NsiI PacI PasI PflMI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI TstI Tth111I XmaI
XmnI ZraI