Restriction map for
pFN21AE4432
(Linear) (Six-base) MAPSORT of: KIEE4432.seq Check: 6698 from: 1 to: 1353
KIEE4432.orf
With 182 enzymes: SgfI *
March 22, 2012 18:00 ..
AatII G_ACGT'C
Cuts at: 0 149 1353
Size: 149 1204
AccI GT'mk_AC
Cuts at: 0 697 1353
Size: 697 656
AflIII A'CryG_T
Cuts at: 0 1235 1353
Size: 1235 118
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1222 1254 1353
Size: 1222 32 99
Fragments arranged by size:
1222 99 32
AlwNI CAG_nnn'CTG
Cuts at: 0 439 1353
Size: 439 914
ApaI G_GGCC'C
Cuts at: 0 1057 1353
Size: 1057 296
AvaI C'yCGr_G
Cuts at: 0 97 185 212 1353
Size: 97 88 27 1141
Fragments arranged by size:
1141 97 88 27
BamHI G'GATC_C
Cuts at: 0 1016 1353
Size: 1016 337
BanI G'GyrC_C
Cuts at: 0 1339 1353
Size: 1339 14
BanII G_rGCy'C
Cuts at: 0 1057 1127 1353
Size: 1057 70 226
Fragments arranged by size:
1057 226 70
BbsI GAAGACnn'nnnn_
Cuts at: 0 865 1353
Size: 865 488
BciVI GTATCCnnnnn_n'
Cuts at: 0 491 1353
Size: 491 862
BglI GCCn_nnn'nGGC
Cuts at: 0 38 101 1353
Size: 38 63 1252
Fragments arranged by size:
1252 63 38
Bme1580I G_kGCm'C
Cuts at: 0 133 1057 1342 1353
Size: 133 924 285 11
Fragments arranged by size:
924 285 133 11
BmrI ACTGGGnnnn_n'
Cuts at: 0 318 454 1353
Size: 318 136 899
Fragments arranged by size:
899 318 136
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1106 1353
Size: 1106 247
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1234 1353
Size: 1234 119
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 559 1353
Size: 559 794
BsaI GGTCTCn'nnnn_
Cuts at: 0 920 1353
Size: 920 433
BsaHI Gr'CG_yC
Cuts at: 0 146 1353
Size: 146 1207
BsaWI w'CCGG_w
Cuts at: 0 632 1353
Size: 632 721
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1222 1252 1315 1345 1353
Size: 1222 30 63 30 8
Fragments arranged by size:
1222 63 30 30 8
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 127 305 308 556 1143 1353
Size: 127 178 3 248 587 210
Fragments arranged by size:
587 248 210 178 127 3
BseYI C'CCAG_C
Cuts at: 0 424 1353
Size: 424 929
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 517 637 1303 1353
Size: 517 120 666 50
Fragments arranged by size:
666 517 120 50
BsiHKAI G_wGCw'C
Cuts at: 0 176 1353
Size: 176 1177
BsmI GAATG_Cn'
Cuts at: 0 597 1353
Size: 597 756
Bsp1286I G_dGCh'C
Cuts at: 0 133 176 1057 1127 1342 1353
Size: 133 43 881 70 215 11
Fragments arranged by size:
881 215 133 70 43 11
BspHI T'CATG_A
Cuts at: 0 806 878 1127 1353
Size: 806 72 249 226
Fragments arranged by size:
806 249 226 72
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1106 1353
Size: 1106 247
BsrDI GCAATG_nn'
Cuts at: 0 419 1353
Size: 419 934
BsrFI r'CCGG_y
Cuts at: 0 811 1353
Size: 811 542
BstXI CCAn_nnnn'nTGG
Cuts at: 0 870 1353
Size: 870 483
BstYI r'GATC_y
Cuts at: 0 338 1016 1353
Size: 338 678 337
Fragments arranged by size:
678 338 337
BtgI C'CryG_G
Cuts at: 0 790 863 1048 1353
Size: 790 73 185 305
Fragments arranged by size:
790 305 185 73
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 551 753 936 1353
Size: 551 202 183 417
Fragments arranged by size:
551 417 202 183
BtsI GCAGTG_nn'
Cuts at: 0 942 1353
Size: 942 411
ClaI AT'CG_AT
Cuts at: 0 1295 1353
Size: 1295 58
EaeI y'GGCC_r
Cuts at: 0 845 1034 1133 1310 1318 1353
Size: 845 189 99 177 8 35
Fragments arranged by size:
