Restriction map for
pFN21AE4422
(Linear) (Six-base) MAPSORT of: KIEE4422.seq Check: 1952 from: 1 to: 1311
KIEE4422.orf
With 182 enzymes: SgfI *
March 22, 2012 17:58 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 151 1311
Size: 151 1160
AccI GT'mk_AC
Cuts at: 0 149 1311
Size: 149 1162
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 673 1311
Size: 673 638
AflIII A'CryG_T
Cuts at: 0 769 1118 1311
Size: 769 349 193
Fragments arranged by size:
769 349 193
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 115 1246 1311
Size: 115 1131 65
Fragments arranged by size:
1131 115 65
ApoI r'AATT_y
Cuts at: 0 208 267 487 781 815 1311
Size: 208 59 220 294 34 496
Fragments arranged by size:
496 294 220 208 59 34
AseI AT'TA_AT
Cuts at: 0 443 644 1311
Size: 443 201 667
Fragments arranged by size:
667 443 201
BanI G'GyrC_C
Cuts at: 0 630 1311
Size: 630 681
BanII G_rGCy'C
Cuts at: 0 79 1311
Size: 79 1232
BbsI GAAGACnn'nnnn_
Cuts at: 0 467 1311
Size: 467 844
Bme1580I G_kGCm'C
Cuts at: 0 971 1311
Size: 971 340
BmrI ACTGGGnnnn_n'
Cuts at: 0 1190 1311
Size: 1190 121
BsaI GGTCTCn'nnnn_
Cuts at: 0 23 1311
Size: 23 1288
BseYI C'CCAG_C
Cuts at: 0 24 1311
Size: 24 1287
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 701 1311
Size: 701 610
BsiEI CG_ry'CG
Cuts at: 0 141 1311
Size: 141 1170
Bsp1286I G_dGCh'C
Cuts at: 0 79 971 1311
Size: 79 892 340
Fragments arranged by size:
892 340 79
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 151 1311
Size: 151 1160
BsrGI T'GTAC_A
Cuts at: 0 110 1311
Size: 110 1201
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1257 1311
Size: 1257 54
BstEII G'GTnAC_C
Cuts at: 0 277 1311
Size: 277 1034
BstZ17I GTA'TAC
Cuts at: 0 150 1311
Size: 150 1161
BtsI GCAGTG_nn'
Cuts at: 0 563 983 1311
Size: 563 420 328
Fragments arranged by size:
563 420 328
DraIII CAC_nnn'GTG
Cuts at: 0 1176 1311
Size: 1176 135
EaeI y'GGCC_r
Cuts at: 0 524 1311
Size: 524 787
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 673 1311
Size: 673 638
EcoNI CCTnn'n_nnAGG
Cuts at: 0 402 655 1311
Size: 402 253 656
Fragments arranged by size:
656 402 253
EcoO109I rG'GnC_Cy
Cuts at: 0 347 1311
Size: 347 964
EcoRI G'AATT_C
Cuts at: 0 487 1311
Size: 487 824
HaeII r_GCGC'y
Cuts at: 0 585 1311
Size: 585 726
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 729 761 1311
Size: 729 32 550
Fragments arranged by size:
729 550 32
HincII GTy'rAC
Cuts at: 0 1154 1311
Size: 1154 157
MfeI C'AATT_G
Cuts at: 0 4 733 1311
Size: 4 729 578
Fragments arranged by size:
729 578 4
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1092 1311
Size: 1092 219
MscI TGG'CCA
Cuts at: 0 526 1311
Size: 526 785
MslI CAynn'nnrTG
Cuts at: 0 1082 1228 1311
Size: 1082 146 83
Fragments arranged by size:
1082 146 83
MspA1I CmG'CkG
Cuts at: 0 358 1311
Size: 358 953
NspI r_CATG'y
Cuts at: 0 773 1053 1122 1233 1311
Size: 773 280 69 111 78
Fragments arranged by size:
773 280 111 78 69
PciI A'CATG_T
Cuts at: 0 769 1118 1311
Size: 769 349 193
Fragments arranged by size:
769 349 193
PflMI CCAn_nnn'nTGG
Cuts at: 0 229 1311
Size: 229 1082
PpuMI rG'GwC_Cy
Cuts at: 0 347 1311
Size: 347 964
PstI C_TGCA'G
Cuts at: 0 459 1311
Size: 459 852
PvuI CG_AT'CG
Cuts at: 0 141 1311
Size: 141 1170
PvuII CAG'CTG
Cuts at: 0 358 1311
Size: 358 953
SanDI GG'GwC_CC
Cuts at: 0 347 1311
Size: 347 964
SfcI C'TryA_G
Cuts at: 0 455 1292 1311
Size: 455 837 19
Fragments arranged by size:
837 455 19
SspI AAT'ATT
Cuts at: 0 196 1311
Size: 196 1115
StyI C'CwwG_G
Cuts at: 0 125 1103 1311
Size: 125 978 208
Fragments arranged by size:
978 208 125
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 302 1311
Size: 302 1009
TatI w'GTAC_w
Cuts at: 0 110 1311
Size: 110 1201
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1049 1081 1311
Size: 1049 32 230
Fragments arranged by size:
1049 230 32
Tth111I GACn'n_nGTC
Cuts at: 0 53 1311
Size: 53 1258
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1177 1311
Size: 1177 134
XmnI GAAnn'nnTTC
Cuts at: 0 863 1311
Size: 863 448
Enzymes that do cut:
AarI AccI AcuI AflIII AlfI ApoI AseI BanI
BanII BbsI Bme1580I BmrI BsaI BseYI BsgI BsiEI
Bsp1286I BspMI BsrGI BstAPI BstEII BstZ17I BtsI DraIII
EaeI Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I HincII
MfeI MmeI MscI MslI MspA1I NspI PciI PflMI
PpuMI PstI PvuI PvuII SanDI SfcI SspI StyI
TaqII TatI TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
AloI AlwNI ApaI ApaLI AscI AsiSI AvaI AvrII
BaeI BamHI BbeI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI BmgBI BmtI BplI BpmI Bpu10I
BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BsiHKAI
BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrFI
BssHII BssSI BstBI BstXI BstYI Bsu36I BtgI BtgZI
ClaI CspCI DraI DrdI EagI EarI EciI EcoICRI
EcoRV FalI FseI FspI FspAI HindIII HpaI KasI
KpnI MluI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI RsrII SacI
SacII SalI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
StuI SwaI XbaI XhoI XmaI ZraI