845 189 177 99 35 8
EarI CTCTTCn'nnn_
Cuts at: 0 1165 1353
Size: 1165 188
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1234 1353
Size: 1234 119
EcoO109I rG'GnC_Cy
Cuts at: 0 1053 1054 1353
Size: 1053 1 299
Fragments arranged by size:
1053 299 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 165 1266 1353
Size: 165 1101 87
Fragments arranged by size:
1101 165 87
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 323 355 1158 1190 1315 1347 1353
Size: 323 32 803 32 125 32 6
Fragments arranged by size:
803 323 125 32 32 32 6
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 260 1353
Size: 260 1093
MscI TGG'CCA
Cuts at: 0 847 1036 1135 1353
Size: 847 189 99 218
Fragments arranged by size:
847 218 189 99
MslI CAynn'nnrTG
Cuts at: 0 1069 1353
Size: 1069 284
MspA1I CmG'CkG
Cuts at: 0 72 317 690 1121 1353
Size: 72 245 373 431 232
Fragments arranged by size:
431 373 245 232 72
NspI r_CATG'y
Cuts at: 0 1239 1353
Size: 1239 114
PasI CC'CwG_GG
Cuts at: 0 327 1353
Size: 327 1026
PciI A'CATG_T
Cuts at: 0 1235 1353
Size: 1235 118
PflMI CCAn_nnn'nTGG
Cuts at: 0 843 1353
Size: 843 510
PfoI T'CCnGG_A
Cuts at: 0 869 1019 1211 1353
Size: 869 150 192 142
Fragments arranged by size:
869 192 150 142
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1222 1254 1353
Size: 1222 32 99
Fragments arranged by size:
1222 99 32
PshAI GACnn'nnGTC
Cuts at: 0 865 1353
Size: 865 488
PspOMI G'GGCC_C
Cuts at: 0 1053 1353
Size: 1053 300
PstI C_TGCA'G
Cuts at: 0 896 1353
Size: 896 457
PvuII CAG'CTG
Cuts at: 0 72 690 1353
Size: 72 618 663
Fragments arranged by size:
663 618 72
SapI GCTCTTCn'nnn_
Cuts at: 0 1165 1353
Size: 1165 188
SfcI C'TryA_G
Cuts at: 0 22 892 1353
Size: 22 870 461
Fragments arranged by size:
870 461 22
SmaI CCC'GGG
Cuts at: 0 187 214 1353
Size: 187 27 1139
Fragments arranged by size:
1139 187 27
SmlI C'TyrA_G
Cuts at: 0 574 1353
Size: 574 779
StuI AGG'CCT
Cuts at: 0 1207 1353
Size: 1207 146
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1006 1353
Size: 1006 347
XbaI T'CTAG_A
Cuts at: 0 393 1353
Size: 393 960
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 906 1068 1353
Size: 906 162 285
Fragments arranged by size:
906 285 162
XmaI C'CCGG_G
Cuts at: 0 185 212 1353
Size: 185 27 1141
Fragments arranged by size:
1141 185 27
ZraI GAC'GTC
Cuts at: 0 147 1353
Size: 147 1206
Enzymes that do cut:
AatII AccI AflIII AloI AlwNI ApaI AvaI BamHI
BanI BanII BbsI BciVI BglI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI BsrFI
BstXI BstYI BtgI BtgZI BtsI ClaI EaeI EarI
Eco57MI EcoO109I FalI Hin4I MmeI MscI MslI MspA1I
NspI PasI PciI PflMI PfoI PpiI PshAI PspOMI
PstI PvuII SapI SfcI SmaI SmlI StuI TaqII
XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AcuI AfeI AflII AgeI AhdI
AleI AlfI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BbeI BbvCI BcgI BclI BfrBI BglII BlpI
BmgBI BmtI Bpu10I BsaAI BsaBI BsiEI BsiWI BsmBI
BspEI BsrBI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstZ17I Bsu36I CspCI DraI DraIII DrdI EagI EciI
EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI HaeII
HincII HindIII HpaI KasI KpnI MfeI MluI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PmeI PmlI PpuMI PsiI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI StyI SwaI TatI TstI Tth111I XhoI XmnI